[English] 日本語
Yorodumi- EMDB-37186: Jackbean Urease (Reprocessing of EMPIAR-10549 for comparison to P... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37186 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Jackbean Urease (Reprocessing of EMPIAR-10549 for comparison to PASR processing) | |||||||||
Map data | Post-processed map Jackbean Urease (Reprocessing of EMPIAR-10549) | |||||||||
Sample |
| |||||||||
Keywords | PASR / post-acquisition super resolution / jackbean urase / EMPIAR dataset / HYDROLASE | |||||||||
Biological species | Canavalia ensiformis (jack bean) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.07 Å | |||||||||
Authors | Burton-Smith RN / Murata K | |||||||||
Funding support | Japan, 1 items
| |||||||||
Citation | Journal: To Be Published Title: Jackbean Urease (Reprocessing of EMPIAR-10549 for comparison to PASR processing) Authors: Burton-Smith RN / Murata K | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_37186.map.gz | 49.4 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-37186-v30.xml emd-37186.xml | 11.3 KB 11.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37186_fsc.xml | 8.5 KB | Display | FSC data file |
Images | emd_37186.png | 119.2 KB | ||
Filedesc metadata | emd-37186.cif.gz | 3.6 KB | ||
Others | emd_37186_half_map_1.map.gz emd_37186_half_map_2.map.gz | 41.2 MB 41.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37186 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37186 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_37186.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Post-processed map Jackbean Urease (Reprocessing of EMPIAR-10549) | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.05 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: Half-map 1 Jackbean Urease (Reprocessing of EMPIAR-10549)
File | emd_37186_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Half-map 1 Jackbean Urease (Reprocessing of EMPIAR-10549) | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: Half-map 2 Jackbean Urease (Reprocessing of EMPIAR-10549)
File | emd_37186_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Half-map 2 Jackbean Urease (Reprocessing of EMPIAR-10549) | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : Jackbean urease
Entire | Name: Jackbean urease |
---|---|
Components |
|
-Supramolecule #1: Jackbean urease
Supramolecule | Name: Jackbean urease / type: complex / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: Canavalia ensiformis (jack bean) |
Molecular weight | Theoretical: 550 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1 mg/mL |
---|---|
Buffer | pH: 7.4 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TECNAI ARCTICA |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 39.93 e/Å2 |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |