+Open data
-Basic information
Entry | Database: PDB / ID: 2k9m | ||||||
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Title | Structure of the Core Binding Domain of sigma54 | ||||||
Components | RNA polymerase sigma factor RpoN | ||||||
Keywords | TRANSCRIPTION / Core Binding Domain | ||||||
Function / homology | Function and homology information DNA-binding transcription activator activity / sigma factor activity / nucleotidyltransferase activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / DNA binding Similarity search - Function | ||||||
Biological species | Aquifex aeolicus (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Hong, E. / Wemmer, D. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2009 Title: Structure of the RNA polymerase core-binding domain of sigma(54) reveals a likely conformational fracture point Authors: Hong, E. / Doucleff, M. / Wemmer, D.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2k9m.cif.gz | 831.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2k9m.ent.gz | 725.5 KB | Display | PDB format |
PDBx/mmJSON format | 2k9m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k9/2k9m ftp://data.pdbj.org/pub/pdb/validation_reports/k9/2k9m | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15222.607 Da / Num. of mol.: 1 / Fragment: sequence database residues 69-198 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aquifex aeolicus (bacteria) / Gene: rpoN / Production host: Escherichia coli (E. coli) / References: UniProt: O66858 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 / pH: 7 / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |