PDB-3u61: Structure of T4 Bacteriophage Clamp Loader Bound To Closed Clamp,...

ID or keywords:

Entry

Database: PDB / ID: 3u61

Title

Structure of T4 Bacteriophage Clamp Loader Bound To Closed Clamp, DNA and ATP Analog and ADP

Components

(DNA polymerase accessory protein ...) x 2
DNA polymerase processivity component
Primer DNA strand
Template DNA strand

Keywords

DNA BINDING PROTEIN/DNA /

AAA+ / ATP hydrolase / clamp loader /

sliding clamp / primer-template DNA / DNA BINDING PROTEIN-DNA complex

Function / homology

Function and homology information

Rad17 RFC-like complex / Elg1 RFC-like complex / DNA replication factor C complex / DNA clamp loader activity / bidirectional double-stranded viral DNA replication / viral DNA genome replication / DNA polymerase processivity factor activity / viral transcription /

Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA-templated DNA replication ...
Similarity search - Function

Biological species

Enterobacteria phage T4 (virus)

Method

X-RAY DIFFRACTION /

SYNCHROTRON / molecular replacementSAD / Resolution: 3.2 Å

Authors

Kelch, B.A. / Makino, D.L. / O'Donnell, M. / Kuriyan, J.

Citation

Journal: Science / Year: 2011
Title: How a DNA polymerase clamp loader opens a sliding clamp.
Authors: Kelch, B.A. / Makino, D.L. / O'Donnell, M. / Kuriyan, J.

History

Deposition	Oct 11, 2011	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jan 4, 2012	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	3u61.cif.gz	854.2 KB	Display	PDBx/mmCIF format
PDB format	pdb3u61.ent.gz	733 KB	Display	PDB format
PDBx/mmJSON format	3u61.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/u6/3u61 ftp://data.pdbj.org/pub/pdb/validation_reports/u6/3u61	HTTPS FTP

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Related structure data

Related structure data	3u5zC 3u60C 1czd 1jr3 1sxj 1xxh 1xxi 3glf 3glg C: citing same article (ref.)
Similar structure data	6fua-1 2976 6ftt-d 7a5v-1 7cg0-d 0310 4nsc-d 1k90-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#150 - Jun 2012 Sliding Clamps similarity (14) #67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #253 - Jan 2021 Expressome similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #135 - Mar 2011 Integrase similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #84 - Dec 2006 Transposase similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #125 - May 2010 Parvoviruses similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #131 - Nov 2010 Inteins similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #23 - Nov 2001 DNA similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

B: DNA polymerase accessory protein 44

C: DNA polymerase accessory protein 44

D: DNA polymerase accessory protein 44

E: DNA polymerase accessory protein 44

A: DNA polymerase accessory protein 62

G: DNA polymerase processivity component

H: DNA polymerase processivity component

F: DNA polymerase processivity component

I: Template DNA strand

J: Primer DNA strand

hetero molecules

254 kDa, 10 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	82.303, 137.272, 222.779
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Selection details
1	1	1	chain F and resid 1001:1101
2	1	1	chain G and resid 2001:2101
3	1	1	chain H and resid 3001:3101
1	1	2	chain F and resid 1102:1220
2	1	2	chain G and resid 2102:2220
3	1	2	chain H and resid 3102:3220
1	1	3	chain B and resid 6:159
2	1	3	chain C and resid 6:159
1	1	4	chain B and resid 160:208
2	1	4	chain C and resid 160:208
1	1	5	chain A and resid 209:218
2	1	5	chain B and resid 209:218
3	1	5	chain C and resid 209:218
1	1	6	chain A and resid 234:316
2	1	6	chain B and resid 234:316
3	1	6	chain C and resid 234:316
4	1	6	chain D and resid 234:316

NCS ensembles :

ID
1
2
3
4
5
6

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DNA polymerase accessory protein ... , 2 types, 5 molecules BCDEA

#1: Protein	DNA polymerase accessory protein 44 / Clamp loader large subunit / Protein Gp44 Mass: 36189.602 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Gene: 44, gp44 / Production host: Escherichia coli (E. coli) / Strain (production host): T7 Express / References: UniProt: P04526
#2: Protein	DNA polymerase accessory protein 62 / Clamp loader small subunit / Protein Gp62 Mass: 22941.299 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Gene: 62, gp62 / Production host: Escherichia coli (E. coli) / Strain (production host): T7 Express / References: UniProt: P04527

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Protein , 1 types, 3 molecules GHF

#3: Protein	DNA polymerase processivity component / DNA polymerase accessory protein 45 / Gp45 Mass: 25162.592 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Gene: 45, gp45 / Production host: Escherichia coli (E. coli) / Strain (production host): T7 Express / References: UniProt: P04525

#3: Protein

DNA polymerase processivity component / DNA polymerase accessory protein 45 / Gp45

Mass: 25162.592 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Enterobacteria phage T4 (virus) / Gene: 45, gp45 / Production host:

Escherichia coli (E. coli) / Strain (production host): T7 Express / References: UniProt: P04525

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DNA chain , 2 types, 2 molecules IJ

#4: DNA chain	Template DNA strand Mass: 6117.943 Da / Num. of mol.: 1 / Source method: obtained synthetically
#5: DNA chain	Primer DNA strand Mass: 3013.995 Da / Num. of mol.: 1 / Source method: obtained synthetically

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Non-polymers , 3 types, 6 molecules

#6: Chemical	ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate Mass: 427.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₀P₂ / Comment: ADP, energy-carrying molecule*YM
#7: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg
#8: Chemical	ChemComp-08T / [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium Mass: 492.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₀H₁₄BeF₃N₅O₁₀P₂

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.49 Å³/Da / Density % sol: 50.68 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 10% PEG4k, 0.1M MES pH 6.5, 20mM NaCl, 10mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å
Detector	Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jan 1, 2009
Radiation	Monochromator: Double crystal, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 3.2→50 Å / Num. all: 44596 / Num. obs: 44464 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 23.7 % / R_sym value: 0.123 / Net I/σ(I): 18.9
Reflection shell	Resolution: 3.2→3.32 Å / Redundancy: 17.2 % / Mean I/σ(I) obs: 6 / R_sym value: 0.483 / % possible all: 97.8

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Processing

Software

Name	Version	Classification
HKL-2000		data collection
PHASER		phasing
PHENIX	(phenix.refine: 1.7.1_743)	refinement
HKL-2000		data reduction
HKL-2000		data scaling

Refinement

Method to determine structure: molecular replacementSAD / Resolution: 3.2→47.646 Å / SU ML: 0.94 / σ(F): 1 / Phase error: 30.81 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.3051	2913	7.13 %	random
R_work	0.2392	-	-	-
obs	0.2439	40848	96.16 %	-
all	-	42479	-	-

Solvent computation

Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / B_sol: 44.859 Å² / k_sol: 0.294 e/Å³

Displacement parameters

	B_aniso -1	B_aniso -2	B_aniso -3
1-	25.0351 Å²	-0 Å²	-0 Å²
2-	-	-8.4104 Å²	-0 Å²
3-	-	-	-16.6247 Å²

Refinement step

Cycle: LAST / Resolution: 3.2→47.646 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	16331	325	92	0	16748

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.011	17060
X-RAY DIFFRACTION	f_angle_d	1.568	23119
X-RAY DIFFRACTION	f_dihedral_angle_d	18.146	6372
X-RAY DIFFRACTION	f_chiral_restr	0.101	2649
X-RAY DIFFRACTION	f_plane_restr	0.006	2894

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Type	Rms dev position (Å)
1	1	F	762	X-RAY DIFFRACTION	POSITIONAL
1	2	G	762	X-RAY DIFFRACTION	POSITIONAL	0.067
1	3	H	762	X-RAY DIFFRACTION	POSITIONAL	0.074
2	1	F	923	X-RAY DIFFRACTION	POSITIONAL
2	2	G	923	X-RAY DIFFRACTION	POSITIONAL	0.069
2	3	H	923	X-RAY DIFFRACTION	POSITIONAL	0.088
3	1	B	1190	X-RAY DIFFRACTION	POSITIONAL
3	2	C	1190	X-RAY DIFFRACTION	POSITIONAL	0.094
4	1	B	395	X-RAY DIFFRACTION	POSITIONAL
4	2	C	395	X-RAY DIFFRACTION	POSITIONAL	0.065
5	1	A	72	X-RAY DIFFRACTION	POSITIONAL
5	2	B	72	X-RAY DIFFRACTION	POSITIONAL	0.088
5	3	C	72	X-RAY DIFFRACTION	POSITIONAL	0.162
6	1	A	668	X-RAY DIFFRACTION	POSITIONAL
6	2	B	668	X-RAY DIFFRACTION	POSITIONAL	0.053
6	3	C	668	X-RAY DIFFRACTION	POSITIONAL	0.056
6	4	D	660	X-RAY DIFFRACTION	POSITIONAL	0.055

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.2-3.2525	0.3802	130	0.3114	1618	X-RAY DIFFRACTION	88
3.2525-3.3085	0.3686	137	0.317	1704	X-RAY DIFFRACTION	92
3.3085-3.3687	0.375	149	0.3111	1674	X-RAY DIFFRACTION	93
3.3687-3.4335	0.3533	130	0.3041	1766	X-RAY DIFFRACTION	93
3.4335-3.5035	0.3415	114	0.2876	1722	X-RAY DIFFRACTION	94
3.5035-3.5797	0.3627	149	0.2903	1770	X-RAY DIFFRACTION	96
3.5797-3.6629	0.3341	141	0.2852	1749	X-RAY DIFFRACTION	96
3.6629-3.7545	0.2975	126	0.2725	1796	X-RAY DIFFRACTION	95
3.7545-3.856	0.3157	140	0.2583	1759	X-RAY DIFFRACTION	96
3.856-3.9694	0.3183	147	0.2445	1797	X-RAY DIFFRACTION	96
3.9694-4.0974	0.278	137	0.2438	1807	X-RAY DIFFRACTION	97
4.0974-4.2438	0.3156	140	0.2286	1783	X-RAY DIFFRACTION	96
4.2438-4.4136	0.3129	130	0.2072	1838	X-RAY DIFFRACTION	98
4.4136-4.6143	0.2656	160	0.2035	1822	X-RAY DIFFRACTION	98
4.6143-4.8573	0.2652	138	0.1929	1867	X-RAY DIFFRACTION	98
4.8573-5.1613	0.3042	131	0.2201	1845	X-RAY DIFFRACTION	98
5.1613-5.5593	0.3439	144	0.2618	1855	X-RAY DIFFRACTION	99
5.5593-6.1177	0.3735	150	0.263	1875	X-RAY DIFFRACTION	99
6.1177-7.0006	0.3294	135	0.2421	1912	X-RAY DIFFRACTION	99
7.0006-8.8109	0.2564	135	0.1792	1948	X-RAY DIFFRACTION	100
8.8109-47.6515	0.2555	150	0.2286	2028	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.0503	0.0472	0.9141	5.8956	-1.2702	2.6659	-0.187	0.1189	0.3595	-0.1622	-0.1306	0.3924	0.114	0.119	-0.0001	0.8385	0.0829	0.27	0.9356	0.118	1.2852	3.1735	1.9096	4.0109
2	1.2726	1.7617	0.4844	2.9175	-0.9547	2.5283	-0.2655	-0.8645	0.0376	0.165	0.4069	0.0407	-0.1658	-0.849	0.0001	1.2458	-0.0976	-0.083	1.3372	0.158	1.4734	4.6161	27.8034	1.1236
3	2.5018	-0.1378	0.848	3.3018	1.9979	3.7706	-0.2006	0.2727	0.2564	-0.1161	0.0401	-0.3507	0.2284	0.032	-0.0001	1.0507	0.118	-0.0526	1.1303	-0.1358	1.1715	-3.5406	23.1566	35.3209
4	2.9563	-0.289	-0.3418	5.4284	0.2496	3.9628	-0.0737	0.236	0.1479	-0.5743	0.1555	-0.5014	-0.12	0.283	0.0003	0.7052	0.1071	0.1089	0.7275	0.0393	0.8059	-26.7991	20.6606	13.3292
5	2.9149	0.2911	0.3449	2.7261	1.1758	2.5565	0.4468	-0.6729	0.3529	0.0464	-0.3669	-0.0945	-0.8361	-0.2865	-0.0002	1.1956	0.1415	-0.0895	0.8273	-0.0983	1.0854	-36.2631	37.4542	33.1165
6	2.7712	-0.3643	1.0281	2.5224	-1.4064	2.4964	-0.5124	-0.4071	0.7011	0.3232	0.5356	0.1186	0.0844	-0.2628	-0.0001	1.0466	0.3308	-0.1137	1.6006	-0.422	1.3122	-14.2176	34.1238	52.0998
7	1.6545	0.787	-2.4771	4.3497	-0.4801	7.1345	0.1644	0.0255	0.0713	0.0562	0.0587	0.2164	0.0256	-0.5725	-0.0009	0.5132	0.1373	0.0225	0.8486	0.0089	0.7251	-39.542	12.3044	39.5827
8	3.3377	-0.6024	-0.9389	2.3308	2.6085	4.497	0.1486	-0.6868	-0.6886	1.0528	0.1577	-0.5145	0.0339	-0.46	0	1.1026	0.1537	-0.0345	1.1053	-0.0576	0.9429	-40.5831	13.2504	67.0077
9	0.847	0.0527	0.2024	0.6807	0.3498	1.4974	-0.2857	-0.3438	0.3724	0.6604	-0.1806	-0.0101	-0.0636	-0.716	0.0004	1.8223	0.3829	-0.0824	2.1104	-0.2275	1.2297	-9.6185	21.7057	70.8237
10	0.7405	-2.3088	0.3879	6.2425	-2.0027	5.017	0.2082	-0.1721	-0.247	0.1059	-0.4836	-0.3243	0.4533	-0.0021	0.0001	0.9686	-0.0895	-0.1237	0.9021	0.04	0.884	-28.3084	-8.0484	58.511
11	0.5388	-1.3132	-0.0039	1.0046	0.0136	0.3294	0.1632	-0.2873	-0.0834	1.2455	0.153	-0.5476	-0.1366	-0.246	-0.0002	1.7224	-0.0982	-0.3728	1.2272	-0.0887	1.3484	-14.9961	-18.1547	78.4353
12	1.8329	-1.4678	-0.9474	1.7787	-0.501	1.6627	-0.7239	-1.0258	-0.3491	0.7514	0.6475	0.1585	0.989	0.3418	0.0001	2.1466	0.5882	0.0025	1.9099	0.0335	1.3371	3.6	3.8574	63.6497
13	3.0389	-0.1347	0.0385	1.6258	-1.4797	1.2947	0.3968	0.1772	-0.3092	-0.0578	0.0878	0.0016	-0.3934	0.0654	0.0001	1.0526	0.0374	0.1513	0.8799	0.223	1.1469	1.0711	-20.8571	12.83
14	1.1987	-0.4137	-0.3847	1.4525	0.6813	0.7616	-0.2151	0.1247	-0.4722	0.3938	0.1722	-0.7128	0.0792	-0.0056	0	1.4541	0.2305	0.121	1.3169	-0.0858	1.232	7.7951	3.2784	39.395
15	1.7768	-0.3428	-0.3293	-0.1182	0.1492	1.0396	0.239	0.5413	-0.4264	-0.5672	0.462	-0.1654	0.0641	0.4099	-0.0005	1.5899	-0.0002	-0.2725	1.3473	-0.0071	1.7177	-10.9409	-23.473	48.5746
16	2.2892	-1.027	-2.3943	0.487	0.2073	4.5664	0.0567	-0.0322	0.329	0.1039	-0.0366	0.5344	-0.2842	-0.8795	-0.0001	0.7422	0.1157	-0.0044	1.0977	0.0559	0.8754	-50.9392	-1.3898	11.4926
17	4.285	-1.4065	-0.1029	5.2256	0.097	3.8843	0.089	0.3394	0.1497	-0.6272	-0.1235	-0.5427	-0.0232	-0.0289	-0.0001	1.0078	0.0878	0.2559	0.7916	0.0997	0.8053	-31.4764	-0.1629	-10.6216
18	4.2003	-1.8084	-0.6656	4.5519	-0.2921	2.1056	-0.3129	-0.5242	-0.2202	1.101	-0.1747	-0.1781	0.8556	0.3429	-0.0001	1.1652	-0.2023	0.0298	1.1255	0.1793	0.9	-31.5843	-37.6826	35.2973
19	4.4737	-0.7707	1.1405	5.1729	-0.4169	3.5003	-0.1854	-0.0653	-0.165	0.8339	0.0765	0.6245	0.0629	-0.0836	-0.0004	0.8858	-0.2204	0.149	1.0996	0.0533	0.9033	-52.2695	-17.0854	37.4079
20	2.2058	-1.5125	-0.0708	5.5417	-0.9405	2.8178	1.0725	0.5109	0.3198	-0.9373	-0.5927	-0.69	0.1741	0.4544	0.0003	1.0449	0.1411	0.165	0.8749	-0.0341	1.0558	-14.0341	-25.4949	-7.6031
21	4.3667	-0.6368	0.5915	2.1325	0.1092	4.9062	0.2304	-0.2691	-0.6914	-0.0748	-0.1576	-0.0105	0.7424	0.5227	0.0004	1.1307	0.1267	0.0273	0.97	0.2088	1.1806	-10.9648	-44.1451	14.4007
22	0.8459	-0.4614	-0.1634	0.1804	0.192	0.7142	0.1464	-2.6795	-0.7329	0.5984	-0.2769	0.2067	1.3989	0.7079	0.002	1.2422	0.1367	0.2825	1.6347	-0.1139	1.6172	-13.4035	-1.0848	28.9066
23	0.7589	-0.3836	-0.0717	0.8588	-0.3341	0.0933	-0.0861	-0.0741	0.0884	0.0544	-0.0502	-0.5922	2.2802	0.6096	0.0015	1.0927	0.3546	-0.1051	1.3749	-0.0419	0.9968	-17.6616	-1.0542	29.8358

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN B AND RESID 2:159 )	B	2 - 159
2	X-RAY DIFFRACTION	2	( CHAIN B AND RESID 160:187 )	B	160 - 187
3	X-RAY DIFFRACTION	3	( CHAIN B AND RESID 234:318 )	B	234 - 318
4	X-RAY DIFFRACTION	4	( CHAIN C AND RESID 2:159 )	C	2 - 159
5	X-RAY DIFFRACTION	5	( CHAIN C AND RESID 160:221 )	C	160 - 221
6	X-RAY DIFFRACTION	6	( CHAIN C AND RESID 234:318 )	C	234 - 318
7	X-RAY DIFFRACTION	7	( CHAIN D AND RESID 1:159 )	D - C	1 - 159
8	X-RAY DIFFRACTION	8	( CHAIN D AND RESID 160:232 )	D - C	160 - 232
9	X-RAY DIFFRACTION	9	( CHAIN D AND RESID 233:318 )	D - C	233 - 318
10	X-RAY DIFFRACTION	10	( CHAIN E AND RESID 3:159 )	E	3 - 159
11	X-RAY DIFFRACTION	11	( CHAIN E AND RESID 160:213 )	E	160 - 213
12	X-RAY DIFFRACTION	12	( CHAIN E AND RESID 233:315 )	E	233 - 315
13	X-RAY DIFFRACTION	13	( CHAIN A AND RESID 3:37 )	A	3 - 37
14	X-RAY DIFFRACTION	14	( CHAIN A AND RESID 38:108 )	A	38 - 108
15	X-RAY DIFFRACTION	15	( CHAIN A AND RESID 117:187 )	A	117 - 187
16	X-RAY DIFFRACTION	16	( CHAIN G AND RESID 1001:1106 )	G	1001 - 1106
17	X-RAY DIFFRACTION	17	( CHAIN G AND RESID 1107:1228 )	G	1107 - 1228
18	X-RAY DIFFRACTION	18	( CHAIN H AND RESID 2001:2106 )	H	2001 - 2106
19	X-RAY DIFFRACTION	19	( CHAIN H AND RESID 2107:2228 )	H	2107 - 2228
20	X-RAY DIFFRACTION	20	( CHAIN F AND RESID 3001:3106 )	F	3001 - 3106
21	X-RAY DIFFRACTION	21	( CHAIN F AND RESID 3107:3222 )	F	3107 - 3222
22	X-RAY DIFFRACTION	22	chain I
23	X-RAY DIFFRACTION	23	chain J

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