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Yorodumi- PDB-8b7i: Human HSP90 alpha ATP Binding Domain, ATP-lid open conformation, R60A -
+Open data
-Basic information
Entry | Database: PDB / ID: 8b7i | ||||||||||||
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Title | Human HSP90 alpha ATP Binding Domain, ATP-lid open conformation, R60A | ||||||||||||
Components | HSP90AA1 proteinHeat shock protein 90kDa alpha (cytosolic), member A1 | ||||||||||||
Keywords | CHAPERONE / Human Chaperone Protein / HSP90 alpha / N-Terminal Domain / ATP Binding Domain / Ground State / ATP-lid Open State | ||||||||||||
Function / homology | Function and homology information ATP-dependent protein folding chaperone / unfolded protein binding / melanosome / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | SOLUTION NMR / molecular dynamics | ||||||||||||
Authors | Rioual, E. / Henot, F. / Favier, A. / Macek, P. / Crublet, E. / Josso, P. / Brutscher, B. / Frech, M. / Gans, P. / Loison, C. / Boisbouvier, J. | ||||||||||||
Funding support | France, 3items
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Citation | Journal: Nat Commun / Year: 2022 Title: Visualizing the transiently populated closed-state of human HSP90 ATP binding domain. Authors: Henot, F. / Rioual, E. / Favier, A. / Macek, P. / Crublet, E. / Josso, P. / Brutscher, B. / Frech, M. / Gans, P. / Loison, C. / Boisbouvier, J. #1: Journal: Biorxiv / Year: 2022 Title: Visualizing the Transiently Populated Closed-State of Human HSP90 ATP Binding Domain Authors: Henot, F. / Rioual, E. / Favier, A. / Macek, P. / Crublet, E. / Josso, P. / Brutscher, B. / Frech, M. / Gans, P. / Loison, C. / Boisbouvier, J. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8b7i.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8b7i.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 8b7i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b7/8b7i ftp://data.pdbj.org/pub/pdb/validation_reports/b7/8b7i | HTTPS FTP |
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-Related structure data
Related structure data | 8b7jC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 29934.477 Da / Num. of mol.: 1 / Mutation: R60A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HSP90AA1 / Plasmid: pET-28 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: Q2VPJ6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 150 mM / Label: pH 7.5 / pH: 7.5 / Pressure: 1 bar / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 1000 / Conformers submitted total number: 20 |