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- PDB-8cbe: SARS-CoV-2 Delta-RBD complexed with BA.4/5-2 and Beta-49 Fabs -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 8cbe
TitleSARS-CoV-2 Delta-RBD complexed with BA.4/5-2 and Beta-49 Fabs
Components
  • BA.4/5-2 heavy chain
  • BA.4/5-2 light chain
  • Beta-49 heavy chain
  • Beta-49 light chain
  • Spike protein S1
KeywordsVIRAL PROTEIN / SARS-CoV-2 / BA.4 mAb / BA.5 mAb / RBD / BA.4/5-1 / BA.4/5-2 / BA.4/5-5 / Beta-49 / VIRAL PROTEIN/Immune system
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.16 Å
AuthorsZhou, D. / Ren, J. / Stuart, D.I.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
CAMS Innovation Fund for Medical Sciences (CIFMS)2018-I2M-2-002 United Kingdom
Medical Research Council (MRC, United Kingdom)MR/N00065X/1 United Kingdom
CitationJournal: Nat Commun / Year: 2024
Title: Emerging variants develop total escape from potent monoclonal antibodies induced by BA.4/5 infection.
Authors: Liu, C. / Das, R. / Dijokaite-Guraliuc, A. / Zhou, D. / Mentzer, A.J. / Supasa, P. / Selvaraj, M. / Duyvesteyn, H.M.E. / Ritter, T.G. / Temperton, N. / Klenerman, P. / Dunachie, S.J. / ...Authors: Liu, C. / Das, R. / Dijokaite-Guraliuc, A. / Zhou, D. / Mentzer, A.J. / Supasa, P. / Selvaraj, M. / Duyvesteyn, H.M.E. / Ritter, T.G. / Temperton, N. / Klenerman, P. / Dunachie, S.J. / Paterson, N.G. / Williams, M.A. / Hall, D.R. / Fry, E.E. / Mongkolsapaya, J. / Ren, J. / Stuart, D.I. / Screaton, G.R.
History
DepositionJan 25, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 7, 2024Provider: repository / Type: Initial release
Revision 1.1May 8, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: BA.4/5-2 heavy chain
L: BA.4/5-2 light chain
E: Spike protein S1
B: Beta-49 light chain
A: Beta-49 heavy chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)118,4136
Polymers117,6815
Non-polymers7331
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12200 Å2
ΔGint-60 kcal/mol
Surface area45950 Å2
MethodPISA
Unit cell
Length a, b, c (Å)179.861, 147.022, 52.197
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x+1/2,y+1/2,-z
#4: -x,-y,z

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Components

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Antibody , 4 types, 4 molecules HLBA

#1: Antibody BA.4/5-2 heavy chain


Mass: 24359.309 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody BA.4/5-2 light chain


Mass: 23387.990 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody Beta-49 light chain


Mass: 23566.121 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#5: Antibody Beta-49 heavy chain


Mass: 23431.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Protein / Sugars , 2 types, 2 molecules E

#3: Protein Spike protein S1


Mass: 22935.734 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#6: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 732.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1221m-1a_1-5]/1-1-2-3/a4-b1_a6-d1_b4-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.93 Å3/Da / Density % sol: 58.05 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 0.1 M sodium citrate tribasic dihydrate pH 5.0 and 18% (w/v) PEG 20000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.95373 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Nov 25, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95373 Å / Relative weight: 1
ReflectionResolution: 3.16→60 Å / Num. obs: 24651 / % possible obs: 100 % / Redundancy: 13.3 % / Biso Wilson estimate: 101.13 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.345 / Rpim(I) all: 0.098 / Net I/σ(I): 6.1
Reflection shellResolution: 3.16→3.21 Å / Mean I/σ(I) obs: 0.5 / Num. unique obs: 1210 / CC1/2: 0.322 / % possible all: 99.2

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Processing

Software
NameVersionClassification
GDA1.20.1_4487data collection
PHENIX1.20.1_4487refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7Q0G

7q0g


Resolution: 3.16→56.92 Å / SU ML: 0.4584 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 26.8599
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2657 1206 4.91 %
Rwork0.2262 23351 -
obs0.2282 24557 99.78 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 102.96 Å2
Refinement stepCycle: LAST / Resolution: 3.16→56.92 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8085 0 49 0 8134
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00238328
X-RAY DIFFRACTIONf_angle_d0.517811327
X-RAY DIFFRACTIONf_chiral_restr0.04251277
X-RAY DIFFRACTIONf_plane_restr0.00461451
X-RAY DIFFRACTIONf_dihedral_angle_d11.55122987
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.16-3.290.34941220.34192549X-RAY DIFFRACTION99.11
3.29-3.440.34241100.3172552X-RAY DIFFRACTION99.92
3.44-3.620.30291350.28782534X-RAY DIFFRACTION99.93
3.62-3.840.30191410.27532572X-RAY DIFFRACTION100
3.84-4.140.29991410.24722541X-RAY DIFFRACTION100
4.14-4.560.26551270.20252612X-RAY DIFFRACTION99.96
4.56-5.220.251400.192616X-RAY DIFFRACTION99.93
5.22-6.570.2691490.21182613X-RAY DIFFRACTION100
6.57-56.920.21451410.19632762X-RAY DIFFRACTION99.21
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.63825066280.561794064715-3.342703763685.79269740766-3.594985721743.59628684948-0.223780235649-0.2287758736451.26834390972-0.0153437085309-0.06395236488561.05709351473-0.789851109880.225208114156-0.120443097820.7732258152060.147026593287-0.05645087716060.53675709095-0.04906617110240.480023387826-9.07257.755-16.727
24.67027099852.877407483940.7182678119097.82205227454-2.095172067923.66382453722-0.5414593794050.8723051886130.8945464441720.3331801948621.423901710911.722335242060.03413272484620.0721523587497-0.2513072621240.874188747120.1046854105740.1182543020041.17367680134-0.1317287165170.999577891086-19.48250.776-13.911
33.261984755161.978401443290.4824259257077.2221338715-3.565807699654.00499843338-0.0289450608752-0.45682861624-0.375401969240.896635512186-0.455798476004-0.0786741671358-0.945676165132-0.259458529190.3077017813010.836246174252-0.0968571958390.01589972286491.02433598837-0.1572154561770.824241573245-7.7546.418-8.41
40.8793199269471.704277564630.7754742093146.34863854198-2.160906169153.64123026829-0.0203172689607-0.533702636987-0.2366004401830.2948512491310.6408578649150.675586946504-0.7225752516840.230668545342-0.1711831826711.187439485960.1635507190110.01929208280680.871586867677-0.2102673423730.953117696624-14.02854.326-7.015
56.183920343674.9878107515-1.414297409366.28243364098-3.403973065762.95713478348-0.712526199272-0.3532326611840.345393242239-0.1555911994210.06495609014430.347754654827-0.6478858288990.1531311809920.1500627948750.746368478774-0.0307902792770.1280227231971.04843318288-0.1972215565460.789877790559-3.85652.231-13.247
60.346983760621-0.446723636507-0.8229139550131.08402356963-1.350343880881.46054278704-0.1712829795070.544211377138-0.117998521269-0.06036457781990.03441287372220.1718501386840.102590156397-0.4586667592790.05346226774740.878544062282-0.184682807268-0.0640381679550.989353310045-0.1827545947950.78190438949-0.17351.532-25.162
74.44592550293-1.14937106188-2.214676093254.007313942251.572801938364.000100011110.67036801210.4896282606820.291626682073-0.447946278564-1.030683042640.0603309844709-0.840315109177-0.7382516842740.2060459669871.101683321690.0587660254199-0.09105369832220.8839646581460.01050300472620.7190831274648.39458.173-35.982
82.819494575220.209399649659-1.160724479434.681898135470.4457950291374.993475371660.6223191948841.099998577290.182445563027-0.678692360146-0.4696562935580.266945741166-0.364956690082-0.5092758828860.1191523463850.9356715343180.193535813442-0.1585560535571.007404283880.01408001667490.7108319656528.75559.57-43.608
92.696322974180.633674342367-2.580226414774.481437443111.307083387369.118250964281.254493974880.7949060029031.28866618011-0.215309323741-1.01877568751-0.210216255425-1.727575960470.4340873527560.1591566809591.35031174916-0.0535363930746-0.1315932010171.179856210130.204030698510.66484176249112.06666.751-44.944
106.86284214522-1.8032934846-1.887394241243.07150931701-3.454745533177.20083831961-0.3515735544270.0840303121476-0.87242136726-0.054292685182-0.9476456744710.5013463127951.198487277070.746929314260.6802160917990.823852999702-0.1686206509050.0591523336020.825603755586-0.06346074169330.8516785406953.99929.889-22.002
113.70788085677-0.544574884855-2.882807535962.93623323356-1.048321299166.42849281275-0.138173703870.136865284151-0.480847068908-0.0122783692635-0.3092236196140.3974078475230.724982371134-0.3547797643270.3441477987410.753739510885-0.1893516461490.1384533520450.823818035848-0.2319089163550.818990313635-3.38431.736-20.909
124.224433293443.42991971195-1.769529297128.823738410362.530929909741.609115608510.1754374972470.310125570587-0.00745142164031-0.303789040818-0.2397706495840.3045940121490.1760119340640.178836857406-0.0512871690530.9251656416450.0338132755517-0.0664506093940.9663845075890.06801436143380.68746274286221.03953.722-35.799
136.490730276811.429860453260.3734106618537.936592035350.9790273660423.25194994719-0.1034873222521.21674383065-0.7051599187340.3356193328990.5234345868240.324390589288-0.336793454050.611763062409-0.1823765212250.959908115715-0.03367627182440.04660379403031.04667289659-0.1322504463270.74377959129216.75947.449-32.584
144.6666988707-1.00687320191.343551166986.742491902721.117287649273.64721061409-0.03534636358860.4011362078471.026634458830.3511808320470.542587437694-1.28864732601-0.03562023345480.959446585764-0.284749855250.828811682779-0.1706965888680.03911431496611.078883871610.004613721881760.74861735912227.85458.539-32.601
156.792606452082.107302491923.234844545685.334391276014.788034896846.23374625576-0.3205445648970.5938807641170.0926804421117-0.1339183872330.602868790443-0.6718344198510.3499534326390.559864532162-0.4704706683090.6777491078660.0528432757720.008035609746751.159826464450.06212186082890.58868625136728.85448.224-38.94
162.35810735798-1.23802654829-1.270690377091.1093132127-2.452385234795.304742148120.141366246675-0.04467630808040.09426924231740.1838989639840.07338683977430.3238097229550.7524478122320.0088765180557-0.3600245477831.26131239513-0.2794967603270.07412998180741.08817177031-0.426125181460.995194567077-23.7029.6829.968
172.000864479541.958688012432.012231647886.36551648077-0.5282189173795.112146582760.107957995609-0.08097536055240.1035235597580.588121129585-0.146186768169-0.491698970753-0.2513768057730.3218917100720.02551007537550.896201144313-0.1523410741580.2428838288561.04454052443-0.4011615560121.15117179185-16.4218.3567.509
182.22555472802-1.33073666459-0.3684931415825.300688048640.8744205742072.834492969810.490691471322-0.1412903219650.3511742618890.0460616363737-0.97006512320.301624428446-0.158309897682-0.2933034853420.1930504302560.646018138804-0.09408883210330.2272860095530.960374960577-0.2679259121530.980529319799-25.10724.542-4.279
192.53056628621-0.9189080425390.7570430311572.95798674088-1.566560985274.482500268880.06002742661220.2997975722590.077423209840.0437142071549-0.2232155541820.219719577839-0.07130859854950.0484121737534-0.08655351977550.783429437053-0.2215347811540.05324659077840.992020677757-0.3814299443611.13511721694-24.64424.345-5.674
206.659906901931.75639462567-0.8414527809757.234941728291.470765118236.444762841680.1400810401960.06783899243970.889862027987-0.441874659134-0.1450353575060.605804314383-0.323397192288-0.0381084588695-0.0454930335070.6684981685420.0121951761033-0.02809667864340.555566940452-0.1135383855230.893027835675-38.604-6.057-2.874
213.928152107592.340088988410.2092269530517.368788057731.596427831756.75791884873-0.113552126744-0.1995093099510.74071295375-0.0292082984197-0.063328329911.08038485911-0.27265472060.6284767532210.0316241253440.8118269805740.1204111339390.03565466739160.679063880473-0.1234489915440.885203598424-38.758-5.887-1.893
220.0757033330568-0.0277709539323-0.09941995788331.680995899093.267572026066.10664868974-0.7865406625690.4470559932590.406032141099-1.09817944647-0.3793525936582.02070855935-0.239119961999-0.5567235738660.8940482822851.04035895687-0.0363518115449-0.1872942664380.70296450579-0.006491741321350.974013718218-45.545-20.068-16.874
232.178411246431.96973720369-0.09073942284717.55743223118-1.380264654443.6726364099-0.165869131921-0.0182597818388-0.01364508772030.5404676226390.3630443429990.327041332993-0.120541362836-0.108252525732-0.2223910637760.8470513442170.09051382506750.05276846983140.8637966262920.02559395945570.616442488253-47.253-41.003-10.705
242.145068588661.35308264032-0.224549902922.71269593473-2.342119270215.27190235622-0.650648809132-0.4547946409650.197947981495-0.6900821505641.52257525531.17431277480.924637725548-0.353970723299-0.3126302035191.028737828210.002106349316710.03108065203651.11096359966-0.03989861580241.0676991733-50.495-44.887-19.73
254.852288438723.800279791924.218200542447.946185508613.812566317264.858826424590.126626245309-0.443044017386-1.07958816791.03532835248-0.226479836068-1.259284998910.5816605950250.2981232896230.1489475460620.928899057162-0.110836390207-0.06503120555531.21080541306-0.1637884184810.749170400857-29.067-23.6915.111
263.683588440141.097736638912.706934204587.590550972390.1581149571313.951748307530.689384715473-0.9047475802750.3714555802940.959488363614-0.6098029558340.337018663171-0.174794294051-0.484061525992-0.08988047441880.859459342686-0.2298727252990.08687952995591.02907635426-0.2913789773790.833952120468-31.887-13.65114.332
274.785925860610.3385669417422.048456838028.509786390280.2471570032033.69001645211-0.4437506592790.6197769337281.685113458762.132908537110.0482769798481-0.00352335677326-0.4358191855830.319138227383-0.1108943438681.13071800042-0.202490928980.161438593641.34441225225-0.3622221720850.906685675314-36.257-11.53422.57
280.9724897972460.3736083024530.503045214348.989233032122.308948889871.08347939255-0.199763436217-0.438525779220.2328477864070.347191967195-0.2452339458670.3286209775180.703658812123-0.3267809902860.259214951891.13222941282-0.3292616824330.03326219955331.01080273398-0.1666914991620.701758949414-33.019-18.61917.212
291.691465373441.75348002646-1.224790800252.397134392710.3409561587861.1818379076-0.0828197658141-0.1282667977460.04455433076121.233631732480.593735106119-0.08697462694750.03381300037670.582106879638-0.4634794643490.849922163067-0.08286275778-0.0053095283040.905999440273-0.08161422240880.71159969634-30.877-20.5339.976
307.615063347941.38660821919-0.9136324126332.833484021444.41234815037.66327856939-0.9276460876941.58226908001-0.172946089997-0.652942499380.146461470653-0.0703223303052-3.394277570150.692207554874-0.05512023510730.9842923857090.0646316904165-0.1936541041550.957599638524-0.008367061523450.68593091395-36.419-46.043-14.22
315.249214344670.0937273647560.9930736966161.271247428272.272758681856.643424933590.4794193575480.116512968875-0.0467967496790.2329793387490.0733632067576-0.2306046677760.01397270979010.0836640913335-0.5365407023650.8530054425180.1032499902540.005032002725930.7028015582390.07633943022320.625610545795-34.429-37.432-4.96
326.404403675331.76268209814-0.9161950483963.647280437462.839190069872.52266271389-0.6550629152131.07600678846-0.7329076741210.3368134286030.08695268201540.2714586260031.59726511377-3.37102804947-0.3858897369520.9089983321210.07330205358790.1761740735360.870150061090.06159323025450.741180577557-34.631-39.021-13.443
336.826222653195.272660928363.04176600653.769559189383.549000647774.338498786820.4468215182210.133510385542-0.9125399593091.168413094280.804460261507-0.7868870303951.117711207321.18152286605-1.04897311670.8837633561060.103638704586-0.1083835830530.8306678718270.04661062034570.73817914457-26.823-43.573-7.587
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN H AND RESID 1:17 )H1 - 17
2X-RAY DIFFRACTION2( CHAIN H AND RESID 18:33 )H18 - 33
3X-RAY DIFFRACTION3( CHAIN H AND RESID 34:64 )H34 - 64
4X-RAY DIFFRACTION4( CHAIN H AND RESID 65:83 )H65 - 83
5X-RAY DIFFRACTION5( CHAIN H AND RESID 84:99 )H84 - 99
6X-RAY DIFFRACTION6( CHAIN H AND RESID 100:144 )H100 - 144
7X-RAY DIFFRACTION7( CHAIN H AND RESID 145:185 )H145 - 185
8X-RAY DIFFRACTION8( CHAIN H AND RESID 186:213 )H186 - 213
9X-RAY DIFFRACTION9( CHAIN H AND RESID 214:226 )H214 - 226
10X-RAY DIFFRACTION10( CHAIN L AND RESID 1:18 )L1 - 18
11X-RAY DIFFRACTION11( CHAIN L AND RESID 19:113 )L19 - 113
12X-RAY DIFFRACTION12( CHAIN L AND RESID 114:150 )L114 - 150
13X-RAY DIFFRACTION13( CHAIN L AND RESID 151:174 )L151 - 174
14X-RAY DIFFRACTION14( CHAIN L AND RESID 175:197 )L175 - 197
15X-RAY DIFFRACTION15( CHAIN L AND RESID 198:213 )L198 - 213
16X-RAY DIFFRACTION16( CHAIN E AND RESID 333:375 )E333 - 375
17X-RAY DIFFRACTION17( CHAIN E AND RESID 376:442 )E376 - 442
18X-RAY DIFFRACTION18( CHAIN E AND RESID 443:469 )E443 - 469
19X-RAY DIFFRACTION19( CHAIN E AND RESID 470:527 )E470 - 527
20X-RAY DIFFRACTION20( CHAIN B AND RESID 1:62 )B1 - 62
21X-RAY DIFFRACTION21( CHAIN B AND RESID 63:103 )B63 - 103
22X-RAY DIFFRACTION22( CHAIN B AND RESID 104:115 )B104 - 115
23X-RAY DIFFRACTION23( CHAIN B AND RESID 116:200 )B116 - 200
24X-RAY DIFFRACTION24( CHAIN B AND RESID 201:216 )B201 - 216
25X-RAY DIFFRACTION25( CHAIN A AND RESID 1:17 )A1 - 17
26X-RAY DIFFRACTION26( CHAIN A AND RESID 18:52 )A18 - 52
27X-RAY DIFFRACTION27( CHAIN A AND RESID 53:73 )A53 - 73
28X-RAY DIFFRACTION28( CHAIN A AND RESID 74:100 )A74 - 100
29X-RAY DIFFRACTION29( CHAIN A AND RESID 101:124 )A101 - 124
30X-RAY DIFFRACTION30( CHAIN A AND RESID 125:139 )A125 - 139
31X-RAY DIFFRACTION31( CHAIN A AND RESID 140:180 )A140 - 180
32X-RAY DIFFRACTION32( CHAIN A AND RESID 181:193 )A181 - 193
33X-RAY DIFFRACTION33( CHAIN A AND RESID 194:219 )A194 - 219

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