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- PDB-5l1x: Structure of the Human Metapneumovirus Fusion Protein in the Post... -

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Basic information

Entry
Database: PDB / ID: 5l1x
TitleStructure of the Human Metapneumovirus Fusion Protein in the Postfusion Conformation
Components
  • hMPV F1 subunit
  • hMPV F2 subunit
KeywordsVIRAL PROTEIN / class I fusion protein
Function / homology
Function and homology information


membrane => GO:0016020 / fusion of virus membrane with host plasma membrane / host cell plasma membrane / virion membrane / plasma membrane
Similarity search - Function
Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0
Similarity search - Domain/homology
Fusion glycoprotein F0 / Fusion glycoprotein F0 / Fusion glycoprotein F0
Similarity search - Component
Biological speciesHuman metapneumovirus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.3 Å
AuthorsMas, V. / Melero, J.A. / McLellan, J.S.
Funding support Spain, United States, 3items
OrganizationGrant numberCountry
Plan Nacional I+D+iSAF2015-67033-RSAF2012-31217 Spain
Plan Nacional I+D+iBFU 2013-43149-R Spain
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P20GM113132 United States
CitationJournal: Plos Pathog. / Year: 2016
Title: Engineering, Structure and Immunogenicity of the Human Metapneumovirus F Protein in the Postfusion Conformation.
Authors: Mas, V. / Rodriguez, L. / Olmedillas, E. / Cano, O. / Palomo, C. / Terron, M.C. / Luque, D. / Melero, J.A. / McLellan, J.S.
History
DepositionJul 29, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 24, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 21, 2016Group: Database references
Revision 1.2Sep 13, 2017Group: Author supporting evidence / Derived calculations / Category: pdbx_audit_support / pdbx_struct_oper_list
Item: _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation
Revision 1.3Dec 25, 2019Group: Author supporting evidence / Data collection / Category: chem_comp / pdbx_audit_support
Item: _chem_comp.type / _pdbx_audit_support.funding_organization
Revision 2.0Jul 29, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_validate_close_contact / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_validate_close_contact.auth_asym_id_2 / _pdbx_validate_close_contact.auth_seq_id_2 / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: hMPV F2 subunit
B: hMPV F1 subunit
C: hMPV F2 subunit
D: hMPV F1 subunit
E: hMPV F2 subunit
F: hMPV F1 subunit
G: hMPV F2 subunit
H: hMPV F1 subunit
I: hMPV F2 subunit
J: hMPV F1 subunit
K: hMPV F2 subunit
L: hMPV F1 subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)318,18029
Polymers313,50212
Non-polymers4,67817
Water0
1
A: hMPV F2 subunit
B: hMPV F1 subunit
C: hMPV F2 subunit
D: hMPV F1 subunit
E: hMPV F2 subunit
F: hMPV F1 subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)159,21115
Polymers156,7516
Non-polymers2,4609
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area55060 Å2
ΔGint-392 kcal/mol
Surface area54570 Å2
MethodPISA
2
G: hMPV F2 subunit
H: hMPV F1 subunit
I: hMPV F2 subunit
J: hMPV F1 subunit
K: hMPV F2 subunit
L: hMPV F1 subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)158,96914
Polymers156,7516
Non-polymers2,2188
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area54680 Å2
ΔGint-376 kcal/mol
Surface area54630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)128.720, 128.720, 572.790
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number92
Space group name H-MP41212
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain A
21chain C
31chain E
41chain G
51chain I
61chain K
12chain B
22chain D
32chain F
42chain H
52chain J
62chain L

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111chain AA19 - 91
211chain CC19 - 559
311chain EE19 - 91
411chain GG19 - 92
511chain II19 - 557
611chain KK19 - 92
112chain BB114 - 855
212chain DD115 - 672
312chain FF114 - 854
412chain HH114 - 854
512chain JJ115 - 672
612chain LL114 - 855

NCS ensembles :
ID
1
2

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Components

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Protein , 2 types, 12 molecules ACEGIKBDFHJL

#1: Protein
hMPV F2 subunit


Mass: 10193.454 Da / Num. of mol.: 6 / Fragment: UNP residues 19-101
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human metapneumovirus / Strain: NL/1/00 / Gene: F / Cell line (production host): CV-1 / Production host: Chlorocebus aethiops (grivet) / References: UniProt: H6X1Z1, UniProt: Q91F55*PLUS
#2: Protein
hMPV F1 subunit


Mass: 42056.820 Da / Num. of mol.: 6 / Fragment: UNP residues 112-489 / Mutation: G294E
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human metapneumovirus / Strain: NL/1/00 / Gene: F / Cell line (production host): CV-1 / Production host: Chlorocebus aethiops (grivet) / References: UniProt: Q8B9P0, UniProt: Q91F55*PLUS

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Sugars , 4 types, 12 molecules

#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE
#4: Polysaccharide alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 367.349 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-6DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,2,1/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2/a6-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE
#5: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#6: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 1 types, 5 molecules

#7: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: SO4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.08 Å3/Da / Density % sol: 69.87 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 18.5% PEG3350, 11% MPD, 0.2 M lithium sulfate, 0.01 M nickel chloride, 0.1 M imidazole

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9793 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 24, 2015
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 3.3→37.02 Å / Num. obs: 73617 / % possible obs: 99.8 % / Redundancy: 5.4 % / Biso Wilson estimate: 73.15 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.216 / Rpim(I) all: 0.099 / Rrim(I) all: 0.238 / Net I/σ(I): 5.2 / Num. measured all: 398399 / Scaling rejects: 136
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsCC1/2Diffraction-ID% possible all
3.3-3.375.61.0150.802199.9
16.17-37.024.10.0530.998189.2

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
Aimless0.5.12data scaling
PHASERphasing
PHENIXrefinement
PDB_EXTRACT3.2data extraction
iMOSFLMdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 3RRR

3rrr


Resolution: 3.3→37.019 Å / SU ML: 0.41 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.95
RfactorNum. reflection% reflection
Rfree0.2697 3654 5 %
Rwork0.2205 --
obs0.223 73071 99.01 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 195.36 Å2 / Biso mean: 76.8647 Å2 / Biso min: 27.8 Å2
Refinement stepCycle: final / Resolution: 3.3→37.019 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20242 0 299 0 20541
Biso mean--111.38 --
Num. residues----2654
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00820875
X-RAY DIFFRACTIONf_angle_d1.12328261
X-RAY DIFFRACTIONf_chiral_restr0.053411
X-RAY DIFFRACTIONf_plane_restr0.0053608
X-RAY DIFFRACTIONf_dihedral_angle_d13.6237662
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A2034X-RAY DIFFRACTION7.293TORSIONAL
12C2034X-RAY DIFFRACTION7.293TORSIONAL
13E2034X-RAY DIFFRACTION7.293TORSIONAL
14G2034X-RAY DIFFRACTION7.293TORSIONAL
15I2034X-RAY DIFFRACTION7.293TORSIONAL
16K2034X-RAY DIFFRACTION7.293TORSIONAL
21B10442X-RAY DIFFRACTION7.293TORSIONAL
22D10442X-RAY DIFFRACTION7.293TORSIONAL
23F10442X-RAY DIFFRACTION7.293TORSIONAL
24H10442X-RAY DIFFRACTION7.293TORSIONAL
25J10442X-RAY DIFFRACTION7.293TORSIONAL
26L10442X-RAY DIFFRACTION7.293TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 26

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.3-3.34340.35491430.3112566270999
3.3434-3.38920.36131360.305726912827100
3.3892-3.43760.33891180.30442605272399
3.4376-3.48880.38771450.305626652810100
3.4888-3.54330.31051130.285526362749100
3.5433-3.60140.34011240.282226702794100
3.6014-3.66340.29621430.275626352778100
3.6634-3.730.27981540.275626272781100
3.73-3.80160.32781380.259326652803100
3.8016-3.87920.28691410.239426552796100
3.8792-3.96340.27621790.217326012780100
3.9634-4.05550.28371470.212526582805100
4.0555-4.15680.26011510.214826602811100
4.1568-4.2690.24791330.205926432776100
4.269-4.39440.26211360.18482660279699
4.3944-4.5360.23651410.186926792820100
4.536-4.69790.26551400.186126822822100
4.6979-4.88560.21131350.18072680281599
4.8856-5.10740.21631140.17872708282299
5.1074-5.37590.26471500.18632693284399
5.3759-5.71160.28341460.20812677282399
5.7116-6.15070.26271260.20842735286199
6.1507-6.76630.29441510.2132713286499
6.7663-7.73760.22741500.20472730288099
7.7376-9.71930.2331500.18022772292298
9.7193-37.02110.2461500.22722711286190
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.2407-1.979-0.88141.68510.56551.3946-0.1454-0.8632-0.19420.03270.04830.70160.2745-0.68770.0870.5672-0.3904-0.03431.35030.01690.663517.087-45.3507109.8851
29.0886-3.11010.02561.40880.56067.88060.33292.2602-0.71660.1646-0.01210.12661.05440.7473-0.33930.9179-0.2912-0.05130.9992-0.21820.923134.9064-59.770762.2911
37.3378-4.26356.09846.927-5.39535.72140.30280.4743-0.2197-0.4831-0.6328-0.56960.60281.53450.36660.5712-0.22160.04420.5405-0.00950.544242.4407-54.424575.1051
41.32120.09591.26781.33871.03934.68960.0760.14640.1742-0.0499-0.24650.2441-0.40540.36310.07961.1574-0.33290.05041.2365-0.02380.571223.86-47.8775-8.4798
51.56340.16071.04390.83650.00792.2857-0.0356-0.3706-0.1090.0381-0.14520.14050.6739-0.48410.11410.5282-0.27150.04540.673-0.03750.463625.4501-50.316186.6541
60.46830.26990.34853.37640.77843.78640.0139-0.67020.1690.2295-0.03830.27580.1075-0.9980.02240.4803-0.11790.14951.4093-0.06950.687817.9333-32.7868117.0363
7-0.0358-0.1581-0.84640.8931.73057.3724-0.2591-0.01010.1293-0.36470.22040.00830.0873-0.7179-0.07370.6828-0.27610.09131.4056-0.15230.79114.2725-41.683845.8069
82.145-0.06112.17362.5149-1.5325.54650.4047-0.3897-0.5210.085-0.0332-0.03941.10120.1602-0.34960.5866-0.0606-0.00280.81170.07750.451946.7504-50.5765106.3459
96.93412.5716-2.46025.81080.86067.5318-0.73561.85860.9405-1.0785-0.2550.5267-0.69440.46150.79530.6485-0.02030.02610.92950.10890.643433.7471-32.03857.7478
109.9023.6046.22798.25354.15664.4839-1.1398-0.65951.6461-0.8911-0.47430.6544-1.7338-1.04131.59320.49950.0544-0.12410.7020.02220.536333.9212-25.914776.3094
111.61750.04790.52151.05811.52146.0567-0.3505-0.01190.0578-0.5784-0.51830.3196-0.8764-2.07910.53741.0896-0.5030.0731.1430.00270.452119.0227-48.42786.3404
120.2186-0.40030.99841.2304-0.50035.1466-0.03510.0028-0.1390.16010.0774-0.10080.5777-0.0238-0.05960.3426-0.10220.02990.64850.06230.523742.9385-44.064993.4403
133.09160.69240.38072.61060.35314.5059-0.1045-0.3258-0.25770.39620.03060.10031.1079-0.21570.03970.9356-0.1499-0.01250.74730.16770.52335.7024-57.5278115.3122
14-0.12850.31080.43730.9827-1.69296.0435-0.04630.1556-0.1466-0.1291-0.2166-0.19290.4571.5040.32570.87490.0084-0.02821.18460.01260.742430.286-58.305724.3293
159.534-5.21417.51042.8124-4.12567.0649-0.592-1.22250.63520.59750.2541-0.01360.0218-0.54060.23220.6981-0.22820.03341.0443-0.07740.466848.1522-20.0677112.1548
162.14581.2320.06511.2244-0.2180.1680.00820.04310.1963-0.0603-0.52350.3509-0.1548-1.54180.44630.32890.05170.07511.0308-0.07780.660216.3853-31.803483.4893
171.6069-1.78731.49173.0638-1.1291.7315-0.51070.5567-0.5625-0.1147-0.10610.55271.4313-1.41260.5110.598-0.4618-0.01620.867-0.07860.566913.9419-49.160775.0128
18-0.092-0.0452-0.1147-0.0054-0.62613.2614-0.08670.15140.0573-0.0392-0.01770.09770.9479-0.68980.12750.4321-0.2432-0.00721.0003-0.07620.596422.4501-41.188951.2212
192.9791-1.39881.84433.3338-1.38645.6748-0.0584-0.7173-0.17660.3597-0.07160.3324-0.3454-0.0770.12780.3643-0.12410.08080.8687-0.09530.533541.042-25.7476116.1902
207.7207-1.03911.98324.33231.34523.03940.336-0.3179-0.172-0.05930.0231-0.12720.03830.8627-0.25050.3398-0.12720.00021.0420.01060.560260.8852-32.2536109.1676
211.64460.9163.27920.27092.36898.182-0.54670.60070.0205-0.32050.08370.0166-1.43341.26190.49060.8419-0.19910.02431.1416-0.11530.650433.302-38.42232.5821
222.3302-0.15281.97071.47170.67133.276-0.0869-0.01180.0828-0.13720.28910.0446-0.4488-0.0958-0.16280.6218-0.08470.10080.34230.01340.543748.9152-22.4917-53.196
234.2149-0.1195-3.52880.83551.49735.2339-0.3701-0.328-1.05940.42110.024-0.78141.59350.13140.37670.9850.1454-0.08980.31430.09020.526552.121-40.4385-26.7086
247.3891.0254-1.04386.84253.78274.1966-0.15090.7337-0.46341.291.2861-1.21081.36562.2276-1.21271.05150.2639-0.02980.8369-0.15690.50557.8779-45.6154-43.9188
251.0910.72440.26051.7161-0.89162.88770.0754-0.24040.02760.4279-0.42060.16722.5218-0.14740.39230.4611-0.0048-0.04640.5655-0.03710.371648.4789-26.045138.5926
261.22050.27451.52771.52690.22074.4031-0.12830.16610.2022-0.28860.2182-0.0121-0.72860.413-0.05990.5395-0.01910.10650.334-0.02980.545949.8905-24.3794-50.919
274.192-0.07651.7630.26690.10523.9529-0.1230.2040.3048-0.39860.12980.1454-0.9259-0.1031-0.04041.1464-0.0001-0.06380.33120.10240.706232.0828-17.9147-74.5719
280.54910.10762.13120.1475-0.99048.7707-0.1817-0.2380.2103-0.0354-0.42630.2615-1.62430.26350.18591.26430.11080.0150.65680.01450.679841.4956-14.2741-3.3801
294.0141.0567-3.00871.31250.2765.0661-0.60680.3731-0.3997-0.08660.3354-0.25610.82660.15090.33730.69150.0889-0.09060.3374-0.10370.482750.2129-46.5948-64.0393
306.63910.70283.33631.70260.07545.9772-0.4961-1.4897-0.19231.4108-0.12680.76271.022-0.82580.6160.92610.08140.11950.58280.0290.568531.9944-33.7953-15.2974
316.55341.89695.06688.2591.26093.9059-0.3383-0.497-0.1723-0.5927-0.04521.0961-1.1336-0.83820.35940.6124-0.00350.07130.3939-0.05050.62225.7061-33.9531-33.8256
320.75070.2956-0.28272.75370.86727.1631-0.5291-0.61440.33930.153-0.87030.1818-2.1414-1.28141.08490.6695-0.13880.01810.7538-0.04880.34348.5474-18.845637.5486
330.86820.059-0.66020.79040.32754.3652-0.03130.2322-0.05250.14330.0874-0.02220.40140.3044-0.07480.6450.0712-0.05050.3095-0.0180.588943.243-42.8288-48.3255
341.79960.62620.85091.52211.12495.78370.03110.5893-0.0021-0.29340.1958-0.10980.04110.7849-0.2380.61510.09880.12610.6369-0.07430.517256.736-35.1278-68.7278
351.2370.42760.08510.06240.01422.7237-0.35810.4414-0.5489-0.5125-0.6283-0.52373.36962.10990.89631.04540.34880.09570.82-0.0580.802757.5661-31.17130.5174
369.9563-3.1219-4.86759.69544.57313.4638-0.91210.5429-0.7367-0.6662-0.26420.45992.0936-0.35320.96860.850.081-0.02950.4715-0.07270.671820.5059-50.5331-71.7163
373.49371.69492.12673.44842.18624.974-0.652-0.44250.9382-0.4304-0.06610.6398-1.2784-0.70770.65050.61550.18260.06010.3382-0.05950.551129.38-19.7102-44.0855
386.0501-0.8218-0.35116.1115-0.07430.0435-0.4474-0.25130.8256-0.0435-0.3994-0.3052-0.50140.8440.78450.722-0.03810.07170.4394-0.03330.603648.9556-13.5262-33.0367
390.11530.1037-0.4872-0.0387-0.2062.4904-0.0580.00760.10760.0568-0.08740.0978-0.4430.56090.14710.80160.1314-0.01140.2199-0.05750.52940.2716-22.6539-10.1947
403.17080.0902-0.80021.05421.53725.2872-0.01390.30270.1951-0.36550.0388-0.1276-0.1591-0.4452-0.02360.7640.0178-0.14020.35380.11140.588526.2701-41.9655-74.4346
416.5586-0.821.39938.2363-2.42560.9476-0.00450.4739-0.34570.7309-0.0377-0.61521.3148-0.26320.06031.28050.0256-0.13920.409-0.07850.528831.6484-61.0138-66.0959
420.53010.8721.99311.62012.90112.85640.0855-0.1893-0.09330.0595-0.26080.05860.6148-0.81870.17980.8867-0.0813-0.04810.50640.05210.554338.5161-32.772411.4068
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 19:49)A19 - 49
2X-RAY DIFFRACTION2(chain A and resid 50:62)A50 - 62
3X-RAY DIFFRACTION3(chain A and resid 63:91)A63 - 91
4X-RAY DIFFRACTION4(chain B and resid 114:166)B114 - 166
5X-RAY DIFFRACTION5(chain B and resid 167:301)B167 - 301
6X-RAY DIFFRACTION6(chain B and resid 302:418)B302 - 418
7X-RAY DIFFRACTION7(chain B and resid 419:482)B419 - 482
8X-RAY DIFFRACTION8(chain C and resid 19:50)C19 - 50
9X-RAY DIFFRACTION9(chain C and resid 51:68)C51 - 68
10X-RAY DIFFRACTION10(chain C and resid 69:91)C69 - 91
11X-RAY DIFFRACTION11(chain D and resid 115:184)D115 - 184
12X-RAY DIFFRACTION12(chain D and resid 185:310)D185 - 310
13X-RAY DIFFRACTION13(chain D and resid 311:435)D311 - 435
14X-RAY DIFFRACTION14(chain D and resid 436:483)D436 - 483
15X-RAY DIFFRACTION15(chain E and resid 19:37)E19 - 37
16X-RAY DIFFRACTION16(chain E and resid 38:58)E38 - 58
17X-RAY DIFFRACTION17(chain E and resid 59:91)E59 - 91
18X-RAY DIFFRACTION18(chain F and resid 114:276)F114 - 276
19X-RAY DIFFRACTION19(chain F and resid 277:368)F277 - 368
20X-RAY DIFFRACTION20(chain F and resid 369:427)F369 - 427
21X-RAY DIFFRACTION21(chain F and resid 428:482)F428 - 482
22X-RAY DIFFRACTION22(chain G and resid 19:64)G19 - 64
23X-RAY DIFFRACTION23(chain G and resid 65:80)G65 - 80
24X-RAY DIFFRACTION24(chain G and resid 81:92)G81 - 92
25X-RAY DIFFRACTION25(chain H and resid 114:182)H114 - 182
26X-RAY DIFFRACTION26(chain H and resid 183:301)H183 - 301
27X-RAY DIFFRACTION27(chain H and resid 302:418)H302 - 418
28X-RAY DIFFRACTION28(chain H and resid 419:482)H419 - 482
29X-RAY DIFFRACTION29(chain I and resid 19:50)I19 - 50
30X-RAY DIFFRACTION30(chain I and resid 51:68)I51 - 68
31X-RAY DIFFRACTION31(chain I and resid 69:91)I69 - 91
32X-RAY DIFFRACTION32(chain J and resid 115:182)J115 - 182
33X-RAY DIFFRACTION33(chain J and resid 183:302)J183 - 302
34X-RAY DIFFRACTION34(chain J and resid 303:448)J303 - 448
35X-RAY DIFFRACTION35(chain J and resid 449:482)J449 - 482
36X-RAY DIFFRACTION36(chain K and resid 19:33)K19 - 33
37X-RAY DIFFRACTION37(chain K and resid 34:58)K34 - 58
38X-RAY DIFFRACTION38(chain K and resid 59:92)K59 - 92
39X-RAY DIFFRACTION39(chain L and resid 114:281)L114 - 281
40X-RAY DIFFRACTION40(chain L and resid 282:370)L282 - 370
41X-RAY DIFFRACTION41(chain L and resid 371:428)L371 - 428
42X-RAY DIFFRACTION42(chain L and resid 429:483)L429 - 483

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