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Yorodumi- EMDB-17036: Double-headed nucleocapsid of the human Respiratory Syncytial Virus -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-17036 | |||||||||
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Title | Double-headed nucleocapsid of the human Respiratory Syncytial Virus | |||||||||
Map data | Double-headed nucleocapsid of the Respiratory Syncytial Virus. | |||||||||
Sample |
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Keywords | spiral clam / N-RNA / nucleoprotein / nucleocapsid / VIRAL PROTEIN | |||||||||
Biological species | Human respiratory syncytial virus A strain Long | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
Authors | Gonnin L / Desfosses A / Gutsche I | |||||||||
Funding support | France, 1 items
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Citation | Journal: Nat Commun / Year: 2023 Title: Structural landscape of the respiratory syncytial virus nucleocapsids. Authors: Lorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche / Abstract: Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_17036.map.gz | 13.8 MB | EMDB map data format | |
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Header (meta data) | emd-17036-v30.xml emd-17036.xml | 13.7 KB 13.7 KB | Display Display | EMDB header |
Images | emd_17036.png | 154.4 KB | ||
Others | emd_17036_half_map_1.map.gz emd_17036_half_map_2.map.gz | 301.2 MB 301.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-17036 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17036 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_17036.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Double-headed nucleocapsid of the Respiratory Syncytial Virus. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.145 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Double-headed nucleocapsid of the Respiratory Syncytial Virus. Half...
File | emd_17036_half_map_1.map | ||||||||||||
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Annotation | Double-headed nucleocapsid of the Respiratory Syncytial Virus. Half B. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Double-headed nucleocapsid of the Respiratory Syncytial Virus. Half...
File | emd_17036_half_map_2.map | ||||||||||||
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Annotation | Double-headed nucleocapsid of the Respiratory Syncytial Virus. Half A. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Double-headed nucleocapsid of the human Respiratory Syncytial Virus
Entire | Name: Double-headed nucleocapsid of the human Respiratory Syncytial Virus |
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Components |
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-Supramolecule #1: Double-headed nucleocapsid of the human Respiratory Syncytial Virus
Supramolecule | Name: Double-headed nucleocapsid of the human Respiratory Syncytial Virus type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 Details: Double-headed nucleocapsid formed by the nucleoprotein N of the human RSV upon overexpression in insect cells and encapsidation of cellular RNA |
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Source (natural) | Organism: Human respiratory syncytial virus A strain Long |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS GLACIOS |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.7000000000000001 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 42.0 e/Å2 |
-Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 25338 |