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- EMDB-13552: Assembly intermediate of mouse mitochondrial ribosome small subun... -

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Basic information

Entry
Database: EMDB / ID: EMD-13552
TitleAssembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA inward conformation
Map dataOversampled combined map of local-masked refined maps with sharpening and local-resolution filtering
Sample
  • Complex: Assembly intermediate of small subunit of human mitochondrial ribosome
    • RNA: x 1 types
    • Protein or peptide: x 30 types
  • Ligand: x 6 types
KeywordsAssembly intermediate / maturation / biogenesis / small subunit / mitochondrion / RIBOSOME
Function / homology
Function and homology information


Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial ribosome binding / anaphase-promoting complex / mitochondrial ribosome assembly / positive regulation of mitochondrial translation / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / apoptotic mitochondrial changes ...Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial ribosome binding / anaphase-promoting complex / mitochondrial ribosome assembly / positive regulation of mitochondrial translation / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / apoptotic mitochondrial changes / positive regulation of proteolysis / ribosomal small subunit binding / rRNA processing / ribosomal small subunit assembly / small ribosomal subunit rRNA binding / regulation of translation / cell junction / nuclear membrane / cell population proliferation / tRNA binding / mitochondrial inner membrane / rRNA binding / protein ubiquitination / ribosome / mitochondrial matrix / structural constituent of ribosome / translation / protein domain specific binding / intracellular membrane-bounded organelle / mRNA binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Putative ribosome-binding factor A, mitochondrial / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 ...Putative ribosome-binding factor A, mitochondrial / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / 40S ribosomal protein rpS2 (S5p), N-terminal / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S10 / Ribosomal protein S2 signature 1. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S14 / Ribosomal protein S2 / K homology domain-like, alpha/beta / Ribosomal protein S14p/S29e / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal S11, conserved site / Ribosomal protein S10p/S20e / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / Ribosomal protein S11 / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / Ribosomal protein S10p/S20e / Ribosomal protein S9 / Ribosomal protein S11 / Ribosomal protein S17/S11 / Ribosomal_S15 / Ribosomal protein S15 / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e / Ribosomal protein S9/S16
Similarity search - Domain/homology
Small ribosomal subunit protein uS12m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS31 / Putative ribosome-binding factor A, mitochondrial / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS35 ...Small ribosomal subunit protein uS12m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS31 / Putative ribosome-binding factor A, mitochondrial / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein mS40 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS34
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.06 Å
AuthorsItoh Y / Khawaja A
Funding supportEuropean Union, 3 items
OrganizationGrant numberCountry
European Research Council (ERC)ERC-2018-StG-805230European Union
The Swedish Foundation for Strategic ResearchFFL15:0325European Union
European Commission799399-ItohriboEuropean Union
CitationJournal: Nature / Year: 2022
Title: Mechanism of mitoribosomal small subunit biogenesis and preinitiation.
Authors: Yuzuru Itoh / Anas Khawaja / Ivan Laptev / Miriam Cipullo / Ilian Atanassov / Petr Sergiev / Joanna Rorbach / Alexey Amunts /
Abstract: Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) ...Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. ) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.
History
DepositionSep 8, 2021-
Header (metadata) releaseJun 15, 2022-
Map releaseJun 15, 2022-
UpdateJan 17, 2024-
Current statusJan 17, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13552.png

Downloads & links

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Map

FileDownload / File: emd_13552.map.gz / Format: CCP4 / Size: 729 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationOversampled combined map of local-masked refined maps with sharpening and local-resolution filtering
Voxel sizeX=Y=Z: 0.69167 Å
Density
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-1.000616 - 1.7548081
Average (Standard dev.)0.000000000003873 (±0.032463536)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderYXZ
Origin000
Dimensions576576576
Spacing576576576
CellA=B=C: 398.40192 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_13552_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Combined map of local-masked refined maps without sharpening

Fileemd_13552_additional_1.map
AnnotationCombined map of local-masked refined maps without sharpening
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of the overall refinement

Fileemd_13552_half_map_1.map
AnnotationHalf map of the overall refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of the overall refinement

Fileemd_13552_half_map_2.map
AnnotationHalf map of the overall refinement
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Assembly intermediate of small subunit of human mitochondrial ribosome

EntireName: Assembly intermediate of small subunit of human mitochondrial ribosome
Components
  • Complex: Assembly intermediate of small subunit of human mitochondrial ribosome
    • RNA: 12S mitochondrial rRNA
    • Protein or peptide: 28S ribosomal protein S2, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S24, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S5, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S6, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S7, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S9, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S10, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S11, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S12, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S14, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S15, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S17, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S18c, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S21, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S22, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S23, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S25, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S26, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S27, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S28, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S29, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S31, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S33, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S34, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S35, mitochondrialRibosome
    • Protein or peptide: Aurora kinase A-interacting protein
    • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Protein or peptide: Putative ribosome-binding factor A, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM IONPotassium
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: water

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Supramolecule #1: Assembly intermediate of small subunit of human mitochondrial ribosome

SupramoleculeName: Assembly intermediate of small subunit of human mitochondrial ribosome
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#31
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: 12S mitochondrial rRNA

MacromoleculeName: 12S mitochondrial rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 306.793344 KDa
SequenceString: AAAGGUUUGG UCCUGGCCUU AUAAUUAAUU AGAGGUAAAA UUACACAUGC AAACCUCCAU AGACCGGUGU AAAAUCCCUU AAACAUUUA CUUAAAAUUU AAGGAGAGGG UAUCAAGCAC AUUAAAAUAG CUUAAGACAC CUUGCCUAGC CACACCCCCA C GGGACUCA ...String:
AAAGGUUUGG UCCUGGCCUU AUAAUUAAUU AGAGGUAAAA UUACACAUGC AAACCUCCAU AGACCGGUGU AAAAUCCCUU AAACAUUUA CUUAAAAUUU AAGGAGAGGG UAUCAAGCAC AUUAAAAUAG CUUAAGACAC CUUGCCUAGC CACACCCCCA C GGGACUCA GCAGUGAUAA AUAUUAAGCA AUAAACGAAA GUUUGACUAA GUUAUACCUC UUAGGGUUGG UAAAUUUCGU GC CAGCCAC CGCGGUCAUA CGAUUAACCC AAACUAAUUA UCUUCGGCGU AAAACGUGUC AACUAUAAAU AAAUAAAUAG AAU UAAAAU CCAACUUAUA UGUGAAAAUU CAUUGUUAGG ACCUAAACUC AAUAACGAAA GUAAUUCUAG UCAUUUAUAA UACA CGACA GCUAAGACCC AAACUGGGAU UAGAUACCCC ACUAUGCUUA GCCAUAAACC UAAAUAAUUA AAUUUAACAA AACUA UUUG CCAGAGAACU ACUAGCCAUA GCUUAAAACU CAAAGGACUU GGCGGUACUU UAUAUCCAUC UAGAGGAGCC UGUUCU AUA AUCGAUAAAC CCCGCUCUAC CUCACCAUCU CUUGCUAAUU CAGCCUAUAU ACCGCCAUCU UCAGCAAACC CUAAAAA GG UAUUAAAGUA AGCAAAAGAA UCAAACAUAA AAACGUUAGG UCAAGGUGUA GCCAAUGAAA UGGGAAGAAA UGGGCUAC A UUUUCUUAUA AAAGAACAUU ACUAUACCCU UUAUGAAACU AAAGGACUAA GGAGGAUUUA GUAGUAAAUU AAGAAUAGA GAGCUUAAUU GAAUUGAGCA AUGAAGUACG CACACACCGC CCGUCACCCU CCUCAAAUUA AAUUAAACUU AACAUAAUUA AUUUCUAGA CAUCCGUUUA UGAGAGGAGA UAAGUCGUAA CAAGGUAAGC AUACUGGAAA GUGUGCUUGG AAUAAUC

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Macromolecule #2: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 32.35715 KDa
SequenceString: MAPAPAVLTR LLCAGVRRWP GFLQKAIPGP AEQNGRKVTG APVPAVSEPQ DGDDFQSRIL DTPLQHSDFF NVKELFSVKS LFEARVHLG HKAGCRHRFM EPYIFGNRLG QDIIDLDQTA LNLQLALNFT AHVAYRKGII LFVSRNRQFS HLIETTAQAC G EYAHTRYF ...String:
MAPAPAVLTR LLCAGVRRWP GFLQKAIPGP AEQNGRKVTG APVPAVSEPQ DGDDFQSRIL DTPLQHSDFF NVKELFSVKS LFEARVHLG HKAGCRHRFM EPYIFGNRLG QDIIDLDQTA LNLQLALNFT AHVAYRKGII LFVSRNRQFS HLIETTAQAC G EYAHTRYF KGGLLTNAQL LFGPSVRLPD LIIFLHTLNN VFEPHVAVRD AAKMNIPTVG IVDTNCNPCL ITYPIPGNDD SP QAIQLFC KLFRTTINRA KEKRRQMEAL HRLQSPKGSE GSGTSPVPDK SHSP

UniProtKB: Small ribosomal subunit protein uS2m

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Macromolecule #3: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 18.934086 KDa
SequenceString:
MAWSASVRGL GQRVLACSRE LPGAWRTLHT SAVCAKNRAA RVRVAKGNKP VSYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTLED VFLRKFMMGT FPGCLADQIV LKRRANQVDI CALVLRQLPA HKFYFLVGYS ETLLSHFYKC PVRLHLQTVP S KVVYKYI

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #4: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.293598 KDa
SequenceString: MAAAVRAAGC LPALCSLQAG HFLSRQLSLN AFPVAATSFL AVKTALSHGS LSSRETRRNH CLTSLSHVLQ TQCCVSSPGN WTGQQCRPY SFFTKLTAEE LWKGALAETG AGARKGRGKR TKKKKRKDLN RGQIIGEGRS GFLWPGLNVP LIKSGVVQNI G QRSKEEQQ ...String:
MAAAVRAAGC LPALCSLQAG HFLSRQLSLN AFPVAATSFL AVKTALSHGS LSSRETRRNH CLTSLSHVLQ TQCCVSSPGN WTGQQCRPY SFFTKLTAEE LWKGALAETG AGARKGRGKR TKKKKRKDLN RGQIIGEGRS GFLWPGLNVP LIKSGVVQNI G QRSKEEQQ KVEATMVEQR EEWDRKRKIK VKRERGWSGN TWGGVSIGPP DPGPNGETYE DFDTRILEVR NVFNMTAKEG RK KSVRVLV AVGNGNGAAG FAIGKAADRG DAFRKAKNRA IHYLHYIERY EGHTIFHDIS LRFKRTQIRM KKQPRGYGLR CHR AIITIC RLIGIKDMYA RVTGSMNMLN LTRGLFHGLA RQETHQHLAD KKGLHVVEFR EECGPLPIVV ASPHGALSKE PEPE PEVPD TKLDWQDVKA MQGLKRSVWF NLKRPAT

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #5: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 14.333639 KDa
SequenceString:
MPRYELALIL KAMRRPETAA ALKRTIESLM DRGAIVRNLE SLGERALPYR ISSHSQQHSR GGYFLVDFYA PTSAVENILE HLARDIDVV RPNIVKHPLT QEVKECDGIV PVPLEEKLYS TKRRKK

UniProtKB: Small ribosomal subunit protein bS6m

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Macromolecule #6: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 28.112869 KDa
SequenceString: MAAPALRAPL RWSGLALGVR CAVWNLPGLT QVRGSRYAPE FREPLIDKEY YRKPVAELTE EEKYDQELKK TQFIKAAAAT ETSSVFADP VISKFTNMMM KGGNKVLARS LMAQTLEAVK RKQFEKYRAA SAEEQATIER NPYRIFHEAL KNCEPVIGLV P ILKGGHFY ...String:
MAAPALRAPL RWSGLALGVR CAVWNLPGLT QVRGSRYAPE FREPLIDKEY YRKPVAELTE EEKYDQELKK TQFIKAAAAT ETSSVFADP VISKFTNMMM KGGNKVLARS LMAQTLEAVK RKQFEKYRAA SAEEQATIER NPYRIFHEAL KNCEPVIGLV P ILKGGHFY QVPVPLADRR RRFLAMKWMI TECRENKPRR TLMPEKLSHE LLEAFHNRGP VIKRKHNMHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #7: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 44.997406 KDa
SequenceString: MAAPCVSCGR VLSLWFTPAV RASLCQRPGY WTASAVGWQT GTRFQLSKLI HTTVVTTKKN VQASRQESYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF AEEDIDRAIA YLFPSGLFEK RARPMMKHPE HIFPKQRATQ WGEDGRPFHF LFYTGKQSYY S LMHDVYGK ...String:
MAAPCVSCGR VLSLWFTPAV RASLCQRPGY WTASAVGWQT GTRFQLSKLI HTTVVTTKKN VQASRQESYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF AEEDIDRAIA YLFPSGLFEK RARPMMKHPE HIFPKQRATQ WGEDGRPFHF LFYTGKQSYY S LMHDVYGK VMQLEKHRGP LSASAESRDL IGSRWLIKEE LEEMLVEKLS DEDYAQFIRL LEKLLTLPCG PAEEEFVQRF RR SVTIQSK KQLIEPVQYD EQGMAFSTSE GRRKSATAQA VVYEHGSGKI HVNGVDYLIY FPITQDREQL MFPFHFLDRL ERH DVTCTV SGGGRSAQAG AIRLAMARAL CSFVTEDEVE WMRQAGLLTP DPRIRERKKP GQEGARRKFT WKKR

UniProtKB: Small ribosomal subunit protein uS9m

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Macromolecule #8: 28S ribosomal protein S10, mitochondrial

MacromoleculeName: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 18.729764 KDa
SequenceString:
MKWVPLSNLH VDVPKDVTRP TITTSDEPDT LYKRLSILVK AHDRAVLDSY EYFAVLAAKE LGISIKVHEP PRKIERFTLL KSVHIFKKH RVQYEMRTLY RCLELKHLTG STASVYLEYI QRNLPEGVAM EVTKTQIQQL PEHIKEPMWE TVPEEKKESK S

UniProtKB: Small ribosomal subunit protein uS10m

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Macromolecule #9: 28S ribosomal protein S11, mitochondrial

MacromoleculeName: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.252193 KDa
SequenceString: MQVLRNSGSW LLLSWAGHGM TRFVGIAPAK NIHTGAPRLE DSAARQNTER EAAPSRFSLY PPVPGQESSL QWAGMKFEDV PIAHIKATY NNTQIQVVSA TNASLARASC GTEGFRNAKK GTGIAAQTAG IAAAAKATGK GVTHIRVVVK GMGPGRWSAI K GLTMGGLE ...String:
MQVLRNSGSW LLLSWAGHGM TRFVGIAPAK NIHTGAPRLE DSAARQNTER EAAPSRFSLY PPVPGQESSL QWAGMKFEDV PIAHIKATY NNTQIQVVSA TNASLARASC GTEGFRNAKK GTGIAAQTAG IAAAAKATGK GVTHIRVVVK GMGPGRWSAI K GLTMGGLE VISITDNTPV PH(5F0)GCRPRKA RRL

UniProtKB: Small ribosomal subunit protein uS11m

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Macromolecule #10: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.469326 KDa
SequenceString:
MSWPGLLYGL TTSLSRGLAL APQLWAARSM ATLNQMHRLG PRKEPPKRLG PTEGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGK EAVCFIPGEG HTLQEHHVVL VEGGRTQDLP GVKLKVVRGK YDCGHVQKKK

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #11: 28S ribosomal protein S14, mitochondrial

MacromoleculeName: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 14.949552 KDa
SequenceString:
MAASVLGSLL RTFRQAVPPS ASGQVRGYYV DWRMLRDLKR RKMAYEYADE RLRINSLRKN TILPKDLQEM AGDEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGL LSGVQRAIW

UniProtKB: Small ribosomal subunit protein uS14m

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Macromolecule #12: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 29.523672 KDa
SequenceString: MLRAAWRALS SVRAQAVTRA PVPALRGGSS ASLLSARCGL QPPSLLRAAR AYAAVQKPVQ PKQDDEPPSS AFIKEYKDII PNIEKVDDV VKRILSLEMA SRKEKLKIKQ EQLMNKIVEN PEDSRTLEAQ IIALTVRIRN YEEHMQKHRK DKAHKRHLLM S IDRRKKLL ...String:
MLRAAWRALS SVRAQAVTRA PVPALRGGSS ASLLSARCGL QPPSLLRAAR AYAAVQKPVQ PKQDDEPPSS AFIKEYKDII PNIEKVDDV VKRILSLEMA SRKEKLKIKQ EQLMNKIVEN PEDSRTLEAQ IIALTVRIRN YEEHMQKHRK DKAHKRHLLM S IDRRKKLL KILRQTNYDV FEKTCKELGV EYTLPPLHFQ KVHRRFLAKK ALCIRVYQEV QKLKKQKRAL KAAAAAAKKE KN EGVPENP SNAVPEKTQV N

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #13: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.218721 KDa
SequenceString:
MVQLTTIFCK AYHGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLSGFFPL HPMMITNAER LRRRRAREVL LASQKAESEA KETEAS

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #14: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 13.405907 KDa
SequenceString:
MSIVRSSVHA KWVVGKVIGT AMIKTAKVRA TRLVLDPYLL KYFNKRKTYF AHDALQQCSV GDIVLLRALP VPRSKHVKHE LAEIIFKVG RVIDPVTGKP CAGTAYLESP LSEPREELKV S

UniProtKB: Small ribosomal subunit protein uS17m

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Macromolecule #15: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 28.740689 KDa
SequenceString: MAAPLRHTLL KLVPTLLRSS YVAQVPLQTL CTRGPPEEDA PSSLPVSPYE SEPWKYLDSE EYHNRYGSRP VWADYRRNHK GGVPPQRTR KTCIRNNKVA GNPCPICRDH KLHVDFRNVK LLEQFVCAHT GIIFHAPYTG VCMKQHKKLT QAIQKARECG L LSYYVPQV ...String:
MAAPLRHTLL KLVPTLLRSS YVAQVPLQTL CTRGPPEEDA PSSLPVSPYE SEPWKYLDSE EYHNRYGSRP VWADYRRNHK GGVPPQRTR KTCIRNNKVA GNPCPICRDH KLHVDFRNVK LLEQFVCAHT GIIFHAPYTG VCMKQHKKLT QAIQKARECG L LSYYVPQV EPRDADFGTV HGAVSVTPPA PTLLSGEPWY PWYSWQQPPE RELSRLRRLY QGNLLEESGP PPESMPEMPT TP PAESSIE QPGSQSA

UniProtKB: Small ribosomal subunit protein mS40

+
Macromolecule #16: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 16.300146 KDa
SequenceString:
MAALVALCSG IGRKNLTTAA GCFTDRGTQA VSVIWRRCFS QFEQVTSNED LPVPMENPYK EPLKKCVLCE KRVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQREIT KAIKRAQKMG FMPVTYKDPA YLKDPRVCNI RYRE

UniProtKB: Small ribosomal subunit protein bS18m

+
Macromolecule #17: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 10.493249 KDa
SequenceString:
(AYA)KHLKFIART VMVQEGNVEG AYRTLNRILT TDGLTEVISR RRYYEKPCRR RQRESYETCR RIYNMEMARK INFLMR KNR ADPWLGC

UniProtKB: Small ribosomal subunit protein bS21m

+
Macromolecule #18: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 41.25509 KDa
SequenceString: MAAVRTPLSL WRFQLGSRRA RRVCTRATAQ RHPDALLATR PQPFEVGQPR RLLSSEAESG SSEVKKPAFM DEEVQRILTK ITGLDLQKT FRPAIQPLKP PTYKLMTQAQ LEEATRLAVE AAKVRLKMPP VLEERKPIND VLAEDKILEG TETNKYVFTD I SYNIPHRE ...String:
MAAVRTPLSL WRFQLGSRRA RRVCTRATAQ RHPDALLATR PQPFEVGQPR RLLSSEAESG SSEVKKPAFM DEEVQRILTK ITGLDLQKT FRPAIQPLKP PTYKLMTQAQ LEEATRLAVE AAKVRLKMPP VLEERKPIND VLAEDKILEG TETNKYVFTD I SYNIPHRE RFIVVREPSG TLRKASWEER DRVIQIYFPK EGRRVLPPVI FKDENLKTMY SQDRHADVLN LCVAQFEPDS AE YIKVHHQ TYEDIDRHGK YELLRSTRHF GGMAWYFVNK KKIDGLLIDQ IQRDLVDDAT SLVQLYHMLH PDGQSAQEAK EQA AEGVDL IKVFAKTEAQ RGAYIELALQ TYQEIVTSHS AAS

UniProtKB: Small ribosomal subunit protein mS22

+
Macromolecule #19: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.378051 KDa
SequenceString:
MAGSRLETVG SVFSRTRDLM RAGVLKEKPL WYDIYKAFPP LREPVFRRPR LRYGKAKADI QDIFYQEDQI RAKFFATYGS GQKAFDLFN PNFKSTCQRF VEKYTELQNL GETDEEKLFV ETGKALLAEG IILRRVREAR TVSVRLQASS EGHEPQEDDD L AQRGQVKQ EPETAPSPP

UniProtKB: Small ribosomal subunit protein mS23

+
Macromolecule #20: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 19.95041 KDa
SequenceString:
MPMKGRFPIR RTLQYLGRGD VVFKESVKIM TVNYNTYGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIKKILGKK EETLREEELE KQQRFHPGNF GPRKYCLREC MCEVEGQVPC PGLVPLPKEM T GKYKAALK AST

UniProtKB: Small ribosomal subunit protein mS25

+
Macromolecule #21: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.484803 KDa
SequenceString: MLRALNRLAA RPETRPPTPL LLPVRGRKTR HDPPAKSKVG RVQTPPAVDP AEFFVLTERY RQYRETVRAL RLEFTLEVRR KLHEARAGV LAERKAQQAI TEHRELMAWN RDENRRMQEL RIARLQLEAQ AQEVQKAEAQ AQRAQEEQAW VQLKEQEVLK L QEEAKNFI ...String:
MLRALNRLAA RPETRPPTPL LLPVRGRKTR HDPPAKSKVG RVQTPPAVDP AEFFVLTERY RQYRETVRAL RLEFTLEVRR KLHEARAGV LAERKAQQAI TEHRELMAWN RDENRRMQEL RIARLQLEAQ AQEVQKAEAQ AQRAQEEQAW VQLKEQEVLK L QEEAKNFI TRENLEARIE EALDSPKSYN WAVTKEGQVV RN

UniProtKB: Small ribosomal subunit protein mS26

+
Macromolecule #22: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 47.836418 KDa
SequenceString: MAAPMVRCGM LLARRLDASR LCLAGKRCLL SAAYVDSHQW EAREKEEYHL ADLASLMDKA YERKLPVSSL SISRFVDNIA SREDLDSAE YYLYKFRHSP NCWYLRDWTI HSWIRQCLKY GAQDKALYTL VNKVQYGIFP DNFTFNLLMD YFIKKGNYKD A LSVVFEIM ...String:
MAAPMVRCGM LLARRLDASR LCLAGKRCLL SAAYVDSHQW EAREKEEYHL ADLASLMDKA YERKLPVSSL SISRFVDNIA SREDLDSAE YYLYKFRHSP NCWYLRDWTI HSWIRQCLKY GAQDKALYTL VNKVQYGIFP DNFTFNLLMD YFIKKGNYKD A LSVVFEIM MQEAFDVPST QFLSLYVLYR CLAEKTELTW EEERDFGASL LLSGLKQRNT VGLSSQLYGY ALLGKVELQR GV RAVYHGM PLMWTPGYLD RALQVMERVA SSPEDLKLGR EVLDVLDGVL KVVTSPDVQT SEAQPQEGED GLGSANLVEQ LDT EEPEQS KLPRYLERFQ ASRSKLQELN RVESESLLTL TTQLVKEKLP ACEAEDLATY EQKLREWHLE RVQLIQREQE QREK AKQEY QALSAAEKAA

UniProtKB: Small ribosomal subunit protein mS27

+
Macromolecule #23: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.555336 KDa
SequenceString:
MAALCRSHAG TAGSRFLRAL VFSKPLRNAS TESGSESATH DSSAPRARSG GFASALERHS DLQRKAELRL ESPKPVESFA SMLRHSPLT QLGPAKDKLV IGRIFHIVED DLYIDFGGKF HCVCKRPDVD GEKYQRGTRV RLRLLDLELT SRFLGGTTDT T ILEADAVL LGLQEIRDSK SREEQPSK

UniProtKB: Small ribosomal subunit protein bS1m

+
Macromolecule #24: 28S ribosomal protein S29, mitochondrial

MacromoleculeName: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 44.766293 KDa
SequenceString: MLTGITRLFS RVQKLDPRCF LHMSVQATQN SQVPAERPRT VSRTSDSDPA KHGEQHEGQH YSIPLQDLKT VFPHGLPPRY MMQVKTFGE ACLMVRKPAL ELLGYLKNTN FAHPAVRYLL YGEKGTGKTL SLCHAVHFCA RHDWLILHIP DAHLWVKNCR E LLQSTHNK ...String:
MLTGITRLFS RVQKLDPRCF LHMSVQATQN SQVPAERPRT VSRTSDSDPA KHGEQHEGQH YSIPLQDLKT VFPHGLPPRY MMQVKTFGE ACLMVRKPAL ELLGYLKNTN FAHPAVRYLL YGEKGTGKTL SLCHAVHFCA RHDWLILHIP DAHLWVKNCR E LLQSTHNK QRFDQPLEAS TWLKNFKTTN ERFLSQIKVQ EKYVWNKRES TEKGSPLGEV VEQGLTRVRN ATDAVGVVLK EL KAQSALG LFHLLVAVDG VNALWGRTTL KKEDRTLIAP EELSLVHNLR KMVKNDWHGG AIVLSLSQTG SLFKSRTAYL PHE LLGKEG FNALEPFLPI LIPNYNPKEF ESSFQYYLEN NWLQHEKAST EEGRKELRFL SNCNPEQLER LCASL

UniProtKB: Small ribosomal subunit protein mS29

+
Macromolecule #25: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 43.950836 KDa
SequenceString: MLHRIPAFLR PRPFSGLPLS CGNRDVSVAV LPAAQSGAVR TENNIQRHFC TSRSICSKKV DQSVPANEIS QKAAESQGRG KETLKKDLL DIIKDMKVDL STANVKTPKP RGRKPSASLE ATVDRLQKAP EDPPKKRNEF LSPELVAAAS AVADSLPFDK Q TTKSELLR ...String:
MLHRIPAFLR PRPFSGLPLS CGNRDVSVAV LPAAQSGAVR TENNIQRHFC TSRSICSKKV DQSVPANEIS QKAAESQGRG KETLKKDLL DIIKDMKVDL STANVKTPKP RGRKPSASLE ATVDRLQKAP EDPPKKRNEF LSPELVAAAS AVADSLPFDK Q TTKSELLR QLQQHEEELR AQKDREKRRI SFTHIISNMK IAKSPSGRAS TRPQHQIQFD EDMDSSLKQE KPTDFRKRKY LF KGKRLSI FADKAFADEP PEPEASPSLW EIEFAKQLAS VADQPFENGF EEMIQWTKEG KLWEFPVNNE AGLDDDGSEF HEH IFLDKY LEDFPKQGPI RLFMELVTCG LSKNPYLSVK QKVEHIEWFR NYFNEKRDIL KENNIAFT

UniProtKB: Small ribosomal subunit protein mS31

+
Macromolecule #26: 28S ribosomal protein S33, mitochondrial

MacromoleculeName: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 12.487491 KDa
SequenceString:
MSPLSEYALR MSRLSARIFG EVARSTDSKS MKVVSLFSEQ PLAKKKETYD WYPNHNTYFA LMGNLRFLGL YRDEHQDFKD EQRRLKKLR GKGKPRKGEG KRATKKK

UniProtKB: Small ribosomal subunit protein mS33

+
Macromolecule #27: 28S ribosomal protein S34, mitochondrial

MacromoleculeName: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 25.874777 KDa
SequenceString: MARKKVRPRL IAELARRVRA LREQRNQPRD SQLYALDYET LTRPHSGRRL PVRAWADVRR ESRLLQLLAR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGRAWGILT FKGKSEDTAR EIEQVMYHDW RLVPKHEEEA FTAFTAKPED R LNSVPYPP ...String:
MARKKVRPRL IAELARRVRA LREQRNQPRD SQLYALDYET LTRPHSGRRL PVRAWADVRR ESRLLQLLAR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGRAWGILT FKGKSEDTAR EIEQVMYHDW RLVPKHEEEA FTAFTAKPED R LNSVPYPP LLRAMILAER QKNGDTSVQE PLLNLERTRM RPWDYPAKQE TKGRAKGTPV

UniProtKB: Small ribosomal subunit protein mS34

+
Macromolecule #28: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 36.028289 KDa
SequenceString: MAAAALQLRQ SLCPGPRVLR TFSSVASPAA PRAGPRTASR SERPMRRKAL PPRTEKMDTD QDWPSVYPTA APFKPSAVPL PVRMGYPVK KGVPMAKEGN LELLKIPNFL HLTPVAIKRH CAALKDFCTE WPAALDSDEK CEEHFPVEID TADYVSSGPS I RNPKARAV ...String:
MAAAALQLRQ SLCPGPRVLR TFSSVASPAA PRAGPRTASR SERPMRRKAL PPRTEKMDTD QDWPSVYPTA APFKPSAVPL PVRMGYPVK KGVPMAKEGN LELLKIPNFL HLTPVAIKRH CAALKDFCTE WPAALDSDEK CEEHFPVEID TADYVSSGPS I RNPKARAV TLRVKLSSLN LDNHAKKKLI KLVGERYCKA TDVLTITTDR CPLKRQNYDY AVYLLTVLYH ESWKTEDWEN SK TEEDMDE YVWAKSSSEN SVLQTLLQMR AAESSVAPSR EELLGTKEVE DYQKCVVRLK NEGENEASLA QYKESVKRLL NLA

UniProtKB: Small ribosomal subunit protein mS35

+
Macromolecule #29: Aurora kinase A-interacting protein

MacromoleculeName: Aurora kinase A-interacting protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.345412 KDa
SequenceString: MFLARLTSRL ARTVVPWAGF SRSCPGSGVI GSYAFRPLYS LQPASPSRAA SLPGKRTQSE LEEFLVPRKM AISPLESWLT AQYLLPRRN VEVPVTLAPS QFYECPPRQG EEEAQQGVRE AWDATPVQCK NVLKIRRRKM NHHKYRKLVK RTRFLRRKVR E GRLKKKQI ...String:
MFLARLTSRL ARTVVPWAGF SRSCPGSGVI GSYAFRPLYS LQPASPSRAA SLPGKRTQSE LEEFLVPRKM AISPLESWLT AQYLLPRRN VEVPVTLAPS QFYECPPRQG EEEAQQGVRE AWDATPVQCK NVLKIRRRKM NHHKYRKLVK RTRFLRRKVR E GRLKKKQI KFEKDLKRIW LKAGLKEAPE NWQTPKIYLK NK

UniProtKB: Small ribosomal subunit protein mS38

+
Macromolecule #30: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 77.89 KDa
SequenceString: MAAAAVAARR LSFRSGLVLL QTTRGTGVCE PKVCCRFYAG TESLPKVEGS DITGIEEIVI PKKKTWDKVA VLQALASTVN RDPTAAPYV FHDDPYLIPT SALESRSFLL AKKSGETAAK FIINSYPKYF QKDIAEPHIP CLMPEYFEPQ IEDVSEAALE E RIRLRKVR ...String:
MAAAAVAARR LSFRSGLVLL QTTRGTGVCE PKVCCRFYAG TESLPKVEGS DITGIEEIVI PKKKTWDKVA VLQALASTVN RDPTAAPYV FHDDPYLIPT SALESRSFLL AKKSGETAAK FIINSYPKYF QKDIAEPHIP CLMPEYFEPQ IEDVSEAALE E RIRLRKVR ASVDMFDQLL QAGTTVSLET TNSLLDLLCY YGDQEPPADY PFQQTEHLEN LEEAAEENNQ TSKMESGPWK AQ NNAERIF ALMPEKNARS YCTMIRGMVK HRAYAQALNV YTELLNNRLS ADVYTFNALI EAKTFILNEK FEEKWNDILD LLK HMVAQK VKPNLQTFNT ILKGLRKCYS LGRIPALQIL REMKHIGIEP SLATYHHIIH LFYPRDLSAI KMPSLIIYDI MNEL EGRTF SPQDLDDGRF FQLAMSVCSS LRDLELAYQV HRLLNTGDNR KLVGHDPLRK VYYSKFFSLI CSLEQIDVTL KWYKD LIPS VFLPHYQIFI GLLQALDVAN RLELVPQIWK DSKEYSHTFR DALREEVLML MARDKHPPEL QVAFADCAAD IKSTYE DQS ARQPAFDWPA NPLQYIAVLF LRGGRSQEAW KMLELFKKHK KIPRNELLEE FMDTAKASGS TALAIEVVKL ASAFSLP IG ESLAQRVVMD FTVDPEQKEA LGNLTELNSS DGESSSDSDS DDK

UniProtKB: Small ribosomal subunit protein mS39

+
Macromolecule #31: Putative ribosome-binding factor A, mitochondrial

MacromoleculeName: Putative ribosome-binding factor A, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 39.38227 KDa
SequenceString: MWAGVAGLRG SCAGLQALWG GHRAALLLGR SPALHTSVAS CGSKNLLKKF ASKTRKKFWY EGPSLGSHLT PRPSKHEFLT KTTSKKTRK EDSIRLRVLN GLLYKSLTEL LCTPQVSQEV YDLNVELSKV SVTSDFSACR VYWKTGVSAE QNKHTESVLQ R SAAYMRHL ...String:
MWAGVAGLRG SCAGLQALWG GHRAALLLGR SPALHTSVAS CGSKNLLKKF ASKTRKKFWY EGPSLGSHLT PRPSKHEFLT KTTSKKTRK EDSIRLRVLN GLLYKSLTEL LCTPQVSQEV YDLNVELSKV SVTSDFSACR VYWKTGVSAE QNKHTESVLQ R SAAYMRHL LISQQTLRNV PPIVFVQDKR DLVLAEVDRL LAEADFGPPD ERDDLDGLRN ADAQVPHDSP EPAAHPNLCG ID HEALNKQ IMEYKRKKER GLQCQSLAPP SGREQAPEPT RLLRKREKVR SRWQHRDASP RSFLLGEEDE DEDSSTEWEC HAH EAEDDD DHQEEELGAD RGAQRGLCGK REQG

UniProtKB: Putative ribosome-binding factor A, mitochondrial

+
Macromolecule #32: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 42 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #33: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 33 / Number of copies: 7 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #34: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #35: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 35 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

+
Macromolecule #36: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 36 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

+
Macromolecule #37: water

MacromoleculeName: water / type: ligand / ID: 37 / Number of copies: 1017 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
0.2 MKClpotassium chloride
5.0 mMMg(CH3COO)2magnesium acetate
25.0 mMHEPES-KOH buffer pH 7.5
0.05 % (w/v)C24H46O11n-dodecyl-beta-D-maltopyranoside
0.25 mMC10H17N6O13P35'-guanylyl imidodiphosphate
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 297 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.6 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 165000
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-20 / Number real images: 26468 / Average exposure time: 4.0 sec. / Average electron dose: 31.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 3076615
Startup modelType of model: EMDB MAP
EMDB ID:

10021

Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 3.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 3.06 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 26468
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial modelPDB ID:

6rw4


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL
Output model

PDB-7pnu:
Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA inward conformation

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