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- PDB-2k44: Solution structure of a K+-channel voltage-sensor paddle domain -

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Basic information

Entry
Database: PDB / ID: 2k44
TitleSolution structure of a K+-channel voltage-sensor paddle domain
ComponentsK+-channel voltage-sensor paddle domain of Calcium-activated potassium channel subunit alpha-1
KeywordsMEMBRANE PROTEIN / potassium channel / voltage-sensor / membrane / micelle / solution structure
Function / homology
Function and homology information


Acetylcholine inhibits contraction of outer hair cells / micturition / Ca2+ activated K+ channels / large conductance calcium-activated potassium channel activity / response to carbon monoxide / calcium-activated potassium channel activity / negative regulation of cell volume / intracellular potassium ion homeostasis / smooth muscle contraction involved in micturition / Sensory processing of sound by inner hair cells of the cochlea ...Acetylcholine inhibits contraction of outer hair cells / micturition / Ca2+ activated K+ channels / large conductance calcium-activated potassium channel activity / response to carbon monoxide / calcium-activated potassium channel activity / negative regulation of cell volume / intracellular potassium ion homeostasis / smooth muscle contraction involved in micturition / Sensory processing of sound by inner hair cells of the cochlea / response to osmotic stress / voltage-gated potassium channel activity / cGMP effects / voltage-gated potassium channel complex / potassium ion transmembrane transport / regulation of membrane potential / caveola / potassium ion transport / response to calcium ion / vasodilation / actin binding / postsynaptic membrane / response to hypoxia / positive regulation of apoptotic process / apical plasma membrane / membrane / identical protein binding / metal ion binding / plasma membrane
Similarity search - Function
: / Ca2+-activated K+ channel Slowpoke, TrkA_C like domain / : / Calcium-activated potassium channel BK, alpha subunit / Calcium-activated BK potassium channel alpha subunit / Ion transport domain / Ion transport protein / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Calcium-activated potassium channel subunit alpha-1
Similarity search - Component
MethodSOLUTION NMR / torsion angle dynamics
Model detailsNMR solution structure of a putative S3b-S4 domain of HsapBK(233-260) in DPC micelles
AuthorsUnnerstale, S. / Lind, J. / Papadopoulos, E. / Maler, L.
CitationJournal: Biochemistry / Year: 2009
Title: Solution structure of the HsapBK K+-channel voltage-sensor paddle sequence
Authors: Unnerstale, S. / Lind, J. / Papadopoulos, E. / Maler, L.
History
DepositionMay 28, 2008Deposition site: BMRB / Processing site: PDBJ
Revision 1.0Jun 2, 2009Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Mar 16, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name
Revision 1.3May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: K+-channel voltage-sensor paddle domain of Calcium-activated potassium channel subunit alpha-1


Theoretical massNumber of molelcules
Total (without water)3,3851
Polymers3,3851
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)30 / 100target function
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide K+-channel voltage-sensor paddle domain of Calcium-activated potassium channel subunit alpha-1 / HsapBK


Mass: 3385.016 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: synthetic peptide / References: UniProt: Q12791

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
Details: NMR solution structure of a putative S3b-S4 domain of HsapBK(233-260) in DPC micelles
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H TOCSY
1212D 1H-1H COSY
1312D 1H-1H NOESY

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Sample preparation

DetailsContents: 100mM [U-2H] 98% DPC, 50mM potassium chloride, 1mM HsapBK(233-260), 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
100 mMDPC[U-2H] 98%1
50 mMpotassium chloride1
1 mMHsapBK(233-260)1
Sample conditionsIonic strength: 0.05 / pH: 5.5 / Pressure: ambient / Temperature: 310 K

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NMR measurement

NMR spectrometerType: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz

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Processing

NMR software
NameVersionDeveloperClassification
Sparky3.113Goddardpeak picking
NMRDraw2.3Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Baxprocessing
CYANA2Guntert, Mumenthaler and Wuthrichdata analysis
CYANA2Guntert, Mumenthaler and Wuthrichstructure solution
CYANA2Guntert, Mumenthaler and Wuthrichrefinement
VNMRVariancollection
ProcheckNMRLaskowski and MacArthurrefinement
RefinementMethod: torsion angle dynamics / Software ordinal: 1 / Details: CYANA 2.0
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 30 / Representative conformer: 1

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