+Open data
-Basic information
Entry | Database: PDB / ID: 1uas | ||||||
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Title | Crystal structure of rice alpha-galactosidase | ||||||
Components | alpha-galactosidase | ||||||
Keywords | HYDROLASE / TIM-barrel / beta-alpha-barrel / greek key motif | ||||||
Function / homology | Function and homology information leaf morphogenesis / raffinose alpha-galactosidase activity / positive regulation of flower development / plant-type cell wall / alpha-galactosidase / response to fungus / apoplast / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | Oryza sativa (Asian cultivated rice) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 1.5 Å | ||||||
Authors | Fujimoto, Z. / Kaneko, S. / Momma, M. / Kobayashi, H. / Mizuno, H. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Crystal structure of rice alpha-galactosidase complexed with D-galactose Authors: Fujimoto, Z. / Kaneko, S. / Momma, M. / Kobayashi, H. / Mizuno, H. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2002 Title: Crystallization and preliminary X-ray crystallographic studies of rice alpha-galactosidase Authors: Fujimoto, Z. / Kobayashi, O. / Kaneko, S. / Momma, M. / Kobayashi, H. / Mizuno, H. #2: Journal: PHYTOCHEMISTRY / Year: 2002 Title: alpha-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells Authors: Kim, W. / Kobayashi, O. / Kaneko, S. / Sakakibara, Y. / Park, G. / Kusakabe, I. / Tanaka, H. / Kobayashi, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uas.cif.gz | 100.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uas.ent.gz | 73.8 KB | Display | PDB format |
PDBx/mmJSON format | 1uas.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ua/1uas ftp://data.pdbj.org/pub/pdb/validation_reports/ua/1uas | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / Sugars , 2 types, 2 molecules A
#1: Protein | Mass: 39989.273 Da / Num. of mol.: 1 / Fragment: resiudes 1-362 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Oryza sativa (Asian cultivated rice) / Plasmid: pET-28 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9FXT4, alpha-galactosidase |
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#2: Sugar | ChemComp-GLA / |
-Non-polymers , 4 types, 647 molecules
#3: Chemical | ChemComp-SO4 / | ||
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#4: Chemical | ChemComp-PT / | ||
#5: Chemical | ChemComp-GOL / #6: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 37.87 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 2-propanol, ammonium sulfate, acetate, D-galactose, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jan 31, 2002 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→100 Å / Num. all: 63606 / Num. obs: 63606 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.4 % / Biso Wilson estimate: 12.9 Å2 / Rmerge(I) obs: 0.055 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 1.5→1.55 Å / Rmerge(I) obs: 0.33 / Num. unique all: 6311 / % possible all: 99.9 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. obs: 61397 |
Reflection shell | *PLUS % possible obs: 99.9 % / Num. unique obs: 6311 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.5→29.86 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 197438.36 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 60.2438 Å2 / ksol: 0.362743 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.5→29.86 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.59 Å / Rfactor Rfree error: 0.007 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 30 Å / % reflection Rfree: 10 % / Rfactor Rwork: 0.16 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Lowest resolution: 1.55 Å / Rfactor Rfree: 0.216 / Rfactor Rwork: 0.214 / Num. reflection obs: 5895 |