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TitleHigh-resolution cryo-EM structure of the virus Sf6 genome delivery tail machine.
Journal, issue, pagesSci Adv, Vol. 8, Issue 49, Page eadc9641, Year 2022
Publish dateDec 9, 2022
AuthorsFenglin Li / Chun-Feng David Hou / Ruoyu Yang / Richard Whitehead / Carolyn M Teschke / Gino Cingolani /
PubMed AbstractSf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom ...Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6° with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae.
External linksSci Adv / PubMed:36475795 / PubMed Central
MethodsEM (single particle)
Resolution2.76 - 3.73 Å
Structure data

25101

7sfs

EMDB-25101, PDB-7sfs:
In situ cryo-EM structure of bacteriophage Sf6 portal:gp7 complex at 2.7A resolution
Method: EM (single particle) / Resolution: 2.76 Å

25106

7sg7

EMDB-25106, PDB-7sg7:
In situ cryo-EM structure of bacteriophage Sf6 gp8:gp14N complex at 2.8 A resolution
Method: EM (single particle) / Resolution: 2.83 Å

25365

7sp4

EMDB-25365, PDB-7sp4:
In situ cryo-EM structure of bacteriophage Sf6 gp3:gp7:gp5 complex in conformation 2 at 3.71A resolution
Method: EM (single particle) / Resolution: 3.71 Å

25372

7spu

EMDB-25372, PDB-7spu:
In situ cryo-EM structure of bacteriophage Sf6 gp3:gp7:gp5 complex in conformation 1 at 3.73A resolution
Method: EM (single particle) / Resolution: 3.73 Å

26582

7ukj

EMDB-26582, PDB-7ukj:
In situ cryo-EM structure of bacteriophage Sf6 portal:gp7 complex at 2.7A resolution
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • Shigella flexneri bacteriophage VI, Bacteriophage SfVI
  • shigella phage sf6 (virus)
KeywordsSTRUCTURAL PROTEIN / in situ / phage / portal / gp7 / gp3 / gp8 / gp14 / bacteriophage / Sf6 portal / coat protein / symmetry mismatch / VIRAL PROTEIN

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