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- EMDB-33285: Structure of human R-type voltage-gated CaV2.3-alpha2/delta1-beta... -

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Basic information

Entry
Database: EMDB / ID: EMD-33285
TitleStructure of human R-type voltage-gated CaV2.3-alpha2/delta1-beta1 channel complex in the ligand-free (apo) state
Map data
Sample
  • Complex: Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-beta1 complex
    • Protein or peptide: Voltage-dependent R-type calcium channel subunit alpha-1E
    • Protein or peptide: Voltage-dependent L-type calcium channel subunit beta-1
    • Protein or peptide: Voltage-dependent calcium channel subunit alpha-2/delta-1Voltage-gated calcium channel
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: HEXADECANE
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CALCIUM IONCalcium
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Function / homology
Function and homology information


positive regulation of muscle contraction / regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / Phase 2 - plateau phase / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / L-type voltage-gated calcium channel complex / high voltage-gated calcium channel activity / voltage-gated monoatomic cation channel activity ...positive regulation of muscle contraction / regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / Phase 2 - plateau phase / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / L-type voltage-gated calcium channel complex / high voltage-gated calcium channel activity / voltage-gated monoatomic cation channel activity / cardiac muscle cell action potential involved in contraction / NCAM1 interactions / regulation of ventricular cardiac muscle cell membrane repolarization / calcium ion transport into cytosol / regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / voltage-gated calcium channel complex / neuromuscular junction development / neuronal dense core vesicle / Phase 0 - rapid depolarisation / regulation of heart rate by cardiac conduction / regulation of calcium ion transport / calcium ion import across plasma membrane / voltage-gated calcium channel activity / sarcoplasmic reticulum / Regulation of insulin secretion / calcium ion transmembrane transport / cellular response to amyloid-beta / calcium ion transport / chemical synaptic transmission / neuronal cell body / synapse / calcium ion binding / extracellular exosome / metal ion binding / plasma membrane
Similarity search - Function
Voltage-dependent calcium channel, R-type, alpha-1 subunit / Voltage-dependent calcium channel, L-type, beta-1 subunit / Voltage-dependent L-type calcium channel subunit beta-1-4, N-terminal A domain / Voltage-dependent calcium channel, L-type, beta subunit / Voltage gated calcium channel subunit beta domain 4Aa N terminal / VWA N-terminal / Voltage-dependent calcium channel, alpha-2/delta subunit, conserved region / VWA N-terminal / Neuronal voltage-dependent calcium channel alpha 2acd / Voltage-dependent calcium channel, alpha-1 subunit, IQ domain ...Voltage-dependent calcium channel, R-type, alpha-1 subunit / Voltage-dependent calcium channel, L-type, beta-1 subunit / Voltage-dependent L-type calcium channel subunit beta-1-4, N-terminal A domain / Voltage-dependent calcium channel, L-type, beta subunit / Voltage gated calcium channel subunit beta domain 4Aa N terminal / VWA N-terminal / Voltage-dependent calcium channel, alpha-2/delta subunit, conserved region / VWA N-terminal / Neuronal voltage-dependent calcium channel alpha 2acd / Voltage-dependent calcium channel, alpha-1 subunit, IQ domain / Voltage gated calcium channel IQ domain / Voltage gated calcium channel IQ domain / Voltage-dependent calcium channel, alpha-1 subunit / Voltage-dependent L-type calcium channel, IQ-associated domain / Voltage-dependent L-type calcium channel, IQ-associated / Guanylate kinase/L-type calcium channel beta subunit / Guanylate kinase / Guanylate kinase homologues. / von Willebrand factor type A domain / von Willebrand factor (vWF) type A domain / VWFA domain profile. / Voltage-dependent channel domain superfamily / von Willebrand factor, type A / von Willebrand factor A-like domain superfamily / EF-hand calcium-binding domain profile. / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / EF-hand domain / Ion transport domain / Ion transport protein / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Voltage-dependent calcium channel subunit alpha-2/delta-1 / Voltage-dependent L-type calcium channel subunit beta-1 / Voltage-dependent R-type calcium channel subunit alpha-1E
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsGao Y / Qiu Y / Wei Y / Dong Y / Zhang XC / Zhao Y
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of SciencesXDB37030304 China
CitationJournal: Nat Commun / Year: 2023
Title: Molecular insights into the gating mechanisms of voltage-gated calcium channel Ca2.3.
Authors: Yiwei Gao / Shuai Xu / Xiaoli Cui / Hao Xu / Yunlong Qiu / Yiqing Wei / Yanli Dong / Boling Zhu / Chao Peng / Shiqi Liu / Xuejun Cai Zhang / Jianyuan Sun / Zhuo Huang / Yan Zhao /
Abstract: High-voltage-activated R-type Ca2.3 channel plays pivotal roles in many physiological activities and is implicated in epilepsy, convulsions, and other neurodevelopmental impairments. Here, we ...High-voltage-activated R-type Ca2.3 channel plays pivotal roles in many physiological activities and is implicated in epilepsy, convulsions, and other neurodevelopmental impairments. Here, we determine the high-resolution cryo-electron microscopy (cryo-EM) structure of human Ca2.3 in complex with the α2δ1 and β1 subunits. The VSD is stabilized in the resting state. Electrophysiological experiments elucidate that the VSD is not required for channel activation, whereas the other VSDs are essential for channel opening. The intracellular gate is blocked by the W-helix. A pre-W-helix adjacent to the W-helix can significantly regulate closed-state inactivation (CSI) by modulating the association and dissociation of the W-helix with the gate. Electrostatic interactions formed between the negatively charged domain on S6, which is exclusively conserved in the Ca2 family, and nearby regions at the alpha-interacting domain (AID) and S4-S5 helix are identified. Further functional analyses indicate that these interactions are critical for the open-state inactivation (OSI) of Ca2 channels.
History
DepositionApr 22, 2022-
Header (metadata) releaseFeb 8, 2023-
Map releaseFeb 8, 2023-
UpdateFeb 8, 2023-
Current statusFeb 8, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_33285.png

Downloads & links

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Map

FileDownload / File: emd_33285.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.45
Minimum - Maximum-3.500249 - 5.1120005
Average (Standard dev.)-0.002373848 (±0.10335529)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 332.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_33285_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_33285_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-b...

EntireName: Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-beta1 complex
Components
  • Complex: Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-beta1 complex
    • Protein or peptide: Voltage-dependent R-type calcium channel subunit alpha-1E
    • Protein or peptide: Voltage-dependent L-type calcium channel subunit beta-1
    • Protein or peptide: Voltage-dependent calcium channel subunit alpha-2/delta-1Voltage-gated calcium channel
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: HEXADECANE
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CALCIUM IONCalcium
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-b...

SupramoleculeName: Human R-type voltage-gated calcium channel CaV2.3-alpha2/delta1-beta1 complex
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1, #3, #2
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Voltage-dependent R-type calcium channel subunit alpha-1E

MacromoleculeName: Voltage-dependent R-type calcium channel subunit alpha-1E
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 262.055984 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MARFGEAVVA RPGSGDGDSD QSRNRQGTPV PASGQAAAYK QTKAQRARTM ALYNPIPVRQ NCFTVNRSLF IFGEDNIVRK YAKKLIDWP PFEYMILATI IANCIVLALE QHLPEDDKTP MSRRLEKTEP YFIGIFCFEA GIKIVALGFI FHKGSYLRNG W NVMDFIVV ...String:
MARFGEAVVA RPGSGDGDSD QSRNRQGTPV PASGQAAAYK QTKAQRARTM ALYNPIPVRQ NCFTVNRSLF IFGEDNIVRK YAKKLIDWP PFEYMILATI IANCIVLALE QHLPEDDKTP MSRRLEKTEP YFIGIFCFEA GIKIVALGFI FHKGSYLRNG W NVMDFIVV LSGILATAGT HFNTHVDLRT LRAVRVLRPL KLVSGIPSLQ IVLKSIMKAM VPLLQIGLLL FFAILMFAII GL EFYSGKL HRACFMNNSG ILEGFDPPHP CGVQGCPAGY ECKDWIGPND GITQFDNILF AVLTVFQCIT MEGWTTVLYN TND ALGATW NWLYFIPLII IGSFFVLNLV LGVLSGEFAK ERERVENRRA FMKLRRQQQI ERELNGYRAW IDKAEEVMLA EENK NAGTS ALEVLRRATI KRSRTEAMTR DSSDEHCVDI SSVGTPLARA SIKSAKVDGV SYFRHKERLL RISIRHMVKS QVFYW IVLS LVALNTACVA IVHHNQPQWL THLLYYAEFL FLGLFLLEMS LKMYGMGPRL YFHSSFNCFD FGVTVGSIFE VVWAIF RPG TSFGISVLRA LRLLRIFKIT KYWASLRNLV VSLMSSMKSI ISLLFLLFLF IVVFALLGMQ LFGGRFNFND GTPSANF DT FPAAIMTVFQ ILTGEDWNEV MYNGIRSQGG VSSGMWSAIY FIVLTLFGNY TLLNVFLAIA VDNLANAQEL TKDEQEEE E AFNQKHALQK AKEVSPMSAP NMPSIERDRR RRHHMSMWEP RSSHLRERRR RHHMSVWEQR TSQLRKHMQM SSQEALNRE EAPTMNPLNP LNPLSSLNPL NAHPSLYRRP RAIEGLALGL ALEKFEEERI SRGGSLKGDG GDRSSALDNQ RTPLSLGQRE PPWLARPCH GNCDPTQQEA GGGEAVVTFE DRARHRQSQR RSRHRRVRTE GKESSSASRS RSASQERSLD EAMPTEGEKD H ELRGNHGA KEPTIQEERA QDLRRTNSLM VSRGSGLAGG LDEADTPLVL PHPELEVGKH VVLTEQEPEG SSEQALLGNV QL DMGRVIS QSEPDLSCIT ANTDKATTES TSVTVAIPDV DPLVDSTVVH ISNKTDGEAS PLKEAEIRED EEEVEKKKQK KEK RETGKA MVPHSSMFIF STTNPIRRAC HYIVNLRYFE MCILLVIAAS SIALAAEDPV LTNSERNKVL RYFDYVFTGV FTFE MVIKM IDQGLILQDG SYFRDLWNIL DFVVVVGALV AFALANALGT NKGRDIKTIK SLRVLRVLRP LKTIKRLPKL KAVFD CVVT SLKNVFNILI VYKLFMFIFA VIAVQLFKGK FFYCTDSSKD TEKECIGNYV DHEKNKMEVK GREWKRHEFH YDNIIW ALL TLFTVSTGEG WPQVLQHSVD VTEEDRGPSR SNRMEMSIFY VVYFVVFPFF FVNIFVALII ITFQEQGDKM MEECSLE KN ERACIDFAIS AKPLTRYMPQ NRHTFQYRVW HFVVSPSFEY TIMAMIALNT VVLMMKYYSA PCTYELALKY LNIAFTMV F SLECVLKVIA FGFLNYFRDT WNIFDFITVI GSITEIILTD SKLVNTSGFN MSFLKLFRAA RLIKLLRQGY TIRILLWTF VQSFKALPYV CLLIAMLFFI YAIIGMQVFG NIKLDEESHI NRHNNFRSFF GSLMLLFRSA TGEAWQEIML SCLGEKGCEP DTTAPSGQN ENERCGTDLA YVYFVSFIFF CSFLMLNLFV AVIMDNFEYL TRDSSILGPH HLDEFVRVWA EYDRAACGRI H YTEMYEML TLMSPPLGLG KRCPSKVAYK RLVLMNMPVA EDMTVHFTST LMALIRTALD IKIAKGGADR QQLDSELQKE TL AIWPHLS QKMLDLLVPM PKASDLTVGK IYAAMMIMDY YKQSKVKKQR QQLEEQKNAP MFQRMEPSSL PQEIIANAKA LPY LQQDPV SGLSGRSGYP SMSPLSPQDI FQLACMDPAD DGQFQERQSL EPEVSELKSV QPSNHGIYLP SDTQEHAGSG RASS MPRLT VDPQVVTDPS SMRRSFSTIR DKRSNSSWLE EFSMERSSEN TYKSRRRSYH SSLRLSAHRL NSDSGHKSDT HRSGG RERG RSKERKHLLS PDVSRCNSEE RGTQADWESP ERRQSRSPSE GRSQTPNRQG TGSLSESSIP SVSDTSTPRR SRRQLP PVP PKPRPLLSYS SLIRHAGSIS PPADGSEEGS PLTSQALESN NACLTESSNS PHPQQSQHAS PQRYISEPYL ALHEDSH AS DCGEEETLTF EAAVATSLGR SNTIGSAPPL RHSWQMPNGH YRRRRRGGPG PGMMCGAVNN LLSDTEEDDK C

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Macromolecule #2: Voltage-dependent calcium channel subunit alpha-2/delta-1

MacromoleculeName: Voltage-dependent calcium channel subunit alpha-2/delta-1
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 122.034352 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: FPSAVTIKSW VDKMQEDLVT LAKTASGVNQ LVDIYEKYQD LYTVEPNNAR QLVEIAARDI EKLLSNRSKA LVRLALEAEK VQAAHQWRE DFASNEVVYY NAKDDLDPEK NDSEPGSQRI KPVFIEDANF GRQISYQHAA VHIPTDIYEG STIVLNELNW T SALDEVFK ...String:
FPSAVTIKSW VDKMQEDLVT LAKTASGVNQ LVDIYEKYQD LYTVEPNNAR QLVEIAARDI EKLLSNRSKA LVRLALEAEK VQAAHQWRE DFASNEVVYY NAKDDLDPEK NDSEPGSQRI KPVFIEDANF GRQISYQHAA VHIPTDIYEG STIVLNELNW T SALDEVFK KNREEDPSLL WQVFGSATGL ARYYPASPWV DNSRTPNKID LYDVRRRPWY IQGAASPKDM LILVDVSGSV SG LTLKLIR TSVSEMLETL SDDDFVNVAS FNSNAQDVSC FQHLVQANVR NKKVLKDAVN NITAKGITDY KKGFSFAFEQ LLN YNVSRA NCNKIIMLFT DGGEERAQEI FNKYNKDKKV RVFTFSVGQH NYDRGPIQWM ACENKGYYYE IPSIGAIRIN TQEY LDVLG RPMVLAGDKA KQVQWTNVYL DALELGLVIT GTLPVFNITG QFENKTNLKN QLILGVMGVD VSLEDIKRLT PRFTL CPNG YYFAIDPNGY VLLHPNLQPK PIGVGIPTIN LRKRRPNIQN PKSQEPVTLD FLDAELENDI KVEIRNKMID GESGEK TFR TLVKSQDERY IDKGNRTYTW TPVNGTDYSL ALVLPTYSFY YIKAKLEETI TQARYSETLK PDNFEESGYT FIAPRDY CN DLKISDNNTE FLLNFNEFID RKTPNNPSCN ADLINRVLLD AGFTNELVQN YWSKQKNIKG VKARFVVTDG GITRVYPK E AGENWQENPE TYEDSFYKRS LDNDNYVFTA PYFNKSGPGA YESGIMVSKA VEIYIQGKLL KPAVVGIKID VNSWIENFT KTSIRDPCAG PVCDCKRNSD VMDCVILDDG GFLLMANHDD YTNQIGRFFG EIDPSLMRHL VNISVYAFNK SYDYQSVCEP GAAPKQGAG HRSAYVPSVA DILQIGWWAT AAAWSILQQF LLSLTFPRLL EAVEMEDDDF TASLSKQSCI TEQTQYFFDN D SKSFSGVL DCGNCSRIFH GEKLMNTNLI FIMVESKGTC PCDTRLLIQA EQTSDGPNPC DMVKQPRYRK GPDVCFDNNV LE DYTDCGG VSGLNPSLWY IIGIQFLLLW LVSGSTHRLL

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Macromolecule #3: Voltage-dependent L-type calcium channel subunit beta-1

MacromoleculeName: Voltage-dependent L-type calcium channel subunit beta-1
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.799594 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MVQKTSMSRG PYPPSQEIPM EVFDPSPQGK YSKRKGRFKR SDGSTSSDTT SNSFVRQGSA ESYTSRPSDS DVSLEEDREA LRKEAERQA LAQLEKAKTK PVAFAVRTNV GYNPSPGDEV PVQGVAITFE PKDFLHIKEK YNNDWWIGRL VKEGCEVGFI P SPVKLDSL ...String:
MVQKTSMSRG PYPPSQEIPM EVFDPSPQGK YSKRKGRFKR SDGSTSSDTT SNSFVRQGSA ESYTSRPSDS DVSLEEDREA LRKEAERQA LAQLEKAKTK PVAFAVRTNV GYNPSPGDEV PVQGVAITFE PKDFLHIKEK YNNDWWIGRL VKEGCEVGFI P SPVKLDSL RLLQEQKLRQ NRLGSSKSGD NSSSSLGDVV TGTRRPTPPA SAKQKQKSTE HVPPYDVVPS MRPIILVGPS LK GYEVTDM MQKALFDFLK HRFDGRISIT RVTADISLAK RSVLNNPSKH IIIERSNTRS SLAEVQSEIE RIFELARTLQ LVA LDADTI NHPAQLSKTS LAPIIVYIKI TSPKVLQRLI KSRGKSQSKH LNVQIAASEK LAQCPPEMFD IILDENQLED ACEH LAEYL EAYWKATHPP SSTPPNPLLN RTMATAALAA SPAPVSNLQG PYLASGDQPL ERATGEHASM HEYPGELGQP PGLYP SSHP PGRAGTLRAL SRQDTFDADT PGSRNSAYTE LGDSCVDMET DPSEGPGLGD PAGGGTPPAR QGSWEDEEED YEEELT DNR NRGRNKARYC AEGGGPVLGR NKNELEGWGR GVYIR

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Macromolecule #6: CHOLESTEROL HEMISUCCINATE

MacromoleculeName: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 6 / Number of copies: 6 / Formula: Y01
Molecular weightTheoretical: 486.726 Da
Chemical component information

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

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Macromolecule #7: HEXADECANE

MacromoleculeName: HEXADECANE / type: ligand / ID: 7 / Number of copies: 7 / Formula: R16
Molecular weightTheoretical: 226.441 Da
Chemical component information

ChemComp-R16:
HEXADECANE / Hexadecane

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Macromolecule #8: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 8 / Number of copies: 1 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

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Macromolecule #9: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 9 / Number of copies: 2 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #10: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 10 / Number of copies: 8 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
DetailsThis sample was obtained from the monodispersed peak fractions of the size-exclusion chromatography.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 7676 pixel / Digitization - Dimensions - Height: 7420 pixel / Digitization - Frames/image: 1-32 / Number grids imaged: 1 / Number real images: 2097 / Average exposure time: 6.7 sec. / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 787518
Startup modelType of model: INSILICO MODEL
In silico model: The insilico model was generated using the ab-initio reconstruction in cryoSPARC.
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final 3D classificationNumber classes: 6 / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.1) / Number images used: 257473

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Atomic model buiding 1

Initial modelPDB ID:

7vfs

RefinementProtocol: RIGID BODY FIT
Output model

PDB-7xlq:
Structure of human R-type voltage-gated CaV2.3-alpha2/delta1-beta1 channel complex in the ligand-free (apo) state

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