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TitleCryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex at 2.5 Å.
Journal, issue, pagesBiochem J, Vol. 478, Issue 17, Page 3253-3263, Year 2021
Publish dateSep 17, 2021
AuthorsPu Qian / Tristan I Croll / David J K Swainsbury / Pablo Castro-Hartmann / Nigel W Moriarty / Kasim Sader / C Neil Hunter /
PubMed AbstractThe reaction centre light-harvesting 1 (RC-LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM) structure of the RC-LH1 ...The reaction centre light-harvesting 1 (RC-LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM) structure of the RC-LH1 complex from Rhodospirillum rubrum at 2.5 Å resolution, which reveals a unique monomeric bacteriochlorophyll with a phospholipid ligand in the gap between the RC and LH1 complexes. The LH1 complex comprises a circular array of 16 αβ-polypeptide subunits that completely surrounds the RC, with a preferential binding site for a quinone, designated QP, on the inner face of the encircling LH1 complex. Quinols, initially generated at the RC QB site, are proposed to transiently occupy the QP site prior to traversing the LH1 barrier and diffusing to the cytochrome bc1 complex. Thus, the QP site, which is analogous to other such sites in recent cryo-EM structures of RC-LH1 complexes, likely reflects a general mechanism for exporting quinols from the RC-LH1 complex.
External linksBiochem J / PubMed:34402504 / PubMed Central
MethodsEM (single particle)
Resolution2.5 Å
Structure data

13110

7oy8

EMDB-13110, PDB-7oy8:
Cryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex
Method: EM (single particle) / Resolution: 2.5 Å

Chemicals

ChemComp-07D:
Trans-Geranyl BACTERIOCHLOROPHYLL A

ChemComp-CRT:
SPIRILLOXANTHIN

ChemComp-PGW:
(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / phospholipid*YM / Phosphatidylglycerol

ChemComp-CD4:
(2R,5R,11R,14R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate

ChemComp-BPH:
BACTERIOPHEOPHYTIN A / Pheophytin

ChemComp-RQ0:
2-azanyl-5-[(2~{E},6~{E},8~{E},10~{E},12~{E},14~{E},18~{E},22~{E},26~{E},30~{E},34~{E})-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,8,10,12,14,18,22,26,30,34,38-dodecaenyl]-3-methoxy-6-methyl-cyclohexa-2,5-diene-1,4-dione

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

ChemComp-FE:
Unknown entry / Iron

ChemComp-CL:
Unknown entry / Chloride

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-HOH:
WATER / Water

Source
  • rhodospirillum rubrum (strain atcc 11170 / ath 1.1.1 / dsm 467 / lmg 4362 / ncimb 8255 / s1) (bacteria)
KeywordsSTRUCTURAL PROTEIN / photosynthesis / light-harvesting complex / reaction centre / purple bacteria / membrane protein

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