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- EMDB-33501: Structure of photosynthetic LH1-RC super-complex of Rhodopila glo... -

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Basic information

Entry
Database: EMDB / ID: EMD-33501
TitleStructure of photosynthetic LH1-RC super-complex of Rhodopila globiformis
Map data
Sample
  • Complex: Photosynthetic LH1-RC complex from the purple phototrophic bacterium Rhodopila globiformis
    • Protein or peptide: x 7 types
  • Ligand: x 12 types
Function / homology
Function and homology information


organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / electron transfer activity / membrane => GO:0016020 / iron ion binding ...organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / electron transfer activity / membrane => GO:0016020 / iron ion binding / heme binding / metal ion binding / plasma membrane
Similarity search - Function
Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain ...Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain / Antenna complex, alpha/beta subunit / Light-harvesting complex / Antenna complex alpha/beta subunit / Bacterial photosynthetic reaction centre, H-chain, C-terminal / Photosynthetic reaction centre, M subunit / Photosynthetic reaction centre, H subunit, N-terminal / Photosynthetic reaction centre, H subunit, N-terminal domain superfamily / Photosynthetic reaction centre, H-chain N-terminal region / PRC-barrel domain / PRC-barrel domain / Photosynthetic reaction centre, L subunit / PRC-barrel-like superfamily / Multiheme cytochrome superfamily / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature.
Similarity search - Domain/homology
Photosynthetic reaction center subunit H / Reaction center protein L chain / Light-harvesting protein / Photosynthetic reaction center cytochrome c subunit / Light-harvesting protein / Reaction center protein M chain
Similarity search - Component
Biological speciesRhodopila globiformis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.24 Å
AuthorsTani K / Kanno R / Kurosawa K / Takaichi S / Nagashima KVP / Hall M / Yu L-J / Kimura Y / Madigan MT / Mizoguchi A ...Tani K / Kanno R / Kurosawa K / Takaichi S / Nagashima KVP / Hall M / Yu L-J / Kimura Y / Madigan MT / Mizoguchi A / Humbel BM / Wang-Otomo Z-Y
Funding support Japan, 6 items
OrganizationGrant numberCountry
Japan Agency for Medical Research and Development (AMED)JP21am0101118 Japan
Japan Agency for Medical Research and Development (AMED)JP21am0101116 Japan
Japan Society for the Promotion of Science (JSPS)JP16H04174 Japan
Japan Society for the Promotion of Science (JSPS)JP18H05153 Japan
Japan Society for the Promotion of Science (JSPS)20H05086 Japan
Japan Society for the Promotion of Science (JSPS)20H02856 Japan
Citation
Journal: Commun Biol / Year: 2022
Title: An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins.
Authors: Kazutoshi Tani / Ryo Kanno / Keigo Kurosawa / Shinichi Takaichi / Kenji V P Nagashima / Malgorzata Hall / Long-Jiang Yu / Yukihiro Kimura / Michael T Madigan / Akira Mizoguchi / Bruno M ...Authors: Kazutoshi Tani / Ryo Kanno / Keigo Kurosawa / Shinichi Takaichi / Kenji V P Nagashima / Malgorzata Hall / Long-Jiang Yu / Yukihiro Kimura / Michael T Madigan / Akira Mizoguchi / Bruno M Humbel / Zheng-Yu Wang-Otomo /
Abstract: Rhodopila globiformis is the most acidophilic of anaerobic purple phototrophs, growing optimally in culture at pH 5. Here we present a cryo-EM structure of the light-harvesting 1-reaction center (LH1- ...Rhodopila globiformis is the most acidophilic of anaerobic purple phototrophs, growing optimally in culture at pH 5. Here we present a cryo-EM structure of the light-harvesting 1-reaction center (LH1-RC) complex from Rhodopila globiformis at 2.24 Å resolution. All purple bacterial cytochrome (Cyt, encoded by the gene pufC) subunit-associated RCs with known structures have their N-termini truncated. By contrast, the Rhodopila globiformis RC contains a full-length tetra-heme Cyt with its N-terminus embedded in the membrane forming an α-helix as the membrane anchor. Comparison of the N-terminal regions of the Cyt with PufX polypeptides widely distributed in Rhodobacter species reveals significant structural similarities, supporting a longstanding hypothesis that PufX is phylogenetically related to the N-terminus of the RC-bound Cyt subunit and that a common ancestor of phototrophic Proteobacteria contained a full-length tetra-heme Cyt subunit that evolved independently through partial deletions of its pufC gene. Eleven copies of a novel γ-like polypeptide were also identified in the bacteriochlorophyll a-containing Rhodopila globiformis LH1 complex; γ-polypeptides have previously been found only in the LH1 of bacteriochlorophyll b-containing species. These features are discussed in relation to their predicted functions of stabilizing the LH1 structure and regulating quinone transport under the warm acidic conditions.
#1: Journal: Commun Biol / Year: 2022
Title: An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins
Authors: Tani K / Kanno R / Kurosawa K / Takaichi S / Nagashima KVP / Hall M / Yu L-J / Kimura Y / Madigan MT / Mizoguchi A / Humbel BM / Wang-Otomo Z-Y
History
DepositionMay 30, 2022-
Header (metadata) releaseNov 16, 2022-
Map releaseNov 16, 2022-
UpdateNov 23, 2022-
Current statusNov 23, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_33501.png

Downloads & links

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Map

FileDownload / File: emd_33501.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.82 Å
Density
Contour LevelBy AUTHOR: 0.03
Minimum - Maximum-0.10491291 - 0.3155316
Average (Standard dev.)9.0753754e-05 (±0.0074678315)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 328.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map of odd number

Fileemd_33501_half_map_1.map
Annotationhalf map of odd number
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map of even number

Fileemd_33501_half_map_2.map
Annotationhalf map of even number
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Photosynthetic LH1-RC complex from the purple phototrophic bacter...

EntireName: Photosynthetic LH1-RC complex from the purple phototrophic bacterium Rhodopila globiformis
Components
  • Complex: Photosynthetic LH1-RC complex from the purple phototrophic bacterium Rhodopila globiformis
    • Protein or peptide: Photosynthetic reaction center cytochrome c subunit
    • Protein or peptide: Reaction center protein L chainPhotosynthetic reaction centre
    • Protein or peptide: Reaction center protein M chainPhotosynthetic reaction centre
    • Protein or peptide: Photosynthetic reaction center H subunit
    • Protein or peptide: Light-harvesting protein
    • Protein or peptide: Light-harvesting protein
    • Protein or peptide: Light-harvesting protein LH1 Gamma-like
  • Ligand: HEME C
  • Ligand: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
  • Ligand: BACTERIOCHLOROPHYLL ABacteriochlorophyll
  • Ligand: BACTERIOPHEOPHYTIN A
  • Ligand: UBIQUINONE-10Coenzyme Q10
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: FE (III) ION
  • Ligand: MENAQUINONE-9Vitamin K2
  • Ligand: (6~{E},8~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{E},24~{E},26~{E},28~{E})-2,31-dimethoxy-2,6,10,14,19,23,27,31-octamethyl-dotriaconta-6,8,10,12,14,16,18,20,22,24,26,28-dodecaen-5-one
  • Ligand: CARDIOLIPIN
  • Ligand: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
  • Ligand: water

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Supramolecule #1: Photosynthetic LH1-RC complex from the purple phototrophic bacter...

SupramoleculeName: Photosynthetic LH1-RC complex from the purple phototrophic bacterium Rhodopila globiformis
type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#7
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 400 KDa

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Macromolecule #1: Photosynthetic reaction center cytochrome c subunit

MacromoleculeName: Photosynthetic reaction center cytochrome c subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 37.945188 KDa
SequenceString: MNTGVQAALA AAAVAAVAVA GVVFGTFERP PIETVQRGAR GLAMSELYNP RFLAETRAEN VVPASLPRLP DVGLKAGEVY HNVQVLKDV SVGNFTRLMA SMTTWVAPQQ GCGYCHNTNN MASDAKYTKV VARRMIQMVQ HINQDWKVHV MANAPTGVVC Y TCHRGNPV ...String:
MNTGVQAALA AAAVAAVAVA GVVFGTFERP PIETVQRGAR GLAMSELYNP RFLAETRAEN VVPASLPRLP DVGLKAGEVY HNVQVLKDV SVGNFTRLMA SMTTWVAPQQ GCGYCHNTNN MASDAKYTKV VARRMIQMVQ HINQDWKVHV MANAPTGVVC Y TCHRGNPV PKNIWFNNPG PLQAGGYAEA EIGKNHPAPF ANNSSLPLDP FTPFLEHAEN IRVQATQALP GTDNSSIKQT YW TYALMAS FTQALGVNCT YCHDSRLWES WDMAPPQRVT AWYGIRMVRD LNNNFLDPLK TTFPDYRRGP LGDSPKVWCA TCH NGVYKP LFGKSMVTTF PELTKVSQ

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Macromolecule #2: Reaction center protein L chain

MacromoleculeName: Reaction center protein L chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 30.697746 KDa
SequenceString: MAMLSFEPKY RTRGGTLIGG DLFDFWVGPL WVGFFGVTAA FFAILGTLLI VWAAALGPTW NIWRINIAPP DISYGLAFAP LREGGLWQL ITVCACGAFV SWALRQVEIA RKLGMGLHIP FAFSFAILAY FTLVVFRPLL MGAWGYGFPY GILSHLDWVS N TGYQYLHF ...String:
MAMLSFEPKY RTRGGTLIGG DLFDFWVGPL WVGFFGVTAA FFAILGTLLI VWAAALGPTW NIWRINIAPP DISYGLAFAP LREGGLWQL ITVCACGAFV SWALRQVEIA RKLGMGLHIP FAFSFAILAY FTLVVFRPLL MGAWGYGFPY GILSHLDWVS N TGYQYLHF HYNPAHMIAI SFFFTNALAL ALHGSLILSA ANPPKGEVVK GAEQENGYFR DVIGYSIGTL GIHRLGVFLA VS AAFWSAV CIIISGPFWT RGWPEWWSWW LNLPMWSH

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Macromolecule #3: Reaction center protein M chain

MacromoleculeName: Reaction center protein M chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 36.345332 KDa
SequenceString: MPEFQNVFTR VQVKGPVHMG VPLPRGSWAR TGKPFHLYLL GLIGDAQIGP IYLGFSGVAS IIFGFIAIEI IGFNMLASVD WSVPEFFRQ FFWLALEPPA PKYGLGLAPL AEGGWWGMAG FFLTASILLW WVRMYRRARA LGLGTHTAWA FASAIFLYLS L GFIRPILM ...String:
MPEFQNVFTR VQVKGPVHMG VPLPRGSWAR TGKPFHLYLL GLIGDAQIGP IYLGFSGVAS IIFGFIAIEI IGFNMLASVD WSVPEFFRQ FFWLALEPPA PKYGLGLAPL AEGGWWGMAG FFLTASILLW WVRMYRRARA LGLGTHTAWA FASAIFLYLS L GFIRPILM GCWCEAPPFG IFPHLDWTAA FSLRYGNLFY NPFHMLSIAF LYGSAVLFAM HGGTVLATTR FGGEREVEQI TD RGTAGER AMLFWRWTMG FNATFESIHR WGWWFAVLVT LTGGIGILLT GTVVDNWFLW GVKHGIAAPW PNVFPHVVDP ALL ATGVGK

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Macromolecule #4: Photosynthetic reaction center H subunit

MacromoleculeName: Photosynthetic reaction center H subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 28.383367 KDa
SequenceString: MEIGAITQQI DAAQLVLYTF WLFFAGLIIY LRMEDKREGY PLVTEIPGKF LEGFPPMPAP KTFILTHNQG TVTVPRAVPR AEIEYKAEP CAAWPGAPHE PVGPNKMLSG AGPSGYALRF DTPEPTFDTG VPRMAPMRVA TDHVFDEDGP NPIGYDLVGF D GIVAGKIT ...String:
MEIGAITQQI DAAQLVLYTF WLFFAGLIIY LRMEDKREGY PLVTEIPGKF LEGFPPMPAP KTFILTHNQG TVTVPRAVPR AEIEYKAEP CAAWPGAPHE PVGPNKMLSG AGPSGYALRF DTPEPTFDTG VPRMAPMRVA TDHVFDEDGP NPIGYDLVGF D GIVAGKIT DAWVDREESL VRYLEAKLTN DKSILVPMPL SRVKDSTGQV LLASLKGEQV LEAPTLANPD QVTLREEDRI AA YFASGHL YATQARQESI L

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Macromolecule #5: Light-harvesting protein

MacromoleculeName: Light-harvesting protein / type: protein_or_peptide / ID: 5 / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 7.048523 KDa
SequenceString:
(FME)WRMWLLFDP RRILVALGVF LFVLALLIHF ILLSTDRFNW LDGPHRGAVA AQMAPLPAPV K

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Macromolecule #6: Light-harvesting protein

MacromoleculeName: Light-harvesting protein / type: protein_or_peptide / ID: 6 / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 7.877075 KDa
SequenceString:
MTPGGPSITG LTEAEAKEFH GIFITSFIVF TVIAIVAHLL AWQWRPWLPA VTGYGTAMND AVSFIHATIS QLA

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Macromolecule #7: Light-harvesting protein LH1 Gamma-like

MacromoleculeName: Light-harvesting protein LH1 Gamma-like / type: protein_or_peptide / ID: 7 / Number of copies: 11 / Enantiomer: LEVO
Source (natural)Organism: Rhodopila globiformis (bacteria)
Molecular weightTheoretical: 2.542199 KDa
SequenceString:
(FME)AMVWMWILI APAIGIVLLS RQ

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Macromolecule #8: HEME C

MacromoleculeName: HEME C / type: ligand / ID: 8 / Number of copies: 4 / Formula: HEC
Molecular weightTheoretical: 618.503 Da
Chemical component information

ChemComp-HEC:
HEME C / Heme C

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Macromolecule #9: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-...

MacromoleculeName: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
type: ligand / ID: 9 / Number of copies: 18 / Formula: PGV
Molecular weightTheoretical: 749.007 Da
Chemical component information

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #10: BACTERIOCHLOROPHYLL A

MacromoleculeName: BACTERIOCHLOROPHYLL A / type: ligand / ID: 10 / Number of copies: 36 / Formula: BCL
Molecular weightTheoretical: 911.504 Da
Chemical component information

ChemComp-BCL:
BACTERIOCHLOROPHYLL A / Bacteriochlorophyll

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Macromolecule #11: BACTERIOPHEOPHYTIN A

MacromoleculeName: BACTERIOPHEOPHYTIN A / type: ligand / ID: 11 / Number of copies: 2 / Formula: BPH
Molecular weightTheoretical: 889.215 Da
Chemical component information

ChemComp-BPH:
BACTERIOPHEOPHYTIN A / Pheophytin

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Macromolecule #12: UBIQUINONE-10

MacromoleculeName: UBIQUINONE-10 / type: ligand / ID: 12 / Number of copies: 4 / Formula: U10
Molecular weightTheoretical: 863.343 Da
Chemical component information

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

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Macromolecule #13: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 13 / Number of copies: 5 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #14: FE (III) ION

MacromoleculeName: FE (III) ION / type: ligand / ID: 14 / Number of copies: 1 / Formula: FE
Molecular weightTheoretical: 55.845 Da

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Macromolecule #15: MENAQUINONE-9

MacromoleculeName: MENAQUINONE-9 / type: ligand / ID: 15 / Number of copies: 1 / Formula: MQ9
Molecular weightTheoretical: 785.233 Da
Chemical component information

ChemComp-MQ9:
MENAQUINONE-9 / Vitamin K2

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Macromolecule #16: (6~{E},8~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{E},24~...

MacromoleculeName: (6~{E},8~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{E},24~{E},26~{E},28~{E})-2,31-dimethoxy-2,6,10,14,19,23,27,31-octamethyl-dotriaconta-6,8,10,12,14,16,18,20,22,24,26,28-dodecaen-5-one
type: ligand / ID: 16 / Number of copies: 17 / Formula: I7D
Molecular weightTheoretical: 612.924 Da
Chemical component information

ChemComp-I7D:
(6~{E},8~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{E},24~{E},26~{E},28~{E})-2,31-dimethoxy-2,6,10,14,19,23,27,31-octamethyl-dotriaconta-6,8,10,12,14,16,18,20,22,24,26,28-dodecaen-5-one

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Macromolecule #17: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 17 / Number of copies: 9 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #18: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

MacromoleculeName: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / type: ligand / ID: 18 / Number of copies: 1 / Formula: PEE
Molecular weightTheoretical: 744.034 Da
Chemical component information

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM / Discrete optimized protein energy

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Macromolecule #19: water

MacromoleculeName: water / type: ligand / ID: 19 / Number of copies: 538 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5.0 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 277 K / Instrument: LEICA EM GP
DetailsThis sample was monodisperse.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average exposure time: 38.9 sec. / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationSoftware - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.24 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 128119
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5y5s

RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 40 / Target criteria: Correlation coefficient
Output model

PDB-7xxf:
Structure of photosynthetic LH1-RC super-complex of Rhodopila globiformis

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