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-基本情報
登録情報 | データベース: PDB / ID: 5o6v | ||||||||||||
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タイトル | The cryo-EM structure of Tick-borne encephalitis virus complexed with Fab fragment of neutralizing antibody 19/1786 | ||||||||||||
要素 |
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キーワード | VIRUS (ウイルス) / tick-borne encephalitis virus / Fab 19/1786 / cryo-EM (低温電子顕微鏡法) | ||||||||||||
機能・相同性 | 機能・相同性情報 フラビビリン / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / カプシド / nucleoside-triphosphate phosphatase / double-stranded RNA binding / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity ...フラビビリン / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / カプシド / nucleoside-triphosphate phosphatase / double-stranded RNA binding / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / host cell endoplasmic reticulum membrane / host cell perinuclear region of cytoplasm / protein dimerization activity / ヘリカーゼ / induction by virus of host autophagy / RNA依存性RNAポリメラーゼ / viral RNA genome replication / RNA-dependent RNA polymerase activity / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / エンベロープ (ウイルス) / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / virion attachment to host cell / virion membrane / structural molecule activity / ATP hydrolysis activity / タンパク質分解 / extracellular region / ATP binding / 生体膜 / metal ion binding 類似検索 - 分子機能 | ||||||||||||
生物種 | Tick-borne encephalitis virus (ダニ媒介脳炎ウイルス) Mus musculus (ハツカネズミ) | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | ||||||||||||
データ登録者 | Fuzik, T. / Plevka, P. | ||||||||||||
資金援助 | ドイツ, チェコ, 3件
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引用 | ジャーナル: Nat Commun / 年: 2018 タイトル: Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. 著者: Tibor Füzik / Petra Formanová / Daniel Růžek / Kentaro Yoshii / Matthias Niedrig / Pavel Plevka / 要旨: Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. ...Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
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PDBx/mmCIF形式 | 5o6v.cif.gz | 479.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb5o6v.ent.gz | 402.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 5o6v.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/o6/5o6v ftp://data.pdbj.org/pub/pdb/validation_reports/o6/5o6v | HTTPS FTP |
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非結晶学的対称性 (NCS) | NCS oper:
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