+Open data
-Basic information
Entry | Database: PDB / ID: 7cun | ||||||
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Title | The structure of human Integrator-PP2A complex | ||||||
Components |
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Keywords | TRANSCRIPTION / Integrator-PP2A complex / phosphatase | ||||||
Function / homology | Function and homology information U2 snRNA 3'-end processing / snRNA processing / integrator complex / intercellular transport / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / MASTL Facilitates Mitotic Progression ...U2 snRNA 3'-end processing / snRNA processing / integrator complex / intercellular transport / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / MASTL Facilitates Mitotic Progression / mitotic sister chromatid separation / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation / : / regulation of transcription elongation by RNA polymerase II / peptidyl-threonine dephosphorylation / negative regulation of tyrosine phosphorylation of STAT protein / positive regulation of microtubule binding / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein antigen binding / ceramide metabolic process / protein phosphatase regulator activity / GABA receptor binding / negative regulation of epithelial to mesenchymal transition / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / Initiation of Nuclear Envelope (NE) Reformation / ERKs are inactivated / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / regulation of Wnt signaling pathway / regulation of growth / Disassembly of the destruction complex and recruitment of AXIN to the membrane / inner cell mass cell proliferation / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / protein serine/threonine phosphatase activity / CTLA4 inhibitory signaling / Platelet sensitization by LDL / negative regulation of MAPK cascade / protein-serine/threonine phosphatase / regulation of cell differentiation / T cell homeostasis / ERK/MAPK targets / regulation of G1/S transition of mitotic cell cycle / phosphoprotein phosphatase activity / regulation of DNA replication / mesoderm development / RNA polymerase II transcribes snRNA genes / chromosome, centromeric region / DARPP-32 events / lateral plasma membrane / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / regulation of cell adhesion / Cyclin A/B1/B2 associated events during G2/M transition / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / RNA endonuclease activity / embryo implantation / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Resolution of Sister Chromatid Cohesion / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / protein dephosphorylation / RNA splicing / meiotic cell cycle / DNA damage checkpoint signaling / response to organic substance / protein tyrosine phosphatase activity / chromosome segregation / cellular response to ionizing radiation / RHO GTPases Activate Formins / response to lead ion / regulation of protein phosphorylation / negative regulation of transforming growth factor beta receptor signaling pathway / Spry regulation of FGF signaling / RAF activation / Degradation of beta-catenin by the destruction complex / PKR-mediated signaling / tau protein binding / positive regulation of protein serine/threonine kinase activity / negative regulation of cell growth / spindle pole Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å | ||||||
Authors | Zheng, H. / Qi, Y. / Liu, W. / Li, J. / Wang, J. / Xu, Y. | ||||||
Citation | Journal: Science / Year: 2020 Title: Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. Authors: Hai Zheng / Yilun Qi / Shibin Hu / Xuan Cao / Congling Xu / Zhinang Yin / Xizi Chen / Yan Li / Weida Liu / Jie Li / Jiawei Wang / Gang Wei / Kaiwei Liang / Fei Xavier Chen / Yanhui Xu / Abstract: The 14-subunit metazoan-specific Integrator contains an endonuclease that cleaves nascent RNA transcripts. Here, we identified a complex containing Integrator and protein phosphatase 2A core enzyme ...The 14-subunit metazoan-specific Integrator contains an endonuclease that cleaves nascent RNA transcripts. Here, we identified a complex containing Integrator and protein phosphatase 2A core enzyme (PP2A-AC), termed INTAC. The 3.5-angstrom-resolution structure reveals that nine human Integrator subunits and PP2A-AC assemble into a cruciform-shaped central scaffold formed by the backbone and shoulder modules, with the phosphatase and endonuclease modules flanking the opposite sides. As a noncanonical PP2A holoenzyme, the INTAC complex dephosphorylates the carboxy-terminal repeat domain of RNA polymerase II at serine-2, -5, and -7 and thus regulates transcription. Our study extends the function of PP2A to transcriptional regulation and reveals how dual enzymatic activities-RNA cleavage and RNA polymerase II dephosphorylation-are structurally and functionally integrated into the INTAC complex. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7cun.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7cun.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 7cun.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cu/7cun ftp://data.pdbj.org/pub/pdb/validation_reports/cu/7cun | HTTPS FTP |
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-Related structure data
Related structure data | 30473MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Integrator complex subunit ... , 9 types, 9 molecules ABDEFGHIK
#1: Protein | Mass: 244574.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS1, KIAA1440, UNQ1821/PRO3434 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q8N201 |
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#2: Protein | Mass: 134451.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS2, KIAA1287 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q9H0H0 |
#3: Protein | Mass: 108306.758 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS4, MSTP093 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q96HW7 |
#4: Protein | Mass: 108115.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS5, KIAA1698 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q6P9B9 |
#5: Protein | Mass: 100527.078 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS6, DBI1, DDX26, DDX26A / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q9UL03 |
#6: Protein | Mass: 106952.617 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS7, C1orf73 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q9NVH2 |
#7: Protein | Mass: 113219.859 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS8, C8orf52 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q75QN2 |
#8: Protein | Mass: 73891.219 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS9, RC74 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q9NV88 |
#9: Protein | Mass: 67756.562 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INTS11, CPSF3L, RC68 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) References: UniProt: Q5TA45, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters |
-Protein , 3 types, 3 molecules PQU
#10: Protein | Mass: 66034.883 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P30153 |
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#11: Protein | Mass: 35636.152 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) References: UniProt: P67775, protein-serine/threonine phosphatase |
#12: Protein | Mass: 50570.449 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 / Production host: Homo sapiens (human) |
-Non-polymers , 2 types, 4 molecules
#13: Chemical | #14: Chemical | |
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-Details
Has ligand of interest | N |
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Sequence details | For chain U, the density of UNK residues is too cracked or discontinuous to assign fitable sequence. |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
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Source (recombinant) |
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Buffer solution | pH: 7.4 | ||||||||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.16_3549: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 68378 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.99 Å2 | ||||||||||||||||||||||||
Refine LS restraints |
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