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Structure paper

TitleLetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.
Journal, issue, pagesCell, Vol. 181, Issue 3, Page 653-664.e19, Year 2020
Publish dateApr 30, 2020
AuthorsGeorgia L Isom / Nicolas Coudray / Mark R MacRae / Collin T McManus / Damian C Ekiert / Gira Bhabha /
PubMed AbstractGram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate ...Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 Å cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel sufficiently long to span the periplasm. Lipids bind inside the tunnel, suggesting that it functions as a pathway for lipid transport. Cryo-EM structures in the open and closed states reveal a dynamic tunnel lining, with implications for gating or substrate translocation. Our results support a model in which LetB establishes a physical link between the two membranes and creates a hydrophobic pathway for the translocation of lipids across the periplasm.
External linksCell / PubMed:32359438 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.15 - 3.78 Å
Structure data

20993

6v0c

EMDB-20993: Lipophilic Envelope-spanning Tunnel B (LetB), Map 1
PDB-6v0c: Lipophilic Envelope-spanning Tunnel B (LetB), Model 1
Method: EM (single particle) / Resolution: 3.46 Å

20994

6v0d

EMDB-20994: Lipophilic Envelope-spanning Tunnel B (LetB), Map 2
PDB-6v0d: Lipophilic Envelope-spanning Tunnel B (LetB), Model 2
Method: EM (single particle) / Resolution: 3.49 Å

20995

6v0e

EMDB-20995: Lipophilic Envelope-spanning Tunnel B (LetB), Map 3
PDB-6v0e: Lipophilic Envelope-spanning Tunnel B (LetB), Model 3
Method: EM (single particle) / Resolution: 3.06 Å

20996

6v0f

EMDB-20996: Lipophilic Envelope-spanning Tunnel B (LetB), Map 4
PDB-6v0f: Lipophilic Envelope-spanning Tunnel B (LetB), Model 4
Method: EM (single particle) / Resolution: 2.96 Å

20997

6v0g

EMDB-20997: Lipophilic Envelope-spanning Tunnel B (LetB), Map 5
PDB-6v0g: Lipophilic Envelope-spanning Tunnel B (LetB), Model 5
Method: EM (single particle) / Resolution: 3.03 Å

20998

6v0h

EMDB-20998: Lipophilic Envelope-spanning Tunnel B (LetB), Map 6
PDB-6v0h: Lipophilic Envelope-spanning Tunnel B (LetB), Model 6
Method: EM (single particle) / Resolution: 3.6 Å

20999

6v0i

EMDB-20999: Lipophilic Envelope-spanning Tunnel B (LetB), Map 7
PDB-6v0i: Lipophilic Envelope-spanning Tunnel B (LetB), Model 7
Method: EM (single particle) / Resolution: 3.43 Å

21000

6v0j

EMDB-21000: Lipophilic Envelope-spanning Tunnel B (LetB), Map 8
PDB-6v0j: Lipophilic Envelope-spanning Tunnel B (LetB), Model 8
Method: EM (single particle) / Resolution: 3.78 Å

PDB-6vci:
Lipophilic envelope-spanning tunnel protein (LetB), domains MCE2-MCE3
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Escherichia coli K-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsLIPID TRANSPORT / bacterial cell envelope / MCE / YebT / conformational dynamics / LetB

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