+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-25151 | |||||||||
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Title | Antibody A7V3 bound to SARS-CoV-2 spike | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Severe acute respiratory syndrome coronavirus 2 / Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.99 Å | |||||||||
Authors | Hsieh C-L / McLellan JS | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Discovery of potent SARS-CoV-2 neutralizing antibodies and a novel quaternary binding mode Authors: Goike J / Hsieh C-L / McLellan JS / Gollihar JD | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_25151.map.gz | 257 MB | EMDB map data format | |
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Header (meta data) | emd-25151-v30.xml emd-25151.xml | 12.8 KB 12.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_25151_fsc.xml | 15 KB | Display | FSC data file |
Images | emd_25151.png | 80.1 KB | ||
Others | emd_25151_half_map_1.map.gz emd_25151_half_map_2.map.gz | 285.7 MB 285.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-25151 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-25151 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_25151.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_25151_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_25151_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of A7V3 Fab bound to SARS-CoV-2 spike
Entire | Name: Complex of A7V3 Fab bound to SARS-CoV-2 spike |
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Components |
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-Supramolecule #1: Complex of A7V3 Fab bound to SARS-CoV-2 spike
Supramolecule | Name: Complex of A7V3 Fab bound to SARS-CoV-2 spike / type: complex / ID: 1 / Parent: 0 |
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-Supramolecule #2: SARS-CoV-2 spike
Supramolecule | Name: SARS-CoV-2 spike / type: complex / ID: 2 / Parent: 1 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Recombinant expression | Organism: Homo sapiens (human) |
-Supramolecule #3: A7V3 Fab
Supramolecule | Name: A7V3 Fab / type: complex / ID: 3 / Parent: 1 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 / Details: 2 mM Tris pH 7.5, 200 mM NaCl, 0.02% NaN3 |
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Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Pretreatment - Type: PLASMA CLEANING |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV |
Details | Collected on UltrAuFoil 1.2-1.3 with 0.2 mg/mL HexaPro-3 and 2x fold molar excess FabA7V3 spiked in ~30 minutes before freezing. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 80.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |