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Structure paper

TitleMolecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 5824, Year 2022
Publish dateOct 3, 2022
AuthorsRyan Puskar / Chloe Du Truong / Kyle Swain / Saborni Chowdhury / Ka-Yi Chan / Shan Li / Kai-Wen Cheng / Ting Yu Wang / Yu-Ping Poh / Yuval Mazor / Haijun Liu / Tsui-Fen Chou / Brent L Nannenga / Po-Lin Chiu /
PubMed AbstractThe photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex ...The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex mediates the transfer of light energy from the chlorosome antenna complex to the RC. Here we determine the structure of the photosynthetic supercomplex from the GSB Chlorobaculum tepidum using single-particle cryogenic electron microscopy (cryo-EM) and identify the cytochrome c subunit (PscC), two accessory protein subunits (PscE and PscF), a second FMO trimeric complex, and a linker pigment between FMO and the RC core. The protein subunits that are assembled with the symmetric RC core generate an asymmetric photosynthetic supercomplex. One linker bacteriochlorophyll (BChl) is located in one of the two FMO-PscA interfaces, leading to differential efficiencies of the two energy transfer branches. The two FMO trimeric complexes establish two different binding interfaces with the RC cytoplasmic surface, driven by the associated accessory subunits. This structure of the GSB photosynthetic supercomplex provides mechanistic insight into the light excitation energy transfer routes and a possible evolutionary transition intermediate of the bacterial photosynthetic supercomplex from the primitive homodimeric RC.
External linksNat Commun / PubMed:36192412 / PubMed Central
MethodsEM (single particle)
Resolution3.08 - 3.49 Å
Structure data

26469

7uea

EMDB-26469, PDB-7uea:
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO1)
Method: EM (single particle) / Resolution: 3.49 Å

26471

7ueb

EMDB-26471, PDB-7ueb:
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO2)
Method: EM (single particle) / Resolution: 3.08 Å

Chemicals

ChemComp-GS0:
Bacteriochlorophyll A isomer

ChemComp-BCL:
BACTERIOCHLOROPHYLL A / Bacteriochlorophyll

ChemComp-F39:
[(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate

ChemComp-F26:
2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-CA:
Unknown entry

ChemComp-G2O:
Chlorophyll A ester

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Source
  • chlorobaculum tepidum tls (bacteria)
KeywordsPHOTOSYNTHESIS / FMO / reaction center / single-particle cryo-EM / bacteriochlorophyll / electron transport chain / energy transfer

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