Roquin-2 / Membrane-associated nucleic acid-binding protein / RING finger and CCCH-type zinc finger domain- ...Membrane-associated nucleic acid-binding protein / RING finger and CCCH-type zinc finger domain-containing protein 2 / RING finger protein 164
Mass: 17593.250 Da / Num. of mol.: 4 / Fragment: UNP residues 171-325 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RC3H2, MNAB, RNF164 / Plasmid: pGEX6P-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pLysS / References: UniProt: Q9HBD1
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 2.7→50 Å / Num. obs: 17819 / % possible obs: 99.1 % / Redundancy: 12.3 % / Rsym value: 0.169 / Net I/σ(I): 14.4
-
Processing
Software
Name
Version
Classification
REFMAC
5.8.0049
refinement
HKL-2000
datareduction
HKL-2000
datascaling
MOLREP
phasing
Refinement
Resolution: 2.7→49.438 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.916 / SU B: 31.751 / SU ML: 0.3 / Cross valid method: THROUGHOUT / ESU R Free: 0.381 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.26009
910
5.1 %
RANDOM
Rwork
0.2241
-
-
-
obs
0.22596
16909
99.05 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK