+Open data
-Basic information
Entry | Database: PDB / ID: 7zy4 | ||||||
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Title | Crystal structure of human CstF77 in complex with hFip1 | ||||||
Components |
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Keywords | GENE REGULATION / Complex / 3' end processing / CstF / CPSF | ||||||
Function / homology | Function and homology information mRNA cleavage stimulating factor complex / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA 3'-end processing / Processing of Intronless Pre-mRNAs / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / Processing of Capped Intron-Containing Pre-mRNA / mRNA binding / RNA binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.55 Å | ||||||
Authors | Muckenfuss, L.M. / Jinek, M. | ||||||
Funding support | Switzerland, 1items
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Citation | Journal: Elife / Year: 2022 Title: Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3' end biogenesis. Authors: Muckenfuss, L.M. / Migenda Herranz, A.C. / Boneberg, F.M. / Clerici, M. / Jinek, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7zy4.cif.gz | 463.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7zy4.ent.gz | 323.9 KB | Display | PDB format |
PDBx/mmJSON format | 7zy4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zy/7zy4 ftp://data.pdbj.org/pub/pdb/validation_reports/zy/7zy4 | HTTPS FTP |
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-Related structure data
Related structure data | 7zyhC 2ooeS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: givenMatrix: (-0.982795530837, -0.18213725633, 0.0306425263796), (-0.184341994966, 0.957032937474, -0.223843663037), (0.0114443635997, -0.225641256083, -0.974143239003)Vector: 12. ...NCS oper: (Code: given Matrix: (-0.982795530837, -0.18213725633, 0.0306425263796), Vector: |
-Components
#1: Protein | Mass: 36598.852 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CSTF3 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12996 #2: Protein/peptide | Mass: 4133.248 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.89 Å3/Da / Density % sol: 68.4 % |
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Crystal grow | Temperature: 278.15 K / Method: vapor diffusion, sitting drop Details: 0.1 M Bicine pH 9.0, 10% w/v PEG 20k, 2% v/v Dioxane |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 21, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.55→56.31 Å / Num. obs: 38981 / % possible obs: 99.96 % / Redundancy: 40.5 % / Biso Wilson estimate: 70.93 Å2 / CC1/2: 1 / Net I/σ(I): 36 |
Reflection shell | Resolution: 2.55→2.641 Å / Redundancy: 42.3 % / Num. unique obs: 3836 / CC1/2: 0.836 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2OOE Resolution: 2.55→56.31 Å / SU ML: 0.4593 / Cross valid method: FREE R-VALUE / Phase error: 23.6984 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 70.37 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.55→56.31 Å
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Refine LS restraints |
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Refine LS restraints NCS | Type: Torsion NCS / Rms dev position: 1.62018133605 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 2.01762852962 Å / Origin y: 45.770957828 Å / Origin z: -0.0107682085167 Å
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Refinement TLS group | Selection details: all |