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Yorodumi- PDB-5y7l: Solution structure of Hbeta4 extracellular loop of BK potassium c... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5y7l | ||||||
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Title | Solution structure of Hbeta4 extracellular loop of BK potassium channel | ||||||
Components | Calcium-activated potassium channel subunit beta-4 | ||||||
Keywords | METAL BINDING PROTEIN / Beta4 / BK | ||||||
Function / homology | Function and homology information Ca2+ activated K+ channels / calcium-activated potassium channel activity / regulation of neurotransmitter secretion / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / action potential / cGMP effects / neuronal action potential / regulation of vasoconstriction / detection of calcium ion / potassium channel regulator activity ...Ca2+ activated K+ channels / calcium-activated potassium channel activity / regulation of neurotransmitter secretion / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / action potential / cGMP effects / neuronal action potential / regulation of vasoconstriction / detection of calcium ion / potassium channel regulator activity / voltage-gated potassium channel complex / potassium ion transport / chemical synaptic transmission / synapse / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wang, Y. / Lan, W. / Ding, J. / Cao, C. | ||||||
Citation | Journal: Sci Rep / Year: 2018 Title: Solution structure of extracellular loop of human beta 4 subunit of BK channel and its biological implication on ChTX sensitivity. Authors: Wang, Y. / Lan, W. / Yan, Z. / Gao, J. / Liu, X. / Wang, S. / Guo, X. / Wang, C. / Zhou, H. / Ding, J. / Cao, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5y7l.cif.gz | 738 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5y7l.ent.gz | 619.1 KB | Display | PDB format |
PDBx/mmJSON format | 5y7l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y7/5y7l ftp://data.pdbj.org/pub/pdb/validation_reports/y7/5y7l | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14114.560 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 45-166 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCNMB4 / Production host: Escherichia coli (E. coli) / References: UniProt: Q86W47 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 75 mM / Label: condition_1 / pH: 6.8 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz Details: a triple resonances cryoprobe and pulsed field gradients |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 3 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |