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- EMDB-11457: Mbf1-ribosome complex -

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Basic information

Entry
Database: EMDB / ID: EMD-11457
TitleMbf1-ribosome complex
Map dataMap of Mbf1 bound to 80S ribosomes, (local resolution filtered).
Sample
  • Complex: Mbf1-ribosome complex
    • RNA: x 4 types
    • Protein or peptide: x 34 types
  • Ligand: x 1 types
Keywordstranslation / frameshifting / collision / RIBOSOME
Function / homology
Function and homology information


maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / ribosomal subunit / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Protein methylation / RMTs methylate histone arginines / positive regulation of translational fidelity / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation ...maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / ribosomal subunit / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Protein methylation / RMTs methylate histone arginines / positive regulation of translational fidelity / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation / GDP-dissociation inhibitor activity / : / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ribosomal small subunit export from nucleus / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / positive regulation of protein kinase activity / rescue of stalled ribosome / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / cellular response to amino acid starvation / ribosome assembly / small-subunit processome / cytosolic ribosome / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / ribosome binding / ribosome biogenesis / small ribosomal subunit / cytoplasmic translation / negative regulation of translation / transcription coactivator activity / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / ribonucleoprotein complex / G protein-coupled receptor signaling pathway / negative regulation of gene expression / mRNA binding / ubiquitin protein ligase binding / nucleolus / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Multiprotein bridging factor 1, N-terminal / Multiprotein bridging factor 1 / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / : / : / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 ...Multiprotein bridging factor 1, N-terminal / Multiprotein bridging factor 1 / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / : / : / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / Lambda repressor-like, DNA-binding domain superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S26e signature. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S17e, conserved site / Ribosomal protein S25 / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein S21e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S30 / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S11, N-terminal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / Ribosomal protein S4e, N-terminal, conserved site / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S19e signature. / Ribosomal S17 / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S17, archaeal/eukaryotic / 40S Ribosomal protein S10 / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S3Ae / Plectin/S10, N-terminal / Ribosomal S3Ae family / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S17e signature. / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal protein S7e signature. / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein S4e signature. / Ribosomal protein S8e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e
Similarity search - Domain/homology
RPS5 isoform 1 / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / RPS31 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / 40S ribosomal protein S3 ...RPS5 isoform 1 / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / RPS31 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / 40S ribosomal protein S3 / RPS15 isoform 1 / RPS28A isoform 1 / BJ4_G0052170.mRNA.1.CDS.1 / 40S ribosomal protein S12 / Small ribosomal subunit protein uS4A / Multiprotein-bridging factor 1 / Small ribosomal subunit protein eS17A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Small ribosomal subunit protein RACK1 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS10A / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsBest KM / Denk T
Funding support Germany, United States, 3 items
OrganizationGrant numberCountry
German Research Foundation (DFG) Germany
Howard Hughes Medical Institute (HHMI) United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR) United States
CitationJournal: Elife / Year: 2020
Title: EDF1 coordinates cellular responses to ribosome collisions.
Authors: Niladri K Sinha / Alban Ordureau / Katharina Best / James A Saba / Boris Zinshteyn / Elayanambi Sundaramoorthy / Amit Fulzele / Danielle M Garshott / Timo Denk / Matthias Thoms / Joao A ...Authors: Niladri K Sinha / Alban Ordureau / Katharina Best / James A Saba / Boris Zinshteyn / Elayanambi Sundaramoorthy / Amit Fulzele / Danielle M Garshott / Timo Denk / Matthias Thoms / Joao A Paulo / J Wade Harper / Eric J Bennett / Roland Beckmann / Rachel Green /
Abstract: Translation of aberrant mRNAs induces ribosomal collisions, thereby triggering pathways for mRNA and nascent peptide degradation and ribosomal rescue. Here we use sucrose gradient fractionation ...Translation of aberrant mRNAs induces ribosomal collisions, thereby triggering pathways for mRNA and nascent peptide degradation and ribosomal rescue. Here we use sucrose gradient fractionation combined with quantitative proteomics to systematically identify proteins associated with collided ribosomes. This approach identified Endothelial differentiation-related factor 1 (EDF1) as a novel protein recruited to collided ribosomes during translational distress. Cryo-electron microscopic analyses of EDF1 and its yeast homolog Mbf1 revealed a conserved 40S ribosomal subunit binding site at the mRNA entry channel near the collision interface. EDF1 recruits the translational repressors GIGYF2 and EIF4E2 to collided ribosomes to initiate a negative-feedback loop that prevents new ribosomes from translating defective mRNAs. Further, EDF1 regulates an immediate-early transcriptional response to ribosomal collisions. Our results uncover mechanisms through which EDF1 coordinates multiple responses of the ribosome-mediated quality control pathway and provide novel insights into the intersection of ribosome-mediated quality control with global transcriptional regulation.
History
DepositionJul 24, 2020-
Header (metadata) releaseSep 9, 2020-
Map releaseSep 9, 2020-
UpdateMay 1, 2024-
Current statusMay 1, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6zvi
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6zvi
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11457.png

Downloads & links

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Map

FileDownload / File: emd_11457.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMap of Mbf1 bound to 80S ribosomes, (local resolution filtered).
Voxel sizeX=Y=Z: 1.059 Å
Density
Contour LevelBy AUTHOR: 0.06 / Movie #1: 0.06
Minimum - Maximum-0.0897217 - 0.29753214
Average (Standard dev.)0.0020701867 (±0.014140037)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 423.6 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0591.0591.059
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z423.600423.600423.600
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ500500500
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0900.2980.002

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Supplemental data

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Mask #1

Fileemd_11457_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Post processed map, bFactor -30

Fileemd_11457_additional.map
AnnotationPost processed map, bFactor -30
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 1 of the 3D refinement

Fileemd_11457_half_map_1.map
AnnotationHalf map 1 of the 3D refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 2 of the 3D refinement

Fileemd_11457_half_map_2.map
AnnotationHalf map 2 of the 3D refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mbf1-ribosome complex

EntireName: Mbf1-ribosome complex
Components
  • Complex: Mbf1-ribosome complex
    • RNA: A/P-site tRNA
    • RNA: P/E-site tRNA
    • RNA: mRNAMessenger RNA
    • RNA: 18S rRNA18S ribosomal RNA
    • Protein or peptide: 40S ribosomal protein S0-ARibosome
    • Protein or peptide: RPS1A isoform 1
    • Protein or peptide: RPS2 isoform 1
    • Protein or peptide: RPS3 isoform 1
    • Protein or peptide: 40S ribosomal protein S4-ARibosome
    • Protein or peptide: Rps5p
    • Protein or peptide: 40S ribosomal protein S6-ARibosome
    • Protein or peptide: 40S ribosomal protein S7-ARibosome
    • Protein or peptide: 40S ribosomal protein S8-ARibosome
    • Protein or peptide: 40S ribosomal protein S9-ARibosome
    • Protein or peptide: 40S ribosomal protein S10-ARibosome
    • Protein or peptide: 40S ribosomal protein S11-ARibosome
    • Protein or peptide: RPS12 isoform 1
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: RPS15 isoform 1
    • Protein or peptide: 40S ribosomal protein S16-ARibosome
    • Protein or peptide: 40S ribosomal protein S17-ARibosome
    • Protein or peptide: 40S ribosomal protein S18-ARibosome
    • Protein or peptide: 40S ribosomal protein S19-ARibosome
    • Protein or peptide: RPS20 isoform 1
    • Protein or peptide: 40S ribosomal protein S21-ARibosome
    • Protein or peptide: RPS22A isoform 1
    • Protein or peptide: 40S ribosomal protein S23-ARibosome
    • Protein or peptide: 40S ribosomal protein S24-ARibosome
    • Protein or peptide: RPS25A isoform 1
    • Protein or peptide: 40S ribosomal protein S27-ARibosome
    • Protein or peptide: RPS28A isoform 1
    • Protein or peptide: 40S ribosomal protein S30-ARibosome
    • Protein or peptide: RPS31 isoform 1
    • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Protein or peptide: MBF1 isoform 1
    • Protein or peptide: RPS29A isoform 1
    • Protein or peptide: 40S ribosomal protein S14-BRibosome
    • Protein or peptide: RPS26B isoform 1
  • Ligand: ZINC ION

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Supramolecule #1: Mbf1-ribosome complex

SupramoleculeName: Mbf1-ribosome complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#38
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: A/P-site tRNA

MacromoleculeName: A/P-site tRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.501539 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUAGGAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #2: P/E-site tRNA

MacromoleculeName: P/E-site tRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.802785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

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Macromolecule #3: mRNA

MacromoleculeName: mRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 10.947276 KDa
SequenceString:
UUUUUUUUUU UUGACAAAUU UUUUUUUUAA ACUGG

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Macromolecule #4: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 566.313438 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCGGAU UUCCAACGGG GCCUUUCCUU CUGGCUAACC UUGAGUCCUU GUGGCUCUUG GCGAACCA G GACUUUUACU UUGAAAAAAU UAGAGUGUUC AAAGCAGGCG UAUUGCUCGA AUAUAUUAGC AUGGAAUAAU AGAAUAGGA CGUUUGGUUC UAUUUUGUUG GUUUCUAGGA CCAUCGUAAU GAUUAAUAGG GACGGUCGGG GGCAUCAGUA UUCAAUUGUC AGAGGUGAA AUUCUUGGAU UUAUUGAAGA CUAACUACUG CGAAAGCAUU UGCCAAGGAC GUUUUCAUUA AUCAAGAACG A AAGUUAGG GGAUCGAAGA UGAUCAGAUA CCGUCGUAGU CUUAACCAUA AACUAUGCCG ACUAGGGAUC GGGUGGUGUU UU UUUAAUG ACCCACUCGG CACCUUACGA GAAAUCAAAG UCUUUGGGUU CUGGGGGGAG UAUGGUCGCA AGGCUGAAAC UUA AAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACACGGGGAA ACUCACCAGG UCCA GACAC AAUAAGGAUU GACAGAUUGA GAGCUCUUUC UUGAUUUUGU GGGUGGUGGU GCAUGGCCGU UCUUAGUUGG UGGAG UGAU UUGUCUGCUU AAUUGCGAUA ACGAACGAGA CCUUAACCUA CUAAAUAGUG GUGCUAGCAU UUGCUGGUUA UCCACU UCU UAGAGGGACU AUCGGUUUCA AGCCGAUGGA AGUUUGAGGC AAUAACAGGU CUGUGAUGCC CUUAGACGUU CUGGGCC GC ACGCGCGCUA CACUGACGGA GCCAGCGAGU CUAACCUUGG CCGAGAGGUC UUGGUAAUCU UGUGAAACUC CGUCGUGC U GGGGAUAGAG CAUUGUAAUU AUUGCUCUUC AACGAGGAAU UCCUAGUAAG CGCAAGUCAU CAGCUUGCGU UGAUUACGU CCCUGCCCUU UGUACACACC GCCCGUCGCU AGUACCGAUU GAAUGGCUUA GUGAGGCCUC AGGAUCUGCA GAGCGGAGAA UUUGGACAA ACUUGGUCAU UUAGAGGAAC UAAAAGUCGU AACAAGGUUU CCGUAGGUGA ACCUGCGGAA GGAUCAUUA

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Macromolecule #5: 40S ribosomal protein S0-A

MacromoleculeName: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.811074 KDa
SequenceString: SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS ...String:
SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDP

UniProtKB: Small ribosomal subunit protein uS2A

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Macromolecule #6: RPS1A isoform 1

MacromoleculeName: RPS1A isoform 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.498365 KDa
SequenceString: VVDPFTRKEW FDIKAPSTFE NRNVGKTLVN KSTGLKSASD ALKGRVVEVC LADLQGSEDH SFRKIKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRHSYAQS SHIRAIRKVI SEILTKEVQG S TLAQLTSK ...String:
VVDPFTRKEW FDIKAPSTFE NRNVGKTLVN KSTGLKSASD ALKGRVVEVC LADLQGSEDH SFRKIKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRHSYAQS SHIRAIRKVI SEILTKEVQG S TLAQLTSK LIPEVINKEI ENATKDIFPL QNIHVRKVKL LKQPKFDVGA LMALHGEG

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #7: RPS2 isoform 1

MacromoleculeName: RPS2 isoform 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 23.212979 KDa
SequenceString: GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN ...String:
GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASAQ

UniProtKB: RPS2 isoform 1

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Macromolecule #8: RPS3 isoform 1

MacromoleculeName: RPS3 isoform 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.702791 KDa
SequenceString: ALISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEVIIRA TRTQDVLGEN GRRINELTLL VQKRFKYAPG TIVLYAERV QDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVVSGK LRAARAKAMK FADGFLIHSG Q PVNDFIDT ...String:
ALISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEVIIRA TRTQDVLGEN GRRINELTLL VQKRFKYAPG TIVLYAERV QDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVVSGK LRAARAKAMK FADGFLIHSG Q PVNDFIDT ATRHVLMRQG VLGIKVKIMR DPAKSRTGPK ALPDAVTIIE PKEEEPILAP SVKDY

UniProtKB: 40S ribosomal protein S3

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Macromolecule #9: 40S ribosomal protein S4-A

MacromoleculeName: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 29.338133 KDa
SequenceString: ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI ...String:
ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ QGL

UniProtKB: Small ribosomal subunit protein eS4A

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Macromolecule #10: Rps5p

MacromoleculeName: Rps5p / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.908338 KDa
SequenceString: FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI ...String:
FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

UniProtKB: RPS5 isoform 1

+
Macromolecule #11: 40S ribosomal protein S6-A

MacromoleculeName: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.965975 KDa
SequenceString: MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT ...String:
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE

UniProtKB: Small ribosomal subunit protein eS6A

+
Macromolecule #12: 40S ribosomal protein S7-A

MacromoleculeName: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.07157 KDa
SequenceString:
APQAKILSQA PTELELQVAQ AFVELENSSP ELKAELRPLQ FKSIREIDVA GGKKALAIFV PVPSLAGFHK VQTKLTRELE KKFQDRHVI FLAERRILPK PSRTSRQVQK RPRSRTLTAV HDKILEDLVF PTEIVGKRVR YLVGGNKIQK VLLDSKDVQQ I DYKLESFQ AVYNKLTGKQ IVFEIPS

UniProtKB: Small ribosomal subunit protein eS7A

+
Macromolecule #13: 40S ribosomal protein S8-A

MacromoleculeName: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.406609 KDa
SequenceString: GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF S AGRLYACI ...String:
GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF S AGRLYACI SSRPGQSGRC DGYILEGEEL AFYLRRLTAK K

UniProtKB: Small ribosomal subunit protein eS8A

+
Macromolecule #14: 40S ribosomal protein S9-A

MacromoleculeName: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.210662 KDa
SequenceString:
PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASGE

UniProtKB: Small ribosomal subunit protein uS4A

+
Macromolecule #15: 40S ribosomal protein S10-A

MacromoleculeName: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 11.042562 KDa
SequenceString:
MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYI

UniProtKB: Small ribosomal subunit protein eS10A

+
Macromolecule #16: 40S ribosomal protein S11-A

MacromoleculeName: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.588461 KDa
SequenceString:
STELTVQSER AFQKQPHIFN NPKVKTSKRT KRWYKNAGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTKMH RTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVV KVSAAAG

UniProtKB: Small ribosomal subunit protein uS17A

+
Macromolecule #17: RPS12 isoform 1

MacromoleculeName: RPS12 isoform 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.327331 KDa
SequenceString:
AEVTIEDALK VVLRTALVHD GLARGLREST KALTRGEALL VVLVSSVTEA NIIKLVEGLA NDPENKVPLI KVADAKQLGE WAGLGKIDR EGNARKVVGA SVVVVKNWGA ETDELSMIME HFSQQ

UniProtKB: 40S ribosomal protein S12

+
Macromolecule #18: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.928748 KDa
SequenceString:
GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

UniProtKB: Small ribosomal subunit protein uS15

+
Macromolecule #19: RPS15 isoform 1

MacromoleculeName: RPS15 isoform 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.421732 KDa
SequenceString:
THSYRGVDLE KLLEMSTEDF VKLAPARVRR RFARGMTSKP AGFMKKLRAA KLAAPENEKP APVRTHMRNM IIVPEMIGSV VGIYNGKAF NQVEIRPEML GHYLGEFSIT YTPVRHGRAG

UniProtKB: RPS15 isoform 1

+
Macromolecule #20: 40S ribosomal protein S16-A

MacromoleculeName: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.659216 KDa
SequenceString:
AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

UniProtKB: Small ribosomal subunit protein uS9A

+
Macromolecule #21: 40S ribosomal protein S17-A

MacromoleculeName: 40S ribosomal protein S17-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.268545 KDa
SequenceString:
GRVRTKTVKR ASKALIERYY PKLTLDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPEVSA LDLSRSNGVL NVDNQTSDLV KSLGLKLPLS VINVSA

UniProtKB: Small ribosomal subunit protein eS17A

+
Macromolecule #22: 40S ribosomal protein S18-A

MacromoleculeName: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.940443 KDa
SequenceString:
SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

UniProtKB: Small ribosomal subunit protein uS13A

+
Macromolecule #23: 40S ribosomal protein S19-A

MacromoleculeName: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.81093 KDa
SequenceString:
PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

UniProtKB: Small ribosomal subunit protein eS19A

+
Macromolecule #24: RPS20 isoform 1

MacromoleculeName: RPS20 isoform 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 11.476479 KDa
SequenceString:
IIKIRITLTS TKVKQLENVS SNIVKNAEQH NLVKKGPVRL PTKVLKISTR KTPNGEGSKT WETYEMRIHK RYIDLEAPVQ IVKRITQIT IEPGVDVEVV VA

UniProtKB: RPS20 isoform 1

+
Macromolecule #25: 40S ribosomal protein S21-A

MacromoleculeName: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 9.758829 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21A

+
Macromolecule #26: RPS22A isoform 1

MacromoleculeName: RPS22A isoform 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.518867 KDa
SequenceString:
TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

UniProtKB: RPS22A isoform 1

+
Macromolecule #27: 40S ribosomal protein S23-A

MacromoleculeName: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.942699 KDa
SequenceString:
GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

UniProtKB: Small ribosomal subunit protein uS12A

+
Macromolecule #28: 40S ribosomal protein S24-A

MacromoleculeName: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.23165 KDa
SequenceString:
SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

UniProtKB: Small ribosomal subunit protein eS24A

+
Macromolecule #29: RPS25A isoform 1

MacromoleculeName: RPS25A isoform 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 7.930299 KDa
SequenceString:
QHAVILDQEK YDRILKEVPT YRYVSVSVLV DRLKIGGSLA RIALRHLEKE GIIKPISKHS KQAIYTRAT

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #30: 40S ribosomal protein S27-A

MacromoleculeName: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 8.762195 KDa
SequenceString:
VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

UniProtKB: Small ribosomal subunit protein eS27A

+
Macromolecule #31: RPS28A isoform 1

MacromoleculeName: RPS28A isoform 1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 7.116281 KDa
SequenceString:
TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

UniProtKB: RPS28A isoform 1

+
Macromolecule #32: 40S ribosomal protein S30-A

MacromoleculeName: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 5.267314 KDa
SequenceString:
AKVHGSLARA GKVKSQTPKV EKTEKPKKPK GRAYKRLLYT RRFVNV

UniProtKB: Small ribosomal subunit protein eS30A

+
Macromolecule #33: RPS31 isoform 1

MacromoleculeName: RPS31 isoform 1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 8.329946 KDa
SequenceString:
RKKKVYTTPK KIKHKHKAVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

UniProtKB: RPS31 isoform 1

+
Macromolecule #34: Guanine nucleotide-binding protein subunit beta-like protein

MacromoleculeName: Guanine nucleotide-binding protein subunit beta-like protein
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 34.151426 KDa
SequenceString: LVLRGTLEGH NGWVTSLATS AGQPNLLLSA SRDKTLISWK LTGDDQKFGV PVRSFKGHSH IVQDCTLTAD GAYALSASWD KTLRLWDVA TGETYQRFVG HKSDVMSVDI DKKASMIISG SRDKTIKVWT IKGQCLATLL GHNDWVSQVR VVPNEAADDD S VTIISAGN ...String:
LVLRGTLEGH NGWVTSLATS AGQPNLLLSA SRDKTLISWK LTGDDQKFGV PVRSFKGHSH IVQDCTLTAD GAYALSASWD KTLRLWDVA TGETYQRFVG HKSDVMSVDI DKKASMIISG SRDKTIKVWT IKGQCLATLL GHNDWVSQVR VVPNEAADDD S VTIISAGN DKMVKAWNLN QFQIEADFIG HNSNINTLTA SPDGTLIASA GKDGEIMLWN LAAKKAMYTL SAQDEVFSLA FS PNRYWLA AATATGIKVF SLDPQYLVDD LRPEFAGYSK AAEPHAVSLA WSADGQTLFA GYTDNVIRVW QVMTAN

UniProtKB: Small ribosomal subunit protein RACK1

+
Macromolecule #35: MBF1 isoform 1

MacromoleculeName: MBF1 isoform 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.423187 KDa
SequenceString:
RSQGQINAAR RQGLVVSVDK KYGSTNTRGD NEGQRLTKVD RETDIVKPKK LDPNVGRAIS RARTDKKMSQ KDLATKINEK PTVVNDYEA ARAIPNQQVL SKLERALGVK LR

UniProtKB: BJ4_G0052170.mRNA.1.CDS.1

+
Macromolecule #36: RPS29A isoform 1

MacromoleculeName: RPS29A isoform 1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 6.335303 KDa
SequenceString:
ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

UniProtKB: RPS29A isoform 1

+
Macromolecule #37: 40S ribosomal protein S14-B

MacromoleculeName: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.560528 KDa
SequenceString:
NSQVFGVARI YASFNDTFVH VTDLSGKETI ARVTGGMKVK ADRDESSPYA AMLAAQDVAA KCKEVGITAV HVKIRATGGT RTKTPGPGG QAALRALARS GLRIGRIEDV TPVPSDSTRK KGGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11B

+
Macromolecule #38: RPS26B isoform 1

MacromoleculeName: RPS26B isoform 1 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 11.022989 KDa
SequenceString:
PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #39: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 39 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 28.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 57350
FSC plot (resolution estimation)

+
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