EMDB-13477: Small subunit of the Chlamydomonas reinhardtii mitoribosome

Basic information

Entry

Database: EMDB / ID: EMD-13477

• Title: Small subunit of the Chlamydomonas reinhardtii mitoribosome

Sample

• Complex: Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome
  • Protein or peptide: x 38 types
  • RNA: x 4 types

• Keywords: Mitochondria / mitoribosome / alga / RIBOSOME

Function / homology

Function:

mitochondrial small ribosomal subunit / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / small ribosomal subunit / rRNA binding / ribosome / mitochondrial matrix / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / nucleus / cytoplasm

Domain/homology:

Mitochondrial glycoprotein / Mitochondrial glycoprotein superfamily / Mitochondrial glycoprotein / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein S19, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S3, C-terminal domain / Ribosomal protein S19 / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S14 / Ribosomal protein S2 / Ribosomal protein S13/S18 / Ribosomal protein S19 signature. / Ribosomal protein S14p/S29e / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S13-like, H2TH / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / Ribosomal protein S11 / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / Ribosomal protein S13 family profile. / Ribosomal protein S10p/S20e / Ribosomal protein S9 / Ribosomal protein S11 / Ribosomal protein S17/S11 / Ribosomal_S15 / Ribosomal protein S15 / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal protein S12/S23 / Ribosomal protein S15 / Ribosomal protein S11 superfamily / Ribosomal protein S9 signature. / Ribosomal protein S12 signature. / S15/NS1, RNA-binding / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase

Component:

Ribosomal protein S10 domain-containing protein / Uncharacterized protein / Mitochondrial ribosomal protein S15 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S7 domain-containing protein / Ribosomal protein S3 C-terminal domain-containing protein / Mitochondrial ribosomal protein S16 / Mitochondrial glycoprotein / Mitochondrial ribosomal protein S18 / S5 DRBM domain-containing protein / Mitochondrial ribosomal protein S19 / Mitochondrial ribosomal protein S17 / Uncharacterized protein / Mitochondrial ribosomal protein S11 / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / 30S ribosomal protein S9, chloroplastic / Mitochondrial ribosomal protein S12 / S1 motif domain-containing protein / Uncharacterized protein / Uncharacterized protein / : / Uncharacterized protein / : / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein

• Biological species: Chlamydomonas reinhardtii (plant) / Chlamydomonas smithii (plant)
• Method: single particle reconstruction / cryo EM / Resolution: 4.2 Å
• Authors: Waltz F / Soufari H / Hashem Y

Funding support

France, 4 items

Organization	Grant number	Country
European Research Council (ERC)	TransTryp ID:759120	France
Agence Nationale de la Recherche (ANR)	ANR-11-LABX-0057_MITOCROSS	France
Agence Nationale de la Recherche (ANR)	MITRA, ANR-16-CE11-0024-02	France
Agence Nationale de la Recherche (ANR)	DAMIA, ANR-20-CE11-0021	France

• Citation: Journal: To Be Published; Title: The Chlamydomonas mitochondrial ribosome: how to build a ribosome from RNA fragments; Authors: Waltz F / Salinas-Giege T / Englmeier R / Meichel H / Soufari H / Kuhn L / Pfeffer S / Foerster F / Engel BD / Giege P / Drouard L / Hashem Y

History

Deposition	Aug 26, 2021	-
Header (metadata) release	Jun 14, 2023	-
Map release	Jun 14, 2023	-
Update	Jun 14, 2023	-
Current status	Jun 14, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_13477.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.13 Å

Density

Contour Level	By AUTHOR: 0.015
Minimum - Maximum	-0.04388904 - 0.08191181
Average (Standard dev.)	0.00025500625 (±0.003984827)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 406.8 Å α=β=γ: 90.0 °

Supplemental data

Half map: #1

• File: emd_13477_half_map_1.map

Half map: #2

• File: emd_13477_half_map_2.map

Sample components

Entire : Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome

• Entire: Name: Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome

Components

• Complex: Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome
  • Protein or peptide: mS35
  • Protein or peptide: mS45
  • Protein or peptide: mS45-insert
  • Protein or peptide: mS31/46
  • Protein or peptide: mS105
  • Protein or peptide: mS106
  • Protein or peptide: mS107
  • Protein or peptide: Unk1
  • Protein or peptide: Unk2
  • Protein or peptide: Unk3
  • Protein or peptide: Unk4
  • Protein or peptide: Unk5
  • Protein or peptide: Unk6
  • Protein or peptide: uS4m
  • Protein or peptide: Mitochondrial ribosomal protein S6
  • Protein or peptide: bS16m
  • Protein or peptide: bS18m
  • Protein or peptide: mS26
  • RNA: S1 rRNA
  • RNA: S4 rRNA
  • RNA: S2 rRNA
  • RNA: S3 rRNA
  • Protein or peptide: bS1m
  • Protein or peptide: uS2m
  • Protein or peptide: S5 DRBM domain-containing protein
  • Protein or peptide: uS15m
  • Protein or peptide: mS34
  • Protein or peptide: mS23
  • Protein or peptide: uS17m
  • Protein or peptide: uS12m
  • Protein or peptide: uS8m
  • Protein or peptide: uS11m
  • Protein or peptide: Ribosomal_S10 domain-containing protein
  • Protein or peptide: Mitochondrial ribosomal protein S13
  • Protein or peptide: Mitochondrial ribosomal protein S14
  • Protein or peptide: Plastid-specific ribosomal protein 4
  • Protein or peptide: uS19m
  • Protein or peptide: uS7m,Ribosomal_S7 domain-containing protein,Ribosomal_S7 domain-containing protein,Ribosomal_S7 domain-containing protein
  • Protein or peptide: 30S ribosomal protein S9, chloroplasticRibosome
  • Protein or peptide: mS29
  • Protein or peptide: Ribosomal_S3_C domain-containing protein
  • Protein or peptide: mS33

Supramolecule #1: Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome

• Supramolecule: Name: Small subunit of the Chlamydomonas reinhardtii mitochondrial ribosome; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
• Source (natural): Organism: Chlamydomonas reinhardtii (plant) / Organelle: Mitochondrion

Macromolecule #1: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 88.892773 KDa

Sequence

String:

MTLSTQRRAL LAGFGKARGG QWTQEGIAAL HSTTTTNAQV ETQPDGELAE SSADDASRLF QELSRRNRPN SAGTEPGPSP RPVLAQLPA AAELMERAAG TPSSQALYDL PTYLSVTHPH ARVEPRNPAY DWRRSPQLEA GGPRRAALLL AVDAHMAAPE S REALLRMA QLELAHLWYY QQHAAELPAA ASPSASAASA ASTATPDAAA AGQRRGGVAK QREAEPAAAS TSAAAGDKAQ AG AGTGTGA GAGAEAEAGD DDIFAAADRK ARERAAAVEA ATAAAASSAA KSLGRRGGRL PAELEARVRD MQLRYGMASR DAA SVLRLL ADSFSRRLPG GGRRLEGEAE AEAAVGLGEA EAEAGGDPTA ALTWALSGGG RGGSGGTISG LSRHLAKQAA QAKA ADRAI ITAMQSLADA VNTASSSSSS ATASSSTASS SGSSAYWAAH PALAYDQTLA ARAHRQGLAW RAAAEAGPDG AASLS AAIT ALSASLRRPT SSPTSSASAS SPPATPVLDL VLDYLQAKYA DLLTAWETAA RLREATERVS AAVARARATV PMSAVP PLP PALAAELERS WQNAAAAFRP ALLQPLLQPA GSGAAARNSL AAAAASGSPA LLQAQSSLTR PLDWAKAKAL IEQHYAK QQ QAAALAGATA AAEATAAAAA AASAAAAAGS PRGAVEALLQ PLLQRAADRH RALLAGGDAA AAEAAAAAAA AAAHGSST A AAGGAAPSEG AASVVCFRET LTLDASSAYA NSSFDSRVTL EFNVDRLAAA EPGLGGAWGA RFLERLLATP ALPGSGRRI RGGGGSSGMF GRARASPAAR AAANAAAYPV DVEHSFCRRT RTAALSTARY GSREANRKLL LEHYNELLRA AALAAAAGAS AAV

Macromolecule #2: mS45

• Macromolecule: Name: mS45 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 102.234461 KDa

Sequence

String:

MRARLFQSLA IQVAEEGLLT TALSGYACRA FSANGVTKFG FQCSSVSTSA ASHEASSSRG SGDQGRVTGG RQERAGRQAP TGSDGPQRA DRAKQGYADG GKSRSLFRRD NNPDSGSRGG DGQRGGGKGA GTGSGYSGGS SGQRGGPWQQ QQGGRQHDRR G DDRRGGGG GGGPPPMLRD GRDGRDGRGG GGRPPFAPSG PGSGSRAPRA ARWDALDVAA SNAPQRLLQL VGDPRSGVAD DT SSPAFGE VTVELLHHQP SNEVYWYKYA FAGGQPGGRG GHRDGRLSDY SRNLAYVLRA KDPARWSVEA LADKFRVRPQ RML AALALK ELEAQRLESG ALLRGPLSAY SLRVSLADVH LDKASGAPRL RPASAPPAAS RELLLGRMLP RVVSATQRTQ AALL SALRP HGFDAAALRA ALQADVAAVE AAAGELDAAA AAAGGEGAEG GTAAGGVSAY LAPELAACRA AVEAVAAAVE VVVNA SPLL QEERQAAALR ARVEAALQAG AGQAPAAAAA VAGTGAGAGG GADGNSKDAV PAAAAAAATS PGGGEALEVL LQPLTQ DGS GAPLQRFLFS LPPPIRVALL RQLPRLAAQL AAAGVDLQRA GRDAAYRGPL APAAPAATPA GSKQQQQQRQ KQGGAAA ST RSGKLPRLTV DVLGFVPVSE AQAEALAAAV ESAAAAEAAL VAKLEALQVA AGAAPAPAAG AAAAAAAAAA TAPSAAEL R DARSLLRQYL STHSMWGELR LAARKAAGHS PDTRGKRRKL EVMAEADPAA LTRALQVEAH TQLRRSHDRA AAESLKAGV AAAAATEAAA AAAASGSEAA AKPPTPLSDP AGWDALAAWL DAEVAGRVYA RGSGERLVVR LPTYPAFEGY PLSELDKARE GELGALNRL VAARTAAAQW AAFRRDLLFN LGVRGEKLRD NDLPGAPAWP ANLRTELTRP VVVYGIGPDG ATQYPPVYVA A GDGAKRPL NEQEKVYQER RLPGPRLPYH MGRIRTLPEL N

Macromolecule #3: mS45-insert

• Macromolecule: Name: mS45-insert / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 36.187551 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #4: mS31/46

• Macromolecule: Name: mS31/46 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 15.847501 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #5: mS105

• Macromolecule: Name: mS105 / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 28.879045 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)M ATRQRLMRGI AGLLHQQGQQ SSCSQLLMTS AAAASASASS VATGPSHQPA LLLRAGSALR GVTTSAPTF NGSLSSLLRE EVDYERKNYE RPAQISGGPP APFKLTEAPG DTLLTLTRTF GAEEINVDLH VNSQPSPEYD GEDGDEGISV VAFNVSVAK GDRVLLFECE SDGNSVNINH VSLEPKEGLG SESMYSGPVF DELDDNLQGQ FGKYLEDRGI TAELGEYLRF L IYDKEQRE YQNWLSEVEA FVGGK

Macromolecule #6: mS106

• Macromolecule: Name: mS106 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 51.927285 KDa

Sequence

String:

MDLIGRGGAP SASALGTDAL VLLCGPEAQA GISTCSAAAE SAASPQSSCS WVGTAQHSSA TRAEAAGPSA PCGPAPLLRN LRTPSQSGR LSGAPPTWIS AAAASLGNSP RFAASARCST ADVDVSRSGL EDASALGDCV DRWSTPAATV SGIALLHQYH N HHHNHHHI GHSSSSCGSV ASSTSSVPGS AAGSPFPPRP SLPSSATNSS RFLMQSQSQQ LRSVSFTAAT AAPKAAAKGA SA KAGSSSS SGAAAPSPAA AALRTPEWAR VPGHLHELQD LYRKRQKRMA ALRHGAQVEL EAREAVAWKT GGRGRAVAAA AKA ALDALP LPPPEDGGKA ELAAALAADT TFAAAHEALT GLLRRGVVLD AAEHLAPLLR KAGDAGQLAS ALGVAAANHL SQAA RQLGA HSPRHGPLHA VLVQECRRLK AAAPLVTLWE SLHEHGLAPE AADAAAAVRA AVELGDGGAA VRLLMLACMY GEAPL AGAA EAGAVLQLLQ QGNPDQAAQL RELLPKLGLR GA

Macromolecule #7: mS107

• Macromolecule: Name: mS107 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 43.833246 KDa

Sequence

String:

MRKRELLNEA RALVPEGSGW LEAYTRNISP RQLTWRLGKR DSLAAMTEGW QLYQGKFDTV AMAALLRRLR HAQLQDPGFD PLAAQRLLD DLVPRLRSVG LRFGKLRDIT AYLHALAKLR SPAPSASSSA ASPRAGAAAA SLLTQPDALV LDLAVFATRN R TELLHASP QRLATLLWAL MRLLPPQLYG SEQLQVVLDR MALASLGRLQ NFAPLDLRWA ALAFATFGPH GPSSKATATT TA AAAGTRA AAGAGSGPAL PEWPRVVRGE AAAAATAGAQ GAEDVTARRQ SRNARVIKAL CDELAARSGN LALPQPEPRD LAL AAHALG LVAAASSSAG GAVAPPALLV KAAGVAARSL PALSGEEAVG LVETLAVWGL RQPALLEALR DAAGRWGEGE QAEA LRGRL QAAYTRLGVE L

Macromolecule #8: Unk1

• Macromolecule: Name: Unk1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 2.826475 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Macromolecule #9: Unk2

• Macromolecule: Name: Unk2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 12.95396 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #10: Unk3

• Macromolecule: Name: Unk3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 4.613678 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #11: Unk4

• Macromolecule: Name: Unk4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 6.911511 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #12: Unk5

• Macromolecule: Name: Unk5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 9.209344 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #13: Unk6

• Macromolecule: Name: Unk6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 48.613012 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #14: uS4m

• Macromolecule: Name: uS4m / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 43.497859 KDa

Sequence

String:

MQRTLRSLAT RCAGAITGST AQTGASGCIK AEAGVSTSAL SGLISQDAIH GLCPRGAAFA SLASSGVSAA AGAGPASQLR AGAPLSCLW LLALAPSRTG LAATASSSSS CGACGSHSST SSPFAPLPAS AAGLRHYAKA AKGGAAAAPA APSGPKRPRT S TTKLYSCR NDRLRHTHEQ IWPTLQLTEY EQAMFKRNSR LFVVDMGRSL SLRDKFRMGA YEPATAASTG AAAEEAGGAG AG DALVPAA GGASYRRVPY WQARSLLHES NLHLDALGEN PRYLRLRRVG SLFATKLQNV RKLRLLLGFQ RRGFVQKLYE HSL LARGSD RMWKMVCAME ATLPMTVTRM GLAEDVVGAA TAIRNDKIYV NGKQPVMPRK GLLEPGDVVG PAAGGAAYLR KRVA RSMEP LASVVTRDYV

Macromolecule #15: Mitochondrial ribosomal protein S6

• Macromolecule: Name: Mitochondrial ribosomal protein S6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 13.661864 KDa

Sequence

String:

MPLYELLMLA KPSLPRAEMV ALIKGLGDLV YRNGGFVTSL KSFGDQHLAY DLRRPFEKYD RAHIWQMDFV SSVDAIKPLD HGLHVNNTV LRWVLVKRPM SDKSAAAPAA ATQAVEAIAQ PQR

Macromolecule #16: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 10.058308 KDa

Sequence

String:

MEKRQARNSG VRKEDVGWWD PNPAADGNMH LGLNFDRLKY WLTAGAKPTD KVAELLGHAG VLPKVPQPPH YNPRDPKDDT KWRPNEDK

Macromolecule #17: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 36.901188 KDa

Sequence

String:

MPACSGLHAH ADQQSSLLQL LAQRKGAYSG RPDINQQSRG FASSAGSDNS SSGQQAAGLE SERLAPTTRL QDLVAGATER IAQKQVGTL GQLPEQLAAE AASLEHQLAS SSGRSGHTHD LDGAGALADR LASAAAAGGP DGRAGGADGA AVTAGQRLLH N KYGYAALG GVVPDGGEMM AAAGPAAVLR SVTAAQPRLH PRRRFQPGQT YEPQDLNPYS SASPESRQRG VGVAVPRPSV AE VLEKADY KNVAFLTRWF LSPAGRLLPR RQTRLPVAVH KHVSRQVRLA RHMGLIAGEA RLDKTHVQAL REVEAAQLLA GRL VAGAEA GAQQQAAAGA GAGAGAAPRQ RYNELLDFGA

Macromolecule #18: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 22.474727 KDa

Sequence

String:

MQSGTVQKLL PSAEMLSALL PGLFVAQTRS KTYTSNVMDE TRTKRIIDHF AYHKARKQYR ASLHNLYSSW RAELVAKQLA DPGAQRRLK AEEAREEAAR MAELLREKRV ATLVEQVRKA ELEHKLAQVQ LQRAVRRQEE LHRREEARAA RYQRLLEASR N WIRQEDLE AAINRALDNP EPFGFVTSLK INRGF

Macromolecule #23: bS1m

• Macromolecule: Name: bS1m / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 26.942551 KDa

Sequence

String:

MRARRSPAIN TLDAQLQQAD ASPASAPSPS SPSSAPLAPS DLDDVLCRHT LRLPKPASTG QEAALRLLSR FNGALPSLRG QTILAKVLS VDSERVTVDT GYNGVAEVPR ADVSIAHVHT ADGAAPVRPT TSDVRPGDLL RLRVDAPYTP YGDMQLTAVR E ESEFKRRL AWGELRRAME AGQPVSGRVL NDCVGGFAVG VAGFVAFLPS AMAARDTTQR VGSLQSFRIV AMDEARRRIT LR DAGVAAA GFRRVG

Macromolecule #24: uS2m

• Macromolecule: Name: uS2m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 53.354316 KDa

Sequence

String:

MLTRLAALGR PAVLDAAAAA VVVAVEPSAS ASAAWRDGGR DASTSYGVHS LPLAWGAQRR ALGALASGCA STAPTPMVAS AASAAAQAR TGLPFSTAAS TPAPVNAPPP PVNAPHLSNT PAELEEAELA GLDMESLMRA IVHPLPLGRV RNGALLAQAL G TGAPVVQL ENGPGGRDAA LFFDPAATLS STMRALHVIR QVLEKDGHVV VVNSNPRMRP LLREAAHLCL NSNVWFWADD WL PGCLAET DARGGHCPLL SDKAQPNRQI MAEKGLVFRN PLVPDRGNPA AVLGGPAPRL SAADMQRLLA GARSPMGLTH SVY KREQTA SHRGCRKALA ALTAARTAEA RLLLPGSQTG AGRGRFRQPA LVVVLDLSYG GEAVREAAAR NVPTVALLNG HSDA SAVTF PVYASESHLG YHHFFLEWLL RVVNIDPKVL HTLRNAARAA PSAGATGATG ATGATGATGA TGATGATGAT GATGA TGAT GATGATGATG ARAAHAGAGA GAAAAPAAGS GKAAGKAGQQ GGRGKR

Macromolecule #25: S5 DRBM domain-containing protein

• Macromolecule: Name: S5 DRBM domain-containing protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 38.876633 KDa

Sequence

String:

MLRGSILSAQ STGAFSCGAA AVMPSLRSAN SLHGSFAASA AFSSPISTTT SCLDVSQNQE RGLSTEATGE GESEDDPTYL RGIKTQSGL RNSDGDEEPP DRRKAYEFEP GGRGRGGGGG GGGAPFQRRP ADAAAAGTDM LELVATAREL FTRNVDSGSG G GGAGGGGG GGGGVGPLPP LPRELREALY GHRWDRVIVD VRRTHTPVRG RGKREEYTAM VATGNMRGVF GLGIGVAESA QL ATARAHL DSLSRLAAVP LYRGHTLYTH VDHTFHRLSI RMMPRPAGWG LRCPDLLYEL CGLVGLRDVS IRVRGQRGSR FFV AQCFQE ALQKQTTPHD GVEATGMYIR EVFRGGPGGR LPCGLRRGVD VM

Macromolecule #26: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 34.706652 KDa

Sequence

String:

MAGRLLSRLG LIELGASAAC AARQPWPATT TAALPMAVLS ALHPWRLAWV TPCGALLLST SSSSGGAAGA TEAVSTSAPF VEPSPSTAA GASAGSSSAD IAGPSGDSKP DLVEKLLSSD VLGKGERRRY ERQALIREFQ RHPADTGSPQ VMVAVWTHRV R ELVKHFDA NRKELRPMRE LEIMMNQRRR MLTWLRRADF ESYAYVISKL GLQDIYTSVG ISDRYREGLR PSDPV(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)KDDS VNRLRFNFHK QYKQKKTNLW QRLRPQLLAE DPVLAAQAVA TAAAEKQRQT QQRNATSQRL PAQ

Macromolecule #27: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.287194 KDa

Sequence

String:

MAFVELLRSA PKRTLSDLLR KLPNHGVGAI VTRDTWHPES KKYWEVVEVA PSTADPTKLS AWGYQYFKGE RQHPAPKRIA SVWKYGWLL KEQPGAGLSA AVLQQLSERQ AAAQAAGLVA GGSEAAAGTG AAST

Macromolecule #28: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 20.813639 KDa

Sequence

String:

MEFWRLVVRP TRRANLVAKL MRDLESGHFA APKALDVLTQ YRTEQLSYSL TGIPRVTLPE KPLIRAFLQK YPEARAEPVA LDSFTPPLA RQFAQRQLQL MQAGAAREQA FTQAEQELAG RLQALRSRLL GSAATALSEG AQAAVPGPAA SGVRGMVELL Q QEEQEALD AGLEALASSA QQQQQQLSAN SR

Macromolecule #29: uS17m

• Macromolecule: Name: uS17m / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 22.409297 KDa

Sequence

String:

MGGMEFIGRV VSNRMQKTVV VAVSYVVWVP KYKVYQKRVA RHKARDESQA SVIGDIVRIR KSRKFSREVS YSVVDTLRKA HVYDPAAAA ALVATRDAAR AAAAASTGTR AGAAAAGEGQ AAQLEGAGFA ASGVATSSAG GRSDPWVAEA ARRLDASAAR L AALRELYE KEVGPGVPLS GAVLAVANSG PGGTGSVRGK AAAAAAGAAE GPGDAKAQQD KQ

Macromolecule #30: uS12m

• Macromolecule: Name: uS12m / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.082508 KDa

Sequence

String:

MRVWSEVQAA HMATVGQLLG GARKVAKTRK CRIRQLDGSP FKKGICLRVY TTSPKKPNSA NRKLAKVQLS NGLKALAYIP GEGHNLQEH SMVLVSGNGV RDLPGVKLRI VRGRYDCAGV KDRKKSSED

Macromolecule #31: uS8m

• Macromolecule: Name: uS8m / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 44.616223 KDa

Sequence

String:

MAAPLLDPLV SKLRQTTATA ARAAEVMRAA FPGATHETAG RNTIAVQLPR KDVPTYVMAN QRPQPWELLP MKAAAMTQYP NFFNNSCTF FGSIKRDVVN GVPFCLLRPS RLALDMAKVV RNLGIVDGFE VVQRRSRLGA HDFVWLPEQQ PQEPEHLYDT S LFRQRLIR LHLRTDLFSR LPGAPGSGAG GPQPASAQLA PAVGLLPLSV KNISKASQPV LMYPRQLEEA AARLPAGVFM CY HPQLGLI TDAMAQQYDV PALVAAHVGL PLSQAAAIRG ALRVKAAEEA GKELRHVTQL KDWNMMELLR QRMVERRAAL EAG MGVGGE VAARLQELRE AGLRLRDEAS DRVTSALNVA QDLEDGALAW QLVHSRALGA APGAAAAGVD EGAGGEEQAG AGEG RTSPR GQPRRRR

Macromolecule #32: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 23.121113 KDa

Sequence

String:

MAQASKGLIG ALQASLGGLL STSGRLHGGL GPSAGPAALV SGAWGLSACA AFTRSVATTS AGASATAPAE PGPASTASDA GSGAAQEAE AISSGSNSGG GGFSTGFRKV VSERRAGQLG ALEGIVHIQN TLNNIILTLT DKQGLIKTYT SAGVVGYKGS R KSQPVAAE KAAEELARRA LKLGYSSVQV RLKGAGSNKQ YAVTSLAAAG LTITSLADVT PVPYNGCRLP KKRRV

Macromolecule #33: Ribosomal_S10 domain-containing protein

• Macromolecule: Name: Ribosomal_S10 domain-containing protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 61.617887 KDa

Sequence

String:

MQPVVLRGIS SLTWQHARGA LPSALQQALA ASAASSSSSL TAVDCDAGAD MQASCSGRGV ECARHRLAWG PLSGVAGLST SARAASPAA AAMPSSSAAS AASPAADAAP PGAYSVEIAV QGFEKRYVDM ACNTLGDIII LAFAPKSYAA LPTGAPSPSD A PVNLAFGP ERRDVRLPWR RTRITLIRGP HIDKTGMEQF ERREYKSVLT AATNCPQELA RLLEAVKLYQ FTGVQIKMQL AS AQRVDLP ADVSALLRGG GGATAASTGS SSSSSSSSSS SSSLVGSPVV (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)EIPAPA SADLAFAEEV GRVLGVLRPA VWAG LSRRR AVLAGSADHE TWLKAAAGGL PPSASATRGA GRGGGAAALP PPAVVAGWDA EAEVAGAAAE GGDAFGRLLR LRLRQ QAQL QDAQASTSAS GTSTPAAASA AAYGLDPALA ARLLARIDAD LLHAHEAAAA GLPPPAALAA GTAAAAVAAP PPATAV LDA SKMSPAEYAY AVLKYVQYVD SLYESSGAAA AAARGDAALQ LAAPALAVRL LQLWWEATTV EFKRALGLPP AEEERRM LE RLLELQKKKD ELKGAGRGGG R

Macromolecule #34: Mitochondrial ribosomal protein S13

• Macromolecule: Name: Mitochondrial ribosomal protein S13 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 13.758888 KDa

Sequence

String:

MAQIQRVALF PHATFHIALA RVFGLGKSTS LAVSEACGIS KDLKVKDVKD SYIQKVTAYI EANFVTGDTL KRQVRENVLE EVAINSRRG VRHTLGLPVS SAQTRSNGVN ARKLRPLLLY DTSRR

Macromolecule #35: Mitochondrial ribosomal protein S14

• Macromolecule: Name: Mitochondrial ribosomal protein S14 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 13.009181 KDa

Sequence

String:

MKFRLPQYAY IIGTRGNVKD HMRRMLVEQH EVDRSVFKAV TTDKSVPLEH RLQVQRLFET EVPRDSAANR VVNRCVLTGR ARGVHRFAR LSRIMIRQLA HSGFLPGVSK ASW

Macromolecule #36: Plastid-specific ribosomal protein 4

• Macromolecule: Name: Plastid-specific ribosomal protein 4 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 14.437036 KDa

Sequence

String:

MNQSTSSLVR LASASFAAIG ECTSNLARQL FLSSTLTQFR YTTDSVHQPV NEVQSSSTGP ASTICGKGDN RTRRGKIFRG TYREQLPSH GPQLHPYPGG PVVQRPGGGS RGAFRYPGEA PPTAPPGASH TVPPAQR

Macromolecule #37: uS19m

• Macromolecule: Name: uS19m / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.951077 KDa

Sequence

String:

MPRSTWKGPY VAVSLLQEVV ALARKHPNWW NKGRYIGQKA PEVINTYSRA SVILPDFIAC RFGVHNGKSF VGMEVQEAMV GHRLGEFAP TKATVQHKAK EVNTQKKKIN PKTGKAA

Macromolecule #38: uS7m,Ribosomal_S7 domain-containing protein,Ribosomal_S7 domain-c...

• Macromolecule: Name: uS7m,Ribosomal_S7 domain-containing protein,Ribosomal_S7 domain-containing protein,Ribosomal_S7 domain-containing protein; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 32.755266 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)MLSSA RRLLATVAEQ RALAKADAPA AAAQLRARA GLASVSCCGS TTATITTRLG ATGSWRHMSS AADSTSSSSS SSSSQQQQPP AQQQQQPEAS TSSAAASSPD G PMSEVVQQ LTKASPTGRL LDLFTPSQQQ IQTLQRSRGP GRGSRSGTGP AGPAAGTGVQ AAEAAAPLTA DSDVLEVLMR AV DNCKPLM KVIQSKAGTR VVYQPRPLNP DQSTNFAVKW ILQAAQKRRA GAKGVAGAAS MAEALAVELL LAAQRKGGAR ARR DEVHKL ALDNRANLRR

Macromolecule #39: 30S ribosomal protein S9, chloroplastic

• Macromolecule: Name: 30S ribosomal protein S9, chloroplastic / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 45.610211 KDa

Sequence

String:

MVRGGVATVG PSSLALAASA LLGTLPQQTA SHSGASLWVF GSRHLAAPAA AGGASNQDRT QPSAKPSAAE PELKLNFSLR PSVLSPLFP AWEQTRQINQ RREHLMALLD AHQQLAEGLR ITKDGVAAST DGADAGTTSS FAAGGVRAGV TRSARRRARN L QQVASQLR ALLDATPGHP RGVTGLPTAC PSPAHAVAYC GALEAALASP DAASTGARDF GAVARGDVSP WTHYAKHFAG RY AGAPQLQ PLEVIYSRPD PAEVVRQLRR AAREQEALGI SSSGGGSGEA GSGLAFPATS TSPEPAGPHI DLTGVTHARG KRK ASAASV QLVPAGGAGI TVNGVPLSSY FRDPACREKV LAPLALAGAE TAYDVAARVK GGGMMGQAQA IATALARALC VQQP SLGAT AGDGGNGEGP LAAMTRWDGR VVERQKPGQA GSRRGFQWVK R

Macromolecule #40: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 49.635844 KDa

Sequence

String:

MTSALLVASR RARQAQGLPR CLLHAIGIHA GTRAEFASVA LQEAGTTPST SGQEQPSSAQ LTPAHLRSYY PLNLALLPEA ARGSAGAFY TPRDPGHERR GGCKALQQEM EATGRASILY RPIMAALNGA VAAGQQPRLL LTGPAGCGKS LALLGLVEWA R QQGWLVVY VPSCLALVRG GYFARRGRGA AGGWDTLTSA QQLLKGVMDA HGPLLQSLPV LPVPGRAARR QQQQQHEPRQ AD KPAKVEE GQGQGQGQAG GSGLLEEGSG SASGAGGGGG RTLQDVALRG LSSDDNAQLA VDSALQLIRQ LQLLGSGAAQ PPD SQPGQP PRVLFALDDY NYLYGPTDYG VQPPSASPLQ GRRRVLDAGE LILARGLRLL ESELGTNPVA AAAAGGGAGG VGGA VVVAA TTATPALPAP RSLALEVPHT VVEVPGFDEA ETAAALAHYA ATGAATRAAS AAEARHLFAL TGGNGRELRA KAGAL GVRV G

Macromolecule #41: Ribosomal_S3_C domain-containing protein

• Macromolecule: Name: Ribosomal_S3_C domain-containing protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 50.704199 KDa

Sequence

String:

MDDMVLVLAR NLSGKAKGKP APKGAPAPPP PPVASSAVTA NPATRSAGAR IRRNQPRLDP LTLLTPQQRE LAESLAHLSR QAAQPPSAT AAGADALTPA AEAAADAAAE AAAAPLRGLG EVPLPLRRRE VAALVDAALT GAAGGQQVHF QPFVFNDIFQ S APAAATYV AEALESGSAW GRIERFFLAA AEADAGKLVA AVRVEVAGRI GRKADMAATK SWAWGDLRLA SLTSKLDYGT AA ANTRMGV LGIKVWIRYQ PAAAPDLYFP ATGGSGLHAP LPAGAAPSMS LDELYAAALE RSGSSSSSSS SSSVGGAGGS STR YGAVGW WSRPAGQQPA ANRVHEVFTP EFNPKAGDRL DGYLRRAAES RRVFRSAANR EEYMATVGRF LVKQRQAAKA RAAA EAYAA ALSATKPATI PTTSSSSSSS SANSSAASSV PARPRLGRRP HRPLARFPVE AFSPLGRRGP VLLRWRLQAA AAVGK GKGG NGERRA

Macromolecule #42: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 22.289773 KDa

Sequence

String:

MSASTLSRLL PAGSLAWGLP GVLLRSASLL VHHQPCAPST SGRVQESQLQ WSCYYATSHG TGAPKAASTQ SSSTTSAASA AASATSAAR PAAVSSEAGG DGASSAPFYA GLAARYPDPD TVPDHVMHRL RNTLFGRAVR PRETTGRRAL ARPLQGKALT D WYWMPPNE SPGFHSEEDE YELRRALNRR HTKEAEAEAA DTGADKKKKR K

Macromolecule #19: S1 rRNA

• Macromolecule: Name: S1 rRNA / type: rna / ID: 19 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 32.590293 KDa

Sequence

String:

AAAUUAGAGU UUGGUGCUGG CUCAGCUUUC AUGCGCCGAG CGGUGGUUUA UACACAUGCG AGCUUAAAAG AUUAAGGCCA CGAGUGCGU AAUACGCGAG UU

Macromolecule #20: S4 rRNA

• Macromolecule: Name: S4 rRNA / type: rna / ID: 20 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 133.231078 KDa

Sequence

String:

AGCUCUUGCA UUGCUGAAUU UUUUUUUUUU UUUUUUUUUU AAAAAAAAAA AAAAUUUUUU UUUUUCAAGU CAUCAUGGGG CUUAUAGAG UGGGCUACAG GCGUAUUACA UUGGACACCC ACAAGUUGCC AAAACUGUCC GAAUAUACGG AUUGGAGUAA A AAAAAAAA AUUUUUUAAA AUUUUUUUUU UUUUUUGCUG AAACUAGCCU CCAUGAAGAA GGAAUCGCGA GUAAUCGUAG AU CAUUAGC GCUACGGUGA AGGUAACCUC UAUUGUGCAC ACAUUGCCCG UCACCUCCGA UAAUAGUAUU GUACAGGAAG AAC UAUGGC UACACUUAGU CGCGGCCUGG AACGUAUGCG UGAUAUUAGA GUUGGAGUAA GUCGUAACAG GUUGGGGUAG GGGA ACCUG CUCCAGAGUC

Macromolecule #21: S2 rRNA

• Macromolecule: Name: S2 rRNA / type: rna / ID: 21 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 68.527445 KDa

Sequence

String:

UGCUAGGUGA CCCAAUACGG GCAUCGGGCA AAACUCGCGU AGGAUUAGCG AGCUGGGUCG CUUUUUUUUU UCAGCGGCCC AUGGCUUAU CCUUAGCCUG UCUUAACGGU ACUAGGCCAC GGUGGCACUG AAAAGGGGCC ACGGUUCUUA UGAACCCAGC A GUGUUGAA UUUUGGACAA UCGCUUGAAC GGCGGAUCCA GAUGCUGCUU ACAAA

Macromolecule #22: S3 rRNA

• Macromolecule: Name: S3 rRNA / type: rna / ID: 22 / Number of copies: 1
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 126.928117 KDa

Sequence

String:

AUUGUUUGAA CACCCCCCAA GCACGUGCCA GAAGGGUCGG UAAAACGUGC GGUGUCAGUA UAAAGCGUCU UGACUAGGCA GGCAGCGCG UCUGAGCGUG UGAACACUUU AAACGUUGGG UAAUAUUCGG AGGAUCGGUC AAAUGAGAAU AUUCCGGAUG G AAAGCCGA AGGCGAAAGC ACCAACAUCA GAGUCACUAA AGCUUCAACG CGUAAGUUUG GGUAGCGAAC CGGAUUAGAG AC CCGGGUA GUCCAAACCG UCAACACAUU AUAGUAAUCU AUAACGCCUG GUGAUACGGU GGCAACACUA UAAAUCAAAG CAA UUGGCA GCGAUAGAGA UGCGCGGUGG AAUAUGCUGU UUAAAUCGAA UUUACGCGCA AAAUCUUACC ACUUUUU

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 2 mg/mL
• Buffer: pH: 7.6
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TALOS ARCTICA
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
• Sample stage: Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 45.0 e/Ų
• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL
• Initial angle assignment: Type: RANDOM ASSIGNMENT / Software - Name: RELION
• Final 3D classification: Number classes: 6 / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final reconstruction: Number classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 40131