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TitleThree structures of PSI-LHCI from Chlamydomonas reinhardtii suggest a resting state re-activated by ferredoxin.
Journal, issue, pagesBiochim Biophys Acta Bioenerg, Vol. 1864, Issue 4, Page 148986, Year 2023
Publish dateNov 1, 2023
AuthorsChristoph Gerle / Yuko Misumi / Akihiro Kawamoto / Hideaki Tanaka / Hisako Kubota-Kawai / Ryutaro Tokutsu / Eunchul Kim / Dror Chorev / Kazuhiro Abe / Carol V Robinson / Kaoru Mitsuoka / Jun Minagawa / Genji Kurisu /
PubMed AbstractPhotosystem I (PSI) from the green alga Chlamydomonas reinhardtii, with various numbers of membrane bound antenna complexes (LHCI), has been described in great detail. In contrast, structural ...Photosystem I (PSI) from the green alga Chlamydomonas reinhardtii, with various numbers of membrane bound antenna complexes (LHCI), has been described in great detail. In contrast, structural characterization of soluble binding partners is less advanced. Here, we used X-ray crystallography and single particle cryo-EM to investigate three structures of the PSI-LHCI supercomplex from Chlamydomonas reinhardtii. An X-ray structure demonstrates the absence of six chlorophylls from the luminal side of the LHCI belts, suggesting these pigments were either physically absent or less stably associated with the complex, potentially influencing excitation transfer significantly. CryoEM revealed extra densities on luminal and stromal sides of the supercomplex, situated in the vicinity of the electron transfer sites. These densities disappeared after the binding of oxidized ferredoxin to PSI-LHCI. Based on these structures, we propose the existence of a PSI-LHCI resting state with a reduced active chlorophyll content, electron donors docked in waiting positions and regulatory binding partners positioned at the electron acceptor site. The resting state PSI-LHCI supercomplex would be recruited to its active form by the availability of oxidized ferredoxin.
External linksBiochim Biophys Acta Bioenerg / PubMed:37270022
MethodsEM (single particle) / X-ray diffraction
Resolution3.4 - 4.9 Å
Structure data

32892

7wyi

EMDB-32892, PDB-7wyi:
Native Photosystem I of Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.9 Å

32907

7wzn

EMDB-32907, PDB-7wzn:
PSI-LHCI from Chlamydomonas reinhardtii with bound ferredoxin
Method: EM (single particle) / Resolution: 4.9 Å

PDB-8h2u:
X-ray Structure of photosystem I-LHCI super complex from Chlamydomonas reinhardtii.
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

Chemicals

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPHOTOSYNTHESIS / membrane supercomplex / light harvesting / electron transport / green algae / Photosystem I / light-harvesting chlorophyll protein complex I

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