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TitleCryo-EM captures a unique conformational rearrangement in 23S rRNA helices of the Mycobacterium 50S subunit.
Journal, issue, pagesInt J Biol Macromol, Vol. 253, Issue Pt 3, Page 126876, Year 2023
Publish dateDec 31, 2023
AuthorsPriya Baid / Jayati Sengupta /
PubMed AbstractStructural investigations of the ribosomes isolated from pathogenic and non-pathogenic Mycobacterium species have identified several mycobacteria-specific structural features of ribosomal RNA and ...Structural investigations of the ribosomes isolated from pathogenic and non-pathogenic Mycobacterium species have identified several mycobacteria-specific structural features of ribosomal RNA and proteins. Here, we report structural evidence of a hitherto unknown conformational switch of mycobacterium 23S rRNA helices (H54a and H67-H71). Cryo-electron microscopy (cryo-EM) structures (~3-4 Å) of the M. smegmatis (Msm) log-phase 50S ribosomal subunit revealed conformational variability in H67-H71 region of the 23S rRNA, and manifested that, while H68 possesses the usual stretched conformation in one class of the maps, another one exhibits a bulge-out, fused density of H68-H69 at the inter-subunit surface, indicating an intrinsic dynamics of these rRNA helices. Remarkably, altered conformation of H68 forming a more prominent bulge-out structure at the inter-subunit surface of the 50S subunit due to the conformational rearrangements of 23S rRNA H67-H71 region was clearly visualized in a 3 Å cryo-EM map of the 50S subunit obtained from the stationary phase ribosome dataset. The Msm50S subunit having such bulge-out conformation at the intersubunit surface would be incompatible for associating with the 30S subunit due to its inability to form major inter-subunit bridges. Evidently, availability of active 70S ribosome pool can be modulated by stabilizing either one of the H68 conformation.
External linksInt J Biol Macromol / PubMed:37709237
MethodsEM (single particle)
Resolution3.5 - 4.1 Å
Structure data

33096

7xam

EMDB-33096, PDB-7xam:
Mycobacterium smegmatis 50S ribosomal subunit from Stationary phase of growth
Method: EM (single particle) / Resolution: 3.5 Å

33599

7y41

EMDB-33599, PDB-7y41:
Mycobacterium smegmatis 50S ribosomal subunit from Log Phase of growth
Method: EM (single particle) / Resolution: 4.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsRIBOSOME / 50S Subunit / Domain IV of 23S rRNA / Alternate conformation / Helix 68 / Mycobacterium 50S subunit / Log Phase

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