SASDFG5: His-RuvBl1/RuvBl2 dodecamer (RuvB-like 1 + RuvB-like 2)

Basic information

• Entry: Database: SASBDB / ID: SASDFG5

Sample

His-RuvBl1/RuvBl2 dodecamer

• RuvB-like 1 (protein), Homo sapiens
• RuvB-like 2 (protein), Homo sapiens

Function / homology

Function:

promoter-enhancer loop anchoring activity / regulation of DNA strand elongation / positive regulation of telomere maintenance in response to DNA damage / establishment of protein localization to chromatin / R2TP complex / Swr1 complex / dynein axonemal particle / RPAP3/R2TP/prefoldin-like complex / regulation of double-strand break repair / positive regulation of telomerase RNA localization to Cajal body / Ino80 complex / box C/D snoRNP assembly / protein folding chaperone complex / NuA4 histone acetyltransferase complex / regulation of chromosome organization / positive regulation of double-strand break repair via homologous recombination / regulation of DNA replication / MLL1 complex / TFIID-class transcription factor complex binding / regulation of embryonic development / Telomere Extension By Telomerase / RNA polymerase II core promoter sequence-specific DNA binding / regulation of DNA repair / Deposition of new CENPA-containing nucleosomes at the centromere / positive regulation of DNA repair / TBP-class protein binding / DNA helicase activity / telomere maintenance / cellular response to estradiol stimulus / ADP binding / Formation of the beta-catenin:TCF transactivating complex / DNA Damage Recognition in GG-NER / euchromatin / negative regulation of canonical Wnt signaling pathway / chromatin DNA binding / beta-catenin binding / nuclear matrix / positive regulation of canonical Wnt signaling pathway / transcription corepressor activity / UCH proteinases / cellular response to UV / unfolded protein binding / nucleosome / protein folding / HATs acetylate histones / ATPase binding / spermatogenesis / regulation of apoptotic process / DNA recombination / DNA helicase / transcription coactivator activity / protein stabilization / regulation of cell cycle / Ub-specific processing proteases / chromatin remodeling / cadherin binding / cell cycle / ribonucleoprotein complex / RNA polymerase II cis-regulatory region sequence-specific DNA binding / cell division / DNA repair / centrosome / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / ATP hydrolysis activity / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / extracellular exosome / nucleoplasm / ATP binding / membrane / identical protein binding / nucleus / cytosol / cytoplasm

Domain/homology:

RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase

Component:

RuvB-like 2 / RuvB-like 1

• Biological species: Homo sapiens (human)

Contact author

• Raphael Dos Santos Morais (University of Lorraine)

Models

• Model #2934: ; Type: dummy / Radius of dummy atoms: 3.30 A / Chi-square value: 5.098 / P-value: 0.000006

Sample

• Sample: Name: His-RuvBl1/RuvBl2 dodecamer / Specimen concentration: 6 mg/ml / Entity id: 1608 / 1609
• Buffer: Name: HEPES 20 mM, NaCl 150 mM, Glycerol 1%, TCEP 5 mM / pH: 7.5

Entity #1608

Type: protein / Description: RuvB-like 1 / Formula weight: 51.759 / Num. of mol.: 6 / Source: Homo sapiens / References: UniProt: Q9Y265
Sequence:

MVHHHHHHLV PRGSKIEEVK STTKTQRIAS HSHVKGLGLD ESGLAKQAAS GLVGQENARE ACGVIVELIK SKKMAGRAVL LAGPPGTGKT ALALAIAQEL GSKVPFCPMV GSEVYSTEIK KTEVLMENFR RAIGLRIKET KEVYEGEVTE LTPCETENPM GGYGKTISHV IIGLKTAKGT KQLKLDPSIF ESLQKERVEA GDVIYIEANS GAVKRQGRCD TYATEFDLEA EEYVPLPKGD VHKKKEIIQD VTLHDLDVAN ARPQGGQDIL SMMGQLMKPK KTEITDKLRG EINKVVNKYI DQGIAELVPG VLFVDEVHML DIECFTYLHR ALESSIAPIV IFASNRGNCV IRGTEDITSP HGIPLDLLDR VMIIRTMLYT PQEMKQIIKI RAQTEGINIS EEALNHLGEI GTKTTLRYSV QLLTPANLLA KINGKDSIEK EHVEEISELF YDAKSSAKIL ADQQDKYMK

Entity #1609

Type: protein / Description: RuvB-like 2 / Formula weight: 51.886 / Num. of mol.: 6 / Source: Homo sapiens / References: UniProt: Q9Y230
Sequence:

MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL AARRAAGVVL EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF TAIAGSEIFS LEMSKTEALT QAFRRSIGVR IKEETEIIEG EVVEIQIDRP ATGTGSKVGK LTLKTTEMET IYDLGTKMIE SLTKDKVQAG DVITIDKATG KISKLGRSFT RARDYDAMGS QTKFVQCPDG ELQKRKEVVH TVSLHEIDVI NSRTQGFLAL FSGDTGEIKS EVREQINAKV AEWREEGKAE IIPGVLFIDE VHMLDIESFS FLNRALESDM APVLIMATNR GITRIRGTSY QSPHGIPIDL LDRLLIVSTT PYSEKDTKQI LRIRCEEEDV EMSEDAYTVL TRIGLETSLR YAIQLITAAS LVCRKRKGTE VQVDDIKRVY SLFLDESRST QYMKEYQDAF LFNELKGETM DTSLEVLFQ

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 2 mm
• Detector: Name: Eiger 4M / Pixsize x: 75 mm

Scan

Title: His-RuvBl1/RuvBl2 dodecamer / Measurement date: Mar 21, 2019 / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 17 / Unit: 1/A /

	Min	Max
Q	0.0041	0.5786

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 443 /

	Min	Max
Q	0.00729749	0.208798
P(R) point	1	443
R	0	200

Result

Type of curve: sec
Comments: The experimental molecular weight was determined from SEC-RALS/LALS experiment (650 kDa).

	Experimental	Porod
MW	650 kDa	-
Volume	-	1490 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.1974	0.1979	0.0001
Radius of gyration, Rg	6.08 nm	6.15 nm	0.06

	Min	Max
D	-	20
Guinier point	6	38