+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1gol | ||||||
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タイトル | COORDINATES OF RAT MAP KINASE ERK2 WITH AN ARGININE MUTATION AT POSITION 52 | ||||||
要素 | EXTRACELLULAR REGULATED KINASE 2 | ||||||
キーワード | TRANSFERASE (転移酵素) / SERINE/THREONINE-PROTEIN KINASE / ATP-BINDING / CELL CYCLE (細胞周期) / PHOSPHORYLATION (リン酸化) | ||||||
機能・相同性 | 機能・相同性情報 phospho-PLA2 pathway / RAF-independent MAPK1/3 activation / MAPK1 (ERK2) activation / Signaling by NODAL / Frs2-mediated activation / ERK/MAPK targets / ERKs are inactivated / Activation of the AP-1 family of transcription factors / RHO GTPases Activate WASPs and WAVEs / IFNG signaling activates MAPKs ...phospho-PLA2 pathway / RAF-independent MAPK1/3 activation / MAPK1 (ERK2) activation / Signaling by NODAL / Frs2-mediated activation / ERK/MAPK targets / ERKs are inactivated / Activation of the AP-1 family of transcription factors / RHO GTPases Activate WASPs and WAVEs / IFNG signaling activates MAPKs / Negative feedback regulation of MAPK pathway / Gastrin-CREB signalling pathway via PKC and MAPK / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Golgi Cisternae Pericentriolar Stack Reorganization / Regulation of actin dynamics for phagocytic cup formation / Estrogen-stimulated signaling through PRKCZ / Growth hormone receptor signaling / Spry regulation of FGF signaling / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / Oxidative Stress Induced Senescence / Senescence-Associated Secretory Phenotype (SASP) / Oncogene Induced Senescence / Signaling by Activin / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Signal attenuation / NCAM signaling for neurite out-growth / Negative regulation of FGFR1 signaling / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / Regulation of the apoptosome activity / Signal transduction by L1 / Negative regulation of FGFR2 signaling / RHO GTPases Activate NADPH Oxidases / Negative regulation of MAPK pathway / Interferon gamma signaling / FCERI mediated MAPK activation / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Regulation of HSF1-mediated heat shock response / MAP2K and MAPK activation / diadenosine tetraphosphate biosynthetic process / Recycling pathway of L1 / neural crest cell development / cardiac neural crest cell development involved in heart development / caveolin-mediated endocytosis / cytosine metabolic process / response to epidermal growth factor / mitogen-activated protein kinase kinase kinase binding / regulation of cellular pH / positive regulation of macrophage proliferation / outer ear morphogenesis / Thrombin signalling through proteinase activated receptors (PARs) / RAF/MAP kinase cascade / regulation of Golgi inheritance / ERBB signaling pathway / labyrinthine layer blood vessel development / Neutrophil degranulation / mammary gland epithelial cell proliferation / trachea formation / regulation of early endosome to late endosome transport / : / regulation of stress-activated MAPK cascade / positive regulation of macrophage chemotaxis / lung morphogenesis / ERBB2-ERBB3 signaling pathway / response to exogenous dsRNA / regulation of cytoskeleton organization / face development / androgen receptor signaling pathway / 仮足 / progesterone receptor signaling pathway / negative regulation of cell differentiation / Bergmann glial cell differentiation / positive regulation of telomere capping / thyroid gland development / decidualization / steroid hormone mediated signaling pathway / MAP kinase activity / regulation of ossification / 分裂促進因子活性化タンパク質キナーゼ / phosphatase binding / Schwann cell development / stress-activated MAPK cascade / lipopolysaccharide-mediated signaling pathway / positive regulation of cardiac muscle cell proliferation / sensory perception of pain / cellular response to cadmium ion / positive regulation of telomere maintenance via telomerase / ERK1 and ERK2 cascade / cellular response to amino acid starvation / 髄鞘 / dendrite cytoplasm / phosphotyrosine residue binding / RNA polymerase II CTD heptapeptide repeat kinase activity / insulin-like growth factor receptor signaling pathway / thymus development / positive regulation of peptidyl-threonine phosphorylation / カベオラ / positive regulation of translation / long-term synaptic potentiation / animal organ morphogenesis 類似検索 - 分子機能 | ||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||
手法 | X線回折 / 解像度: 2.8 Å | ||||||
データ登録者 | Harkins, P.C. / Zhang, F. / Goldsmith, E.J. | ||||||
引用 | ジャーナル: Biochemistry / 年: 1996 タイトル: Mutation of position 52 in ERK2 creates a nonproductive binding mode for adenosine 5'-triphosphate. 著者: Robinson, M.J. / Harkins, P.C. / Zhang, J. / Baer, R. / Haycock, J.W. / Cobb, M.H. / Goldsmith, E.J. #1: ジャーナル: Structure / 年: 1995 タイトル: Activity of the Map Kinase Erk2 is Controlled by a Flexible Surface Loop 著者: Zhang, J. / Zhang, F. / Ebert, D. / Cobb, M.H. / Goldsmith, E.J. #2: ジャーナル: Nature / 年: 1994 タイトル: Atomic Structure of the Map Kinase Erk2 at 2.3 A Resolution 著者: Zhang, F. / Strand, A. / Robbins, D. / Cobb, M.H. / Goldsmith, E.J. #3: ジャーナル: J.Mol.Biol. / 年: 1993 タイトル: Crystallization and Preliminary X-Ray Studies of Extracellular Signal-Regulated Kinase-2/Map Kinase with an Incorporated His-Tag 著者: Zhang, F. / Robbins, D.J. / Cobb, M.H. / Goldsmith, E.J. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1gol.cif.gz | 88.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1gol.ent.gz | 66 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1gol.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/go/1gol ftp://data.pdbj.org/pub/pdb/validation_reports/go/1gol | HTTPS FTP |
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-関連構造データ
類似構造データ |
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-リンク
-集合体
登録構造単位 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 42187.449 Da / 分子数: 1 / 変異: K52R / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 細胞株: BL21 / 遺伝子: ERK2 / プラスミド: BL21 / 生物種 (発現宿主): Escherichia coli / 遺伝子 (発現宿主): ERK2 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21 (DE3) 参照: UniProt: P63086, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) |
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#2: 化合物 | ChemComp-MG / |
#3: 化合物 | ChemComp-ATP / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 2.41 Å3/Da / 溶媒含有率: 48.87 % | ||||||||||||||||||||
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結晶化 | *PLUS 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射光源 | 波長: 1.5418 |
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検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1995年7月7日 / 詳細: MIRRORS |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 冗長度: 4 % / Rmerge(I) obs: 0.056 |
-解析
ソフトウェア |
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精密化 | 最高解像度: 2.8 Å | ||||||||||||
精密化ステップ | サイクル: LAST / 最高解像度: 2.8 Å
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