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TitleCryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB.
Journal, issue, pagesProtein Cell, Vol. 14, Issue 6, Page 448-458, Year 2023
Publish dateJun 7, 2023
AuthorsShan Sun / Yan Gao / Xiaolin Yang / Xiuna Yang / Tianyu Hu / Jingxi Liang / Zhiqi Xiong / Yuting Ran / Pengxuan Ren / Fang Bai / Luke W Guddat / Haitao Yang / Zihe Rao / Bing Zhang /
PubMed AbstractThe adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, IrtAB, plays a vital role in the replication and viability of Mycobacterium tuberculosis (Mtb), where its function is to import ...The adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, IrtAB, plays a vital role in the replication and viability of Mycobacterium tuberculosis (Mtb), where its function is to import iron-loaded siderophores. Unusually, it adopts the canonical type IV exporter fold. Herein, we report the structure of unliganded Mtb IrtAB and its structure in complex with ATP, ADP, or ATP analogue (AMP-PNP) at resolutions ranging from 2.8 to 3.5 Å. The structure of IrtAB bound ATP-Mg2+ shows a "head-to-tail" dimer of nucleotide-binding domains (NBDs), a closed amphipathic cavity within the transmembrane domains (TMDs), and a metal ion liganded to three histidine residues of IrtA in the cavity. Cryo-electron microscopy (Cryo-EM) structures and ATP hydrolysis assays show that the NBD of IrtA has a higher affinity for nucleotides and increased ATPase activity compared with IrtB. Moreover, the metal ion located in the TM region of IrtA is critical for the stabilization of the conformation of IrtAB during the transport cycle. This study provides a structural basis to explain the ATP-driven conformational changes that occur in IrtAB.
External linksProtein Cell / PubMed:36882106 / PubMed Central
MethodsEM (single particle)
Resolution2.88 - 3.53 Å
Structure data

32536

7wiu

EMDB-32536, PDB-7wiu:
Cryo-EM structure of Mycobacterium tuberculosis irtAB in inward-facing state
Method: EM (single particle) / Resolution: 3.48 Å

32537

7wiv

EMDB-32537, PDB-7wiv:
Cryo-EM structure of Mycobacterium tuberculosis irtAB in complex with an AMP-PNP
Method: EM (single particle) / Resolution: 2.88 Å

32538

7wiw

EMDB-32538, PDB-7wiw:
Cryo-EM structure of Mycobacterium tuberculosis irtAB complexed with ATP in an occluded conformation
Method: EM (single particle) / Resolution: 3.12 Å

32539

7wix

EMDB-32539, PDB-7wix:
Cryo-EM structure of Mycobacterium tuberculosis irtAB in complex with ADP
Method: EM (single particle) / Resolution: 3.53 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • mycobacterium tuberculosis h37rv (bacteria)
KeywordsMEMBRANE PROTEIN / IrtAB / ABC exporter-liker importer / Iron-loaded siderophore / Mycobacterium tuberculosis

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