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- EMDB-29552: Structure of NOT1:NOT10:NOT11 module of the chicken CCR4-NOT complex -
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Open data
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Basic information
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Title | Structure of NOT1:NOT10:NOT11 module of the chicken CCR4-NOT complex | |||||||||
![]() | DeepEMhancer map | |||||||||
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![]() | mRNA degradation / ![]() ![]() | |||||||||
Function / homology | ![]() Deadenylation of mRNA / TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain / ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Lea SM / Deme JC / Raisch T / Levdansky Y / Valkov E | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and assembly of the NOT10:11 module of the CCR4-NOT complex. Authors: Yevgen Levdansky / Tobias Raisch / Justin C Deme / Filip Pekovic / Hans Elmlund / Susan M Lea / Eugene Valkov / ![]() ![]() Abstract: NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT ...NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 182.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 21.9 KB 21.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.7 KB | Display | ![]() |
Images | ![]() | 34 KB | ||
Masks | ![]() | 216 MB | ![]() | |
Others | ![]() ![]() ![]() ![]() | 107.4 MB 203.7 MB 200.8 MB 200.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8fy4MC ![]() 8fy3C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | DeepEMhancer map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.723 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Additional map: Unsharpened map
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-Additional map: Sharpened map
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Annotation | Sharpened map | ||||||||||||
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-Half map: half map A
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Annotation | half map A | ||||||||||||
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-Half map: half map B
File | emd_29552_half_map_2.map | ||||||||||||
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Annotation | half map B | ||||||||||||
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Density Histograms |
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Sample components
-Entire : NOT1:NOT10:NOT11
Entire | Name: NOT1:NOT10:NOT11 |
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Components |
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-Supramolecule #1: NOT1:NOT10:NOT11
Supramolecule | Name: NOT1:NOT10:NOT11 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: CCR4-NOT transcription complex subunit 1
Macromolecule | Name: CCR4-NOT transcription complex subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 76.422859 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD GKSSGKDFHQ TQFLIQECAS LITKPNFIS TLSYAIDNPL HYQKSLKPSP HLFAQLSKVI KLSKVQEVIF GLALLNSFSS DLRGFAAQFI KQKLPDLLRS Y IDADVSGN ...String: MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD GKSSGKDFHQ TQFLIQECAS LITKPNFIS TLSYAIDNPL HYQKSLKPSP HLFAQLSKVI KLSKVQEVIF GLALLNSFSS DLRGFAAQFI KQKLPDLLRS Y IDADVSGN QEGGFQDIAI EVLHLLLSHL LFGQKGAFGV GQEQIDAFLK TLRRDFPQER CPVVLAPLLY PEKRDILMDR IL PDSGGIA KTMMESSLAD FMQEVGYGFC TSIEECRNII MQFGVREVTA AQVARVLGMM ARTHSGLTDG IPLQSISAPG SGI WSDGKD KSDGAQAHTW NVEVLIDVLK ELNPSLDFKV VTYELDHPGF QIRDSKGLHI VVFGIQRGLG MEVFPVNAIY RPWK HAEGQ LSFIQHSLIN PDIFCFADYP CHAVTTDILK APPEDDNREI ATWKSLDLIE SLLRLAEVGQ YEQVKQLFSF PIKHC PDML VLALLQINTS WHTLRHELIS TLMPIFLGNH PNSAIILHYA WHGQGQSPSI RQLIMHAMAE WYMRGEQYDQ AKLSRI LDV AQDLKALSML LNGTPFAFVI DLAALASRRE YLKLDKWLTD KIREHGEPFI QACMTFLKRR CPSILGGLAP EKDQPKS AQ LPPETLATML ACLQACAGSV SQELSETILT MVANCSNVMN UniProtKB: ![]() |
-Macromolecule #2: CCR4-NOT transcription complex subunit 10
Macromolecule | Name: CCR4-NOT transcription complex subunit 10 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 75.955148 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MDQEKELSSS ALQAFLAGNY DACLQHLNTL QDINKDDYKI TLNTAVAEFC KSNQTTTDNL RQTLNQLKNQ VHSAVEEMDG LDDVENSML YYNQAVILYH LRQYTEAISV GEKLYQFIEP FEEKFAQAVC FLLVDLYLLT YQAEKALHLL AVLEKMISQG N NNSKNGKN ...String: MDQEKELSSS ALQAFLAGNY DACLQHLNTL QDINKDDYKI TLNTAVAEFC KSNQTTTDNL RQTLNQLKNQ VHSAVEEMDG LDDVENSML YYNQAVILYH LRQYTEAISV GEKLYQFIEP FEEKFAQAVC FLLVDLYLLT YQAEKALHLL AVLEKMISQG N NNSKNGKN ESGNNTNKDS SNQKAESGAL IEVAKSKIHQ YKVRAYIQMK SLKACKREIK SVMNTAGNSA PSLFLKSNFE YL RGNYRKA VKLLNSANIA EHPGFMKTGE CLRCMFWNNL GCIHFAMGKH NLGIFYFKKA LQENDNACAQ LGTGSSDPGK KFS GRPMCT LLTNKRYELL YNCGIQLLHI GRPLAAFECL IEAVQVYHSN PRLWLRIAEC CIAANKGTSE QETKGLPSKK GIVQ SIVGQ GYHRKIVLAS QSIQNVVYND GQSSAIPVAS MEFAAICLRN ALLLLPEDQQ EPKQENGSKP NNQLGGNTEN SESSE ACSN KSHEGDKFIA APPSSPLKKQ ELENLRCSIL ACSAYVALAL GDNLMALNHA DKLLQQPKLS GSLKFLGHLY AAEALI SLD RISDAITHLN PENVTDVSLT VSSNEQDQGS DKGENEAMES SGKQTPQCYP SSVTSARTMM LFNLGSAYCL RSEYDKA RK CLHQAASLIH PKEIPPEAIL LAVYLELQNG NTQLALQIIK RNQ UniProtKB: CCR4-NOT transcription complex subunit 10 |
-Macromolecule #3: CCR4-NOT transcription complex subunit 11
Macromolecule | Name: CCR4-NOT transcription complex subunit 11 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 51.950637 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MLERMSLSPK ELTSLLGIVS EEAGGSTFEG LSSAFHHYFG KAEHFRLGSV LVLLLQQPDL LPSPAQRLTA LYLLWEMYRT EPLAANPFA AVFAHLLNPA PERGGDEAER TPLSGFLPPI TPPEKFFLSQ LMLAPPRELF KKTPRQIAAM DVGNMAQSLG I SGLQLALA ...String: MLERMSLSPK ELTSLLGIVS EEAGGSTFEG LSSAFHHYFG KAEHFRLGSV LVLLLQQPDL LPSPAQRLTA LYLLWEMYRT EPLAANPFA AVFAHLLNPA PERGGDEAER TPLSGFLPPI TPPEKFFLSQ LMLAPPRELF KKTPRQIAAM DVGNMAQSLG I SGLQLALA ERQSELPTQS KASFPSILSD PDPDSSNSGF DSSVASQITE ALVSGPKPPI ESHFRPEFIR PPPPLHICED EL AWLNPIE PDHTIQWDKS MCVKNSTGVE IKRIMAKAFK SPLTSPQQTQ LLGELEKDPK LVYHIGLTPA KLPDLVENNP LVA IEMLLK LMQSSQITEY FSVLVNMDMS LHSMEVVNRL TTAVDLPPEF IHLYISNCIS TCEQIKDKYM QNRLVRLVCV FLQS LIRNK IINVQDLFIE VQAFCIEFSR IREAAGLFRL LKTLDTGSEN LYFQGSGAMG SGSGHHHHHH GT UniProtKB: CCR4-NOT transcription complex subunit 11 |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 55.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |