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Yorodumi- EMDB-34662: TOM-TIM23 supercomplex with a GFP and DHFR containing substrate -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-34662 | |||||||||
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Title | TOM-TIM23 supercomplex with a GFP and DHFR containing substrate | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Mitochondrial protein import / TOM40 / TIM23 / PROTEIN TRANSPORT | |||||||||
Biological species | Saccharomyces cerevisiae S288C (yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 9.99 Å | |||||||||
Authors | Zhou XY / Yang YQ / Wang GP / Wang SS | |||||||||
Funding support | China, 1 items
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Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2023 Title: Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex Authors: Zhou XY / Yang YQ / Wang GP / Wang SS / Sun DJ / Ou XM / Lian Y / Li L | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_34662.map.gz | 7.9 MB | EMDB map data format | |
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Header (meta data) | emd-34662-v30.xml emd-34662.xml | 12.9 KB 12.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_34662_fsc.xml | 10.1 KB | Display | FSC data file |
Images | emd_34662.png | 20.4 KB | ||
Others | emd_34662_half_map_1.map.gz emd_34662_half_map_2.map.gz | 65.2 MB 65.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-34662 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34662 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_34662.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34662_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34662_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : TOM-TIM23 supercomplex with a GFP and DHFR containing substrate
Entire | Name: TOM-TIM23 supercomplex with a GFP and DHFR containing substrate |
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Components |
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-Supramolecule #1: TOM-TIM23 supercomplex with a GFP and DHFR containing substrate
Supramolecule | Name: TOM-TIM23 supercomplex with a GFP and DHFR containing substrate type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Saccharomyces cerevisiae S288C (yeast) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.2 µm / Nominal defocus min: 1.0 µm |
Specialist optics | Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |