Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of bacteriophage T5 infection trigger and cell wall perforation.
Journal, issue, pages	Sci Adv, Vol. 9, Issue 12, Page eade9674, Year 2023
Publish date	Mar 24, 2023
Authors	Romain Linares / Charles-Adrien Arnaud / Grégory Effantin / Claudine Darnault / Nathan Hugo Epalle / Elisabetta Boeri Erba / Guy Schoehn / Cécile Breyton /
PubMed Abstract	Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a ...Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a long flexible tail () at the tip of which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, the unique RBP is located at the extremity of a central fiber. We present the structures of T5 tail tip, determined by cryo-electron microscopy before and after interaction with its receptor, FhuA, reconstituted into nanodisc. These structures bring out the important conformational changes undergone by T5 tail tip upon infection, which include bending of T5 central fiber on the side of the tail tip, tail anchoring to the membrane, tail tube opening, and formation of a transmembrane channel. The data allow to detail the first steps of an otherwise undescribed infection mechanism.
External links	Sci Adv / PubMed:36961893 / PubMed Central
Methods	EM (single particle)
Resolution	3.45 - 4.32 Å
Structure data	13953 7qg9 EMDB-13953, PDB-7qg9: Tail tip of siphophage T5 : common core proteins Method: EM (single particle) / Resolution: 3.45 Å 14733 7zhj EMDB-14733, PDB-7zhj: Tail tip of siphophage T5 : tip proteins Method: EM (single particle) / Resolution: 3.53 Å 14790 7zlv EMDB-14790: Tail tip of siphophage T5 : central fibre PDB-7zlv: Tail tip of siphophage T5 : central fibre protein pb4 Method: EM (single particle) / Resolution: 4.22 Å 14799 7zn2 EMDB-14799, PDB-7zn2: Tail tip of siphophage T5 : full complex after interaction with its bacterial receptor FhuA Method: EM (single particle) / Resolution: 4.29 Å 14800 7zn4 EMDB-14800, PDB-7zn4: Tail tip of siphophage T5 : bent fibre after interaction with its bacterial receptor FhuA Method: EM (single particle) / Resolution: 4.32 Å 14869 7zqb EMDB-14869, PDB-7zqb: Tail tip of siphophage T5 : full structure Method: EM (single particle) / Resolution: 3.88 Å 14873 7zqp EMDB-14873, PDB-7zqp: Tail tip of siphophage T5 : open cone after interaction with bacterial receptor FhuA Method: EM (single particle) / Resolution: 3.6 Å
Source	Enterobacteria phage T5 (virus) escherichia phage t5 (virus)
Keywords	VIRAL PROTEIN / Bacteriophage / Siphophage / T5 / baseplate / VIRUS LIKE PARTICLE