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- PDB-7t12: Hexameric HIV-1 (O-group) CA -

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Basic information

Entry
Database: PDB / ID: 7t12
TitleHexameric HIV-1 (O-group) CA
ComponentsCapsid protein p24
KeywordsVIRAL PROTEIN / Capsid
Function / homology
Function and homology information


viral budding via host ESCRT complex / host multivesicular body / viral nucleocapsid / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / RNA binding / zinc ion binding / membrane
Similarity search - Function
Retroviral nucleocapsid Gag protein p24, N-terminal / gag protein p24 N-terminal domain / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retrovirus capsid, C-terminal / Retroviral matrix protein / Retrovirus capsid, N-terminal ...Retroviral nucleocapsid Gag protein p24, N-terminal / gag protein p24 N-terminal domain / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retrovirus capsid, C-terminal / Retroviral matrix protein / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile.
Similarity search - Domain/homology
Biological speciesHIV-1 O_MVP5180 (virus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3 Å
AuthorsJacques, D.A. / James, L.C.
Funding support Australia, United Kingdom, European Union, 6items
OrganizationGrant numberCountry
Australian Research Council (ARC)DP180101384 Australia
National Health and Medical Research Council (NHMRC, Australia)GNT1158338 Australia
Wellcome Trust214344/Z/18/Z United Kingdom
Wellcome Trust108183 United Kingdom
Wellcome Trust220863 United Kingdom
European Research Council (ERC)339223European Union
CitationJournal: Nat Microbiol / Year: 2022
Title: Evasion of cGAS and TRIM5 defines pandemic HIV.
Authors: Zuliani-Alvarez, L. / Govasli, M.L. / Rasaiyaah, J. / Monit, C. / Perry, S.O. / Sumner, R.P. / McAlpine-Scott, S. / Dickson, C. / Rifat Faysal, K.M. / Hilditch, L. / Miles, R.J. / Bibollet- ...Authors: Zuliani-Alvarez, L. / Govasli, M.L. / Rasaiyaah, J. / Monit, C. / Perry, S.O. / Sumner, R.P. / McAlpine-Scott, S. / Dickson, C. / Rifat Faysal, K.M. / Hilditch, L. / Miles, R.J. / Bibollet-Ruche, F. / Hahn, B.H. / Boecking, T. / Pinotsis, N. / James, L.C. / Jacques, D.A. / Towers, G.J.
History
DepositionDec 1, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 19, 2022Provider: repository / Type: Initial release
Revision 1.1Nov 9, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Capsid protein p24
B: Capsid protein p24
C: Capsid protein p24
D: Capsid protein p24
E: Capsid protein p24
F: Capsid protein p24
G: Capsid protein p24
H: Capsid protein p24
I: Capsid protein p24
J: Capsid protein p24
K: Capsid protein p24
L: Capsid protein p24


Theoretical massNumber of molelcules
Total (without water)305,65612
Polymers305,65612
Non-polymers00
Water0
1
A: Capsid protein p24
B: Capsid protein p24
C: Capsid protein p24
D: Capsid protein p24
E: Capsid protein p24
F: Capsid protein p24


Theoretical massNumber of molelcules
Total (without water)152,8286
Polymers152,8286
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area13160 Å2
ΔGint-95 kcal/mol
Surface area58540 Å2
MethodPISA
2
G: Capsid protein p24
H: Capsid protein p24
I: Capsid protein p24
J: Capsid protein p24
K: Capsid protein p24
L: Capsid protein p24


Theoretical massNumber of molelcules
Total (without water)152,8286
Polymers152,8286
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area13320 Å2
ΔGint-101 kcal/mol
Surface area57300 Å2
MethodPISA
Unit cell
Length a, b, c (Å)87.200, 151.730, 120.420
Angle α, β, γ (deg.)90.000, 105.030, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 1 through 37 or resid 39...
21(chain B and (resid 1 through 37 or resid 39...
31(chain C and (resid 1 through 37 or resid 39...
41(chain D and (resid 1 through 37 or resid 39...
51(chain E and (resid 1 through 37 or resid 39...
61(chain F and (resid 1 through 37 or resid 39...
71(chain G and (resid 1 through 37 or resid 39...
81(chain H and (resid 1 through 37 or resid 39...
91(chain I and (resid 1 through 37 or resid 39...
101(chain J and (resid 1 through 37 or resid 39...
111(chain K and (resid 1 through 37 or resid 39...
121(chain L and (resid 1 through 37 or resid 39...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resid 1 through 37 or resid 39...A1 - 37
121(chain A and (resid 1 through 37 or resid 39...A39 - 85
131(chain A and (resid 1 through 37 or resid 39...A1 - 220
141(chain A and (resid 1 through 37 or resid 39...A1 - 220
151(chain A and (resid 1 through 37 or resid 39...A1
161(chain A and (resid 1 through 37 or resid 39...A1 - 220
171(chain A and (resid 1 through 37 or resid 39...A1 - 220
181(chain A and (resid 1 through 37 or resid 39...A1 - 220
191(chain A and (resid 1 through 37 or resid 39...A1 - 220
1101(chain A and (resid 1 through 37 or resid 39...A1 - 220
211(chain B and (resid 1 through 37 or resid 39...B1 - 37
221(chain B and (resid 1 through 37 or resid 39...B39 - 85
231(chain B and (resid 1 through 37 or resid 39...B1 - 220
241(chain B and (resid 1 through 37 or resid 39...B1 - 220
251(chain B and (resid 1 through 37 or resid 39...B12
261(chain B and (resid 1 through 37 or resid 39...B1 - 220
271(chain B and (resid 1 through 37 or resid 39...B1 - 220
281(chain B and (resid 1 through 37 or resid 39...B1 - 220
291(chain B and (resid 1 through 37 or resid 39...B1 - 220
311(chain C and (resid 1 through 37 or resid 39...C1 - 37
321(chain C and (resid 1 through 37 or resid 39...C39 - 85
331(chain C and (resid 1 through 37 or resid 39...C97 - 119
341(chain C and (resid 1 through 37 or resid 39...C120
351(chain C and (resid 1 through 37 or resid 39...C1 - 220
361(chain C and (resid 1 through 37 or resid 39...C1 - 220
371(chain C and (resid 1 through 37 or resid 39...C1 - 220
381(chain C and (resid 1 through 37 or resid 39...C1 - 220
411(chain D and (resid 1 through 37 or resid 39...D1 - 37
421(chain D and (resid 1 through 37 or resid 39...D39 - 85
431(chain D and (resid 1 through 37 or resid 39...D97 - 150
441(chain D and (resid 1 through 37 or resid 39...D1 - 220
451(chain D and (resid 1 through 37 or resid 39...D176 - 189
461(chain D and (resid 1 through 37 or resid 39...D1 - 220
471(chain D and (resid 1 through 37 or resid 39...D1 - 220
481(chain D and (resid 1 through 37 or resid 39...D1 - 220
491(chain D and (resid 1 through 37 or resid 39...D1 - 220
4101(chain D and (resid 1 through 37 or resid 39...D1 - 220
511(chain E and (resid 1 through 37 or resid 39...E1 - 37
521(chain E and (resid 1 through 37 or resid 39...E39 - 85
531(chain E and (resid 1 through 37 or resid 39...E97 - 119
541(chain E and (resid 1 through 37 or resid 39...E120
551(chain E and (resid 1 through 37 or resid 39...E1 - 220
561(chain E and (resid 1 through 37 or resid 39...E1 - 220
571(chain E and (resid 1 through 37 or resid 39...E1 - 220
581(chain E and (resid 1 through 37 or resid 39...E1 - 220
611(chain F and (resid 1 through 37 or resid 39...F1 - 37
621(chain F and (resid 1 through 37 or resid 39...F39 - 85
631(chain F and (resid 1 through 37 or resid 39...F97 - 119
641(chain F and (resid 1 through 37 or resid 39...F120
651(chain F and (resid 1 through 37 or resid 39...F1 - 220
661(chain F and (resid 1 through 37 or resid 39...F1 - 220
671(chain F and (resid 1 through 37 or resid 39...F1 - 220
681(chain F and (resid 1 through 37 or resid 39...F1 - 220
711(chain G and (resid 1 through 37 or resid 39...G1 - 37
721(chain G and (resid 1 through 37 or resid 39...G39 - 85
731(chain G and (resid 1 through 37 or resid 39...G1 - 220
741(chain G and (resid 1 through 37 or resid 39...G1 - 220
751(chain G and (resid 1 through 37 or resid 39...G120
761(chain G and (resid 1 through 37 or resid 39...G1 - 220
771(chain G and (resid 1 through 37 or resid 39...G1 - 220
781(chain G and (resid 1 through 37 or resid 39...G1 - 220
791(chain G and (resid 1 through 37 or resid 39...G1 - 220
811(chain H and (resid 1 through 37 or resid 39...H1 - 37
821(chain H and (resid 1 through 37 or resid 39...H39 - 85
831(chain H and (resid 1 through 37 or resid 39...H97 - 11
841(chain H and (resid 1 through 37 or resid 39...H1 - 220
851(chain H and (resid 1 through 37 or resid 39...H1 - 220
861(chain H and (resid 1 through 37 or resid 39...H1 - 220
871(chain H and (resid 1 through 37 or resid 39...H1 - 220
881(chain H and (resid 1 through 37 or resid 39...H1 - 220
911(chain I and (resid 1 through 37 or resid 39...I1 - 37
921(chain I and (resid 1 through 37 or resid 39...I39 - 85
931(chain I and (resid 1 through 37 or resid 39...I97 - 119
941(chain I and (resid 1 through 37 or resid 39...I120
951(chain I and (resid 1 through 37 or resid 39...I1 - 220
961(chain I and (resid 1 through 37 or resid 39...I1 - 220
971(chain I and (resid 1 through 37 or resid 39...I1 - 220
981(chain I and (resid 1 through 37 or resid 39...I1 - 220
1011(chain J and (resid 1 through 37 or resid 39...J1 - 37
1021(chain J and (resid 1 through 37 or resid 39...J39 - 85
1031(chain J and (resid 1 through 37 or resid 39...J1 - 220
1041(chain J and (resid 1 through 37 or resid 39...J1 - 220
1051(chain J and (resid 1 through 37 or resid 39...J152
1061(chain J and (resid 1 through 37 or resid 39...J159 - 175
1071(chain J and (resid 1 through 37 or resid 39...J0
1081(chain J and (resid 1 through 37 or resid 39...J1 - 220
1091(chain J and (resid 1 through 37 or resid 39...J1 - 220
10101(chain J and (resid 1 through 37 or resid 39...J1 - 220
10111(chain J and (resid 1 through 37 or resid 39...J1 - 220
1111(chain K and (resid 1 through 37 or resid 39...K1 - 37
1121(chain K and (resid 1 through 37 or resid 39...K39 - 150
1131(chain K and (resid 1 through 37 or resid 39...K152 - 57
1141(chain K and (resid 1 through 37 or resid 39...K1 - 220
1151(chain K and (resid 1 through 37 or resid 39...K1 - 220
1161(chain K and (resid 1 through 37 or resid 39...K1 - 220
1171(chain K and (resid 1 through 37 or resid 39...K1 - 220
1181(chain K and (resid 1 through 37 or resid 39...K1 - 220
1191(chain K and (resid 1 through 37 or resid 39...K1 - 220
1211(chain L and (resid 1 through 37 or resid 39...L1 - 37
1221(chain L and (resid 1 through 37 or resid 39...L39 - 85
1231(chain L and (resid 1 through 37 or resid 39...L97 - 119
1241(chain L and (resid 1 through 37 or resid 39...L120
1251(chain L and (resid 1 through 37 or resid 39...L1 - 220
1261(chain L and (resid 1 through 37 or resid 39...L1 - 220
1271(chain L and (resid 1 through 37 or resid 39...L1 - 220
1281(chain L and (resid 1 through 37 or resid 39...L1 - 220

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Components

#1: Protein
Capsid protein p24 / / CA


Mass: 25471.320 Da / Num. of mol.: 12 / Mutation: A14C, E45C, W185A, M186A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HIV-1 O_MVP5180 (virus) / Strain: isolate MVP5180 / Gene: gag / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): C41 / References: UniProt: Q79665

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.52 Å3/Da / Density % sol: 51.13 %
Crystal growTemperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7
Details: Crystallant: 10% (w/v) PEG 6000, 100 mM HEPES (pH 7.0), 100 mM Glycine. Crystals grew in 2 ul protein (40 mg/ml) + 2 ul crystallant. Cryoprotected in 40% (w/v) glucose

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.97945 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 2, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97945 Å / Relative weight: 1
ReflectionResolution: 3→75.86 Å / Num. obs: 59020 / % possible obs: 97.7 % / Redundancy: 5 % / CC1/2: 0.908 / Rmerge(I) obs: 0.17 / Rpim(I) all: 0.088 / Rrim(I) all: 0.193 / Net I/σ(I): 5.9 / Num. measured all: 295052 / Scaling rejects: 3631
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
3-3.084.71.2082130045800.2820.6261.3691.897.5
13.08-75.865.10.11438327520.9750.0560.1299.298

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation3 Å75.86 Å
Translation3 Å75.86 Å

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Processing

Software
NameVersionClassification
MOSFLMdata reduction
Aimless0.6.3data scaling
PHASER2.8.2phasing
PHENIX1.14refinement
PDB_EXTRACT3.27data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3H47

3h47


Resolution: 3→75.86 Å / SU ML: 0.5 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 28.25 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2586 2934 5.01 %
Rwork0.2144 55643 -
obs0.2166 58577 96.74 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 241.15 Å2 / Biso mean: 95.3919 Å2 / Biso min: 27.95 Å2
Refinement stepCycle: final / Resolution: 3→75.86 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18717 0 0 0 18717
Num. residues----2419
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6504X-RAY DIFFRACTION1.955TORSIONAL
12B6504X-RAY DIFFRACTION1.955TORSIONAL
13C6504X-RAY DIFFRACTION1.955TORSIONAL
14D6504X-RAY DIFFRACTION1.955TORSIONAL
15E6504X-RAY DIFFRACTION1.955TORSIONAL
16F6504X-RAY DIFFRACTION1.955TORSIONAL
17G6504X-RAY DIFFRACTION1.955TORSIONAL
18H6504X-RAY DIFFRACTION1.955TORSIONAL
19I6504X-RAY DIFFRACTION1.955TORSIONAL
110J6504X-RAY DIFFRACTION1.955TORSIONAL
111K6504X-RAY DIFFRACTION1.955TORSIONAL
112L6504X-RAY DIFFRACTION1.955TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3-3.04920.37281320.3118262898
3.0492-3.10180.30561560.3035264397
3.1018-3.15820.37461320.2714265898
3.1582-3.2190.3261560.2591271498
3.219-3.28470.31921460.2646262998
3.2847-3.35610.31121390.2609273498
3.3561-3.43420.44161240.3481260296
3.4342-3.520.43851320.3613259495
3.52-3.61520.30511240.2526268198
3.6152-3.72160.30931370.3274249692
3.7216-3.84170.29241590.2279262796
3.8417-3.9790.32591300.2996251491
3.979-4.13830.27231560.1815266598
4.1383-4.32660.21651370.1708265197
4.3266-4.55470.19851390.1545266898
4.5547-4.840.19941280.1473272698
4.84-5.21370.21981230.1598267697
5.2137-5.73820.23691520.1757269799
5.7382-6.56810.24591440.1951267597
6.5681-8.27350.22321350.1873270297
8.2735-75.860.2021530.1854266395
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.78630.34960.39345.9074-0.95788.4301-0.05830.5193-0.439-0.36660.00170.61070.85120.03760.050.3904-0.06990.02140.36660.00850.601711.178-8.867110.055
26.13480.3038-2.76534.983-0.14129.4669-0.0613-0.9711-0.07630.8392-0.1222-0.40770.15330.9793-0.19040.5117-0.0508-0.03390.627-0.03990.3228-2.562-6.348142.182
33.77260.6313-1.48945.2781-0.13937.217-0.10380.422-0.3018-0.0615-0.21470.18830.3675-0.41850.22190.2797-0.0137-0.02330.4305-0.0530.4215-12.149-10.65118.413
43.3629-3.2687-1.97927.97882.99546.92130.0193-0.6209-0.01260.4534-0.10450.88280.004-0.96730.1350.52160.17150.17480.9471-0.04450.6278-34.50812.823134.782
56.93361.1661.05436.92871.3576.99710.03580.30320.1921-0.1767-0.010.66920.2317-0.2319-00.3370.02490.01660.35910.07570.4426-29.7135.656109.57
61.0178-1.2676-0.75914.61431.75354.93770.27230.14431.29051.19090.35840.8776-1.592-0.0872-0.39730.9190.0870.40410.57560.10231.7277-27.80243.641114.375
75.45120.22112.55325.22490.11287.14540.27330.61580.3382-0.5621-0.02391.00270.2127-0.2378-0.14550.36860.0676-0.00680.57370.12310.7499-23.74225.78894.553
84.79070.75452.4379.81231.99685.0459-0.45980.0022-0.1066-0.02470.781-1.7901-0.88270.7522-0.19740.5589-0.14370.18120.5425-0.1240.79154.50649.81497.276
96.0702-0.25671.07685.34562.48947.59020.02770.2556-0.1089-0.60390.03750.1621-0.0212-0.2021-0.09680.45550.0010.06790.3518-0.01550.4314-0.31626.30184.763
107.98553.90824.70156.6060.23176.99460.3165-0.9925-0.73190.80780.0129-1.8791-0.21830.6107-0.15560.6779-0.2017-0.05770.6680.04831.293233.12727.287102.412
114.4966-1.15130.92766.2277-0.96875.36870.30220.376-0.0413-0.9848-0.6231-0.58960.78620.25960.33080.90740.06560.23220.34550.06020.76516.9158.33491.507
125.47091.4731-0.72681.74521.3815.32280.0081-0.96980.56680.28830.1009-0.2604-0.6892.011-0.10120.3873-0.1533-0.0871.1394-0.03290.935229.3970.672126.011
133.88862.89052.18129.1362-0.08275.30370.02060.05730.40060.78060.02150.2792-0.08120.62340.08980.44690.04650.06940.617-0.07980.3406-20.882-32.35584.57
146.5991-3.34780.9547.8925-0.97970.88230.23670.0306-2.6284-0.0588-0.10190.84380.42520.1508-0.09390.65480.05160.10160.5569-0.03581.0946-8.393-68.08679.615
155.77230.46180.68224.38560.81615.6046-0.5149-0.94050.42960.27220.21210.0605-0.09730.00750.31270.41260.183-0.12340.7412-0.12420.54191.951-44.67586.979
162.0612-2.2704-0.46996.77312.12744.9735-1.29321.5932-2.3831-0.51090.3308-1.16660.9296-0.33520.57460.78160.11110.40391.1224-0.38891.109123.928-61.70662.772
176.23751.12772.00276.0220.75278.4142-0.54120.4881.5140.03010.15470.457-0.16570.10360.26610.46280.0918-0.01820.60520.09270.587521.045-36.46572.64
183.7379-1.34242.17263.4153.67688.20130.1427-0.291-0.5099-1.2024-0.1247-0.04990.38911.02070.05611.21530.25040.24521.1330.22380.608223.4-35.67535.53
193.91260.78882.79838.9204-1.23248.2725-0.3220.1160.19150.41910.48150.5985-0.01240.0751-0.12650.34630.19620.08270.74260.10880.530717.31-18.6355.692
207.5480.9514.76691.03381.50867.0459-1.0751.39430.0972-0.5816-0.26210.4787-1.48520.49740.17070.93810.0089-0.5540.97440.21770.9393-5.128-13.93127.944
213.8255-1.6932-3.08835.22934.3078.1595-0.1539-0.8703-0.4848-0.0714-0.16080.3986-0.81780.8050.17630.6254-0.109-0.19410.79280.24140.7778-5.527-8.20453.61
228.07793.45920.03775.575-0.28592.8571-0.26930.22660.3504-0.5649-0.3430.9348-0.2036-1.0290.54920.62490.1535-0.25141.0512-0.33980.7843-39.061-21.60145.628
234.92892.24452.58415.88480.33597.1108-0.5543-0.55230.53190.4525-0.6124-0.0537-1.3355-0.49350.8090.68680.1644-0.34220.888-0.1540.6787-25.088-15.61867.403
242.00121.8711-1.27618.5146-2.29962.5876-0.1561-0.1928-0.4165-1.39890.43020.86732.1386-2.4659-0.30581.2339-0.7138-0.03431.64680.14960.6658-39.241-48.95372.578
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 1:143 )A1 - 143
2X-RAY DIFFRACTION2( CHAIN A AND RESID 144:220 )A144 - 220
3X-RAY DIFFRACTION3( CHAIN B AND RESID 1:143 )B1 - 143
4X-RAY DIFFRACTION4( CHAIN B AND RESID 144:220 )B144 - 220
5X-RAY DIFFRACTION5( CHAIN C AND RESID 1:143 )C1 - 143
6X-RAY DIFFRACTION6( CHAIN C AND RESID 144:220 )C144 - 220
7X-RAY DIFFRACTION7( CHAIN D AND RESID 1:143 )D1 - 143
8X-RAY DIFFRACTION8( CHAIN D AND RESID 144:220 )D144 - 220
9X-RAY DIFFRACTION9( CHAIN E AND RESID 1:143 )E1 - 143
10X-RAY DIFFRACTION10( CHAIN E AND RESID 144:220 )E144 - 220
11X-RAY DIFFRACTION11( CHAIN F AND RESID 1:143 )F1 - 143
12X-RAY DIFFRACTION12( CHAIN F AND RESID 144:220 )F144 - 220
13X-RAY DIFFRACTION13( CHAIN G AND RESID 1:143 )G1 - 143
14X-RAY DIFFRACTION14( CHAIN G AND RESID 144:220 )G144 - 220
15X-RAY DIFFRACTION15( CHAIN H AND RESID 1:143 )H1 - 143
16X-RAY DIFFRACTION16( CHAIN H AND RESID 144:220 )H144 - 220
17X-RAY DIFFRACTION17( CHAIN I AND RESID 1:143 )I1 - 143
18X-RAY DIFFRACTION18( CHAIN I AND RESID 144:220 )I144 - 220
19X-RAY DIFFRACTION19( CHAIN J AND RESID 1:143 )J1 - 143
20X-RAY DIFFRACTION20( CHAIN J AND RESID 144:220 )J144 - 220
21X-RAY DIFFRACTION21( CHAIN K AND RESID 1:143 )K1 - 143
22X-RAY DIFFRACTION22( CHAIN K AND RESID 144:220 )K144 - 220
23X-RAY DIFFRACTION23( CHAIN L AND RESID 1:143 )L1 - 143
24X-RAY DIFFRACTION24( CHAIN L AND RESID 144:220 )L144 - 220

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