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TitleStructural mechanisms of Tad pilus assembly and its interaction with an RNA virus.
Journal, issue, pagesSci Adv, Vol. 10, Issue 18, Page eadl4450, Year 2024
Publish dateMay 3, 2024
AuthorsYuhang Wang / Matthew Theodore / Zhongliang Xing / Utkarsh Narsaria / Zihao Yu / Lanying Zeng / Junjie Zhang /
PubMed Abstract Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV ... Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV pilins, Tad pilins lack the typical globular β sheet domain responsible for pilus assembly and phage binding. The mechanisms of Tad pilus assembly and its interaction with phage ΦCb5 have been elusive. Using cryo-electron microscopy, we unveiled the Tad pilus assembly mechanism, featuring a unique network of hydrogen bonds at its core. We then identified the Tad pilus binding to the ΦCb5 maturation protein (Mat) through its β region. Notably, the amino terminus of ΦCb5 Mat is exposed outside the capsid and phage/pilus interface, enabling the attachment of fluorescent and affinity tags. These engineered ΦCb5 virions can be efficiently assembled and purified in , maintaining infectivity against , which presents promising applications, including RNA delivery and phage display.
External linksSci Adv / PubMed:38701202 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution0.86 - 6.45 Å
Structure data

41844

8u2b

EMDB-41844, PDB-8u2b:
Cryo-EM structure of C.crescentus bNY30a pilus complex
Method: EM (helical sym.) / Resolution: 0.86 Å

42136

8ucr

EMDB-42136, PDB-8ucr:
PhiCb5 maturation protein with Caulobacter crescentus bNY30a pili
Method: EM (single particle) / Resolution: 6.45 Å

42163

8uej

EMDB-42163, PDB-8uej:
ssRNA phage PhiCb5 virion
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-CA:
Unknown entry

Source
  • caulobacter vibrioides (bacteria)
  • caulobacter phage phicb5 (virus)
KeywordsPROTEIN FIBRIL / Complex / RECOMBINATION / RNA phage / VIRUS / ssRNA phage

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