+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-10262 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Yeast 80S ribosome stalled on SDD1 mRNA. | |||||||||
マップデータ | SDD1 stalled 80S yeast ribosome | |||||||||
試料 |
| |||||||||
機能・相同性 | 機能・相同性情報 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / Protein methylation / : / positive regulation of translational fidelity / RMTs methylate histone arginines / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process ...maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / Protein methylation / : / positive regulation of translational fidelity / RMTs methylate histone arginines / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / ヒドロキシル化 / GDP-dissociation inhibitor activity / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of mRNA splicing, via spliceosome / ribosomal small subunit export from nucleus / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / 90S preribosome / protein-RNA complex assembly / ribosomal large subunit export from nucleus / Ub-specific processing proteases / G-protein alpha-subunit binding / regulation of translational fidelity / positive regulation of protein kinase activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal subunit export from nucleus / translational termination / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / ribosomal large subunit biogenesis / DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of LSU-rRNA / cellular response to amino acid starvation / ribosome assembly / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / オートファジー / modification-dependent protein catabolic process / ribosomal large subunit assembly / rRNA processing / ribosomal small subunit biogenesis / protein tag activity / small ribosomal subunit rRNA binding / cytoplasmic stress granule / ribosomal small subunit assembly / ribosome binding / リボソーム生合成 / large ribosomal subunit rRNA binding / small ribosomal subunit / cytosolic small ribosomal subunit / 5S rRNA binding / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / protein ubiquitination / リボソーム / structural constituent of ribosome / positive regulation of protein phosphorylation / 翻訳 (生物学) / G protein-coupled receptor signaling pathway / response to antibiotic / negative regulation of gene expression / mRNA binding / ubiquitin protein ligase binding / 核小体 / ミトコンドリア / RNA binding / zinc ion binding / 核質 / metal ion binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | |||||||||
生物種 | Saccharomyces cerevisiae (パン酵母) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.8 Å | |||||||||
データ登録者 | Tesina P / Buschauer R / Cheng J / Becker T / Beckmann R | |||||||||
資金援助 | ドイツ, 1件
| |||||||||
引用 | ジャーナル: Nat Struct Mol Biol / 年: 2020 タイトル: RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1. 著者: Yoshitaka Matsuo / Petr Tesina / Shizuka Nakajima / Masato Mizuno / Akinori Endo / Robert Buschauer / Jingdong Cheng / Okuto Shounai / Ken Ikeuchi / Yasushi Saeki / Thomas Becker / Roland ...著者: Yoshitaka Matsuo / Petr Tesina / Shizuka Nakajima / Masato Mizuno / Akinori Endo / Robert Buschauer / Jingdong Cheng / Okuto Shounai / Ken Ikeuchi / Yasushi Saeki / Thomas Becker / Roland Beckmann / Toshifumi Inada / 要旨: Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation ...Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the ubiquitin-dependent ribosome dissociation remain poorly understood. Here, we identified SDD1 messenger RNA from Saccharomyces cerevisiae as an endogenous RQC substrate and reveal the mechanism of its mRNA-dependent and nascent peptide-dependent translational stalling. In vitro translation of SDD1 mRNA enabled the reconstitution of Hel2-dependent polyubiquitination of collided disomes and, preferentially, trisomes. The distinct trisome architecture, visualized using cryo-EM, provides the structural basis for the more-efficient recognition by Hel2 compared with that of disomes. Subsequently, the Slh1 helicase subunit of the RQC trigger (RQT) complex preferentially dissociates the first stalled polyubiquitinated ribosome in an ATP-dependent manner. Together, these findings provide fundamental mechanistic insights into RQC and its physiological role in maintaining cellular protein homeostasis. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_10262.map.gz | 141.6 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-10262-v30.xml emd-10262.xml | 87.6 KB 87.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_10262_fsc.xml | 14.2 KB | 表示 | FSCデータファイル |
画像 | emd_10262.png | 90.6 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-10262 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10262 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|---|
「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_10262.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | SDD1 stalled 80S yeast ribosome | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.084 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
+全体 : Yeast 80S ribosome stalled on SDD1 mRNA.
+超分子 #1: Yeast 80S ribosome stalled on SDD1 mRNA.
+分子 #1: Saccharomyces cerevisiae S288C 18S ribosomal RNA (RDN18-1), rRNA
+分子 #35: 5S rRNA
+分子 #36: 5.8S rRNA
+分子 #78: Saccharomyces cerevisiae S288C 25S ribosomal RNA (RDN25-1), rRNA
+分子 #79: mRNA
+分子 #81: tRNA
+分子 #2: 40S ribosomal protein S0-A
+分子 #3: 40S ribosomal protein S1-A
+分子 #4: 40S ribosomal protein S15
+分子 #5: 40S ribosomal protein S2
+分子 #6: 40S ribosomal protein S3
+分子 #7: 40S ribosomal protein S4-A
+分子 #8: 40S ribosomal protein S5
+分子 #9: 40S ribosomal protein S6-A
+分子 #10: 40S ribosomal protein S7-A
+分子 #11: 40S ribosomal protein S8-A
+分子 #12: 40S ribosomal protein S9-A
+分子 #13: 40S ribosomal protein S10-A
+分子 #14: 40S ribosomal protein S11-A
+分子 #15: 40S ribosomal protein S12
+分子 #16: 40S ribosomal protein S13
+分子 #17: 40S ribosomal protein S14-A
+分子 #18: 40S ribosomal protein S16-A
+分子 #19: 40S ribosomal protein S17-B
+分子 #20: 40S ribosomal protein S18-A
+分子 #21: 40S ribosomal protein S19-A
+分子 #22: 40S ribosomal protein S20
+分子 #23: 40S ribosomal protein S21-A
+分子 #24: 40S ribosomal protein S22-A
+分子 #25: 40S ribosomal protein S23-A
+分子 #26: 40S ribosomal protein S24-A
+分子 #27: 40S ribosomal protein S25-A
+分子 #28: 40S ribosomal protein S26-A
+分子 #29: 40S ribosomal protein S27-A
+分子 #30: 40S ribosomal protein S29-A
+分子 #31: 40S ribosomal protein S30-A
+分子 #32: Ubiquitin-40S ribosomal protein S31
+分子 #33: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #34: 40S ribosomal protein S28-B
+分子 #37: 60S ribosomal protein L2-A
+分子 #38: 60S ribosomal protein L3
+分子 #39: 60S ribosomal protein L4-A
+分子 #40: 60S ribosomal protein L5
+分子 #41: 60S ribosomal protein L6-B
+分子 #42: 60S ribosomal protein L7-A
+分子 #43: 60S ribosomal protein L8-A
+分子 #44: 60S ribosomal protein L9-A
+分子 #45: 60S ribosomal protein L10
+分子 #46: 60S ribosomal protein L11-A
+分子 #47: 60S ribosomal protein L13-A
+分子 #48: 60S ribosomal protein L14-A
+分子 #49: 60S ribosomal protein L15-A
+分子 #50: 60S ribosomal protein L16-A
+分子 #51: 60S ribosomal protein L17-A
+分子 #52: 60S ribosomal protein L18-A
+分子 #53: 60S ribosomal protein L19-A
+分子 #54: 60S ribosomal protein L20-A
+分子 #55: 60S ribosomal protein L21-A
+分子 #56: 60S ribosomal protein L22-A
+分子 #57: 60S ribosomal protein L23-A
+分子 #58: 60S ribosomal protein L24-A
+分子 #59: 60S ribosomal protein L25
+分子 #60: 60S ribosomal protein L26-A
+分子 #61: 60S ribosomal protein L27-A
+分子 #62: 60S ribosomal protein L28
+分子 #63: 60S ribosomal protein L29
+分子 #64: 60S ribosomal protein L30
+分子 #65: 60S ribosomal protein L31-A
+分子 #66: 60S ribosomal protein L32
+分子 #67: 60S ribosomal protein L33-A
+分子 #68: 60S ribosomal protein L34-A
+分子 #69: 60S ribosomal protein L35-A
+分子 #70: 60S ribosomal protein L36-A
+分子 #71: 60S ribosomal protein L37-A
+分子 #72: 60S ribosomal protein L38
+分子 #73: 60S ribosomal protein L39
+分子 #74: Ubiquitin-60S ribosomal protein L40
+分子 #75: L41-A
+分子 #76: 60S ribosomal protein L42-A
+分子 #77: 60S ribosomal protein L43-A
+分子 #80: Uncharacterized protein YEL057C
+分子 #82: MAGNESIUM ION
+分子 #83: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
---|---|
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 2.5 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |