+Open data
-Basic information
Entry | Database: PDB / ID: 8iwk | ||||||
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Title | ABCG25 Wild Type purified with DDM plus CHS in ABA-bound state | ||||||
Components | ABC transporter G family member 25 | ||||||
Keywords | TRANSPORT PROTEIN / ABA / ABCG / plant hormone | ||||||
Function / homology | Function and homology information negative regulation of post-embryonic development / abscisic acid transport / export from cell / intercellular transport / response to abscisic acid / abscisic acid-activated signaling pathway / efflux transmembrane transporter activity / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / response to cold ...negative regulation of post-embryonic development / abscisic acid transport / export from cell / intercellular transport / response to abscisic acid / abscisic acid-activated signaling pathway / efflux transmembrane transporter activity / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / response to cold / transmembrane transport / response to heat / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | ||||||
Authors | Sun, L. / Liu, X. / Ying, W. / Liao, L. / Wei, H. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: ABCG25 Wild Type purified with DDM plus CHS in ABA-bound state Authors: Sun, L. / Liu, X. / Ying, W. / Liao, L. / Wei, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8iwk.cif.gz | 194.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8iwk.ent.gz | 155.1 KB | Display | PDB format |
PDBx/mmJSON format | 8iwk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/iw/8iwk ftp://data.pdbj.org/pub/pdb/validation_reports/iw/8iwk | HTTPS FTP |
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-Related structure data
Related structure data | 35769MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 72983.867 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: ABCG25 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q84TH5 #2: Chemical | ChemComp-A8S / ( | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: ABCG25 from Arabidopsis thaliana / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: DIFFRACTION / Nominal defocus max: 2300 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
EM software | Name: PHENIX / Version: 1.13_2998: / Category: model refinement |
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CTF correction | Type: NONE |
3D reconstruction | Resolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 322151 / Symmetry type: POINT |