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- EMDB-10958: The structure of the small subunit of the mitoribosome from Neuro... -

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Basic information

Entry
Database: EMDB / ID: EMD-10958
TitleThe structure of the small subunit of the mitoribosome from Neurospora crassa
Map dataComposite map made out of following maps: head, body and tail.
Sample
  • Complex: Mitoribosome of Neurospora crassa
    • Protein or peptide: x 35 types
    • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


3-hydroxyisobutyryl-CoA hydrolase activity / nuclear pore inner ring / valine catabolic process / nuclear pore organization / ATPase inhibitor activity / negative regulation of ATP-dependent activity / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / mitochondrial small ribosomal subunit / structural constituent of nuclear pore ...3-hydroxyisobutyryl-CoA hydrolase activity / nuclear pore inner ring / valine catabolic process / nuclear pore organization / ATPase inhibitor activity / negative regulation of ATP-dependent activity / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / mitochondrial small ribosomal subunit / structural constituent of nuclear pore / mitochondrial translation / superoxide dismutase activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / small ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / metal ion binding / cytoplasm
Similarity search - Function
: / Ribosomal protein L31p, N-terminal / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial ...: / Ribosomal protein L31p, N-terminal / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / IGR protein motif / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / ClpP/crotonase-like domain superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Sterile alpha motif/pointed domain superfamily / Ribosomal protein S21 / Ribosomal protein S13, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S19 / Ribosomal protein S2 signature 1. / Ribosomal protein S13, conserved site / Ribosomal protein S2, conserved site / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S14 / Ribosomal protein S2 / Ribosomal protein S4, conserved site / Ribosomal protein S13/S18 / Ribosomal protein S4/S9 / Ribosomal protein S19 signature. / Ribosomal protein S14p/S29e / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S10p/S20e / Ribosomal protein S13-like, H2TH / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S8 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / S4 RNA-binding domain / Ribosomal protein S11 / RNA-binding S4 domain / Ribosomal protein S13 signature. / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / RNA-binding S4 domain superfamily / Ribosomal protein S13 family profile.
Similarity search - Domain/homology
37S ribosomal protein mrp5 / Ribosomal protein S16, mitochondrial / Ribosomal protein S5, mitochondrial / 37S ribosomal protein S5 / Ribosomal protein S15 / Mitochondrial 37S ribosomal protein S27 / 37S ribosomal protein S24, mitochondrial / Protein FYV4, mitochondrial / Mitochondrial 3-hydroxyisobutyryl-CoA hydrolase / Large ribosomal subunit protein bL31m ...37S ribosomal protein mrp5 / Ribosomal protein S16, mitochondrial / Ribosomal protein S5, mitochondrial / 37S ribosomal protein S5 / Ribosomal protein S15 / Mitochondrial 37S ribosomal protein S27 / 37S ribosomal protein S24, mitochondrial / Protein FYV4, mitochondrial / Mitochondrial 3-hydroxyisobutyryl-CoA hydrolase / Large ribosomal subunit protein bL31m / Mito ribosomal protein S18 / Mitochondrial ribosomal protein S19 / 37S ribosomal protein S10, mitochondrial / Small ribosomal subunit protein mS27 / 40S ribosomal protein S13 / Fe superoxide dismutase / Mitochondrial 37S ribosomal protein S17 / 37S ribosomal protein mrp10, mitochondrial / Ribosomal_S7 domain-containing protein / 37S ribosomal protein S9, mitochondrial / Mitochondrial 30S ribosomal protein S12 / Mito ribosomal protein S4 / Mito ribosomal protein S21 / Mito ribosomal protein S6 / Small ribosomal subunit protein mS29 / Mitochondrial 40S ribosomal protein MRP2 / Protein FYV4, mitochondrial / Mitochondrial ribosomal protein subunit S18 / Small ribosomal subunit protein mS45 / 40S ribosomal protein S8 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS2m / ATP synthase F1 subunit epsilon
Similarity search - Component
Biological speciesNeurospora crassa OR74A (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.85 Å
AuthorsAmunts A / Itoh Y / Naschberger A
Funding support Sweden, European Union, 4 items
OrganizationGrant numberCountry
Swedish Research CouncilNT_2015-04107 Sweden
Knut and Alice Wallenberg Foundation2018.0080 Sweden
European Research Council (ERC)(ERC-2018-StG-805230European Union
European Molecular Biology Organization (EMBO)H2020-MSCA-IF-2017European Union
CitationJournal: Nat Commun / Year: 2020
Title: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.
Authors: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts /
Abstract: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
History
DepositionApr 29, 2020-
Header (metadata) releaseNov 11, 2020-
Map releaseNov 11, 2020-
UpdateNov 11, 2020-
Current statusNov 11, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3.74
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 3.74
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6yw5
  • Surface level: 3.74
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6yw5
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10958.png

Downloads & links

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Map

FileDownload / File: emd_10958.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map made out of following maps: head, body and tail.
Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 3.74 / Movie #1: 3.74
Minimum - Maximum-22.282217 - 49.86823
Average (Standard dev.)0.0019076765 (±1.1022679)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 423.99997 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z424.000424.000424.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ400400400
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-22.28249.8680.002

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Supplemental data

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Mask #1

Fileemd_10958_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Consensus map for the SSU of the mitoribosome from Neurospora crassa

Fileemd_10958_additional_1.map
AnnotationConsensus map for the SSU of the mitoribosome from Neurospora crassa
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_10958_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_10958_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mitoribosome of Neurospora crassa

EntireName: Mitoribosome of Neurospora crassa
Components
  • Complex: Mitoribosome of Neurospora crassa
    • Protein or peptide: uS2m
    • Protein or peptide: Mito ribosomal protein S2
    • Protein or peptide: Ribosomal protein S5, mitochondrialRibosome
    • Protein or peptide: Mito ribosomal protein S4
    • Protein or peptide: 37S ribosomal protein S5
    • Protein or peptide: Mito ribosomal protein S6
    • Protein or peptide: Ribosomal_S7 domain-containing protein
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: uS9m
    • Protein or peptide: 37S ribosomal protein S10, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein subunit S18
    • Protein or peptide: Mitochondrial 30S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: Mitochondrial 40S ribosomal protein MRP2
    • Protein or peptide: Ribosomal protein S15
    • Protein or peptide: Ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: Mitochondrial 37S ribosomal protein S17
    • Protein or peptide: Mito ribosomal protein S18
    • Protein or peptide: Mitochondrial ribosomal protein S19
    • Protein or peptide: Mito ribosomal protein S21
    • Protein or peptide: 37S ribosomal protein S25, mitochondrialRibosome
    • Protein or peptide: mS26
    • Protein or peptide: mS27
    • Protein or peptide: Mitochondrial ribosomal protein DAP3
    • Protein or peptide: mS33
    • Protein or peptide: 37S ribosomal protein S24, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein mrp10, mitochondrialRibosome
    • Protein or peptide: DUF1713 domain-containing protein
    • Protein or peptide: IGR domain-containing protein
    • Protein or peptide: Fe superoxide dismutase
    • Protein or peptide: mS42
    • Protein or peptide: mS46
    • Protein or peptide: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
    • Protein or peptide: mS47
    • RNA: 16S rRNA
    • Protein or peptide: IF1
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: POTASSIUM IONPotassium

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Supramolecule #1: Mitoribosome of Neurospora crassa

SupramoleculeName: Mitoribosome of Neurospora crassa / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Source (natural)Organism: Neurospora crassa OR74A (fungus)

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Macromolecule #1: uS2m

MacromoleculeName: uS2m / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 51.59634 KDa
SequenceString: MAFSRGVSPG GALLRTSRMF SLPPVIPPPP GNKLQMISER ASATEAYPTH QVLTTFESSR SRGDWGLKRP LPLKSTTGTT YPMVKVKEM DSLEQITDFT SGTQHGLTLK KFQALNIPIS TPSEISDPSR LFRPVQRSVF EADTDVTAFS PDEQIQEAEK R WKFSGPWL ...String:
MAFSRGVSPG GALLRTSRMF SLPPVIPPPP GNKLQMISER ASATEAYPTH QVLTTFESSR SRGDWGLKRP LPLKSTTGTT YPMVKVKEM DSLEQITDFT SGTQHGLTLK KFQALNIPIS TPSEISDPSR LFRPVQRSVF EADTDVTAFS PDEQIQEAEK R WKFSGPWL AGMTPGEFKE YLAKTVRPKR AEFRKFIQKK IAAQKTEAAN RELQETAALR GDAVAETQEP FKPESITDDE VT EYLRRLR NDNQVLYDLV GQFLDLAPLK PPQAAEARQH SLLVNLRATD SPYGGRGPPI THPSAGISYL RTAAYLNNHP IYG PQKSHP PVQARVLKPR RGGLGNDAKI GVAGFVADGP LGTSHSNLRG NSVMDKFDPS IEGGAKLWVN VDKATVDSTG RVQL TVSDA KATDVLIAKE LIGDAREPIF GSAPKRQEKT FKKIPMTAAR LRGRYENSDS PSSPTMSGSS GYGLR

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Macromolecule #2: Mito ribosomal protein S2

MacromoleculeName: Mito ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 47.197305 KDa
SequenceString: MIVRNIGARL GRRALATPLS RASFPAQSRF LSQDNFTAPP PPPTNSKKQA AKTAFAEPTL EEQAEFYAES IEKHTPEFAA SPAAEAWSA PSATATETVT TWDPSLVDEE ALKLKQQIEA IPGNFKLFKQ TKAQTQKLGA GVEVRYIPEQ YLRNPPSDAS L EDLMAAQA ...String:
MIVRNIGARL GRRALATPLS RASFPAQSRF LSQDNFTAPP PPPTNSKKQA AKTAFAEPTL EEQAEFYAES IEKHTPEFAA SPAAEAWSA PSATATETVT TWDPSLVDEE ALKLKQQIEA IPGNFKLFKQ TKAQTQKLGA GVEVRYIPEQ YLRNPPSDAS L EDLMAAQA HMGHNTSLWN PANARYIYGV RQGIHIISLE TTATHLRRAA RVVEEVAYRG GLILFVGTRP GQRPIVVRAA EL AKACHLF TKWRPGTITN REQLLGGVPL TVVDELDRPL SGFEDHLHDR RPLAPDLVVC LNPKENMTLL YECSLAKIPT IGI IDTNTN PSWVTYQIPA NDDSLRATAL ISGVLGRAGE RGQKRRLEAA QRGVVTWKTP ADVQGYFELA SARAADARRR QAQD NSVEE QKEKDTFLSE DALKAMFGGD ARI

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Macromolecule #3: Ribosomal protein S5, mitochondrial

MacromoleculeName: Ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 58.907285 KDa
SequenceString: MNNTNTNTNN KNLASSSASV IFSKYINNNN NKLIPFKIKN SDLGRTRYFP PISKEWKNSI YVFNHNNLKN LPLFDININS LIKDYFNLQ FKDKILFKKK RLSKVKVVSL NKIYASKAEI KHTNTKAILT VYTFNREKIS LYKKIKKLKK SFYFVFDKII S FSERVILS ...String:
MNNTNTNTNN KNLASSSASV IFSKYINNNN NKLIPFKIKN SDLGRTRYFP PISKEWKNSI YVFNHNNLKN LPLFDININS LIKDYFNLQ FKDKILFKKK RLSKVKVVSL NKIYASKAEI KHTNTKAILT VYTFNREKIS LYKKIKKLKK SFYFVFDKII S FSERVILS GVPIRVDKDG KVLSNLYLPF RLRGVLPWIT ESHVNIWRKI IIASLYKELI LLRKYKLRLD LNKYKFEEKL LY RLNNLIM KYYNKKVEFN IVNMRSFLLN SDILTKILAL KLKNRNARVI KIMDVILNKA NLPKINRVQE KASLIKSVDW NLL ENKFKN LNLSFILNDA SYAERNNLSE LLNKLYYNVL LVSQKGVWAL RSPKGEQPSF HSKSGGSPTK FIQKGAGAAS LGGN AKAQP KVSSFAKAKK YAKIYQIIFN SINYKNMGGL RLEIKGRLTK RYRADRSLFK VKWKGGLKNL DSSYKGLSSV NMRGY AKPN VEYSIFTSKR RIGAFAVKGW VSGK

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Macromolecule #4: Mito ribosomal protein S4

MacromoleculeName: Mito ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 51.610031 KDa
SequenceString: MKIRRLFKYH SLKKKPIRPT WNKYNLYNLA TAGREPRISG KTFFQQKWLA KSLTRAYHGE HIKERKWARM FSRRLPAVVN MDPAYMAKY NGSEQAAGRG SGLSEPPAYE KTADEKPAKQ VIANPGRKVP TPYEQMTFAP LERRLDIAIF RALFASSARQ A RQMVVHGA ...String:
MKIRRLFKYH SLKKKPIRPT WNKYNLYNLA TAGREPRISG KTFFQQKWLA KSLTRAYHGE HIKERKWARM FSRRLPAVVN MDPAYMAKY NGSEQAAGRG SGLSEPPAYE KTADEKPAKQ VIANPGRKVP TPYEQMTFAP LERRLDIAIF RALFASSARQ A RQMVVHGA VTVNGKKMKH PGYLLNPGDL FQVDVERVLY ATGAPKDKKL LAAAMKAAKK ELAEEAQEAA EEKDAEAEEA EE GEAQEAA SKSGKKEKQV DTSKFDEAWN RKYELLQLQR LLQYAKHIAD SPSHQLSATR KQALRALKRD IKAAIKQAQR RDG SVSPTG NVVDDLTMLL ERLAVSPSKA IQQEEAAKEL SKKLDKQSRQ VLKKVIEYND EHEDNEIDPS KPYMTPWRPR NYMS AFAFI PRYLEVNQNI CAAVYLRHPV ARPGESEVPS PFSPTINQLA FNWYLRRG

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Macromolecule #5: 37S ribosomal protein S5

MacromoleculeName: 37S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 53.297543 KDa
SequenceString: MSAARFAARS FLSRRLAATV PAAAPAASTP AASTCPAAQQ QQSQFHSSAH LEARRRSRFK NVRAVEMGLT SDAKIESFTK KKFAEYTED EKAALAHNYP AEHMEAIEAA EAAIDPKDLT IQGRLRVDPY RMPYIDDFSE IQPIIDKRAR RSAPPSHKAR F MDVDEFTQ ...String:
MSAARFAARS FLSRRLAATV PAAAPAASTP AASTCPAAQQ QQSQFHSSAH LEARRRSRFK NVRAVEMGLT SDAKIESFTK KKFAEYTED EKAALAHNYP AEHMEAIEAA EAAIDPKDLT IQGRLRVDPY RMPYIDDFSE IQPIIDKRAR RSAPPSHKAR F MDVDEFTQ DLINWADEIR RGEPTHRMKK LRDFVPEEFF EKPEGQWPKD VRDEAFTKFW AYLKEQKDAD AKAAANATGP TD GDILSYI LERSSMTDNN LQANSSLAPA LPDKVPGVEG KYRNAIDPAD DGLDDKGQYQ ELKKRTGMSV RQILQLKTKK LVH RRVVNQ TRLGKIASDS VMVIAGNGDG WLGLGMAKSV EASIAVEKAT LLAIQNMQPI PRYENRTIYG EVTTKVSGTI VRLN SRPPG FGLRVSHRIF EMCRAAGIRD LSAKFLRSRN PMNTVKATYQ ALLSQPNPED LAIGRGKKLV DVRKVYYGGS VY

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Macromolecule #6: Mito ribosomal protein S6

MacromoleculeName: Mito ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 13.304392 KDa
SequenceString:
MLYETIGIVR PGNIAEVKEL VLTAGKLILN QGGVIRDIKN WGTFLLPRPI STNQQRHNRG HYFVMRYDAS IATHEEVRRT MKADPRVIR TANVKLGDGK LETLSRFGAI PWRSLEEA

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Macromolecule #7: Ribosomal_S7 domain-containing protein

MacromoleculeName: Ribosomal_S7 domain-containing protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 33.821914 KDa
SequenceString: MPARLGLSAA FRSLSIRTNQ LPQQQSIAAA VQARTLITDS TTSSRLPPRV QIQQQQQQRT QPYSTETTPP PNSNNGDLAG IEGQPPVEV TPENAAALSQ LSEIAYGVKA ADVAIEGHKY GLPTLPLPSE LHHKYRYSEV VNHATKLLMK DGKLSKAQRD M AMILNYLR ...String:
MPARLGLSAA FRSLSIRTNQ LPQQQSIAAA VQARTLITDS TTSSRLPPRV QIQQQQQQRT QPYSTETTPP PNSNNGDLAG IEGQPPVEV TPENAAALSQ LSEIAYGVKA ADVAIEGHKY GLPTLPLPSE LHHKYRYSEV VNHATKLLMK DGKLSKAQRD M AMILNYLR SSPPPKLNPS RPLIPGHPPA SHLPLNPVEY LTVAIDSVAP LIKMRGYKGL AGGGRSLEVP QPIPARQRRS TA FKWILDV VNKKPSKGSG RTMFAHRVAE EIVAVVEGRS SVWDKRMLIH KLGTANRANL NSPALQLKGK RF

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Macromolecule #8: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 17.743707 KDa
SequenceString:
MGIQQITNMC SHLQNASRAR LGLTSLPNTK YNLALALALH RAGFISSITR GGPHPPTPEA LMTYEPEPVT SANVATRRLW VGLKYWNEE PVLKELKPIS KPSRLVTASL EQLNRVARGF PAGYMKGLQL GECLFVNTDR GVLEVREAVE RKVGGLVLCK V K

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Macromolecule #9: uS9m

MacromoleculeName: uS9m / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 35.31957 KDa
SequenceString: MMASLRHSIT SALRSSRQGC SKSAQWQSLD QQFGALRISS RSLSTHAHDH NPNVDGQFIA APALSIDNFK LHPYARAVPV SPSYFTRTP RFYDNYLSLE KLMEEYEDLP VIPATAVERV AWKTLEDIRK ELGEQVKASE FARCLALVKR LNSIHPDLKP Q EIKDVLDS ...String:
MMASLRHSIT SALRSSRQGC SKSAQWQSLD QQFGALRISS RSLSTHAHDH NPNVDGQFIA APALSIDNFK LHPYARAVPV SPSYFTRTP RFYDNYLSLE KLMEEYEDLP VIPATAVERV AWKTLEDIRK ELGEQVKASE FARCLALVKR LNSIHPDLKP Q EIKDVLDS FRRNVQPFTN VAKPIPIDQF GRACGVGKRK ASSARAFVVE GTGEVLVNGK TLAEYFGRVH DRESAVWALR AT NRIDKYN VWVKVEGGGT TGQAEAITLA IAKALLAHEP ALKPALRRAG CVTRDPRKVE RKKHGHVKAR KMPTWVKR

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Macromolecule #10: 37S ribosomal protein S10, mitochondrial

MacromoleculeName: 37S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 30.37707 KDa
SequenceString: MIIRPVVRSL QGRAPLATLR SAQSVFQPLP QLRRNASVTT GPDAASKAST TPIARITTTT EAPKDQKKAK AAAAAAAEPE EQEVIMSRM PRSLEALYLQ PLRREAEYGV PSCDLQLRSY SIRNLEFFCD FALRAAYYLG LPAFGPVPLP RMIERWTVPK S HFIFKKSQ ...String:
MIIRPVVRSL QGRAPLATLR SAQSVFQPLP QLRRNASVTT GPDAASKAST TPIARITTTT EAPKDQKKAK AAAAAAAEPE EQEVIMSRM PRSLEALYLQ PLRREAEYGV PSCDLQLRSY SIRNLEFFCD FALRAAYYLG LPAFGPVPLP RMIERWTVPK S HFIFKKSQ ENFERVTLRR LIQIKDGHPE TVQLWLAFLQ KHAYYGIGMK ANVWEFSKLG VSKAMDESKS EVEKLLETRW EH LSHVSDM KGVGNFEDFL AKERLRISGG R

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Macromolecule #11: Mitochondrial ribosomal protein subunit S18

MacromoleculeName: Mitochondrial ribosomal protein subunit S18 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 42.159832 KDa
SequenceString: MAGQIDPIRA ELVGWTPRVP RNLLASLPPL GRKTAPKLPQ NISFSPPLSA HPCYQCQEKC LPHRRPVSWS PLILQRCRLR CLPRGHPLK ISRTFELPVG ERDGSHPLTA FARAPRTTEP RSLHYLYDSL PPTPALCAQL LFHQLIQGHF SFVVHPTVAT A PRSIWPAK ...String:
MAGQIDPIRA ELVGWTPRVP RNLLASLPPL GRKTAPKLPQ NISFSPPLSA HPCYQCQEKC LPHRRPVSWS PLILQRCRLR CLPRGHPLK ISRTFELPVG ERDGSHPLTA FARAPRTTEP RSLHYLYDSL PPTPALCAQL LFHQLIQGHF SFVVHPTVAT A PRSIWPAK MSRFSTGRLL AQNLFQAFNK PVFPSATPVW TRAFSQTAAR RDADSESSAK LMESLTRGIV GMAADPTDLT GD KLATNIG LRDTEDEPYH FHIYSHKHNT HITVTKPNRD ALISLSCGNL GFKKSNRKHY DSAYQLGAYV VDKMHQMNLH NKI KKMEVV LRGFGPGREA VIKVLLGNEG RMLRSSIVRV SDATRLKFGG TRSKKPRRLG

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Macromolecule #12: Mitochondrial 30S ribosomal protein S12

MacromoleculeName: Mitochondrial 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 18.657877 KDa
SequenceString:
MATTFLRNLF SQAQPSLLRR PLMPSSSSIL PAAARLFSST PAQNATLNQV MRGCRKPQRA RHAVSPALSS IKSPALKGVC VKVGITRPK KPNSGERKTA RVRLSTGKVI TAYIPGEGHN ISQHSVVLVR GGRAQDCPGV RYHLVRGALD LAGVATRMSS R SKYGTKKP KKASVG

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Macromolecule #13: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 13.503821 KDa
SequenceString:
MVFILGVNFN EHKLVQKALE SFYGLGQQAS ARILAKYSIH PRAKMGTLPP KIVTALTAEL STMTIENDAR RLVLDNIKRL RDMGTYRGR RHAMGLPVRG QQTRNQIANA RKLNKIERHG

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Macromolecule #14: Mitochondrial 40S ribosomal protein MRP2

MacromoleculeName: Mitochondrial 40S ribosomal protein MRP2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 13.210644 KDa
SequenceString:
MSMFRAKKLD LGCFTNVRVL RDHSKRKAFL EAEPERQALR YVIRNTTLPA RTRAVAQLQL TQMHAYTRPT QIRNRCILGG KSRGILRDF KMTRYNFRMN ALMGNIPGVK KASW

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Macromolecule #15: Ribosomal protein S15

MacromoleculeName: Ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 35.803988 KDa
SequenceString: MPPRLPGPQG LRSLTLCLRP AVASPAQALQ PLIQTANISQ KEKKRKMKQD PYGWAQAQQR KAVNVKRQAE LQAQRDAAWG DPVKGITTP FVESFDSAGQ ASVSPPKVGP DGQLVEEPKP LPTSPHLRNY LLNKDEFDSA IQYAEHILKP IKAEDRLTAD P EKEDEEAR ...String:
MPPRLPGPQG LRSLTLCLRP AVASPAQALQ PLIQTANISQ KEKKRKMKQD PYGWAQAQQR KAVNVKRQAE LQAQRDAAWG DPVKGITTP FVESFDSAGQ ASVSPPKVGP DGQLVEEPKP LPTSPHLRNY LLNKDEFDSA IQYAEHILKP IKAEDRLTAD P EKEDEEAR EHAARHAKAV AALERIAKLE HGGAKDRKHA NIRRCIETFG RHITDQSLER PTPPLARGVE PKPQPVRAGP DT GSSEVQI AILTSKIRAL SKALEGHGGN RDKNNKRSLR RLCHKRQRLL RYMERKERGS GRWHHMLETL GLTPATWKGQ ITL

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Macromolecule #16: Ribosomal protein S16, mitochondrial

MacromoleculeName: Ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 12.140166 KDa
SequenceString:
MVLKLRLARF GRTNAPFYNI VVAHARTARN SKPLEVIGTY DPVPKKDTYD TTGKLHKDIK LDVTRAKYWI GVGAQPTDTV WRLLSLVGI LDPKYKKPQP AQEQKTKA

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Macromolecule #17: Mitochondrial 37S ribosomal protein S17

MacromoleculeName: Mitochondrial 37S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 18.505701 KDa
SequenceString:
MASTIAAATK VSRKMFRELH GVVVTSGLID KTVKVRVGGQ KFNKFLQKHF DDPKQYLVHD PNNSLRAGDV VAIMPGFITS KSKRHVVKH IIAPAGTPIE ERPPIPSLDE LWDAKDAAKA AKKERKVLRE KMQAAEEAIE LAERMARHAV REIAMREKII S LQKVD

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Macromolecule #18: Mito ribosomal protein S18

MacromoleculeName: Mito ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 28.468594 KDa
SequenceString: MSSSRQFLSS ALRQCRAAGQ RTAQFSTTSP AAAIRDIPTT SFQNAPRTNT NTSSPSSNNN NNAGSSQYKS DMFNLLNSGA GSRGGRDSY NNARDVDGGQ RYGSNSQKVQ ELLQSEVTSN NYMRMMRRRW NNGDVYAPRD LSPYEMKGRN GGWVEAADVV D ELGFSPLD ...String:
MSSSRQFLSS ALRQCRAAGQ RTAQFSTTSP AAAIRDIPTT SFQNAPRTNT NTSSPSSNNN NNAGSSQYKS DMFNLLNSGA GSRGGRDSY NNARDVDGGQ RYGSNSQKVQ ELLQSEVTSN NYMRMMRRRW NNGDVYAPRD LSPYEMKGRN GGWVEAADVV D ELGFSPLD NYRNFSLISD YITPFGRIRH SKETGLKPVN QRKIAKMVRR AIGLGIHPSV HKHPEILKNS NGRHLLPLVV PK GQKANKT KNFDGEDEN

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Macromolecule #19: Mitochondrial ribosomal protein S19

MacromoleculeName: Mitochondrial ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 10.408332 KDa
SequenceString:
MQPTRVLFKR SVWKGPHIVP LPIQRPEPGK KIAPIRTQAR SATILPNFVG LKFQVHNGKD YIDLTVTEEM VGHKLGEFSA TRKPFIWGK KK

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Macromolecule #20: Mito ribosomal protein S21

MacromoleculeName: Mito ribosomal protein S21 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 25.679785 KDa
SequenceString: MELRSALQSV CRTTAAAASA SARSSLAGRH AFSTSARFQL QPPPNPYVSG KSRIETLRAA LSNRKPAAGA AAGGSPGATS ASSALPPRP PTANDSPWSI VDAINKDSNS STSSSSSSSS SGGALYSSSK PSPMQTWNET DFETRHMAPQ QELNIRLRPS T GRTFYVSG ...String:
MELRSALQSV CRTTAAAASA SARSSLAGRH AFSTSARFQL QPPPNPYVSG KSRIETLRAA LSNRKPAAGA AAGGSPGATS ASSALPPRP PTANDSPWSI VDAINKDSNS STSSSSSSSS SGGALYSSSK PSPMQTWNET DFETRHMAPQ QELNIRLRPS T GRTFYVSG HQDFAGALKL LHRTVAANKV KKDVRLQRFH ERPALKRKRN LRERWRARFK EGFKAAVNRT FELKNQGW

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Macromolecule #21: 37S ribosomal protein S25, mitochondrial

MacromoleculeName: 37S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 29.553588 KDa
SequenceString: MGKGRIFQAA KVYQRASEAA ATNIVSGLPP RNPPLWLKAI ESIPPAEINT RPYPIQHSPP NPRARKPRNL FRPTKIVYPE DELRRDFYR DHPWELARPR MIIELDGKDA RFLDWSKGLR QRGIPLSGER HGNWMLTRIA NDSVVQRQLW LMENQGMTKQ E AYDKARHE ...String:
MGKGRIFQAA KVYQRASEAA ATNIVSGLPP RNPPLWLKAI ESIPPAEINT RPYPIQHSPP NPRARKPRNL FRPTKIVYPE DELRRDFYR DHPWELARPR MIIELDGKDA RFLDWSKGLR QRGIPLSGER HGNWMLTRIA NDSVVQRQLW LMENQGMTKQ E AYDKARHE FYKLRQLEQM ERRIAVEEAR MVGGYFGKDL LTVGMELENK TYESWKKWAT TEIARQESAR ASMYTNVVDN SA LEESEED ELLAQN

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Macromolecule #22: mS26

MacromoleculeName: mS26 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 35.312184 KDa
SequenceString: MAPTLARPSL SGVQFILSSP TTTCAATSVV TRAIAARSFS TTRSARDSVS IPPDSPNYIK VPEPPQSSEV RHPFVKGHLP IPRSIFPKK GVPEKVQSGY VNRIAPKSAA ELAGLPPKSK QESWRRKMAE ARRQSLEAGL QGLWQRKVKR DQKQAKESKA R YLANKRAA ...String:
MAPTLARPSL SGVQFILSSP TTTCAATSVV TRAIAARSFS TTRSARDSVS IPPDSPNYIK VPEPPQSSEV RHPFVKGHLP IPRSIFPKK GVPEKVQSGY VNRIAPKSAA ELAGLPPKSK QESWRRKMAE ARRQSLEAGL QGLWQRKVKR DQKQAKESKA R YLANKRAA QAPERLDEVF TRATIRESTA KNTFVPLDPE AFVKAEEARI KHAEKEAMKS EARRDAVVQL YVASKNFIVD EK ELEEHVN KHFTEKIHNA GLWESGRSIW DSQKNPISMR ELRNEFSGFN DRVTATTSAA VKTTVRQKNV AEELTGGKL

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Macromolecule #23: mS27

MacromoleculeName: mS27 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 44.923723 KDa
SequenceString: MAGRAPQHAL RVGCRAVPEA LSKPAQQSRC LSSTVPRQAT YPVVSFNKTS SPELKEALET LREKVILPTY LPPELRQKIF NKKYEKELA HDPVTIQIDG QPQRFSYINM LTDMPNTPKN IRAALLSMKN GGDFANLSGL LEGMHRANRK LPYWLSAQIV R KACKAGHL ...String:
MAGRAPQHAL RVGCRAVPEA LSKPAQQSRC LSSTVPRQAT YPVVSFNKTS SPELKEALET LREKVILPTY LPPELRQKIF NKKYEKELA HDPVTIQIDG QPQRFSYINM LTDMPNTPKN IRAALLSMKN GGDFANLSGL LEGMHRANRK LPYWLSAQIV R KACKAGHL QLILNMVRDV KRTGFTLERH ETVNELLFWI QRFAWKSDYS EPETRKALRE VQEILDALEG DERHMSKDRK RQ QALTRFP YHRDPQFLAA RLNLTAELAA RRAVTGQTSE QQLNSANDVK NLVKYAEQLV RLWPADKALL DMYTDEAYVA RVD LRYLIK PQVHLRYASF TLQALKNAAK IVGQLGHGPL AAQLINRAAA VEAESQLAYA KVDDGMAGQK IYEMVVGGKK

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Macromolecule #24: Mitochondrial ribosomal protein DAP3

MacromoleculeName: Mitochondrial ribosomal protein DAP3 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 51.451414 KDa
SequenceString: MSASNCLRCL VRPSAVALVP RQMLQVGGGS LTFTATAATK GASSANTGGR QNANRPQKLR FKKTKKKNVV SSGKPPLPGE RKAYRKKIV LSNNNALPVP GLETLRPNDL AKQDNVGSVK ALPEDVVDAL RAMEAFKPTQ CWGIFRQPSV LIRQETVDLT K KMKAAGAD ...String:
MSASNCLRCL VRPSAVALVP RQMLQVGGGS LTFTATAATK GASSANTGGR QNANRPQKLR FKKTKKKNVV SSGKPPLPGE RKAYRKKIV LSNNNALPVP GLETLRPNDL AKQDNVGSVK ALPEDVVDAL RAMEAFKPTQ CWGIFRQPSV LIRQETVDLT K KMKAAGAD GKTIRMVIEG NRVTGKSLLL LQAMTHAFMN DWVVLHIPEA QELTTAVTEY APIENSPLWT QPTYTLKLLQ SF KRANEKV LSRMNTVYSH ADLPQIIPVN SPLLQLINSA KEADGAWTVF QALWRELNAE NVPGRPPILF SLDGLAHIMK VSD YRNPAF ELIHSHDLAL VKLFTDCLSG ATVMPNGGAV LGATTRGNSP RSASMELAIA QREAEKAGEK EVPQRDPYSK KYDD RVEAV MKSVEILRLK GVSKTEARGL LEYWAASGML KKRVDESMVS EKWTLSGNGV VGEMERASLL TMKA

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Macromolecule #25: mS33

MacromoleculeName: mS33 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 12.169154 KDa
SequenceString:
MSVPRARLLQ LVKARCELFS TTFNPEGIRT GNKILRQRLK GPALATYYPR KNVGIRELQK EFGTLGLEVD DEVDDDRLEH LAALRARDK GAPKKKRTAP SAADAKKKK

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Macromolecule #26: 37S ribosomal protein S24, mitochondrial

MacromoleculeName: 37S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 44.05709 KDa
SequenceString: MASSANALRL GLRTCSRPTQ TSNLRIAALQ RRALSATATR CEGVPVPKRE DDAKLEFTRP YNPGEFLSEK LRSDDLQDWE RERYERALT SWEQTPDDLK RGWSTMIRDI EQAAAPLRRV VMPRRSTFWY EEEKDTDLIT NEDGEDDFHE NDIMSLGHGK L EEHREFRE ...String:
MASSANALRL GLRTCSRPTQ TSNLRIAALQ RRALSATATR CEGVPVPKRE DDAKLEFTRP YNPGEFLSEK LRSDDLQDWE RERYERALT SWEQTPDDLK RGWSTMIRDI EQAAAPLRRV VMPRRSTFWY EEEKDTDLIT NEDGEDDFHE NDIMSLGHGK L EEHREFRE YARIAVWEMP LLSKYAKPFV PPTSEEVLRF RYTTYMGEFH PADRKVVVEF CPKDLRDLSE VQQRKLMKLA GP RYNPEKD IIKMSCEKFE HQAQNKRYLG DLIEKMIAAA KDPKDTFEDI PLDTRHHTFT KKISFPKEWL LTEERKKELE AAR QQALLK DAEKVVQGAL VDGADVVKQY LESGAAEALH AVPVMAGRGG KALPGGKGGK MQRSKR

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Macromolecule #27: 37S ribosomal protein mrp10, mitochondrial

MacromoleculeName: 37S ribosomal protein mrp10, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 9.907534 KDa
SequenceString:
MPNKPIRLPP LKQLRVRQAN KAEENPCIAV MSSVLACWAS AGYNSAGCAT VENALRACMD APKPAPKPNN TINYHLSRFQ ERLTQGKSK K

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Macromolecule #28: DUF1713 domain-containing protein

MacromoleculeName: DUF1713 domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 38.12625 KDa
SequenceString: MIPQSVRRVV AAAPQSPVVS SLAASSAPRA GASYILSSYQ PNALQKRRYS SSKPSSPDDG SSRAFAARAS VPAAGTSKTP GEKRKRKAK EPVMPPLPSV PSTRHIKDEA LALSTFFALH RPISVTQLLP KTVTEESFAE IFNHRKGHKA TDVLSTLSQA V HDLESPMS ...String:
MIPQSVRRVV AAAPQSPVVS SLAASSAPRA GASYILSSYQ PNALQKRRYS SSKPSSPDDG SSRAFAARAS VPAAGTSKTP GEKRKRKAK EPVMPPLPSV PSTRHIKDEA LALSTFFALH RPISVTQLLP KTVTEESFAE IFNHRKGHKA TDVLSTLSQA V HDLESPMS GLRLSDNDVE DGSTKISLKH PDGTESDVYF QLNSMSGHFL PFNPPPPPEP IAEVDEAAAE AEASAAIAEE IA ATAEQEP ETRVYKAVVT IEETRDTNGQ YKIMAHSPQL VEDDAVQPRS FLERLALRQI RYEEARQQQG IMHAISVRRQ KKL KIKKKK YKKLMRRTRN ERRKQDRL

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Macromolecule #29: IGR domain-containing protein

MacromoleculeName: IGR domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 26.612898 KDa
SequenceString: MKSSLRIQST LGLLFRPCAV SAPATAPGLN AHARWAHKTA AQLPLIPKPT PFVPDVPTFL TLIGRDLKQH ADKFPTWEAL FTLTTDQLR ELGVEPPRAR RYLLRWRQRF REGKFGIGGD LKHVENGVAY LKIHEKEASP TRTSRRVVNV PANQHVEEVS E GERVKVKG ...String:
MKSSLRIQST LGLLFRPCAV SAPATAPGLN AHARWAHKTA AQLPLIPKPT PFVPDVPTFL TLIGRDLKQH ADKFPTWEAL FTLTTDQLR ELGVEPPRAR RYLLRWRQRF REGKFGIGGD LKHVENGVAY LKIHEKEASP TRTSRRVVNV PANQHVEEVS E GERVKVKG YKVKGVSTIV GPYALPVQKG VAKLAVTEGM WEDKRGHKVD GGERRRAEVR FKRGVAERKA LREKMGFY

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Macromolecule #30: Fe superoxide dismutase

MacromoleculeName: Fe superoxide dismutase / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 34.270785 KDa
SequenceString: MIGSMGLRIP RATSSLLGRA VNAVATNQSA VAGASIAAQR RWQHYLMPLR DNFEQEGIRN FLSPGSVNMA YTEYQTFILE KLNALVVGT DFEQKDTKSI VLATARDPEL AHVFNHASMA HNNHFFFDHL SPVPVKMGDK LFYHINENFG SVDTLRDEMI G TAVSMFGP ...String:
MIGSMGLRIP RATSSLLGRA VNAVATNQSA VAGASIAAQR RWQHYLMPLR DNFEQEGIRN FLSPGSVNMA YTEYQTFILE KLNALVVGT DFEQKDTKSI VLATARDPEL AHVFNHASMA HNNHFFFDHL SPVPVKMGDK LFYHINENFG SVDTLRDEMI G TAVSMFGP GFVWLVRTQL PGQPVALRVM ATYLAGSPYP GAHWRRQEMD AQTSIGSSPQ GLSNGQRFFE RSAAGFKGNK LE PTAPGGT DLIPILCLNT WEYAWLREYG TGVGGMGGKL AYAQSWWNMI DWAKVEEEAR LETRILTGGD SSV

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Macromolecule #31: mS42

MacromoleculeName: mS42 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 39.274625 KDa
SequenceString: MPPRIPNAPS SLLLQSSACS GSSSRCTASR LPTWASSCSS SSPQSSQPTT HQQQCSSFST TAPAHVQSVR RQKMFSWLDK KGSAYKEHT RQGPNLLGGQ GKDGLAVPFP NNPYFKSQPV LSEGSREIIY QDVMEKGLPI KAVSAKYNVD VRRVAAVIRL K EIEKRWIK ...String:
MPPRIPNAPS SLLLQSSACS GSSSRCTASR LPTWASSCSS SSPQSSQPTT HQQQCSSFST TAPAHVQSVR RQKMFSWLDK KGSAYKEHT RQGPNLLGGQ GKDGLAVPFP NNPYFKSQPV LSEGSREIIY QDVMEKGLPI KAVSAKYNVD VRRVAAVIRL K EIEKRWIK EYKPLARPYA RAVMKMLPQT VLGGPDQKPH ESINDVHVHS YTTQQLFVPV SESREFTRED AAKAFGDHIL PV DKKLRVP ELIEFQKDLL KEVPLQEANR KFLNATAASE AKIAEREAKR RQAVEDAITR VKTDRFEFRF QEFNAENVGH DGR DRNAVG WRYGVPFPDR KRSQIKIPTK VE

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Macromolecule #32: mS46

MacromoleculeName: mS46 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 43.112672 KDa
SequenceString: MNRQVVTSTL GRRGVASTIL NAQQQQRPFS STTTRCAAED DSKKPAAAPS TPRAAAPGPI SASRQKSEAA VGKLTQLRGS FTSLTNDNS FHKTLPAGAR DARRLAAAPI AGKGAGAGAV APLGGGGGAS GAPKVINVRS LKGTLGSRGS NNIPGAVAPG A ALRPRFAA ...String:
MNRQVVTSTL GRRGVASTIL NAQQQQRPFS STTTRCAAED DSKKPAAAPS TPRAAAPGPI SASRQKSEAA VGKLTQLRGS FTSLTNDNS FHKTLPAGAR DARRLAAAPI AGKGAGAGAV APLGGGGGAS GAPKVINVRS LKGTLGSRGS NNIPGAVAPG A ALRPRFAA GPGAAAGRPR FGAAASPGAG PTGAARRPPF GARRARPAGD KKRSGGSGDK RPRGDDYDAP PTEEEKAFLR GL EQGKVTE YVPKLTPDTL LGYGPPVATD AALGKVESAM RTMRILGGGL PFNDQSGVTS DPTAIKHRYV HEKKPVFFSS VEE KEWVRE SLDKFAVSEG PEKKTKQKIL ETSVLGKYEE PKYVESLTET VKMVEKYQGG TFSYAPSDAD KFNKKLNQLL AAGL PRAAP APAQAQKKA

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Macromolecule #33: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

MacromoleculeName: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: 3-hydroxyisobutyryl-CoA hydrolase
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 56.401191 KDa
SequenceString: MLVSKSIASR AGLASAVASR SCRPSFTAMP LRAKVLGQQA PISTQAAAEL FKPVEGDEPE DVLFNSLYNL RSVELNRPAK YNALNGSMI RKIAPRLLEW ERSDMANVIV IKGSGEKAFC AGGDVAALAK QNAEGPEGVK KSVDYFGLEY KLNHLISTYT R PYVAFLDG ...String:
MLVSKSIASR AGLASAVASR SCRPSFTAMP LRAKVLGQQA PISTQAAAEL FKPVEGDEPE DVLFNSLYNL RSVELNRPAK YNALNGSMI RKIAPRLLEW ERSDMANVIV IKGSGEKAFC AGGDVAALAK QNAEGPEGVK KSVDYFGLEY KLNHLISTYT R PYVAFLDG ITMGGGVGLS IHAPFRIATE RTVFAMPETK IGFFPDVGAS FFLPRMPGQV GPYLGLTSAL LKGVQVYYAG IA THYLHSS SLPALESRLA ELTPRDYWTI EQRLSVINDT IEEFSTGVPY DENIEIGGKI RLAIDRCFKY DKIDEIIAAL KEE AAEGAK GGVQSWAKNT LEELTQRSPT SLHVTLRQMR LGKSWGIAHT FKREHQMAAK FMKSHDFNEG VTALLIDKGA NGPA KWKPA SLDEIPPGAN ISEDYFRNDP EVPVLELLND RSYMQYPYNK FGLPNDYDVK EAIEKGNFTR EKLIDHFVET RRGKQ GVRE AVSDVLDRMA VRSKGTEHVQ WKKE

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Macromolecule #34: mS47

MacromoleculeName: mS47 / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 10.664098 KDa
SequenceString:
MLRTTVSKLA RPTVSRAFAT TSRALAGETG APPKTGGPGD AFQRREKANE DFAIRQREKE KLLELKKKLA EQQKHLKTLS DHIDEITRE QGGERN

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Macromolecule #36: IF1

MacromoleculeName: IF1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 23.766729 KDa
SequenceString: MSSKLPTTLL RRPSALPSTT TYTAYSASRP TPPSCTAHGA QGQQIRNATF VPRHRRPYQF TQLVQLSDGS TFTVRTTMPT ALYKSAKDS RNHLLWQPSD KSLKNVELDE AGKLAAFRER YGRGWDLDAK MTPEEEAAAA AALAAGGGAG VPGGKAAKKA A EEALLAKK ...String:
MSSKLPTTLL RRPSALPSTT TYTAYSASRP TPPSCTAHGA QGQQIRNATF VPRHRRPYQF TQLVQLSDGS TFTVRTTMPT ALYKSAKDS RNHLLWQPSD KSLKNVELDE AGKLAAFRER YGRGWDLDAK MTPEEEAAAA AALAAGGGAG VPGGKAAKKA A EEALLAKK KKEEEEAAKK AAEAEEADPF DSLTDLISGY ATENMNPGLN FKETRHYGKK K

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Macromolecule #35: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 600.339375 KDa
SequenceString: GAUGUAAUAA AAAAAAUUUU UUUUAAUUUU AUAUUACAUC AAUAAAAAUA GAUGAGUUUG GUGAUGGCUC UGAUUGAACA CUGUCCAAA UACUUGACAC AUGCUAAUCG AACGUUUAAU UUUGGCCUAA GAAAGGGGUU UCAUCGUGGC UUAAGCUAAG G GGUUUAUU ...String:
GAUGUAAUAA AAAAAAUUUU UUUUAAUUUU AUAUUACAUC AAUAAAAAUA GAUGAGUUUG GUGAUGGCUC UGAUUGAACA CUGUCCAAA UACUUGACAC AUGCUAAUCG AACGUUUAAU UUUGGCCUAA GAAAGGGGUU UCAUCGUGGC UUAAGCUAAG G GGUUUAUU GUGGCUUAAG CUAAGGUUUA AUCUUUGACU UAAGCGGGUG UUUUAGGGGA ACUUGUGCCC CUAAAACCUC UU AAUUAAA AGUGGUGUAC AGGUGAGUAU AAUAUUUUUU CGCUUAACUU AAAGUGAAGG CAAAUCCUUC AUAUUGCAAA AGG AUAUCU UAGGCACCUG UUGAAAGGGG CCUACUUAUA UUAUAUCCGC UUUAAGAGGA UGAGAAAAGU UUCAGAGAUA GGUA GUUGU UAAGGUCAUG GCUUAACAAG CCAAUAAUUC UCUUAGUCGA AGCUGAAAAG GCUGAUCGAC CACAUUGGGA AUGAA AAAA UCCCAAGGCA AAUAGGUACA GCAGUGAGGA AUCUUGGUCA AUGGGCCCAC GCCUGAACUG GUAACUUGGA GGAAUG AGG GGUCAACUUU GCAAAUGGAU GAGUGAUCGU UAGAAGAUCC UUAGUCCCCU GGUCUUCUUG ACACAUGAGG UAUAUAC UU CUAGUCCAUA UUGGGGGGAG ACUCCACGUC GAUUUAUCGA GUAAAAUUCU GUAUACAUAU UGAUAAUGAC AAUAUGUA C AUUUGUCUUG ACUAAUUACG UGCCAGCAGU CGCGGCAAUA CGUAAGAGAC UAGUGUUAAU CAUCAUAAAU AGGUUUAAA GGGUACUCAG ACGGAAAAAU UCGCCCAAAU AUAGGGGACA AUUUUUCUAG AGUUUUAUGU AAGAAGGUCG UACUCUAGAG UGGAGAGAU AAAAUUCUGU GAUACCUAGG GGACGGGUAA AGGCGAAGGC AAUCUUUUAU GUAAAAACUG ACGUCGAAGG A CGAAGGCA AAGGGAACAA AAAGGAUUAG AUACCCCAGU AGUCUUUGCA GACAAUUAUG AAUGCCAUAG GUUAGAUUUU UA AUUUAGU CUAUAAAUGA AAGUGUAAGC AUUUCACCUC AAGAGUAAGG CGGCAACGCA GGAACUGAAA UCACUAGACC GUU UCUGAC ACCAGCAAUG AAGUAUGUUA UUUAAUUCGG UGACCCACGA AAAACCUUAC CACAAUUUGA AUAUUAAUAA UAAU GAUAU UAUUUUUUAU GCUUGAUAUG GCAAGCACUC AAUUUUCCCC UCCCCGUAGG UUUGCCGCGG GGGGGGAGAA AAAAG AAAA AUAAUGGAUA AUAUAGUAAA UACCAUAUUC CAACUAUAUU UAAUUAUUAA UACAAGUGUU GCACGGCUGU CUUCAG UUG AUGUUGCGAA ACUGUGGUUC GUUCCAUGGA AUUAACGUAA ACCCUUGCUU UAUUUGUAAA UAUUAUAAAG CAGUUCA CC UUUAUAUAGG AAAUGAUAAA AGGGAUCAAG ACAAGUCAUC AUGGCCUAAA UAUUGUGGGC UAUAGACGUG CCACAUUU U CCUAAACAAA GAGAUGCAAA AAUGUGAAUU UUAGCUAAUC UCAAAAAAUA GGAUAAAAAU AUACAAGGAU UGUAGUCUG AAAUUCGACU GCAUGAAUAA GAAAUUGCUA GUAAUCGUGA AUCACCAUGA CACGGUGAAU AUUCCCUCGG AUUGGUACUA ACCACUCGU CACAUGCUGA AAGGAGUGCG UGCAAUAAGU UUGCUUUUCU GUUAUAAGUA AGUAGACAUA UAGGUUUAGA U GUUAUAAU AGGAUCCUUC GUAUGCGCGG CUCUGAUUAG UGUUAAGUCG AAAUACGGUU CGUGUAGUGG AAGUUGCACG GG ACUUAUC AAUGUUGAAC AAUACGA

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Macromolecule #37: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 97 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #38: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Macromolecule #39: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 39 / Number of copies: 22 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMHEPES-KOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-KOH
15.0 mMKClPotassium chloride
15.0 mMMg(C2H3O2)2Magnesium acetate
0.05 %DDMn-Dodecyl B-D-maltoside
0.0075 %Cardiolipin1,3-bis(sn-3-phosphatidyl)-sn-glycerol
2.0 mMDTTDithiothreitol
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.7 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-20 / Number real images: 3172 / Average electron dose: 35.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf
Startup modelType of model: EMDB MAP
EMDB ID:

3551

Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 131806
FSC plot (resolution estimation)

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