+Open data
-Basic information
Entry | Database: PDB / ID: 6snl | ||||||
---|---|---|---|---|---|---|---|
Title | (R)-selective amine transaminase from Exophiala sideris | ||||||
Components | amine transaminase | ||||||
Keywords | TRANSFERASE / fold IV PLP-dependent enzyme / amine transaminase | ||||||
Function / homology | Aminotransferase class IV / Aminotransferase-like, PLP-dependent enzymes / Branched-chain-amino-acid aminotransferase-like, N-terminal / Branched-chain-amino-acid aminotransferase-like, C-terminal / Amino-transferase class IV / catalytic activity / PYRIDOXAL-5'-PHOSPHATE / Uncharacterized protein Function and homology information | ||||||
Biological species | Exophiala sideris (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.129 Å | ||||||
Authors | Telzerow, A. / Hakansson, M. / Steiner, K. | ||||||
Funding support | Austria, 1items
| ||||||
Citation | Journal: Chembiochem / Year: 2021 Title: Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members. Authors: Telzerow, A. / Paris, J. / Hakansson, M. / Gonzalez-Sabin, J. / Rios-Lombardia, N. / Groger, H. / Moris, F. / Schurmann, M. / Schwab, H. / Steiner, K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6snl.cif.gz | 369 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6snl.ent.gz | 300.9 KB | Display | PDB format |
PDBx/mmJSON format | 6snl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sn/6snl ftp://data.pdbj.org/pub/pdb/validation_reports/sn/6snl | HTTPS FTP |
---|
-Related structure data
Related structure data | 6xu3C 4ce5S S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
3 |
| ||||||||
Unit cell |
|
-Components
-Protein , 1 types, 6 molecules ABCDEF
#1: Protein | Mass: 38634.004 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Exophiala sideris (fungus) / Gene: PV11_02554 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: A0A0D1XFW6 |
---|
-Non-polymers , 5 types, 22 molecules
#2: Chemical | ChemComp-PLP / #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-P6G / | #6: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | Y |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.8 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5 / Details: NaCl, Bis-Tris, PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jan 19, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.129→66.44 Å / Num. obs: 43551 / % possible obs: 99 % / Redundancy: 13.1 % / Biso Wilson estimate: 53.8 Å2 / CC1/2: 0.978 / Rpim(I) all: 0.1411 / Net I/σ(I): 5.22 |
Reflection shell | Resolution: 3.129→3.241 Å / Redundancy: 13 % / Mean I/σ(I) obs: 1.08 / Num. unique obs: 4277 / CC1/2: 0.621 / Rpim(I) all: 0.6867 / % possible all: 97.36 |
-Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4CE5 Resolution: 3.129→66.44 Å / Cross valid method: FREE R-VALUE
| ||||||||||||||||
Displacement parameters | Biso mean: 50.25 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.129→66.44 Å
|