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- PDB-7e1v: Cryo-EM structure of apo hybrid respiratory supercomplex consisti... -

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Basic information

Entry
Database: PDB / ID: 7e1v
TitleCryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV
Components
  • (Cytochrome bc1 complex cytochrome ...) x 2
  • (Cytochrome c oxidase subunit ...) x 4
  • Cytochrome bc1 complex Rieske iron-sulfur subunit
  • Cytochrome c oxidase polypeptide 4
  • Prokaryotic respiratory supercomplex associate factor 1 PRSAF1
  • Uncharacterized protein MSMEG_4692/MSMEI_4575
KeywordsOXIDOREDUCTASE / Mycobacterium smegmatis / mycobacterium tuberculosis / complexIII / complexIV / electron transport / anti-TB drugs
Function / homology
Function and homology information


aerobic electron transport chain / cytochrome-c oxidase / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen / respirasome / respiratory electron transport chain / peptidoglycan-based cell wall / monooxygenase activity ...aerobic electron transport chain / cytochrome-c oxidase / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen / respirasome / respiratory electron transport chain / peptidoglycan-based cell wall / monooxygenase activity / 2 iron, 2 sulfur cluster binding / oxidoreductase activity / iron ion binding / copper ion binding / heme binding / membrane / metal ion binding / plasma membrane
Similarity search - Function
Protein of unknown function DUF5130 / Domain of unknown function (DUF5130) / QcrA subunit, N-terminal / QcrA subunit N-terminal region / Cytochrome bc1 complex, cytochrome c subunit / Cytochrome c oxidase subunit IV, actinobacteria / Cytochrome c oxidase subunit IV / Cytochrome b(N-terminal)/b6/petB / Cytochrome C oxidase, cbb3-type, subunit III / Cytochrome c oxidase subunit III ...Protein of unknown function DUF5130 / Domain of unknown function (DUF5130) / QcrA subunit, N-terminal / QcrA subunit N-terminal region / Cytochrome bc1 complex, cytochrome c subunit / Cytochrome c oxidase subunit IV, actinobacteria / Cytochrome c oxidase subunit IV / Cytochrome b(N-terminal)/b6/petB / Cytochrome C oxidase, cbb3-type, subunit III / Cytochrome c oxidase subunit III / Cytochrome c oxidase subunit III-like / Cytochrome c oxidase, subunit III, 4-helical bundle / Cytochrome c oxidase subunit III / Heme-copper oxidase subunit III family profile. / Cytochrome c oxidase subunit III-like superfamily / Cytochrome c/quinol oxidase subunit II / Copper centre Cu(A) / CO II and nitrous oxide reductase dinuclear copper centers signature. / Cytochrome C oxidase subunit II, transmembrane domain superfamily / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / Di-haem cytochrome, transmembrane / Cytochrome C oxidase subunit II, periplasmic domain / Rieske iron-sulphur protein / Cytochrome c oxidase subunit II-like C-terminal / Cytochrome oxidase subunit II copper A binding domain profile. / Cytochrome c / Rieske [2Fe-2S] iron-sulphur domain / Rieske [2Fe-2S] domain / Rieske [2Fe-2S] iron-sulfur domain profile. / Rieske [2Fe-2S] iron-sulphur domain superfamily / Cytochrome c family profile. / Cytochrome c-like domain / Cytochrome c-like domain superfamily / Cupredoxin / Prokaryotic membrane lipoprotein lipid attachment site profile.
Similarity search - Domain/homology
Chem-9Y0 / Chem-9YF / CARDIOLIPIN / COPPER (II) ION / FE2/S2 (INORGANIC) CLUSTER / HEME-A / HEME C / PROTOPORPHYRIN IX CONTAINING FE / MENAQUINONE-9 / PALMITIC ACID ...Chem-9Y0 / Chem-9YF / CARDIOLIPIN / COPPER (II) ION / FE2/S2 (INORGANIC) CLUSTER / HEME-A / HEME C / PROTOPORPHYRIN IX CONTAINING FE / MENAQUINONE-9 / PALMITIC ACID / Probable cytochrome c oxidase subunit 3 / Cytochrome c oxidase polypeptide 4 / cytochrome-c oxidase / Uncharacterized protein MSMEG_4692/MSMEI_4575 / Uncharacterized protein / Cytochrome bc1 complex Rieske iron-sulfur subunit / Cytochrome bc1 complex cytochrome c subunit / Cytochrome bc1 complex cytochrome b subunit
Similarity search - Component
Biological speciesMycobacterium tuberculosis H37Rv (bacteria)
Mycobacterium smegmatis MC2 51 (bacteria)
Mycolicibacterium smegmatis MC2 51 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.68 Å
AuthorsZhou, S. / Wang, W. / Gao, Y. / Gong, H. / Rao, Z.
Funding support China, 5items
OrganizationGrant numberCountry
Chinese Academy of SciencesXDB37030201 China
Chinese Academy of SciencesXDB37020203 China
Chinese Academy of Sciences2017YFC0840300 China
National Natural Science Foundation of China (NSFC)81520108019 China
National Natural Science Foundation of China (NSFC)813300237 China
CitationJournal: Elife / Year: 2021
Title: Structure of cytochrome in complex with Q203 and TB47, two anti-TB drug candidates.
Authors: Shan Zhou / Weiwei Wang / Xiaoting Zhou / Yuying Zhang / Yuezheng Lai / Yanting Tang / Jinxu Xu / Dongmei Li / Jianping Lin / Xiaolin Yang / Ting Ran / Hongming Chen / Luke W Guddat / Quan ...Authors: Shan Zhou / Weiwei Wang / Xiaoting Zhou / Yuying Zhang / Yuezheng Lai / Yanting Tang / Jinxu Xu / Dongmei Li / Jianping Lin / Xiaolin Yang / Ting Ran / Hongming Chen / Luke W Guddat / Quan Wang / Yan Gao / Zihe Rao / Hongri Gong /
Abstract: Pathogenic mycobacteria pose a sustained threat to global human health. Recently, cytochrome complexes have gained interest as targets for antibiotic drug development. However, there is currently no ...Pathogenic mycobacteria pose a sustained threat to global human health. Recently, cytochrome complexes have gained interest as targets for antibiotic drug development. However, there is currently no structural information for the cytochrome complex from these pathogenic mycobacteria. Here, we report the structures of cytochrome alone (2.68 Å resolution) and in complex with clinical drug candidates Q203 (2.67 Å resolution) and TB47 (2.93 Å resolution) determined by single-particle cryo-electron microscopy. cytochrome forms a dimeric assembly with endogenous menaquinone/menaquinol bound at the quinone/quinol-binding pockets. We observe Q203 and TB47 bound at the quinol-binding site and stabilized by hydrogen bonds with the side chains of Thr and Glu, residues that are conserved across pathogenic mycobacteria. These high-resolution images provide a basis for the design of new mycobacterial cytochrome inhibitors that could be developed into broad-spectrum drugs to treat mycobacterial infections.
History
DepositionFeb 3, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Oct 13, 2021Provider: repository / Type: Initial release
Revision 1.1Jul 20, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Jul 27, 2022Group: Database references / Category: citation / citation_author / Item: _citation.pdbx_database_id_PubMed / _citation.title

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Structure visualization

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Assembly

Deposited unit
E: Cytochrome c oxidase subunit 2
F: Cytochrome c oxidase subunit 1
G: Cytochrome c oxidase subunit 3
H: Cytochrome c oxidase polypeptide 4
I: Cytochrome c oxidase subunit CtaJ
J: Uncharacterized protein MSMEG_4692/MSMEI_4575
D: Prokaryotic respiratory supercomplex associate factor 1 PRSAF1
Q: Cytochrome c oxidase subunit 2
R: Cytochrome c oxidase subunit 1
S: Cytochrome c oxidase subunit 3
T: Cytochrome c oxidase polypeptide 4
U: Cytochrome c oxidase subunit CtaJ
V: Uncharacterized protein MSMEG_4692/MSMEI_4575
P: Prokaryotic respiratory supercomplex associate factor 1 PRSAF1
N: Cytochrome bc1 complex cytochrome b subunit
M: Cytochrome bc1 complex Rieske iron-sulfur subunit
O: Cytochrome bc1 complex cytochrome c subunit
B: Cytochrome bc1 complex cytochrome b subunit
A: Cytochrome bc1 complex Rieske iron-sulfur subunit
C: Cytochrome bc1 complex cytochrome c subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)672,68179
Polymers624,85420
Non-polymers47,82759
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area198770 Å2
ΔGint-1808 kcal/mol
Surface area175130 Å2

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Components

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Cytochrome c oxidase subunit ... , 4 types, 8 molecules EQFRGSIU

#1: Protein Cytochrome c oxidase subunit 2 / / Cytochrome C oxidase subunit II ctaC


Mass: 38077.465 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium smegmatis MC2 51 (bacteria) / References: UniProt: A0R057, cytochrome-c oxidase
#2: Protein Cytochrome c oxidase subunit 1 /


Mass: 64162.965 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Mycolicibacterium smegmatis MC2 51 (bacteria)
#3: Protein Cytochrome c oxidase subunit 3 / / Cytochrome c oxidase subunit 3 CtaE


Mass: 22196.883 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium smegmatis MC2 51 (bacteria) / References: UniProt: A0R049
#5: Protein Cytochrome c oxidase subunit CtaJ /


Mass: 8365.549 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium smegmatis MC2 51 (bacteria) / References: UniProt: A0R1B6

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Protein , 4 types, 8 molecules HTJVDPMA

#4: Protein Cytochrome c oxidase polypeptide 4 / Cytochrome aa3 subunit 4 / Cytochrome c oxidase polypeptide IV


Mass: 15177.424 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium smegmatis MC2 51 (bacteria) / References: UniProt: A0R056, cytochrome-c oxidase
#6: Protein Uncharacterized protein MSMEG_4692/MSMEI_4575 / Cytochrome c oxidase subunit CtaI


Mass: 15910.971 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium smegmatis MC2 51 (bacteria) / References: UniProt: A0R1B5
#7: Protein Prokaryotic respiratory supercomplex associate factor 1 PRSAF1


Mass: 11329.909 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Mycolicibacterium smegmatis MC2 51 (bacteria)
#9: Protein Cytochrome bc1 complex Rieske iron-sulfur subunit / Cytochrome bc1 reductase complex subunit QcrA / Rieske iron-sulfur protein / Ubiquinol--cytochrome ...Cytochrome bc1 reductase complex subunit QcrA / Rieske iron-sulfur protein / Ubiquinol--cytochrome c reductase iron-sulfur subunit


Mass: 46976.465 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: qcrA, Rv2195, MTCY190.06
Production host: Mycolicibacterium smegmatis MC2 155 (bacteria)
References: UniProt: P9WH23

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Cytochrome bc1 complex cytochrome ... , 2 types, 4 molecules NBOC

#8: Protein Cytochrome bc1 complex cytochrome b subunit / Cytochrome bc1 reductase complex subunit QcrB / Ubiquinol--cytochrome c reductase cytochrome b subunit


Mass: 61077.062 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: qcrB, Rv2196, MTCY190.07
Production host: Mycolicibacterium smegmatis MC2 155 (bacteria)
References: UniProt: P9WP37, quinol-cytochrome-c reductase
#10: Protein Cytochrome bc1 complex cytochrome c subunit / Cytochrome bc1 reductase complex subunit QcrC / Ubiquinol--cytochrome c reductase cytochrome c subunit


Mass: 29152.365 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: qcrC, Rv2194, MTCY190.05
Production host: Mycolicibacterium smegmatis MC2 155 (bacteria)
References: UniProt: P9WP35, quinol-cytochrome-c reductase

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Non-polymers , 10 types, 59 molecules

#11: Chemical
ChemComp-CU / COPPER (II) ION / Copper


Mass: 63.546 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Cu
#12: Chemical
ChemComp-CDL / CARDIOLIPIN / DIPHOSPHATIDYL GLYCEROL / BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL / Cardiolipin


Mass: 1464.043 Da / Num. of mol.: 17 / Source method: obtained synthetically / Formula: C81H156O17P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: phospholipid*YM
#13: Chemical
ChemComp-PLM / PALMITIC ACID / Palmitic acid


Mass: 256.424 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C16H32O2 / Feature type: SUBJECT OF INVESTIGATION
#14: Chemical
ChemComp-HEA / HEME-A / Heme A


Mass: 852.837 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C49H56FeN4O6 / Feature type: SUBJECT OF INVESTIGATION
#15: Chemical ChemComp-9Y0 / (2R)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (E)-octadec-9-enoate


Mass: 717.996 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C39H76NO8P / Feature type: SUBJECT OF INVESTIGATION
#16: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME / Heme B


Mass: 616.487 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C34H32FeN4O4 / Feature type: SUBJECT OF INVESTIGATION
#17: Chemical
ChemComp-MQ9 / MENAQUINONE-9 / Vitamin K2


Mass: 785.233 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C56H80O2 / Feature type: SUBJECT OF INVESTIGATION
#18: Chemical ChemComp-FES / FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster


Mass: 175.820 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe2S2
#19: Chemical
ChemComp-9YF / (2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate


Mass: 853.112 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C44H85O13P
#20: Chemical
ChemComp-HEC / HEME C / Heme C


Mass: 618.503 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C34H34FeN4O4 / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeEntity IDParent-IDSource
1apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIVCOMPLEX#1-#100MULTIPLE SOURCES
2Mycobacterium tuberculosis complexIIICOMPLEX#8-#101RECOMBINANT
3Mycobacterium smegmatis complexIVCOMPLEX#1-#71NATURAL
Molecular weightValue: 0.8 MDa / Experimental value: YES
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
11Mycolicibacterium smegmatis MC2 51 (bacteria)1445611
22Mycobacterium tuberculosis H37Rv (bacteria)83332
Source (recombinant)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Buffer solutionpH: 7.4
SpecimenConc.: 10 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Calibrated defocus min: 1200 nm / Calibrated defocus max: 1800 nm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.16_3549: / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.68 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 112804 / Symmetry type: POINT
RefinementHighest resolution: 2.68 Å
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00443590
ELECTRON MICROSCOPYf_angle_d0.6959216
ELECTRON MICROSCOPYf_dihedral_angle_d12.66424565
ELECTRON MICROSCOPYf_chiral_restr0.0456239
ELECTRON MICROSCOPYf_plane_restr0.0067173

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