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- EMDB-31353: Barley PSI-LHCI-NDH supercomplex -

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Basic information

Entry
Database: EMDB / ID: EMD-31353
TitleBarley PSI-LHCI-NDH supercomplex
Map data
Sample
  • Complex: PSI-LHCI-Lhca6 supercomplex of Barley
Biological speciesHordeum vulgare subsp. spontaneum (wild barley)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.45 Å
AuthorsWang WD / Shen L / Tang KL / Han GY / Shen JR / Zhang X
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of SciencesQYZDY-SSW-SMC003 China
CitationJournal: To be published
Title: Barley PSI-LHCI-NDH supercomplex
Authors: Wang WD / Shen L / Tang KL / Han GY / Shen JR / Zhang X
History
DepositionMay 25, 2021-
Header (metadata) releaseJun 8, 2022-
Map releaseJun 8, 2022-
UpdateJun 8, 2022-
Current statusJun 8, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_31353.png

Downloads & links

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Map

FileDownload / File: emd_31353.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.307 Å
Density
Contour LevelBy AUTHOR: 0.457
Minimum - Maximum-0.66772294 - 1.8704193
Average (Standard dev.)-0.0007180982 (±0.061866987)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 575.08 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : PSI-LHCI-Lhca6 supercomplex of Barley

EntireName: PSI-LHCI-Lhca6 supercomplex of Barley
Components
  • Complex: PSI-LHCI-Lhca6 supercomplex of Barley

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Supramolecule #1: PSI-LHCI-Lhca6 supercomplex of Barley

SupramoleculeName: PSI-LHCI-Lhca6 supercomplex of Barley / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#15
Source (natural)Organism: Hordeum vulgare subsp. spontaneum (wild barley)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.45 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 551110

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