Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56γ.
Journal, issue, pages	Nat Commun, Vol. 11, Issue 1, Page 5043, Year 2020
Publish date	Oct 7, 2020
Authors	Michał S Barski / Jordan J Minnell / Zuzana Hodakova / Valerie E Pye / Andrea Nans / Peter Cherepanov / Goedele N Maertens /
PubMed Abstract	Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor ...Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor disease, unless a therapy becomes available. A key step in the establishment of infection is the integration of viral genetic material into the host genome, catalysed by the retroviral integrase (IN) enzyme. Here, we use X-ray crystallography and single-particle cryo-electron microscopy to determine the structure of the functional deltaretroviral IN assembled on viral DNA ends and bound to the B56γ subunit of its human host factor, protein phosphatase 2 A. The structure reveals a tetrameric IN assembly bound to two molecules of the phosphatase via a conserved short linear motif. Insight into the deltaretroviral intasome and its interaction with the host will be crucial for understanding the pattern of integration events in infected individuals and therefore bears important clinical implications.
External links	Nat Commun / PubMed:33028863 / PubMed Central
Methods	X-ray diffraction / EM (single particle)
Resolution	1.8 - 3.34 Å
Structure data	PDB-6qbt: Structure of the HTLV-2 integrase catalytic core domain in complex with magnesium (trimeric form) Method: X-RAY DIFFRACTION / Resolution: 2.29 Å PDB-6qbv: Structure of the HTLV-2 integrase catalytic core domain in complex with magnesium (dimeric form) Method: X-RAY DIFFRACTION / Resolution: 2.45 Å PDB-6qbw: Structure of the HTLV-2 integrase catalytic core domain in complex with calcium Method: X-RAY DIFFRACTION / Resolution: 2.4 Å PDB-6tju: X-ray structure of C-terminal domain of human T-cell lymphotropic virus type 1 (HTLV-1) Method: X-RAY DIFFRACTION / Resolution: 1.8 Å PDB-6toq: Crystal structure of a PP2A B56y/HTLV-1 integrase complex Method: X-RAY DIFFRACTION / Resolution: 3.164 Å PDB-7pel: CryoEM structure of simian T-cell lymphotropic virus intasome in complex with PP2A regulatory subunit B56 gamma Method: ELECTRON MICROSCOPY / Resolution: 3.34 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-HOH: WATER / Water ChemComp-CA: Unknown entry ChemComp-ZN: Unknown entry
Source	human t-cell leukemia virus 2 human t-cell leukemia virus type i homo sapiens (human) simian t-lymphotropic virus 1 synthetic construct (others)
Keywords	TRANSFERASE / retrovirus / deltaretrovirus / integration / strand-transfer / nucleotidyltransferase / HTLV-1 / integrase / DNA-binding / SIGNALING PROTEIN / Phosphatase / complex / SLiM / dephosphorylation / cell signalling / motif mimicry / VIRAL PROTEIN / PP2A / B56 / molecular mimicry / host factor