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Structure paper

TitleStructure of Photosystem I Supercomplex Isolated from a Cytochrome b6f Temperature-Sensitive Mutant.
Journal, issue, pagesBiomolecules, Vol. 13, Issue 3, Year 2023
Publish dateMar 15, 2023
AuthorsTom Schwartz / Mariia Fadeeva / Daniel Klaiman / Nathan Nelson /
PubMed AbstractThe unicellular green alga, , has been widely used as a model system to study photosynthesis. Its possibility to generate and analyze specific mutants has made it an excellent tool for mechanistic ...The unicellular green alga, , has been widely used as a model system to study photosynthesis. Its possibility to generate and analyze specific mutants has made it an excellent tool for mechanistic and biogenesis studies. Using negative selection of ultraviolet (UV) irradiation-mutated cells, we isolated a mutant (TSP9) with a single amino acid mutation in the Rieske protein of the cytochrome b6f complex. The W143R mutation in the petC gene resulted in total loss of cytochrome b6f complex function at the non-permissive temperature of 37 °C and recovery at the permissive temperature of 25 °C. We then isolated photosystem I (PSI) and photosystem II (PSII) supercomplexes from cells grown at the non-permissive temperature and determined the PSI structure with high-resolution cryogenic electron microscopy. There were several structural alterations compared with the structures obtained from wild-type cells. Our structural data suggest that the mutant responded by excluding the Lhca2, Lhca9, PsaL, and PsaH subunits. This structural alteration prevents state two transition, where LHCII migrates from PSII to bind to the PSI complex. We propose this as a possible response mechanism triggered by the TSP9 phenotype at the non-permissive temperature.
External linksBiomolecules / PubMed:36979472 / PubMed Central
MethodsEM (single particle)
Resolution2.52 Å
Structure data

14248

7r3k

EMDB-14248, PDB-7r3k:
Chlamydomonas reinhardtii TSP9 mutant small Photosystem I complex
Method: EM (single particle) / Resolution: 2.52 Å

Chemicals

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-CA:
Unknown entry

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol / Β-Cryptoxanthin

ChemComp-C7Z:
(1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-DAO:
LAURIC ACID / Lauric acid

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-SPH:
SPHINGOSINE / Sphingosine

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / Phosphatidic acid

ChemComp-DGA:
DIACYL GLYCEROL / Diglyceride

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-ERG:
ERGOSTEROL / Ergosterol

ChemComp-HOH:
WATER / Water

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPHOTOSYNTHESIS / Photosystem I / Complex / Green algae

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