- PDB-4aki: Dynein Motor Domain - LuAc derivative -
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基本情報
登録情報
データベース: PDB / ID: 4aki
タイトル
Dynein Motor Domain - LuAc derivative
要素
GLUTATHIONE S-TRANSFERASE CLASS-MU 26 KDA ISOZYME, DYNEIN HEAVY CHAIN CYTOPLASMIC
キーワード
MOTOR PROTEIN (モータータンパク質) / DYNEIN (ダイニン) / DYNEIN HEAVY CHAIN (ダイニン) / DYNEIN MOTOR DOMAIN (モータータンパク質) / MOTOR PROTEIN AAA+ PROTEIN (機関 (機械)) / ASCE PROTEIN / P-LOOP NTPASE / CYTOSKELETAL MOTOR
機能・相同性
機能・相同性情報
karyogamy / establishment of mitotic spindle localization / astral microtubule / nuclear migration along microtubule / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / spindle pole body / nuclear migration / dynein intermediate chain binding ...karyogamy / establishment of mitotic spindle localization / astral microtubule / nuclear migration along microtubule / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / spindle pole body / nuclear migration / dynein intermediate chain binding / cytoplasmic microtubule / mitotic sister chromatid segregation / establishment of mitotic spindle orientation / グルタチオン-S-トランスフェラーゼ / glutathione transferase activity / cytoplasmic microtubule organization / Neutrophil degranulation / mitotic spindle organization / 細胞皮質 / ATP hydrolysis activity / ATP binding / 細胞質 類似検索 - 分子機能
Helicase, Ruva Protein; domain 3 - #740 / Monooxygenase - #160 / Dynein motor, AAA2 domain, small subdomain / Dynein motor, AAA1 domain, small subdomain / Region D6 of dynein motor / Dynein motor heavy chain, linker domain, N-terminal subdomain / Dynein motor heavy chain, linker domain, subdomain 4 / Histone Acetyltransferase; Chain A - #20 / Histone Acetyltransferase; Chain A - #30 / Dynein motor heavy chain, linker domain, subdomain 3 ...Helicase, Ruva Protein; domain 3 - #740 / Monooxygenase - #160 / Dynein motor, AAA2 domain, small subdomain / Dynein motor, AAA1 domain, small subdomain / Region D6 of dynein motor / Dynein motor heavy chain, linker domain, N-terminal subdomain / Dynein motor heavy chain, linker domain, subdomain 4 / Histone Acetyltransferase; Chain A - #20 / Histone Acetyltransferase; Chain A - #30 / Dynein motor heavy chain, linker domain, subdomain 3 / Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2 - #130 / Split barrel-like / : / DYN1, AAA+ ATPase lid domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Monooxygenase / Glutathione S-transferase, C-terminal domain / Butyryl-CoA Dehydrogenase, subunit A; domain 3 / Cyclin A; domain 1 / Glutathione S-transferase, N-terminal domain / Glutathione transferase family / Glutathione S-transferase, C-terminal / Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2 / Soluble glutathione S-transferase N-terminal domain profile. / Glutathione S-transferase, C-terminal-like / Soluble glutathione S-transferase C-terminal domain profile. / Glutathione S-transferase, N-terminal / Glutathione S-transferase, C-terminal domain superfamily / Helicase, Ruva Protein; domain 3 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Histone Acetyltransferase; Chain A / Thioredoxin-like superfamily / P-loop containing nucleotide triphosphate hydrolases / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Alpha-Beta Barrel / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / ロスマンフォールド / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.34072 Å / 相対比: 1
反射
解像度: 3.7→49.13 Å / Num. obs: 88918 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / 冗長度: 9.9 % / Rmerge(I) obs: 0.15 / Net I/σ(I): 9.4
反射 シェル
解像度: 3.7→3.77 Å / 冗長度: 8.1 % / Mean I/σ(I) obs: 2 / % possible all: 99.7
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.7.0019
精密化
MOSFLM
データ削減
Aimless
データスケーリング
精密化
構造決定の手法: 単波長異常分散 開始モデル: NONE 解像度: 3.7→49.14 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.913 / SU B: 41.941 / SU ML: 0.626 / 交差検証法: THROUGHOUT / ESU R Free: 0.764 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.28903
4446
5 %
RANDOM
Rwork
0.23053
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obs
0.2335
84466
99.82 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK