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Yorodumi- PDB-4f8c: Structure of the Cif:Nedd8 complex - Yersinia pseudotuberculosis ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4f8c | ||||||
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Title | Structure of the Cif:Nedd8 complex - Yersinia pseudotuberculosis Cycle Inhibiting Factor in complex with human Nedd8 | ||||||
Components |
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Keywords | CELL CYCLE/PROTEIN BINDING / Effector-Host target complex / Glutamine deamidase / Deamidation / BACTERIAL EFFECTOR / CELL CYCLE-PROTEIN BINDING complex | ||||||
Function / homology | Function and homology information regulation of proteolysis / protein neddylation / TGF-beta receptor signaling activates SMADs / anatomical structure morphogenesis / Iron uptake and transport / protein modification process / protein localization / modification-dependent protein catabolic process / protein tag activity / UCH proteinases ...regulation of proteolysis / protein neddylation / TGF-beta receptor signaling activates SMADs / anatomical structure morphogenesis / Iron uptake and transport / protein modification process / protein localization / modification-dependent protein catabolic process / protein tag activity / UCH proteinases / Cargo recognition for clathrin-mediated endocytosis / Neddylation / ubiquitin-dependent protein catabolic process / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / proteolysis / extracellular exosome / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Yersinia pseudotuberculosis (bacteria) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | ||||||
Authors | Crow, A. / Banfield, M. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: The molecular basis of Nedd8 deamidation by the bacterial effector protein Cif Authors: Crow, A. / Hughes, R.K. / Taieb, F. / Oswald, E. / Banfield, M.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4f8c.cif.gz | 151.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4f8c.ent.gz | 120.3 KB | Display | PDB format |
PDBx/mmJSON format | 4f8c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f8/4f8c ftp://data.pdbj.org/pub/pdb/validation_reports/f8/4f8c | HTTPS FTP |
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-Related structure data
Related structure data | 4fbjSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 30809.465 Da / Num. of mol.: 2 / Fragment: Effector domain, UNP residues 33-288 / Mutation: C117A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Yersinia pseudotuberculosis (bacteria) / Strain: YPIII / Gene: YPK_1971 / Production host: Escherichia coli (E. coli) / Strain (production host): SoluBL21 / References: UniProt: B1JJZ9, UniProt: A0A0H3B1Q8*PLUS #2: Protein | Mass: 10044.623 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NEDD8 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Rossetta / References: UniProt: Q15843 #3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.85 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 2.2M sodium malonate, 44mM bis-tris propane (pH 7.0), 66mM bis-tris propane (pH 8.0), VAPOR DIFFUSION, SITTING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 1.0072 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 14, 2011 |
Radiation | Monochromator: Double Crystal Monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0072 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→66.9 Å / Num. all: 57931 / Num. obs: 57931 / % possible obs: 100 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 11.1 % / Rsym value: 0.079 / Net I/σ(I): 18.5 |
Reflection shell | Resolution: 1.95→2.06 Å / Redundancy: 9.2 % / Mean I/σ(I) obs: 4.2 / Rsym value: 0.463 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4FBJ Resolution: 1.95→58.81 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.93 / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / ESU R: 0.157 / ESU R Free: 0.151 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.35 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→58.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.001 Å / Total num. of bins used: 20
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