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- EMDB-23083: Outer dynein arm core subcomplex from C. reinhardtii -

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Basic information

Entry
Database: EMDB / ID: EMD-23083
TitleOuter dynein arm core subcomplex from C. reinhardtii
Map datacomposite ODA core map from focused refinements
Sample
  • Complex: ODA core subcomplex
    • Protein or peptide: x 16 types
Keywordsdynein / microtubule / cilia / MOTOR PROTEIN
Function / homology
Function and homology information


outer dynein arm / outer dynein arm assembly / cilium movement involved in cell motility / 9+2 motile cilium / dynein light chain binding / cilium movement / motile cilium assembly / dynein heavy chain binding / cell projection organization / dynein complex ...outer dynein arm / outer dynein arm assembly / cilium movement involved in cell motility / 9+2 motile cilium / dynein light chain binding / cilium movement / motile cilium assembly / dynein heavy chain binding / cell projection organization / dynein complex / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / ciliary plasm / motile cilium / dynein intermediate chain binding / microtubule-based movement / axoneme / microtubule-based process / enzyme regulator activity / microtubule / calcium ion binding / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Kelch repeat type 2 / Kelch motif / Galactose oxidase, central domain / Dynein light chain roadblock-type 1/2 / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Phospholipase D/Transphosphatidylase / Phospholipase D phosphodiesterase active site profile. / Dynein light chain, type 1/2, conserved site ...Kelch repeat type 2 / Kelch motif / Galactose oxidase, central domain / Dynein light chain roadblock-type 1/2 / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Phospholipase D/Transphosphatidylase / Phospholipase D phosphodiesterase active site profile. / Dynein light chain, type 1/2, conserved site / Dynein light chain type 1 signature. / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / Filamin/ABP280 repeat / Filamin-type immunoglobulin domains / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Galactose oxidase/kelch, beta-propeller / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / IPT/TIG domain / Kelch-type beta propeller / IPT domain / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Immunoglobulin E-set / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
PLD phosphodiesterase domain-containing protein / Dynein light chain roadblock / Uncharacterized protein / Dynein light chain / Mr19,000 outer arm dynein light chain / Dynein, 70 kDa intermediate chain, flagellar outer arm / Dynein light chain 9 / Dynein gamma chain, flagellar outer arm / Dynein, 78 kDa intermediate chain, flagellar outer arm / Dynein 11 kDa light chain, flagellar outer arm ...PLD phosphodiesterase domain-containing protein / Dynein light chain roadblock / Uncharacterized protein / Dynein light chain / Mr19,000 outer arm dynein light chain / Dynein, 70 kDa intermediate chain, flagellar outer arm / Dynein light chain 9 / Dynein gamma chain, flagellar outer arm / Dynein, 78 kDa intermediate chain, flagellar outer arm / Dynein 11 kDa light chain, flagellar outer arm / Dynein 8 kDa light chain, flagellar outer arm / Dynein 18 kDa light chain, flagellar outer arm / Outer dynein arm-docking complex protein DC3 / Dynein light chain roadblock
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodhelical reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsWalton T / Wu H
CitationJournal: Nat Commun / Year: 2021
Title: Structure of a microtubule-bound axonemal dynein.
Authors: Travis Walton / Hao Wu / Alan Brown /
Abstract: Axonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating, a process critical for cellular motility and extracellular fluid flow. Axonemal dyneins are evolutionarily ...Axonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating, a process critical for cellular motility and extracellular fluid flow. Axonemal dyneins are evolutionarily and biochemically distinct from cytoplasmic dyneins that transport cargo, and the mechanisms regulating their localization and function are poorly understood. Here, we report a single-particle cryo-EM reconstruction of a three-headed axonemal dynein natively bound to doublet microtubules isolated from cilia. The slanted conformation of the axonemal dynein causes interaction of its motor domains with the neighboring dynein complex. Our structure shows how a heterotrimeric docking complex specifically localizes the linear array of axonemal dyneins to the doublet microtubule by directly interacting with the heavy chains. Our structural analysis establishes the arrangement of conserved heavy, intermediate and light chain subunits, and provides a framework to understand the roles of individual subunits and the interactions between dyneins during ciliary waveform generation.
History
DepositionDec 10, 2020-
Header (metadata) releaseJan 20, 2021-
Map releaseJan 20, 2021-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7kzn
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7kzn
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_23083.png

Downloads & links

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Map

FileDownload / File: emd_23083.map.gz / Format: CCP4 / Size: 67 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcomposite ODA core map from focused refinements
Voxel sizeX=Y=Z: 1.36 Å
Density
Contour LevelBy AUTHOR: 0.0125 / Movie #1: 0.0125
Minimum - Maximum-0.0044215075 - 0.14988564
Average (Standard dev.)0.0009152786 (±0.0025986836)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions260260260
Spacing260260260
CellA=B=C: 353.6 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.361.361.36
M x/y/z260260260
origin x/y/z0.0000.0000.000
length x/y/z353.600353.600353.600
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS260260260
D min/max/mean-0.0040.1500.001

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Supplemental data

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Mask #1

Fileemd_23083_msk_1.map
Projections & Slices
AxesZYX

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Additional map: focused refinement around IC2

Fileemd_23083_additional_1.map
Annotationfocused refinement around IC2
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Additional map: half map 1 for focused refinement around IC1 and LC7a/b

Fileemd_23083_additional_10.map
Annotationhalf map 1 for focused refinement around IC1 and LC7a/b
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Additional map: half map 2 for focused refinement around IC1 and LC7a/b

Fileemd_23083_additional_11.map
Annotationhalf map 2 for focused refinement around IC1 and LC7a/b
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Additional map: half map 2 for focused refinement around IC2

Fileemd_23083_additional_12.map
Annotationhalf map 2 for focused refinement around IC2
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Additional map: refinement of the entire ODA core

Fileemd_23083_additional_13.map
Annotationrefinement of the entire ODA core
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Additional map: half map 2 for refinement of the entire ODA core

Fileemd_23083_additional_14.map
Annotationhalf map 2 for refinement of the entire ODA core
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Additional map: half map 1 for refinement of the entire ODA core

Fileemd_23083_additional_15.map
Annotationhalf map 1 for refinement of the entire ODA core
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Additional map: mask for refinement of the entire ODA core

Fileemd_23083_additional_16.map
Annotationmask for refinement of the entire ODA core
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Additional map: mask for focused refinement around IC1 and LC7a/b

Fileemd_23083_additional_2.map
Annotationmask for focused refinement around IC1 and LC7a/b
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Additional map: mask for focused refinement around LC8

Fileemd_23083_additional_3.map
Annotationmask for focused refinement around LC8
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Additional map: half map 1 for focused refinement around IC2

Fileemd_23083_additional_4.map
Annotationhalf map 1 for focused refinement around IC2
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Additional map: half map 1 for focused refinement around LC8

Fileemd_23083_additional_5.map
Annotationhalf map 1 for focused refinement around LC8
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Additional map: mask for focused refinement around IC2

Fileemd_23083_additional_6.map
Annotationmask for focused refinement around IC2
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Additional map: half map 2 for focused refinement around LC8

Fileemd_23083_additional_7.map
Annotationhalf map 2 for focused refinement around LC8
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Additional map: focused refinement around LC8

Fileemd_23083_additional_8.map
Annotationfocused refinement around LC8
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Additional map: focused refinement around IC1 and LC7a/b

Fileemd_23083_additional_9.map
Annotationfocused refinement around IC1 and LC7a/b
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Sample components

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Entire : ODA core subcomplex

EntireName: ODA core subcomplex
Components
  • Complex: ODA core subcomplex
    • Protein or peptide: Heavy chain alpha
    • Protein or peptide: Flagellar outer dynein arm heavy chain beta
    • Protein or peptide: Dynein gamma chain, flagellar outer arm
    • Protein or peptide: Dynein, 78 kDa intermediate chain, flagellar outer arm
    • Protein or peptide: Dynein, 70 kDa intermediate chain, flagellar outer arm
    • Protein or peptide: Flagellar outer dynein arm light chain 2
    • Protein or peptide: Dynein 18 kDa light chain, flagellar outer arm
    • Protein or peptide: Dynein 11 kDa light chain, flagellar outer arm
    • Protein or peptide: Dynein light chain roadblock LC7a
    • Protein or peptide: Dynein light chain roadblock LC7b
    • Protein or peptide: Dynein 8 kDa light chain, flagellar outer arm
    • Protein or peptide: Dynein light chain 9
    • Protein or peptide: Dynein light chain 10
    • Protein or peptide: DC1
    • Protein or peptide: DC2
    • Protein or peptide: Outer dynein arm-docking complex protein DC3

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Supramolecule #1: ODA core subcomplex

SupramoleculeName: ODA core subcomplex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Details: IC-LC block with partial N-terminal tails of the heavy chains
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: Heavy chain alpha

MacromoleculeName: Heavy chain alpha / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 504.949312 KDa
SequenceString: MSIFWEVPNA QGEAPCPRSG HSFTVLGERF VLFGGCGRKD GKAAAFNDLY ELDTSDPDEY KWKELVVANA PPPRARHAAI ALDDKRLLV FGGLNKRIRY NDVWLFNYDD KSWTCMEVEG AAPEPRAHFT ATRFGSRVFI FGGYGGSGQV YNEMWVLHFG E DGFRWQNI ...String:
MSIFWEVPNA QGEAPCPRSG HSFTVLGERF VLFGGCGRKD GKAAAFNDLY ELDTSDPDEY KWKELVVANA PPPRARHAAI ALDDKRLLV FGGLNKRIRY NDVWLFNYDD KSWTCMEVEG AAPEPRAHFT ATRFGSRVFI FGGYGGSGQV YNEMWVLHFG E DGFRWQNI TESIEGTGPA PRFDHSAFIY PVTPNSDTYD KLLIMGGRDL SQMYQDSHML DLNKMAWENE TQPPTLPYEI CN NVCDGIE SVPYHKVFSF GGRKGMMQYL NTVEVMDCGS QMWSTPPVDH GVAPVGREDT AWVFDVKTCS LLIFGGWANR WLG DLHKLN VSPIIGPPYA CTAIQPEMGP VFGSTELVIR GLRFRDGKVQ VKFGLSEKNE VVVEGTYVDQ ETIRVQTPNY EQFG ALTVD VRVSINGEGW TVNKIKYAYF ANTAARNCIA YGPGLLAETI SGVEVPFIIQ AKDTLNDKRT SGGDVFKVTV VSADG KNEG VSRVRDLQNG QYEVQYAAPT AGPYLIHVAF NELGTSDFVP IRGSPFTVKC TDSWTKHRVM GAAPAKRKGA TICTMG NEL VLYGGDKSGV TVLNTEGAEW RWSPATVSGS TPPDRTAHST VVLSDGELVV FGGINLADQN DLNDIYYLRK QGEGWVW SC PSESRPYIRH PKGAAAVSAE PSAEPAAEPA AEPAAEPDAD APAAEPAAEG EEGAVPAEGE EGAEGASGSR PVSAKPAP A AAAPAAEALP ELPVSARNSH VAVAIDKDLY VMMGDHDGDL MTELAMVDTS DRTCAHWLEP ILKGDVPVPR KACAAAATG NTIVLFGGQT QNADGEATVT GDLVIMEVTG PNSIQCAVNP AAPGASGSPA ARYGAVMQEF SNGKLFLHGG MDAASKPLND GWLFDVPSK TWQCVYVGSS DVVLPTGSLA TLWRNRIVLV SAAVGSPKLD SVQSLDFQEL RDSVAFTPKM RASTETLLKG L EDWVDTQA HGMELARSPE KLSKDFENGL RKVMDALFQV KSQRSQTDLL IDQLHEAFAQ LAEEKVPGIN KMEKRLEAAA HK WDEIKKA QPQVKTDVEP IQAAKGEDIK KEIETFAAKV RNYRADFRRR GFFKYATGFD GAYPLLDAAA HELAELKKEC DRL SELASV FEFPQAIEPV TVAIKETVED LVMVKDVWDT AVLCELQFQD WRQTLWSDIR TDIMEEGAKQ FVKEVKSLHK KVRD EDVFR GVDQVVKNFL VSVPLVADLR SPAMRDRHWE QLMATTKMTF NVKDPNFKLD DLLALELHKF EEEVGEIVDR AQKEE KMEI AIRKLNDTWT RVEFQFHRHK DYDVHTVKMA EEDFEALEDN QVQVQGMIAN RYMATFKDEI LGWQKKLNDV ADVNQI MAE IQRTWAYLES LFIHSEEVKK ELPQATERFA AIDTEVKKVL REFQQLKNCV SCCNREGLYA NLETQERELE ICKKALN DY MESKRRAFPR FYFVSSADLL DILSNGNNPM RVQIHMNKCF QAIDKLRLDS EEVVPGRRPK ALGMESCVGI EYVPFSSP L PLENKVEQYM NDIIAKMRNE LRMVLKASVE DYPSKPRDKW LFDWPSQIIL VVNQIYWCLE VEQAFTEMAR GDKGAMSKY NEFQVKQLTK LIEVTRTDLS KPDRQKIMNM ITIDAHSRDM VLAVIEAGAD QPDSFQWVSQ LRSYWDRDIS DCRIRICDAS FPYGYEYLG NGPRLVITPL TDRIYITATQ ACWLSLGTAP AGPAGTGKTE TTKDLSAQLG KSVYVFNCSP EMDYRTMGDI F KGLAASGS WGCFDEFNRL VPEVLSVCSV QYKCVTDSQK KKTMLPGRGL EYIKDGVKHP AVEHWSFIAA DGVEMPLEEG TS AFITMNP GYIGRAELPE SLKALFRPIT VMVPDRQLIM ENMLMAEGFV EAKMLAKKFA SLYYLLEDLL SPQKHYDWGL RAI KSVLVV AGSLLRAEAG QVEADVLFRA LRDFNIPKIL AQDMVIFMGL LNDLFPGIDP PRKRDMEFED VIVSTIKDLG LTPE DDFVL RVVQFSELLA IRHCVFLMGP TGTGRTECYR VLAKAITKGC NNPVNDYLKM TNKKKVVIRD INPKSISTYE LYGQV NQAT REWKDGLLSY YMRELANMPG DDPKWLLLDG DLDANWIESM NSVMDDNRLL TLPSNERIRV LPHMKLIFEI RDLKFA TPA TATRAGILYI SEGQQWHNMA MSWINRVVKP YAERAKWKDP QLPCTWLREM FDKYIPPTLL EMKKSYSHIT PLAQMNF IS TLVNIMEGVL KPENLSNKAD QAMFEMYFVF AMIWAFGGGL VEKDGIPYRR NFDKWFKQTW TTVKIPGKGT VYDYFVNP K TQKFQPWAEL VTDIDYDGSR PMSTVFVPTA ETSSLRFFLD MMVDLRKPIM FVGGAGVGKT QLVKGKLGSL NEEQISLSI SFNYFTDVVS FQKVLESPLE KKAGINYGPP GTKQLIYFVD DLNMPKLDLY ETAMPISLIR QHLGWGHWFD RAKLTPKNIN NTQYVACMN PTAGSFIINP RLQRLFMTLA VDFPGQDSLM KIYGTFLQGH LKKFSESIQD MGTKILQAAL ALHDRVSQTF R KTAINFHY EFTVRHLANV FQGLLMSTPE AFNSPTKWGK LWLHESERVY ADRLVSLYDL DAYNKAATAI AKKYFSVADI DD YYKKKDP KPLIFCHFAR GLADKAYDEV ADYTSLYKTL TEALNEYNET NAAMDLVLFE DAMKHVCRIS RIVSNPSGHA LLV GVGGSG KQSLARLAAH ICGYATQMIV ISGSYSMNNF KEDIQKMYKR TGVKGEGVMF LFTDSQIVDE RMLVYINDLL SSGE IPDLF PQEDRDEIVN ALRSETKSLG LLDTAENCWA TFIQKVKTNL HMVFTASPVG ENFRVRSQRF LATVTSTVID WFQPW PESS LFSVAKRFLD EVDLGEDAVR NAVVEFMPYS FQLVNKVSIK FREQERRYNY TTPKTFLELI KLYKNVLAAK RKANQD NTE RLENGLHKLH KVQADVDILV EEAKVKAVEV EHKVASANIF AEQVGVEKEK VNAENAAAQV EAEKCAVIAK EVSEKQA SC EKDLAAAEPL VAEAMAALET VTKKDLGEAK SLKKPPPGVD DITAVVIILL ENNPKDKSWQ AAQKLMNNVD KFLERVKS F KSVIDAGQVA RKTVDACRPY LALEWFNREA IGKKSAAAAG LCEWAVNIIK YYDVVQEVEP KRQELAAANA KLEEANVTL AAVEEKVALL NAKVQELEQQ YKEANDDKEA AIRESERCQR KLELANRLIN ALASEGERWA LTVEQLRKSY EVLTGDMLLA AAFVSYAGP FTAKFRAQLI DDWILFLRER HMPMTEGITD PLKVLVDDAL VAGWIREGLP SDPTSVQNGT ILTNSERWSL M MDPQLQGI LWIKERESKN NLQVTRMGAS NMLQVMERAI EAGHSVLVEN MGETIDAVLN PIITRSTFKK GRSLYVKLGD KE CEYNKNF RLFLHTKLSN PHYPPEIQAE TTLINFTVTE AGLEDQLLAL VVNKERPDLE ETKTQLIIQN TEFTIKLKEL EDG LLLKLS TAEGDITEDV ALIESLEDAK RVSTEISEKV KESRETEAAI NENRNKYRTV AARGAMLFFL LNSLNKIHAF YQFS LNAFV TVFSRGLDLA PGGRKKGKGL KKTPSLRDQP MDHQSLMEKA RRSSGVGDRR PSQEGLPGPE ASQASLAESQ GGRGS QVGD AEDEDDESFA MAPEALEQRL VNLLETCTFT VYNYTRRGLF DRDKLIVLSL LTFTILLRSQ AVDASEYEAL CRGMRN PTP PPITDDLSRW MAESQWAALD VLTTLPCFAH LAKDMEKNSD DWFNWCNNEA AERAPMPGEW GKLTEFRQLL IIRALRP DR ITNALQNFCE HMMGSDYVNQ DAFSPAAMMD ESSSATPIFF ILFPGYSPSK EIEVYANKCG YSVANGRLCL ISMGQGQE A PAEAVLDKYT REGGWVFLDN VHLMQGWIPK LERKLEIAAE SAHPDFRCFF SAEPINGAPH ANIIPESILQ TCIKISNEP PSDMKSNMRR AFAAFTPEQC DRPSTPAKRV AFRAILFGLC FYHSLLLGRK KFGVGIGTGS GSGLGFCRGY SFNIGDLTTC GDVLYNYLE AYEQIPWRDL QYMFGEVFYG GHITDSMDRR CCTTYLEVLI RNEILPKGNP DEVEAWEAPT LELAPGFFAP K PVDYPTLK EYIETSLPAE SPVVYGMHPN AELSLLTSLG ETLFKTVVEV AGGGGGGGGG GGGGENAVRQ ALETFKERLP EP FNMVEVE LRVKEKTPFV VVALQEATRM NALLSEMKRS MEELQLGLDG ALNMSDNMEK LAKGIASNTV PELWMSCMST RVQ EVYTLT AWYQDVVKRH DQLSAWTAGD IITPHSVWLP GLFNPKAFLT AVMQTFARAN KLPLDVMKFM TEVTRMTSPE QVTE AAPLG VYVHGLVLEG ARWDREDGCL RDSKPNELHP AMPVLQVKPV TADQFNLEGY YECPVYTNMQ RANVYSPVVS TFTLR TQDM PAKWVLASVA LLLQDDLAG

UniProtKB: PLD phosphodiesterase domain-containing protein

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Macromolecule #2: Flagellar outer dynein arm heavy chain beta

MacromoleculeName: Flagellar outer dynein arm heavy chain beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 520.510906 KDa
SequenceString: MAEDEGMTAA APSSSVKDIR FDYIRDRVCS CLKVPDSAYD KLVSGDGRTS LVQFMEEAHT KRLLIMLDGK DLSATVKPPP KFKKKTVYF LKLQETKLDN DNIKKLVIHG EISENPLETL AAISQDVFMP VLTAPANQQG WPDVVAKEVT ENLHKFVSNV F VTIGQMKG ...String:
MAEDEGMTAA APSSSVKDIR FDYIRDRVCS CLKVPDSAYD KLVSGDGRTS LVQFMEEAHT KRLLIMLDGK DLSATVKPPP KFKKKTVYF LKLQETKLDN DNIKKLVIHG EISENPLETL AAISQDVFMP VLTAPANQQG WPDVVAKEVT ENLHKFVSNV F VTIGQMKG QTLLPLPPQN TVPTLQPEQS MHSLKDQDKI HILESAIVTW TKQIKNVLKA DPDAPLKEPG AYPGPLTELN FW SERAANL NSIHEQLTSE KTQKVVKVLE LAKSTYYPAF QRLFREVEAA QQEANDNVKF LKPLRKYLDK LNMMDDFPML VDL FKPIMH TLMLIWKHSK SYNSSTRFVT LMQEICNDLI MQACKYVPGS DLIQMEPSEA VDKLRMTLRV LGTFKNYYFE YRAL SMQDT PENPWKFQNN SLFARLDSFL ERCHDMMDLM STCMQFNRLE RVEIGGTKGK VLTNGVKAIH QDFTSAVEKF QQVTY DVMD VDAKQFDEDF FGFRVVIKEL ERRLAAIIIQ AFDDCTTIGT TFKLLDSFEG LLDREVIAHD LEKKHTDLLH SYARDL KDV ADLFHQYKDR PIVAKNSAPY SGAAYWVRGL MERIKDPMDR LLTMNKMVLE SELFREIQRT YDHLWEEMTE YRTRAVD AW CAQVAATSDE KLNLPLLSLI EETADGIRVL GVNFDPALVR LLRETKYFLL LETSTQDKNA DRNAEKAAEG GEVEVVKK A PKLSVPDSAK DLFASADTFR QQISALDLIC SIYNKVQRTI LAVEKPLVQQ KLDAVEQALN RGLAELNWKC AEIDTYIKE CMELVKDVDL VLNTIKDNVK ATQGILAMWE KNLMFERKDG KTYTFDELND AFNQLIQQRH SEIRDAGKEI TKLLSSSNRV LKVSKGAAS WRAYVDYFSN IVIDGFSAAI ISTVRYLLSQ IDPDILAKTE SSPLLEIQVE LVAPDIVWKP DLGEGGAKPG L RDMIKKWL QSFLEIGQLM KRLDVGEGNY AKELEEDFEV YDALNQVMMV TLANESRCED FKNQFAKFDY LWKQDLQATL QQ FITDNGV TLPDGTRDDP PLAKFEEQIV KYKNVASEIA SFKDTMTMGY VKVNAKPLRQ ALSTWASKWV YLFTHYLQEK VVN SITELY TFMDTSNSTL DLKVMGEGVE EEPEYHPDQD PEEAAAKKAA EEEEKRKALY AIMACMRDIR RRTERGTDTM FEPL KETVT ALHTFGIQLS DTVLHQLDNA EFNWRTLKKK MLNRREQLAP LQQAEAVEIR RKSDAFNERV EDFRTFFQRK APFAV SGGE LKLEQVKPAY KLLDEFRSGS LEGYPSVLGI IAESKQLQEA QDLFELYQPG YLQLQRCSEE LGHLKSLWDT VGTVMF TFR DWYKTPWDKI DVDFLVEETK KLSKDIKMLN KAVRNYDVYR MLEEAIKAVL TSLPLVQDLH HPAMRERHWK LLMQTTG KH FVMDDKFCLG DLLALELHNY VDACSEIVDR AQKELNIEKQ LKKIEDTWAG LSLAFSTYQD SDVMALLVDD AVNEALEA D NLQLQNLSGQ KYVQSNPMFL ETVSKWQNNM GRVSAVLETW QNVQKKWQNL ESIFIGSADI RVQLPEDSKR FDAVNADFQ ELMRTAPDIT NVVEACTLDG RQERLENMQS MLEQCEKALQ EYLETKRVAF PRFYFVSPAD LLDILSKGSN PQLILRHLQK CFDNIDNLS FRKDERGDPT KIATHMHSKE GEVVEFVEDC SCDGPVEVWL QNVVDSMKLA LQVEFRKAIP TYDELPRTKW I YVYSAQNT VVVSRTFFTQ EINEAFDDLE EGNEEALKVE LDRQVQQLAD LIDEINKEQT SLDRKKLITL CTIDVHSRDL VQ KLIDERV EDQMCFQWQS QLRYIQSEKT KTCQVNICDA EIAYSYEYIG NCGCLCITPL TDRCFITLTQ AQRLVLGGAP AGP AGTGKT ETTKDLARAL GIQCYVFNCS DQMDYKAMGH TYKGLAQTGA WGCFDEFNRI PVAVLSVCST QYKTVLDAIR AKKE RFTFE DADISLKSTV MAFITMNPGY PGRAELPESL KALFRPVSMV VPDLALICEI MLMAEGFQMS KILSRKFVIL YKLCE DLLS KSRHYDWKLR AIKTTLYVAG GMKRAAPELS EDKVLLRALR DFNLGKLTAD DTSIFMGLLN DLFPKTLELV PRALDK AFD EAAHKAATEL GYQPDDQFLL KISQLRELFV VRWSVFLLGA AGCGKTAVWR TLLRAQNSSG EKTIYQAVNP KAVTRNE LY GYLHPATREW KEGLMSVTFR NMANNKTNKH QWIVLDGDID AEWIESMNTV MDDNKMLTLA SNERIPLTPS MRLLLEIN H MVHCSPATVS RGGVIFINAD DVGWQPVVAS WIDKLEAAEY RPLLTALFTK YVDPCLEHCR RNFKTVVPLP AVNQAMTIC KILEGILPKE TVRGAPPPDK KLLHYHFVFA CVWAFGGCML VDKVTDYRTQ FSKWWVSEWK DVQFPEKGLV YDYYVDEQNC IMVPWEDRV TKFQYIPGDF TSLFVPTVET TRLTYFLDSL VSNKHYAMFV GNTGTGKSAI MVNKLRNMDT ETMSFYTINM N SLSEAPAL QVILEQPLEK KSGVRYGPPG SRRMVYFVDD MNMPLVDKYD TQSSIELLRQ MVDYHGWYDK VKIQLKEIIN CQ MAACMNP TAGSFNITPR MQRHFVTFAV QMPNAEITRA MYYQIIDGHF SSFDVDVAKM SNKLVDATCE LHRNVMHNFL PSA VKFHYQ FNLRDLSNIT QGLTRAIKEY YREPVKVARL WVHECERVFR DRMINEADMA KFDEFRVAVT KKFFDDCGGM VAIE ERPLI YASHASMTYT PEDVPVYNAL SSYDVLRKTL EDKLREYNES NAVMDLVLFQ QAMEHVTRIA RIIDLPRGNA MLVGV GGSG KQSLARLASY ICGYEVYQIS VSSTYGINDF KENLLGLYRK AGTKGTPITF LMTDNQIVKE GFLVYINDLL STGYIA DLF TPEDKEAFTN AVRNEVKAAG ILDSAENCWD FFIDKVRKFL HIVLCFSPVG DKFRIRARQF PALVNCTMFD WFHGWPG EA LVSVAQRFLV DVPNMEEVVR ENIAYHMAYA HQCVSEASER FKEAFRRYNY TTPKSYLELI SLYKMLLQLK RDDLRRSK E RLENGIDKIA QAAAQVTDLQ RVLKEEQIVV DEKKAQTDEL IVSIGKEKAI VDQAVEAGRE DEEAATALQT EVSAFQAEC ERDLLEAEPI IAQAEAALNS LNKKELSELK SFGSPAAEIV QVAAACLVLT CGGKIPKDRD WNAGKKMMAD VNSFLSSLMN FDKDNVPVV CVEVVEKDYI SNPGFTPDNI KGKSAACAGL CSWVINICKY FRIYQVVAPK RAALAEANKK LDTANKKLKV I RDEVKRLQ DRVALLEQSL MKATEDKNAA IAQADRTARK AQMAERLING LSGENTRWGA EIKRLESLEG RLVGDVLIAS AF VSYAGPF NMQFRKSLVD EKWLPDIIER QIPMTQGIRP LDLLTDDATK AKWANEGLPT DPLSVENGAI MSNASRWALM IDP QLQGIK WIINKETNNG LVIIQQSQPK YIDQVINCIE NGWPLLIENL PVDIDAVLDP VIGKMTIKKG RNIIMKIGDA EVQY DSRFR LYLQTKLSNP HFKPEVAAQT TLVNFCVTEK GLEDQLLALV VDHERPDLQE QAAGLVRSLN EYNITLVELE NNLLF NLAN ATGNILENIE LIEGLEETKR TAVEIEEKVK LAKQTEIQIA KAREVYRPVA TRGSLTYFLI DNLNALDRVY HYSMAN FVF VLKKGMDMTP GGKDESKVPL AERLNQEVDL DKRVELLVET TCFVLIGYVA QGLFERHKLI VATQLCMQIL RSRGELH YA KFEYLLRGPK VMGADNPLHD WVSDSVWGSV QALKELDDYQ GLPEDLIGSS KRWREWMELE RPEDEPLPGD WKRMQEFD K LLLFRALRPD RLTSAMGRFV TNMLGAKYVT SQPYDLERSY QDASPGTPIF VFLSPGVDVA GSVEALGKKL GFTLDNGKY ASVSLGQGQE PIAMDRLSAA HKNGGWVLLQ NIHLTIDWTT NQLDKKVDKL VEGAHPDFRL FLSAEPPPSL ERGLPISLLQ NSIKLTNEP PEGLKANLRR AWNNFNEEIL ESCAKQAEFR AIVFALCYFH AALLERKKFG VGNLPGARSG IGWNMNYPFN T GDLLCCGQ TANNYLENNV KVPWEDLRYN FGEIMYGGHI VEDYDRRLAM CYLRKYVNEG LLDNMEFFPG FAMPPNTANH RQ VLEFIDE VMPPETPLAF GLHPNAEIGF KLREAESFCN SLVQLQPRES SGEGGMSAEE RAKLVLDEVV DKLPDIFDME DVR SKINPD DPNMPFVMVA IQESERMNML LAEMKRSLLE LDLGLKGDLT MTEPMERLLK ALATDAVPGS WRNLAYPSLR PLGS WLGNL LARHAQLVDW TAELSTPKAV WLSGLFNPQS FLTAVMQATA RRNDWPLDKT VIITEVTKKQ PDQIEANSRD GAFIH GLTL EGARWDDKIG ALDDSKPKEL FCPMPVILVR AVTQDKAEMK DVYKCPVYTT EARFREEVFE AQLKSKHTEI KWVLAG VCL FLDVV

UniProtKB: Uncharacterized protein

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Macromolecule #3: Dynein gamma chain, flagellar outer arm

MacromoleculeName: Dynein gamma chain, flagellar outer arm / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 513.491406 KDa
SequenceString: MALDNRHRLI VGKLAEAFGL PENVIEKTLT QDKQAVNSFF TPAGPPSLVF VYQVKEDKLK DGSVGPVDNK PTLHRIGPHE RIHNSVYFT RLNPKGINEK TLEADMGSGE LSVLWALENF KAIVSDLYLP IMQEQQQWGK MSTEYLEDFL SSTAKFGSML T EAVATVSG ...String:
MALDNRHRLI VGKLAEAFGL PENVIEKTLT QDKQAVNSFF TPAGPPSLVF VYQVKEDKLK DGSVGPVDNK PTLHRIGPHE RIHNSVYFT RLNPKGINEK TLEADMGSGE LSVLWALENF KAIVSDLYLP IMQEQQQWGK MSTEYLEDFL SSTAKFGSML T EAVATVSG GVEPMPDPRY IDQYGDLRPA GITQAAGDDD TLQEMEECLT EWCREAELLL NQTNKIKDGE ERGPDTELEY WR TRMSNFN SITEHLKTKE CKLVLGICSH AKTKAYLRWR GLDVQITDAA NESKDNVKYL ATLEKSMEPM YQGRVTDITE SLP ALMTNV RMMYTIARFY STAEHMTRLF TKITNQLVRR CKEQIMENGK IWDQDKVTLI GNMKVSVELA NVYRQQYRLA KETL AAQPK SKQFDFDEQA IFLKFDLSSK ALHKLIDMFT TIHQFSSLEQ HTHIEGLDTM LKSLNNIIDD VKRKPYDLLD YSRNA FDTD FLEFNVQIND LELQLQGFVN ASFEHITSTE HALSLLAQFQ AIMQRETLQQ DLENKYMVIF QNYAKDLDAV QKLYEK NKY EPPVPRNAPP VAGNIMWARQ LLRRIEAPMQ LAQNKNLLAA KESKKNIKTY NKVAKALIEF ETLWHQAWIK SIEQCKA GL AAPLLVQHPD TGKILVNFDK EIMQLVREAK YMQRFNIRCS SPSQMVLLQE EKFKFYHNQL THLVREYEHV LGRGATIK P LLRPHLDDME RKIAPGFAVL TWTSLNIDGY LHRFKQGLAR LEELVRKVVD LTENRVDSNL GAISSTLLVE LPTDRSFTY EGFVEQNRFQ KKQAELLAIR NEEVRRAIED LYTLVRNYPR ENTEDVLDEK EVSLLVRHYS KNMYNAIMQC TLNSLQAMKR RLGSKTTTG IFFMERPFFD VDVELKVPSV CMNPTLEEIQ AAINQCAKKV LTISKQLPAW GMDNVATYHE MMRGDRRWVK A VLRLTGSV EGIKTQVGEY IRTFDKYDFL WKEDLQAAYD HFMRSNPTLE AFEAELKKYM AIETEVTMIN GVNNIGALSL ET HPLKNSL KAEAVSWKTQ FAQNLHKQCS DDLKLDNYIR DTNSKFHRKI EDLEDVRNVM AVLKEVREKE SEIDNLIGPI EEM YGLLMR YEVRVPKEET TMVSDLRYGW KKLKKVATEV SDNLTRLQVG FKRELIKEVK TFVVDAQMFR KDWEANAMVP GLDP QEAVD RLRKFQQMFE VRKRKWENYS SGEELFGLPV TQYPELEQTE KEIQMLDRLY SLYVAVITTI KGYGDYFWVD VVEKI DEMG EQVQQYQNQS KKLPKLRDWP AYNACRKTID DFLEMLPLFQ ALTHKSMRER HWKEVMRVTG HELNLAEDHF KLQHLL DCN VLRYREDIED LTGAAVKEEI IEVKLNQLKA DWATANLALA EYKNRGPVIL KPSDTSELME KLEESQMTLG SMATNRY SA PFRDEVQAWS IKLSTVSEII EQWLMVQSMW QYMEAVFSGG DIVKQLPQEA KRFLNIDKNF MKIVSNALET QNVINTCF G NELMKNMLPH LHEQLEMCQK SLSAYLEQKR AEFPRFTCVG PHLLEICRWA HDPPSVVPHF QSGLFDSLSN VTFDRIDKT RMTEMFSQQN EKVEFERPVD AKGNIEVWLQ RLVDGMEDTV KQIIKRAVRN VAEMPLEDFV FGHPAQVSLL GIQFQWTAET QMALSSAKV DKTIMNKNMK KVDALLRDMV NITVRLDLTK NQRTNLETCI TVHMHQKEST EDLVKKKIKD PTDFEWLKQV R FYWRDDKD TVIISICDVD FEYSFEYLGV KERLVITPLT DICYITLSQA LGMFLGGAPA GPAGTGKTET TKDLGNTLGK YV VVFNCSD QFDYTYMGKI YKGLAQSGLW GCFDEFNRIN LDVLSVCAQQ VYCICRTRER KKSFQFTDGT TVSLDPRVGF FIT MNPGYA GAQELPENLK ALFRGVTMMV PNRQIIMKVK LAAAGYQEND ILSKKFFVLY GLCEQQLSKQ AHYDFGLRNI LSVL RTAGA SKRQSPDKSE VFLMMRTVRD MNMSKFVAED VPLFLSLIDD LFPGLKADAT RPDVNKDAEK VVLERGLQVH PTWMN KCIQ LYETYLVRHG IMLVGPSGSG KSAICECLAA ALTELGTKHV IWRMNPKAIT APQMFGRRDD TTGDWTDGIF AVLWRR AAK NKNQNTWIVL DGPVDAIWIE NLNTVLDDNK VLTLANGDRI LMSAAMKAMF EPENLNNASP ATVSRAGIIY VSDVELG WE PPVKSWLQKR DPTEACWARL FSKYIDRMLE FVRISLKPVM YNEQVSIVGT VMTLLNGYLK SMKEAGTAMN DAKYERVF L YCMTWSLGGL LEMKERPLFD QELRTFAHNM PPKEEDSDTI FEFLVNTTDA EWLHWRHCVP VWTYPKNEEK PQYAQLVIP TLDSVRYGAL LNLSYNVDKA TLLVGGPGTA KTNTINQFIS KFNAETTANK TITFSSLTTP GIFQMSIEGA VEKRQGRTFG PPGGKQMCI FVDDISMPYI NEWGHQVTNE IVRQLLEQGG MYSLEKPIGD MKFITDVRYV AAMNTPGGGK NDIPNRLKRQ F AIFNVPLP SVAAINGIFG KLVEGRFSRD VFCEEVVYVA SKLVPLTITL WNRIQTKMLP TPAKFHYLFN MRELSKVFQG VI LATRDRF NLAAGDSAVF GGNVASPEGY LLGLWIHECR RVFSDKLISY EDKNWVDKAV FDLCRDNFSS DLVKQVEEPI YFV DFLREP AVMMRPVEIV TPHPSFYYSV PGGLPEVRAR VEGLQRKFNE ESKVMKLELV LFTDCVTHLM RITRLLAWPG LGLL VGVGG SGKQSLSRLS AYIAGPTFYI TKTYNVSNLF EHIKGLYKIA GFKGQPVYFI FTDAEVKDEG FLEYINQILM TGEVA GLLT KEDQDMIVND IRPVMKHQAP GILDTYDNLY NFFLNRVRDN LHVVLCFSPV GAKFARRAQQ FPGLINGCTI DWFCPG PKK RLTSVSGKFI DKFTMACPKE VKNQLELLMG HAHVFVTAAC KEYFEKYRRY VYVTPKSYLS FLQGYKELYA KKWSFTK EL AYQIEVACQK MFEPKADVNK MKAELAVKNQ TAVSAKEAEA LLKQISESTA IAEKEKQKVA VIVDAVTKKA SEIATVKD D AERDLAAAKP ALDAALEALN SIKDGDIKNL KALKKPPQII TRIFDCVLVL RMLPVTKAEY TDEKGRMVQV GNYPEAQKM MNQMSFLQDL KDFAKEQIND ETVELLEPYF MSEDFTFENA QKGSGNVAGL CNWAESMAKY HNVAKVVEPK IAKLREAEAE LKLATKEKN AAEERMAKVQ AKLDEMQAQF DAAMAHKQAL EDDAAATQRK MDSANALIGA LAGEEARWTA QSKEFDVQIQ R LTGDCALA SAFVSYLGPF NKEFRELLLN RDFYGDCMKL NVPVTPHLQI TKFLVDDSEV GEWNLQGLPT DELSIQNGIM VT RASRYPV LVDPQGQGRE WIKNREEANQ LKTTQLNDKL FRNHLEECLA FGRPLLIENI EEELDPLLDP VLERRLVKKG KTW VVPLAD KEVDFTETFR LFCTTRLPNP HFTPELSAKV TVVDFTVTMA GLEDQLLGKL ISKEKKELED QRQQLLEEVQ SYKK RIKQL EDDLLCRLSN SQGNLLDEHQ ELIDVLAVTK QTAQDVSEKL ANASETNKRI NEACEEYRPV AHRATLLYFL IAEFS VVNC MYQTSLAQFN QLYELAIDRS EKANMPSKRI HNIIEYMTYE IYLYVQRGLF ERHKIIFALM LTNKVLTSAG KVKATD LDV FLKGGAALDI NSVRKKPKDW IPDLVWLNII ALSAMDAFRD IPDSVFRNDG LWRQWYDQEA PEMAKVPDYE DRLNKFE RM CVVKTFREDR TLIAAADYIA EALGQRFVES VPLNMEKRPG RRAMAKCPLI CLLSPGPDPT KLIEDLAKKK KIKTLGVS M GQGQEVIARK HMAAASLEGH WVLLQNTHLG LGYLTEVETF LVKEENVHED FRLWITAEPH PQFPIGLLQM GIKITNEAP VGIKAGLRAS YQWVNQDMLD MVSRQEWRQL LFVMCFLHSV VQEPQFGPIG WNVPYEFNQS DLSACVQFLQ NHLSEMDAKK APQPTWETV RYMISAIQYG SRITDDFDKL LMDTFAEKYF LQPVLQPSYE LFKDTRSSDG FSYRVPDSTD IETFGSYIET L PGTESPEI FGLHPNADIT FRTLQVQESI VTILDTMPKG AGSGSGLSRE DVVDKICEDL LSKAPPLFDK EETKEKLKKL PG GPTLPLT VHLRQEIDRL NIVTRLTTTT LKNLALAIAG TIAAERGLID ALDALFNARI PQQWLSKSWE ASTLGNWFTG LLQ RYDQLN KWLNLGRPKA YWMTGFFNPQ GFLTAMKQEV NRKHRDKWAL DDVVMSSEVT HRPKDFESLK EGAPEGVYVY GLYL DLRLD GRENRLMDSD PKKLFNPLPV LHVDGVLAKD KKRSGLYEAP KPYRVKARKG LNFITTFSVR TEDDKSKWIL PGVGI LCSI D

UniProtKB: Dynein gamma chain, flagellar outer arm

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Macromolecule #4: Dynein, 78 kDa intermediate chain, flagellar outer arm

MacromoleculeName: Dynein, 78 kDa intermediate chain, flagellar outer arm
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 76.62868 KDa
SequenceString: MPALSPAKKG TDKGKTGKKT GKQEQNAQDY IPPPPPMPGD EAFAMPIREI VKPDNQLWLS EADLNEEVAK MLTANNPAAP KNIVRFNMK DKVFKLEPMV EQTVVHYATD GWLLHKSSDE AKRQMDMEKM EQEASARFQA DIDRASHEHK DHGDVEPPDD S RQLRNQFN ...String:
MPALSPAKKG TDKGKTGKKT GKQEQNAQDY IPPPPPMPGD EAFAMPIREI VKPDNQLWLS EADLNEEVAK MLTANNPAAP KNIVRFNMK DKVFKLEPMV EQTVVHYATD GWLLHKSSDE AKRQMDMEKM EQEASARFQA DIDRASHEHK DHGDVEPPDD S RQLRNQFN FSERAAQTLN YPLRDRETFT EPPPTATVSG ACTQWEIYDE YIKDLERQRI DEAMKSKGGK KAAAAARAAG AA HRQRNEH VPTLQSPTLM HSLGTLDRMV NQNMYEEVAM DFKYWDDASD AFRPGEGSLL PLWRFVSDKS KRRQVTSVCW NPL YDDMFA VGYGSYEFLK QASGLINIYS LKNPSHPEYT FHTESGVMCV HFHPEFANLL AVGCYDGSVL VYDVRLKKDE PIYQ ASVRT GKLNDPVWQI YWQPDDAQKS LQFVSISSDG AVNLWTLTKS ELIPECLMKL RVVRAGETRE EEDPNASGAA GGCCM DFCK MPGQESIYLV GTEEGAIHRC SKAYSSQYLS TYVSHHLAVY AVHWNNIHPS MFLSASCRLD HQAVGLCHDP KRAVMN FDL NDSIGDVSWA ALQPTVFAAV TDDGRVHVFD LAQNKLLPLC SQKVVKKAKL TKLVFNPKHP IVLVGDDKGC VTSLKLS PN LRITSKPEKG QKFEDLEVAK LDGVVEIARK SDADLAKNAA H

UniProtKB: Dynein, 78 kDa intermediate chain, flagellar outer arm

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Macromolecule #5: Dynein, 70 kDa intermediate chain, flagellar outer arm

MacromoleculeName: Dynein, 70 kDa intermediate chain, flagellar outer arm
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 63.594062 KDa
SequenceString: MEIYHQYIKL RKQFGRFPKF GDEGSEMLAD IRPNEDHGKE YIPRNPVTTV TQCVPEMSEH EANTNAVILV NKAMSHVEGG WPKDVDYTE AEHTIRYRKK VEKDEDYIRT VVQLGSSVED LIKQNNAVDI YQEYFTNVTM DHTSEAPHVK TVTVFKDPNN I KRSASYVN ...String:
MEIYHQYIKL RKQFGRFPKF GDEGSEMLAD IRPNEDHGKE YIPRNPVTTV TQCVPEMSEH EANTNAVILV NKAMSHVEGG WPKDVDYTE AEHTIRYRKK VEKDEDYIRT VVQLGSSVED LIKQNNAVDI YQEYFTNVTM DHTSEAPHVK TVTVFKDPNN I KRSASYVN WHPDGSVPKV VVAYSILQFQ QQPAGMPLSS YIWDVNNPNT PEYEMVPTSQ ICCAKFNLKD NNLVGAGQYN GQ LAYFDVR KGNGPVEATP IDISHRDPIY DFAWLQSKTG TECMTVSTDG NVLWWDLRKM NECVENMPLK EKNSETTVGG VCL EYDTNA GPTNFMVGTE QGQIFSCNRK AKNPVDRVKY VLSGHHGPIY GLRRNPFNSK YFLSIGDWTA RVWVEDTAVK TPIL TTKYH PTYLTGGTWS PSRPGVFFTI KMDGAMDVWD LYYKHNEPTL TVQVSDLALT AFAVQESGGT VAVGTSDGCT SVLQL STGL SEASPAEKAN INAMFERETT REKNLEKAIK EAKVKARKEQ GRRDEVKDNV TEEQLKALED EFFKTTDPAV GGGYGA GEG AAAE

UniProtKB: Dynein, 70 kDa intermediate chain, flagellar outer arm

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Macromolecule #6: Flagellar outer dynein arm light chain 2

MacromoleculeName: Flagellar outer dynein arm light chain 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.903028 KDa
SequenceString:
MDDMPFQSGG IKEVVYENTF ITDPEGYGPN TKFERHKVQA VLKQVLKERI EKQKYDPVKG AQISKQLADD LREKVKALGY DRYKLVIQV TVGQKQGQAM RIISRCLWDQ TNDNFASEYY ENESMYCVCQ VYGLYYE

UniProtKB: Mr19,000 outer arm dynein light chain

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Macromolecule #7: Dynein 18 kDa light chain, flagellar outer arm

MacromoleculeName: Dynein 18 kDa light chain, flagellar outer arm / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 17.807119 KDa
SequenceString:
MAAKVDIFAA NLPNLTDEEM DMCRKAFAMF DKDGSGTIDT KELRTALSAL GQNPTEEDMF VMISQVDQDG SRCIEFKEFV RVIQINKQM SAKDADEADT LDAFVALGGN LDKTGRILVD KLRSICEEFE LTVNVDRLVK DADRDLNGFL SYDEFRALLS

UniProtKB: Dynein 18 kDa light chain, flagellar outer arm

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Macromolecule #8: Dynein 11 kDa light chain, flagellar outer arm

MacromoleculeName: Dynein 11 kDa light chain, flagellar outer arm / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.876627 KDa
SequenceString:
MADEKEQQGE QGYQLQSQHF YKIITSYMPE SFEQDAVQIA LIAVDKYRQL KDIAFYIKHE YDKKYPGSGK ATEGVYHCIV GKSFASAVS HETRQFIHMK VDTYHVILWK SKDTPFTPGG E

UniProtKB: Dynein 11 kDa light chain, flagellar outer arm

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Macromolecule #9: Dynein light chain roadblock LC7a

MacromoleculeName: Dynein light chain roadblock LC7a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 11.946708 KDa
SequenceString:
MVDIAAVDET FKRLQSHKGV LGIIVINAEG IAIRTTFDND LTVQYAALVS HFTVKARSAV RKLDGDNDLK FLRIRSKKHE IMIAPEFER SHEYYLVVVQ DPSREA

UniProtKB: Dynein light chain roadblock

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Macromolecule #10: Dynein light chain roadblock LC7b

MacromoleculeName: Dynein light chain roadblock LC7b / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 11.132679 KDa
SequenceString:
MSDIESTLTR IQGHKGVIGV IIVNNQGVPL RSTFEHDAMT KQYADLVPGL ADLARNLVRD LDPQNDLEFL RIRSHKHEIM VAAKDDFVL LVIQDPNAAS T

UniProtKB: Dynein light chain roadblock

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Macromolecule #11: Dynein 8 kDa light chain, flagellar outer arm

MacromoleculeName: Dynein 8 kDa light chain, flagellar outer arm / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.336775 KDa
SequenceString:
MASGSSKAVI KNADMSEEMQ ADAVDCATQA LEKYNIEKDI AAYIKKEFDR KHNPTWHCIV GRNFGSYVTH ETKHFIYFYL GQVAILLFK SG

UniProtKB: Dynein 8 kDa light chain, flagellar outer arm

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Macromolecule #12: Dynein light chain 9

MacromoleculeName: Dynein light chain 9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.993606 KDa
SequenceString:
MEDDTAFDGL AEDDLMDDAE VDTMVRESIV AAIGDNQFAH NKIDSWSNNI VEGCLKRLAA MNKPFKYIVT TNLTQKAGAG LHAASCARW NEKTDGKLTV QWENNTMIVL VSVYWLAV

UniProtKB: Dynein light chain 9

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Macromolecule #13: Dynein light chain 10

MacromoleculeName: Dynein light chain 10 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.101117 KDa
SequenceString:
MAEQQFEDME AFLRVAKYTL VKFTDMHVEM KEEAMDICIT AVEKYPNDAE KCTQMIKDQM DKKFGAPWHV VVGKGFSYEI TYEVRNLLY IYVGGRTAVL LWKM

UniProtKB: Dynein light chain

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Macromolecule #14: DC1

MacromoleculeName: DC1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.315707 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #15: DC2

MacromoleculeName: DC2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 14.31563 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #16: Outer dynein arm-docking complex protein DC3

MacromoleculeName: Outer dynein arm-docking complex protein DC3 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.371311 KDa
SequenceString:
MASAAARERY ARKNEQNELE RAFNTIDSKG DKKIDAEELT ELFLRLGHKP KRGEVEDMIW EVDEDCDGCI SLLEFQTLYT RCRDDKTGY EPRGLFNVVE FVMNDKNNQG FITLEEAMQI MYLRYGRAEL DMQLEQVFGT ADLNSGKILT LTEFLHCLHT N QVKQLLNR VTAKTYKAPP PPQKRK

UniProtKB: Outer dynein arm-docking complex protein DC3

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statefilament

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Sample preparation

Concentration20 mg/mL
BufferpH: 7.4
Component:
ConcentrationNameFormula
30.0 mMHEPES
25.0 mMKCl
5.0 mMMgSO4
0.5 mMEGTA
10.0 mMATPAdenosine triphosphate
0.75 mMCaCl2

Details: Buffer also contained 1x Protease Arrest (G-Biosciences)
GridModel: C-flat-1.2/1.3 / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Details: 15 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV
Details: 2.5 ul of splayed axoneme solution was then dispensed onto glow-discharged C-Flat 1.2/1.3-4Cu grids inside a Vitrobot Mark IV under 100% humidity. After a 10 s delay time, cryo-EM samples ...Details: 2.5 ul of splayed axoneme solution was then dispensed onto glow-discharged C-Flat 1.2/1.3-4Cu grids inside a Vitrobot Mark IV under 100% humidity. After a 10 s delay time, cryo-EM samples were prepared by first blotting for 10 s with blot force set to 16 and immediately plunged into liquid ethane..
DetailsSplayed axonemes isolated from Chlamydomonas reinhardtii flagella.

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Electron microscopy

MicroscopeTFS KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 25 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 2 / Number real images: 20524 / Average exposure time: 3.7 sec. / Average electron dose: 61.48 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Segment selectionNumber selected: 5584147 / Software - Name: RELION (ver. 3.1)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6u42

Final angle assignmentType: NOT APPLICABLE / Software - Name: RELION (ver. 3.1)
Final reconstructionApplied symmetry - Helical parameters - Δz: 82.0 Å
Applied symmetry - Helical parameters - Δ&Phi: 0 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1)
Details: The composite map was generated from three focused refinements of the full ODA core map. The three focused refinements centered on IC1-LC7a/b (3.6 A resolution), IC2 (3.5 A resolution), and ...Details: The composite map was generated from three focused refinements of the full ODA core map. The three focused refinements centered on IC1-LC7a/b (3.6 A resolution), IC2 (3.5 A resolution), and the LC8s (4.0 A resolution).
Number images used: 485694

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER / Target criteria: Correlation coefficient
Output model

PDB-7kzn:
Outer dynein arm core subcomplex from C. reinhardtii

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