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- PDB-4yr8: Crystal structure of JNK in complex with a regulator protein -

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Basic information

Entry
Database: PDB / ID: 4yr8
TitleCrystal structure of JNK in complex with a regulator protein
Components
  • Dual specificity protein phosphatase 16
  • Mitogen-activated protein kinase 8
KeywordsTRANSFERASE/HYDROLASE / kinase domain / catalytic domain / TRANSFERASE-HYDROLASE complex
Function / homology
Function and homology information


: / MAP kinase tyrosine phosphatase activity / positive regulation of cell killing / JUN phosphorylation / protein tyrosine/threonine phosphatase activity / regulation of DNA replication origin binding / Interleukin-38 signaling / Activation of BMF and translocation to mitochondria / basal dendrite / MAP kinase tyrosine/serine/threonine phosphatase activity ...: / MAP kinase tyrosine phosphatase activity / positive regulation of cell killing / JUN phosphorylation / protein tyrosine/threonine phosphatase activity / regulation of DNA replication origin binding / Interleukin-38 signaling / Activation of BMF and translocation to mitochondria / basal dendrite / MAP kinase tyrosine/serine/threonine phosphatase activity / Suppression of autophagy / Activation of BIM and translocation to mitochondria / JUN kinase activity / WNT5:FZD7-mediated leishmania damping / Signaling by MAPK mutants / negative regulation of JUN kinase activity / RAF-independent MAPK1/3 activation / JUN kinase binding / positive regulation of cyclase activity / mitogen-activated protein kinase binding / histone deacetylase regulator activity / positive regulation of NLRP3 inflammasome complex assembly / DSCAM interactions / myosin phosphatase activity / protein serine/threonine kinase binding / mitogen-activated protein kinase p38 binding / NRAGE signals death through JNK / negative regulation of MAPK cascade / protein-serine/threonine phosphatase / Activation of the AP-1 family of transcription factors / phosphatase activity / Fc-epsilon receptor signaling pathway / phosphoprotein phosphatase activity / regulation of macroautophagy / mitogen-activated protein kinase / stress-activated MAPK cascade / response to mechanical stimulus / dephosphorylation / response to UV / JNK cascade / cellular response to cadmium ion / cellular response to amino acid starvation / positive regulation of protein metabolic process / NRIF signals cell death from the nucleus / protein dephosphorylation / protein-tyrosine-phosphatase / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / negative regulation of protein binding / FCERI mediated MAPK activation / peptidyl-threonine phosphorylation / regulation of circadian rhythm / cellular response to reactive oxygen species / cellular response to mechanical stimulus / histone deacetylase binding / Negative regulation of MAPK pathway / rhythmic process / regulation of protein localization / cellular senescence / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / cellular response to oxidative stress / cytoplasmic vesicle / peptidyl-serine phosphorylation / protein phosphatase binding / Oxidative Stress Induced Senescence / response to oxidative stress / cellular response to lipopolysaccharide / positive regulation of apoptotic process / axon / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / synapse / positive regulation of gene expression / negative regulation of apoptotic process / enzyme binding / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Mitogen-activated protein (MAP) kinase phosphatase / Mitogen-activated protein (MAP) kinase, JNK / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Rhodanese Homology Domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Rhodanese-like domain / Rhodanese domain profile. ...Mitogen-activated protein (MAP) kinase phosphatase / Mitogen-activated protein (MAP) kinase, JNK / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Rhodanese Homology Domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Rhodanese-like domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / Rhodanese-like domain / Protein tyrosine phosphatase superfamily / Protein-Tyrosine Phosphatase; Chain A / Mitogen-activated protein (MAP) kinase, conserved site / MAP kinase signature. / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Protein-tyrosine phosphatase-like / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Alpha-Beta Complex / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Mitogen-activated protein kinase 8 / Dual specificity protein phosphatase 16
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å
AuthorsLiu, X. / Wang, J. / Wu, J.W. / Wang, Z.X.
Funding support China, 4items
OrganizationGrant numberCountry
Ministry of Science and Technology2013CB530600 China
Ministry of Science and Technology2011CB910800 China
National Natural Science Foundation of China31270848 China
National Natural Science Foundation of China31130062 China
CitationJournal: Nat Commun / Year: 2016
Title: A conserved motif in JNK/p38-specific MAPK phosphatases as a determinant for JNK1 recognition and inactivation.
Authors: Liu, X. / Zhang, C.S. / Lu, C. / Lin, S.C. / Wu, J.W. / Wang, Z.X.
History
DepositionMar 14, 2015Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Mar 16, 2016Provider: repository / Type: Initial release
Revision 1.1Mar 30, 2016Group: Database references
Revision 1.2Nov 8, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: Mitogen-activated protein kinase 8
G: Dual specificity protein phosphatase 16
A: Mitogen-activated protein kinase 8
B: Dual specificity protein phosphatase 16
C: Mitogen-activated protein kinase 8
D: Dual specificity protein phosphatase 16
F: Mitogen-activated protein kinase 8
H: Dual specificity protein phosphatase 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)247,13912
Polymers246,9978
Non-polymers1424
Water6,413356
1
E: Mitogen-activated protein kinase 8
G: Dual specificity protein phosphatase 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,7853
Polymers61,7492
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1470 Å2
ΔGint-18 kcal/mol
Surface area21640 Å2
MethodPISA
2
A: Mitogen-activated protein kinase 8
B: Dual specificity protein phosphatase 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,7853
Polymers61,7492
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1530 Å2
ΔGint-18 kcal/mol
Surface area21620 Å2
MethodPISA
3
C: Mitogen-activated protein kinase 8
D: Dual specificity protein phosphatase 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,7853
Polymers61,7492
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1470 Å2
ΔGint-19 kcal/mol
Surface area21790 Å2
MethodPISA
4
F: Mitogen-activated protein kinase 8
H: Dual specificity protein phosphatase 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,7853
Polymers61,7492
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1400 Å2
ΔGint-19 kcal/mol
Surface area21220 Å2
MethodPISA
Unit cell
Length a, b, c (Å)58.151, 74.850, 134.810
Angle α, β, γ (deg.)76.94, 84.32, 67.44
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
Mitogen-activated protein kinase 8 / MAPK 8 / JNK-46 / Stress-activated protein kinase 1c / SAPK1c / Stress-activated protein kinase ...MAPK 8 / JNK-46 / Stress-activated protein kinase 1c / SAPK1c / Stress-activated protein kinase JNK1 / c-Jun N-terminal kinase 1


Mass: 42830.461 Da / Num. of mol.: 4 / Fragment: UNP RESIDUES 1-363
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MAPK8, JNK1, PRKM8, SAPK1, SAPK1C / Plasmid: PET21b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: P45983, mitogen-activated protein kinase
#2: Protein
Dual specificity protein phosphatase 16 / Mitogen-activated protein kinase phosphatase 7 / MKP-7


Mass: 18918.904 Da / Num. of mol.: 4 / Fragment: UNP RESIDUES 156-301
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DUSP16, KIAA1700, MKP7 / Plasmid: PET15b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: Q9BY84, protein-serine/threonine phosphatase, protein-tyrosine-phosphatase
#3: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 356 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsSEQUENCE OF THE CHAINS A,C,E,F WAS BASED ON ISOFORM 4 OF DATABASE P45983

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.14 Å3/Da / Density % sol: 42.44 %
Crystal growTemperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 0.1M HEPES, pH 7.0, 14% PEG 3350, 0.2M MgCl2, 6% 1,6-Hexanediol, 0.005M EDTA

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9793 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 5, 2012
RadiationMonochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 2.4→40 Å / Num. obs: 78865 / % possible obs: 99.6 % / Redundancy: 3.5 % / Biso Wilson estimate: 28.04 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 14.1
Reflection shellResolution: 2.4→2.49 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.609 / Mean I/σ(I) obs: 1.9 / % possible all: 99.9

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Processing

SoftwareName: PHENIX / Version: 1.8.4_1496 / Classification: refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1UKH, 1ZZW

1ukh

1zzw


Resolution: 2.4→38.02 Å / SU ML: 0.29 / σ(F): 1.97 / Phase error: 27.3 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.239 3405 5.09 %
Rwork0.217 --
obs0.218 66889 83.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 46.81 Å2
Refinement stepCycle: LAST / Resolution: 2.4→38.02 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms14615 0 4 356 14975
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01514948
X-RAY DIFFRACTIONf_angle_d1.56420185
X-RAY DIFFRACTIONf_dihedral_angle_d16.725675
X-RAY DIFFRACTIONf_chiral_restr0.1162240
X-RAY DIFFRACTIONf_plane_restr0.0082566
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4012-2.43550.311890.2151181X-RAY DIFFRACTION6
2.4355-2.47190.269220.2223399X-RAY DIFFRACTION13
2.4719-2.51050.2844440.2699932X-RAY DIFFRACTION29
2.5105-2.55170.2774660.26961563X-RAY DIFFRACTION49
2.5517-2.59570.31841310.29172262X-RAY DIFFRACTION72
2.5957-2.64280.2951410.28362508X-RAY DIFFRACTION80
2.6428-2.69370.28161250.2712746X-RAY DIFFRACTION87
2.6937-2.74860.2931410.2692881X-RAY DIFFRACTION91
2.7486-2.80840.31151640.2833049X-RAY DIFFRACTION96
2.8084-2.87370.25661600.26893144X-RAY DIFFRACTION98
2.8737-2.94550.31781730.27453105X-RAY DIFFRACTION99
2.9455-3.02510.26441530.25783167X-RAY DIFFRACTION100
3.0251-3.11410.2751730.25533138X-RAY DIFFRACTION100
3.1141-3.21460.28031810.24083163X-RAY DIFFRACTION100
3.2146-3.32940.27591610.23543102X-RAY DIFFRACTION100
3.3294-3.46260.25041590.22453185X-RAY DIFFRACTION100
3.4626-3.62010.22011780.19783139X-RAY DIFFRACTION100
3.6201-3.81080.20991850.18883114X-RAY DIFFRACTION99
3.8108-4.04930.211710.18353117X-RAY DIFFRACTION99
4.0493-4.36150.19951530.17563170X-RAY DIFFRACTION99
4.3615-4.79970.19021850.16863108X-RAY DIFFRACTION99
4.7997-5.49240.2251950.18733099X-RAY DIFFRACTION100
5.4924-6.91310.23671630.21493182X-RAY DIFFRACTION100
6.9131-38.02170.19671720.18943030X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.02024.7003-1.95534.063-0.26075.0492-0.2063-0.1546-0.0976-0.0745-0.0123-0.71750.10410.18630.14930.35780.2148-0.00780.37410.00950.332-23.253452.264590.1022
22.84271.48791.01552.05910.61252.3177-0.20930.12610.0942-0.27070.1294-0.20810.2350.13110.03910.4650.1681-0.02930.3269-0.03540.3421-34.499750.295282.8564
30.88010.39731.38954.65620.43232.2236-0.07610.22910.3805-0.5590.1429-0.6471-0.14630.1541-0.1790.42420.1672-0.06840.3056-0.04570.3595-28.741950.101782.5422
41.07580.1576-0.14971.34320.74990.8804-0.2796-0.15370.0679-0.19380.0490.032-0.16370.07830.07860.38120.3213-0.07760.37960.03090.218-53.380945.067385.6406
51.35841.26070.63562.37041.26550.7803-0.23580.3160.0079-0.28510.3415-0.5453-0.14770.28190.05370.48510.1205-0.00920.3405-0.02040.254-48.2732.181776.5068
61.48570.57880.20461.45860.36370.6162-0.22620.02580.0794-0.16240.06730.2476-0.1761-0.17220.06950.40250.1896-0.05060.29460.00610.1439-58.901332.948580.7786
74.86040.08610.91712.1470.0612.7181-0.2133-0.0897-0.16110.359-0.0923-1.0493-0.08451.66510.2270.38050.0361-0.02911.00530.15540.6016-24.099614.232585.364
83.7822-0.90751.36952.4623-0.19381.9474-0.11930.4209-0.2431-0.2356-0.2146-0.97180.2321.73430.1180.35060.16190.09661.09620.08370.5166-27.13511.186472.7788
93.91752.2047-0.10082.6051-0.96611.939-0.07740.0858-0.4738-0.05870.0393-0.18850.35320.7060.07030.29070.11120.02510.3681-0.04580.2933-35.800610.717977.7333
103.9747-0.55961.3135.2948-0.35775.1807-0.0916-0.2688-0.7030.31280.13070.0170.38710.27430.10190.22490.09450.01450.2570.06020.2497-41.882310.100784.0837
114.5236-0.34340.2796.5676-0.55064.9154-0.00890.5915-0.8026-0.6511-0.0260.09150.60380.410.07620.33570.0590.00220.3194-0.06760.2299-45.15018.5171.4721
122.9025-1.3388-0.59281.88510.22030.56610.0198-0.24-0.16140.14290.0949-0.14450.08790.1288-0.0060.2701-0.2195-0.00220.24420.03770.2604-51.9174-13.526840.2827
131.6406-0.8482-0.17261.91310.41260.946-0.0985-0.2674-0.07020.20450.08370.18250.1427-0.137-0.04550.2836-0.18950.06070.270.010.1798-69.51471.884645.109
143.56070.61920.29032.4524-1.28163.7661-0.15510.45030.208-0.34570.1671-0.75390.24871.51020.14770.3033-0.04380.04560.7524-0.010.376-37.183621.949440.0633
154.94410.6617-0.11761.30610.02561.5255-0.0878-0.1589-0.17090.0204-0.2392-0.9398-0.02421.53990.15980.3015-0.0105-0.0190.95070.04950.3709-33.670320.218451.8282
163.36250.4479-0.04422.69950.11382.8852-0.08620.04960.47650.27410.0475-0.3404-0.40440.79770.03140.2341-0.1143-0.0520.3256-0.03790.2558-45.971525.352850.3241
173.76640.3855-0.27574.955-0.57455.762-0.00560.40740.7183-0.43810.17710.2534-0.4382-0.0377-0.02120.1318-0.0516-0.01440.1980.06640.1839-55.189724.876440.5988
185.10140.75820.69837.7851-0.56896.65090.0118-0.520.85660.67810.06930.3837-0.7174-0.3199-0.03710.24280.00140.0320.2352-0.06020.243-58.258526.491453.2133
191.9266-0.5017-0.78311.58140.5293.83180.31460.5554-0.3815-0.3396-0.17480.070.62770.28360.06140.60050.0239-0.11410.4858-0.00240.4236-42.811827.05813.943
202.36920.1184-0.64071.7532-0.00592.08570.08410.176-0.0039-0.1979-0.05820.12550.0262-0.0154-0.03140.3238-0.1453-0.07670.15920.07140.2803-58.100637.9722.6825
215.9732-0.5487-1.84518.3273-1.33054.19780.4111.7201-0.614-0.41790.1491-0.0641.38990.3444-0.28810.71360.0998-0.26271.1693-0.00640.4827-49.048332.5078-10.3079
225.74812.03410.79826.02462.11563.51110.03640.103-0.5746-0.10180.19980.17580.9332-0.0909-0.20790.7013-0.1956-0.03410.2809-0.05890.286-71.97222.351319.1525
233.5078-0.04571.01333.9991.91454.68090.01230.6772-0.7924-0.60580.3758-0.52171.20970.6532-0.3921.18090.09130.0130.5799-0.22360.4575-64.7458-1.52129.0596
242.15020.73320.93412.95050.68563.0241-0.24030.6427-0.0302-1.15760.23690.69550.7172-0.1793-0.04690.8471-0.3179-0.16920.50960.04880.3682-76.998.13079.9298
256.07-1.2530.05646.25280.15775.4967-0.139-0.38020.10560.49590.23320.82640.4499-0.3548-0.03080.4355-0.15560.06320.2555-0.0380.247-74.829614.023626.5046
263.1078-0.01691.19375.43040.25424.8383-0.33440.19760.3816-0.53210.10490.9353-0.0479-0.51760.10570.413-0.1677-0.12360.32930.05830.3517-77.629119.929318.3365
273.12340.70821.04661.2451-0.18752.69730.3332-0.52310.45330.3788-0.3185-0.0447-0.41480.29210.04160.55230.04790.04410.4194-0.05120.3361-32.22095.9334116.8767
280.7110.4383-0.00440.92890.26030.58890.2718-0.0921-0.02270.242-0.1190.04010.1343-0.0090.07810.40940.36690.00490.20210.02450.2681-43.7124-3.5081104.8284
294.5419-1.0397-0.23272.29831.28346.55540.0303-0.0250.36450.14070.14660.0309-1.3727-0.0136-0.03970.79390.175-0.00060.3438-0.01040.1051-58.70832.6856105.8307
301.51950.99290.18090.71270.12690.02280.1646-0.61240.45730.19690.0944-0.0204-0.42010.431-0.23491.1231-0.0066-0.110.5503-0.20340.3692-51.7636.5734115.8968
311.6371-0.4372-1.21982.00870.44132.795-0.1139-0.51750.17540.75540.21020.2404-0.5896-0.093-0.04371.12040.43730.25540.5739-0.06190.2471-63.89827.9808117.2488
322.44491.23772.68292.43031.24594.12710.2067-0.08360.09210.0427-0.13860.4307-0.0769-0.2616-0.1390.4880.2420.0480.3849-0.00990.2496-62.077722.8227108.9192
336.26751.1967-0.3966.2726-0.34065.1924-0.20390.51250.0227-0.57440.14530.6262-0.2993-0.3690.04170.51810.1351-0.06630.28590.01110.1974-62.040421.376998.4282
344.4388-0.2053-0.76095.2585-0.02614.5805-0.3034-0.1821-0.41530.31720.17030.89810.0796-0.50430.15010.42020.20710.09740.31690.05610.316-64.716915.0261106.4843
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'E' AND (RESID 7 THROUGH 23 )
2X-RAY DIFFRACTION2CHAIN 'E' AND (RESID 24 THROUGH 92 )
3X-RAY DIFFRACTION3CHAIN 'E' AND (RESID 93 THROUGH 114 )
4X-RAY DIFFRACTION4CHAIN 'E' AND (RESID 115 THROUGH 167 )
5X-RAY DIFFRACTION5CHAIN 'E' AND (RESID 168 THROUGH 203 )
6X-RAY DIFFRACTION6CHAIN 'E' AND (RESID 204 THROUGH 362 )
7X-RAY DIFFRACTION7CHAIN 'G' AND (RESID 158 THROUGH 183 )
8X-RAY DIFFRACTION8CHAIN 'G' AND (RESID 184 THROUGH 243 )
9X-RAY DIFFRACTION9CHAIN 'G' AND (RESID 244 THROUGH 262 )
10X-RAY DIFFRACTION10CHAIN 'G' AND (RESID 263 THROUGH 284 )
11X-RAY DIFFRACTION11CHAIN 'G' AND (RESID 285 THROUGH 298 )
12X-RAY DIFFRACTION12CHAIN 'A' AND (RESID 8 THROUGH 167 )
13X-RAY DIFFRACTION13CHAIN 'A' AND (RESID 168 THROUGH 362 )
14X-RAY DIFFRACTION14CHAIN 'B' AND (RESID 158 THROUGH 190 )
15X-RAY DIFFRACTION15CHAIN 'B' AND (RESID 191 THROUGH 208 )
16X-RAY DIFFRACTION16CHAIN 'B' AND (RESID 209 THROUGH 262 )
17X-RAY DIFFRACTION17CHAIN 'B' AND (RESID 263 THROUGH 284 )
18X-RAY DIFFRACTION18CHAIN 'B' AND (RESID 285 THROUGH 298 )
19X-RAY DIFFRACTION19CHAIN 'C' AND (RESID 10 THROUGH 167 )
20X-RAY DIFFRACTION20CHAIN 'C' AND (RESID 168 THROUGH 330 )
21X-RAY DIFFRACTION21CHAIN 'C' AND (RESID 331 THROUGH 362 )
22X-RAY DIFFRACTION22CHAIN 'D' AND (RESID 158 THROUGH 169 )
23X-RAY DIFFRACTION23CHAIN 'D' AND (RESID 170 THROUGH 182 )
24X-RAY DIFFRACTION24CHAIN 'D' AND (RESID 183 THROUGH 263 )
25X-RAY DIFFRACTION25CHAIN 'D' AND (RESID 264 THROUGH 277 )
26X-RAY DIFFRACTION26CHAIN 'D' AND (RESID 278 THROUGH 299 )
27X-RAY DIFFRACTION27CHAIN 'F' AND (RESID 10 THROUGH 220 )
28X-RAY DIFFRACTION28CHAIN 'F' AND (RESID 221 THROUGH 362 )
29X-RAY DIFFRACTION29CHAIN 'H' AND (RESID 158 THROUGH 169 )
30X-RAY DIFFRACTION30CHAIN 'H' AND (RESID 170 THROUGH 182 )
31X-RAY DIFFRACTION31CHAIN 'H' AND (RESID 183 THROUGH 243 )
32X-RAY DIFFRACTION32CHAIN 'H' AND (RESID 244 THROUGH 262 )
33X-RAY DIFFRACTION33CHAIN 'H' AND (RESID 263 THROUGH 277 )
34X-RAY DIFFRACTION34CHAIN 'H' AND (RESID 278 THROUGH 300 )

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