+Open data
-Basic information
Entry | Database: PDB / ID: 7cip | ||||||
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Title | Microbial Hormone-sensitive lipase E53 wild type | ||||||
Components | Lipase | ||||||
Keywords | HYDROLASE / Esterase / Microbial Hormone-sensitive lipase | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Erythrobacter longus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.752 Å | ||||||
Authors | Yang, X. / Li, Z. / Xu, X. / Li, J. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Microbial Hormone-sensitive lipase E53 wild type Authors: Yang, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7cip.cif.gz | 284.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7cip.ent.gz | 223.4 KB | Display | PDB format |
PDBx/mmJSON format | 7cip.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ci/7cip ftp://data.pdbj.org/pub/pdb/validation_reports/ci/7cip | HTTPS FTP |
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-Related structure data
Related structure data | 4ypvS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 33158.730 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Erythrobacter longus (bacteria) / Gene: EH31_02760 / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): K-12 / References: UniProt: A0A074MDU6 |
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-Non-polymers , 12 types, 1521 molecules
#2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-6NA / #5: Chemical | ChemComp-DMS / #6: Chemical | ChemComp-CCN / #7: Chemical | ChemComp-D8F / ( #8: Chemical | #9: Chemical | ChemComp-DIO / | #10: Chemical | ChemComp-BUA / | #11: Chemical | #12: Chemical | ChemComp-NA / | #13: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.82 Å3/Da / Density % sol: 67.79 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop Details: MES pH 6.5, PEG, ammonium sulfate, dioxane, PEG 1000 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97915 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 24, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 1.752→48.71 Å / Num. obs: 203688 / % possible obs: 99.86 % / Redundancy: 2 % / Biso Wilson estimate: 26.03 Å2 / CC1/2: 0.998 / CC star: 1 / Net I/σ(I): 14.19 |
Reflection shell | Resolution: 1.752→1.815 Å / Num. unique obs: 20074 / CC1/2: 0.849 / CC star: 0.958 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4YPV Resolution: 1.752→48.71 Å / Cross valid method: FREE R-VALUE Details: Hydrogens have been added in their riding positions
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Displacement parameters | Biso mean: 31.71 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.752→48.71 Å
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