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Yorodumi- PDB-1a68: CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POT... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a68 | ||||||
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Title | CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL | ||||||
Components | POTASSIUM CHANNEL KV1.1 | ||||||
Keywords | POTASSIUM CHANNELS / TETRAMERIZATION DOMAIN / APLYSIA KV1.1 | ||||||
Function / homology | Function and homology information monoatomic ion-gated channel activity / voltage-gated potassium channel activity / voltage-gated potassium channel complex / protein homooligomerization Similarity search - Function | ||||||
Biological species | Aplysia californica (California sea hare) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Kreusch, A. / Pfaffinger, P.J. / Stevens, C.F. / Choe, S. | ||||||
Citation | Journal: Nature / Year: 1998 Title: Crystal structure of the tetramerization domain of the Shaker potassium channel. Authors: Kreusch, A. / Pfaffinger, P.J. / Stevens, C.F. / Choe, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a68.cif.gz | 29.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a68.ent.gz | 19.8 KB | Display | PDB format |
PDBx/mmJSON format | 1a68.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/1a68 ftp://data.pdbj.org/pub/pdb/validation_reports/a6/1a68 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11493.914 Da / Num. of mol.: 1 / Fragment: TETRAMERIZATION DOMAIN / Mutation: INSERTED MET AT N-TERMINUS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aplysia californica (California sea hare) Tissue: CENTRAL NERVOUS SYSTEM / Cell line: BL21 / Cellular location: CYTOPLASM / Plasmid: PET20B / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: Q16968 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 7-8% PEG8000, 300 MM NACL, 200MM MGCL2, 20 MM TRIS, PH 8.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL1-5 / Wavelength: 0.9799 |
Detector | Type: SIEMENS / Detector: CCD AREA DETECTOR / Date: Aug 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9799 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→25 Å / Num. obs: 7712 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 10.9 % / Biso Wilson estimate: 18.9 Å2 / Rsym value: 0.063 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 1.8→1.91 Å / Mean I/σ(I) obs: 3.5 / Rsym value: 0.035 / % possible all: 97.2 |
Reflection | *PLUS Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS % possible obs: 97.2 % / Rmerge(I) obs: 0.035 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→10 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 26.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.038 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.334 |