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Yorodumi- EMDB-43431: Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1,... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-43431 | |||||||||||||||
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Title | Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1, and RDE-4 bound to two dsRNA | |||||||||||||||
Map data | ||||||||||||||||
Sample |
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Keywords | helicase / RLR / RIG-I-like Receptor / ATPase / dsRNA / ANTIVIRAL PROTEIN-RNA complex / ANTIVIRAL PROTEIN | |||||||||||||||
Biological species | Caenorhabditis elegans (invertebrata) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.6 Å | |||||||||||||||
Authors | Consalvo CD / Donelick HM / Shen PS / Bass BL | |||||||||||||||
Funding support | United States, 4 items
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Citation | Journal: To Be Published Title: C. elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA Authors: Consalvo CD / Aderounmu AM / Donelick HM / Aruscavage PJ / Eckert D / Shen PS / Bass BL | |||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_43431.map.gz | 51.3 MB | EMDB map data format | |
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Header (meta data) | emd-43431-v30.xml emd-43431.xml | 12.6 KB 12.6 KB | Display Display | EMDB header |
Images | emd_43431.png | 13.1 KB | ||
Filedesc metadata | emd-43431.cif.gz | 3.8 KB | ||
Others | emd_43431_half_map_1.map.gz emd_43431_half_map_2.map.gz | 95.4 MB 95.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-43431 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43431 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_43431.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.576 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_43431_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_43431_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1,...
Entire | Name: Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1, and RDE-4 bound to two dsRNA |
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Components |
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-Supramolecule #1: Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1,...
Supramolecule | Name: Cryo-EM structure of C. elegans antiviral complex Dicer-1, DRH-1, and RDE-4 bound to two dsRNA type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.1 µm |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 38.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER / Details: Ab Initio |
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Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 7.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 26879 |