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- PDB-7lue: Prefusion RSV F glycoprotein bound by neutralizing site V-directe... -

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Basic information

Entry
Database: PDB / ID: 7lue
TitlePrefusion RSV F glycoprotein bound by neutralizing site V-directed antibody ADI-14442
Components
  • Fusion glycoprotein F0
  • Heavy chain of human antibody Fab ADI-14442
  • Light chain of human antibody Fab ADI-14442
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / fusion / immunoglobulin / public clonotype / convergent recognition / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


positive regulation of syncytium formation by virus / host cell Golgi membrane / entry receptor-mediated virion attachment to host cell / fusion of virus membrane with host plasma membrane / host cell plasma membrane / virion membrane / plasma membrane
Similarity search - Function
Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0
Similarity search - Domain/homology
Fusion glycoprotein F0
Similarity search - Component
Biological speciesRespiratory syncytial virus A2
Homo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsGilman, M.S.A. / McLellan, J.S.
CitationJournal: Immunity / Year: 2021
Title: Vaccination with prefusion-stabilized respiratory syncytial virus fusion protein induces genetically and antigenically diverse antibody responses.
Authors: Maryam Mukhamedova / Daniel Wrapp / Chen-Hsiang Shen / Morgan S A Gilman / Tracy J Ruckwardt / Chaim A Schramm / Larissa Ault / Lauren Chang / Alexandrine Derrien-Colemyn / Sarah A M Lucas / ...Authors: Maryam Mukhamedova / Daniel Wrapp / Chen-Hsiang Shen / Morgan S A Gilman / Tracy J Ruckwardt / Chaim A Schramm / Larissa Ault / Lauren Chang / Alexandrine Derrien-Colemyn / Sarah A M Lucas / Amy Ransier / Samuel Darko / Emily Phung / Lingshu Wang / Yi Zhang / Scott A Rush / Bharat Madan / Guillaume B E Stewart-Jones / Pamela J Costner / LaSonji A Holman / Somia P Hickman / Nina M Berkowitz / Nicole A Doria-Rose / Kaitlyn M Morabito / Brandon J DeKosky / Martin R Gaudinski / Grace L Chen / Michelle C Crank / John Misasi / Nancy J Sullivan / Daniel C Douek / Peter D Kwong / Barney S Graham / Jason S McLellan / John R Mascola /
Abstract: An effective vaccine for respiratory syncytial virus (RSV) is an unrealized public health goal. A single dose of the prefusion-stabilized fusion (F) glycoprotein subunit vaccine (DS-Cav1) ...An effective vaccine for respiratory syncytial virus (RSV) is an unrealized public health goal. A single dose of the prefusion-stabilized fusion (F) glycoprotein subunit vaccine (DS-Cav1) substantially increases serum-neutralizing activity in healthy adults. We sought to determine whether DS-Cav1 vaccination induces a repertoire mirroring the pre-existing diversity from natural infection or whether antibody lineages targeting specific epitopes predominate. We evaluated RSV F-specific B cell responses before and after vaccination in six participants using complementary B cell sequencing methodologies and identified 555 clonal lineages. DS-Cav1-induced lineages recognized the prefusion conformation of F (pre-F) and were genetically diverse. Expressed antibodies recognized all six antigenic sites on the pre-F trimer. We identified 34 public clonotypes, and structural analysis of two antibodies from a predominant clonotype revealed a common mode of recognition. Thus, vaccination with DS-Cav1 generates a diverse polyclonal response targeting the antigenic sites on pre-F, supporting the development and advanced testing of pre-F-based vaccines against RSV.
History
DepositionFeb 22, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 16, 2021Provider: repository / Type: Initial release

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Assembly

Deposited unit
A: Fusion glycoprotein F0
B: Fusion glycoprotein F0
C: Fusion glycoprotein F0
H: Heavy chain of human antibody Fab ADI-14442
I: Heavy chain of human antibody Fab ADI-14442
J: Heavy chain of human antibody Fab ADI-14442
L: Light chain of human antibody Fab ADI-14442
M: Light chain of human antibody Fab ADI-14442
N: Light chain of human antibody Fab ADI-14442


Theoretical massNumber of molelcules
Total (without water)327,1179
Polymers327,1179
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Fusion glycoprotein F0 / Fusion glycoprotein F1 / Fusion glycoprotein F2


Mass: 61132.672 Da / Num. of mol.: 3 / Mutation: N67I, S215P
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Respiratory syncytial virus A2 / Cell line (production host): FreeStyle 293-F / Production host: Homo sapiens (human) / References: UniProt: W8RJF9
#2: Antibody Heavy chain of human antibody Fab ADI-14442


Mass: 23805.615 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): FreeStyle 293-F / Production host: Homo sapiens (human)
#3: Antibody Light chain of human antibody Fab ADI-14442


Mass: 24100.822 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): FreeStyle 293-F / Production host: Homo sapiens (human)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeDetailsEntity IDParent-IDSource
1Trimeric prefusion RSV F glycoprotein bound by three molecules of Fab ADI-14442.COMPLEXSingle molecule of trimeric respiratory syncytial virus fusion protein ectodomain (RSV F) stabilized in the prefusion conformation and fused to the T4 fibritin (foldon) trimerization motif is bound by three molecules of Fab ADI-14442.all0RECOMBINANT
2Trimeric prefusion RSV F glycoproteinCOMPLEXSingle molecule of trimeric respiratory syncytial virus fusion protein ectodomain (RSV F) stabilized in the prefusion conformation and fused to the T4 fibritin (foldon) trimerization motif.#11RECOMBINANT
3Fab ADI-14442COMPLEXThree molecules of Fab ADI-14442, each composed of a heavy and a light chain, are bound to a single molecule of trimeric respiratory syncytial virus fusion protein ectodomain (RSV F) stabilized in the prefusion conformation and fused to the T4 fibritin (foldon) trimerization motif.#2-#31RECOMBINANT
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
11Respiratory syncytial virus A21972429
32Respiratory syncytial virus A21972429
43Homo sapiens (human)9606
21Homo sapiens (human)9606
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-IDCell
11Homo sapiens (human)9606FreeStyle 293-F
22Homo sapiens (human)9606FreeStyle 293-F
33Homo sapiens (human)9606FreeStyle 293-F
Buffer solutionpH: 8
Buffer component
IDConc.NameFormulaBuffer-ID
1200 mMSodium chlorideNaClSodium chloride1
22 mMTRIS hydrochlorideNH2C(CH2OH)3HCl1
30.02 %Sodium azideNaN31
SpecimenConc.: 0.8 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid type: C-flat-1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 22500 X / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm
Image recordingAverage exposure time: 9 sec. / Electron dose: 48 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)
Image scansMovie frames/image: 30

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Processing

EM software
IDNameVersionCategoryDetails
4CTFFIND4.1.10CTF correction
5RELION3CTF correctionbeta
11RELION3initial Euler assignmentbeta
12RELION3final Euler assignmentbeta
14RELION33D reconstructionbeta
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C3 (3 fold cyclic)
3D reconstructionResolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 371323 / Symmetry type: POINT

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